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The map label for this gene is pflA [H]

Identifier: 52785882

GI number: 52785882

Start: 2077048

End: 2077806

Strand: Reverse

Name: pflA [H]

Synonym: BLi02131

Alternate gene names: 52785882

Gene position: 2077806-2077048 (Counterclockwise)

Preceding gene: 52785883

Following gene: 52785881

Centisome position: 49.21

GC content: 50.33

Gene sequence:

>759_bases
ATGGATGGAAATATTCATTCGATCGAAACATTCGGCACCGTTGACGGTCCAGGCATCAGGTATGTCGTCTTCACACAAGG
CTGCCTGATGCGCTGTCAATTTTGCCATAATGCTGATACTTGGGAAATCGGAACCGGAAAACAAATGACGGTTTCCGAAA
TCGTTCAGGATGTCCAGCATTATCTCCCGTTTATTCAATCATCGGGCGGAGGCATCACCGTGAGCGGAGGCGAGCCGCTT
TTACAACTGCCGTTTTTAATCGAGCTGTTCAAAGCATGCAAAAGCCTCGGCATTCACACGGCACTCGATTCGTCCGGCGG
ATGCTATTCGGCTGCGCCGGCATTTCAAGAGCAGATCAAAGAACTGATCCAGTATACAGACCTTGTTTTGCTTGACCTCA
AGCATCACAACAGAAAAAAACATATCAACCTGACAGGAATGCCGAATGACCACATTTTAGAATTTGCCCGGTTTCTCGCT
GAACATCAAGTTCCCGTCTGGATCCGCCACGTACTGGTTCCGGGGATCTCCGATATCGATGCCGATTTAACGGCCCTCGG
CACGTTTATCGGCACGCTTGCGAACGTTCAGAAGGTGGAGGTTCTTCCTTATCACAAGCTCGGCGTCTACAAATGGGAAG
CGCTTGGCCTGGATTATCCGTTAAAAGGGGTTGAACCGCCAAGTGCCGACAGGGCCGAAAATGCGTACAGACTGCTCACC
GCACACTTGCAAGGCGGATCCTTGCTGCAAGAGACATAA

Upstream 100 bases:

>100_bases
GCAGTTAGACGTCATCAGCAGAACCTTCCATGAATCGATGTAGCGAGCAATAAGCGGAGCGCGACCAAGGCGCTCCGCCT
CATAAAAAGAGGTGATCTCC

Downstream 100 bases:

>100_bases
ACGCTTTTGCCGGACAGACTTTTTCTAACAAGAAGCTGTCCTTTTTGTCTTACCTCACAACTGTATAAAAAATCCGTTTC
TTTACAATAGTAAGATGAGA

Product: hypothetical protein

Products: NA

Alternate protein names: PFL-activating enzyme [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MDGNIHSIETFGTVDGPGIRYVVFTQGCLMRCQFCHNADTWEIGTGKQMTVSEIVQDVQHYLPFIQSSGGGITVSGGEPL
LQLPFLIELFKACKSLGIHTALDSSGGCYSAAPAFQEQIKELIQYTDLVLLDLKHHNRKKHINLTGMPNDHILEFARFLA
EHQVPVWIRHVLVPGISDIDADLTALGTFIGTLANVQKVEVLPYHKLGVYKWEALGLDYPLKGVEPPSADRAENAYRLLT
AHLQGGSLLQET

Sequences:

>Translated_252_residues
MDGNIHSIETFGTVDGPGIRYVVFTQGCLMRCQFCHNADTWEIGTGKQMTVSEIVQDVQHYLPFIQSSGGGITVSGGEPL
LQLPFLIELFKACKSLGIHTALDSSGGCYSAAPAFQEQIKELIQYTDLVLLDLKHHNRKKHINLTGMPNDHILEFARFLA
EHQVPVWIRHVLVPGISDIDADLTALGTFIGTLANVQKVEVLPYHKLGVYKWEALGLDYPLKGVEPPSADRAENAYRLLT
AHLQGGSLLQET
>Mature_252_residues
MDGNIHSIETFGTVDGPGIRYVVFTQGCLMRCQFCHNADTWEIGTGKQMTVSEIVQDVQHYLPFIQSSGGGITVSGGEPL
LQLPFLIELFKACKSLGIHTALDSSGGCYSAAPAFQEQIKELIQYTDLVLLDLKHHNRKKHINLTGMPNDHILEFARFLA
EHQVPVWIRHVLVPGISDIDADLTALGTFIGTLANVQKVEVLPYHKLGVYKWEALGLDYPLKGVEPPSADRAENAYRLLT
AHLQGGSLLQET

Specific function: Activation of pyruvate formate-lyase under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=49.5833333333333, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1790389, Length=258, Percent_Identity=27.906976744186, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1790839, Length=256, Percent_Identity=29.6875, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI226510931, Length=304, Percent_Identity=27.6315789473684, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27790; Mature: 27790

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGNIHSIETFGTVDGPGIRYVVFTQGCLMRCQFCHNADTWEIGTGKQMTVSEIVQDVQH
CCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHH
YLPFIQSSGGGITVSGGEPLLQLPFLIELFKACKSLGIHTALDSSGGCYSAAPAFQEQIK
HHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHH
ELIQYTDLVLLDLKHHNRKKHINLTGMPNDHILEFARFLAEHQVPVWIRHVLVPGISDID
HHHHHHHEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
ADLTALGTFIGTLANVQKVEVLPYHKLGVYKWEALGLDYPLKGVEPPSADRAENAYRLLT
HHHHHHHHHHHHHHCCCEEEECCCHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
AHLQGGSLLQET
HHCCCCCHHCCC
>Mature Secondary Structure
MDGNIHSIETFGTVDGPGIRYVVFTQGCLMRCQFCHNADTWEIGTGKQMTVSEIVQDVQH
CCCCCCEEEECCCCCCCCEEEEEECCCHHHHHHHHCCCCCEECCCCCCCCHHHHHHHHHH
YLPFIQSSGGGITVSGGEPLLQLPFLIELFKACKSLGIHTALDSSGGCYSAAPAFQEQIK
HHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHH
ELIQYTDLVLLDLKHHNRKKHINLTGMPNDHILEFARFLAEHQVPVWIRHVLVPGISDID
HHHHHHHEEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCC
ADLTALGTFIGTLANVQKVEVLPYHKLGVYKWEALGLDYPLKGVEPPSADRAENAYRLLT
HHHHHHHHHHHHHHCCCEEEECCCHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHH
AHLQGGSLLQET
HHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA