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The map label for this gene is ymcB [H]

Identifier: 52785683

GI number: 52785683

Start: 1885354

End: 1886883

Strand: Direct

Name: ymcB [H]

Synonym: BLi01925

Alternate gene names: 52785683

Gene position: 1885354-1886883 (Clockwise)

Preceding gene: 52785682

Following gene: 52785684

Centisome position: 44.65

GC content: 47.58

Gene sequence:

>1530_bases
ATGAATGAGAAACAGCGCATTGAAAGCGGACAGGTCAATCCATCGGACAAAAAATCCGAGAAGGATTACAGCAAGTACTT
CGAAGCTGTTTATGTTCCGCCTTCATTAAAAGATGCGAAAAAACGCGGCAAAGAAGAGGTTAAATACCATAACGATTTTA
AAATCTCAGAGCAGTTCAGAGGCATGGGCGAAGGACGGAAGTTTTACATCCGCACGTACGGCTGCCAGATGAATGAGCAC
GATACCGAAGTGATGGCAGGGATTTTCATGGCGCTCGGCTATGAACCGACCGACTCGACGGAAGATGCAAACGTCATTTT
GCTCAACACGTGTGCGATCCGGGAAAACGCGGAAAACAAAGTGTTTGGCGAAATCGGCCATTTAAAAGCTTTGAAAAAAG
ACAATCCGGATTTGATCCTCGGAGTCTGCGGATGTATGTCCCAGGAAGAATCAGTCGTCAACCGGATTTTAAAGAAACAT
CCGTTCGTCGATCTGATTTTCGGTACACACAATATCCACCGCTTGCCCGAACTTTTGTCAGAGGCTTATCTGTCAAAAGA
AATGGTCATCGAAGTGTGGTCTAAAGAAGGCGATGTCATTGAAAACCTTCCGAAAGTCCGCACCGGCAAAATTAAAGGCT
GGGTCAACATCATGTACGGCTGCGACAAGTTCTGTACGTACTGCATCGTGCCGTACACAAGAGGAAAAGAACGAAGCAGA
CGTCCTGAAGACATCATCCAGGAAGTGAGAAGGCTTGCGGCTGAAGGCTACAAAGAAATCACGCTTCTCGGACAGAACGT
CAACGCCTACGGCAAAGATTTTGAGGATATGGAATACGGACTCGGCCACTTGATGGATGAACTCCGCAAAATCGACATTC
CGCGAATCCGCTTCACGACAAGCCATCCGCGCGATTTTGACGACCATCTGATCGAGGTGCTCGCAAAAGGCGGAAACCTT
CTGGACCACATCCATCTGCCTGTCCAGTCCGGAAGCTCGGATGTCTTGAAACTGATGGCGCGCAAATACGACCGGGAGCG
CTATCTTGAGCTTGTCGCCAAAATTAAAAAAGCGATGCCGAACGCATCCTTAACAACGGACATTATCGTCGGCTTCCCGA
ACGAAACAGACGAGCAATTTGAAGAAACGCTCTCTTTATACAGAGAGGTTGAATTTGACAGCGCCTATACGTTCATCTAC
TCGCCTCGCGAAGGAACGCCTGCTGCCAAAATGAAAGACAATGTTCCGATGCGCGTGAAAAAAGAACGGCTTCAGCGGTT
AAATGAAGTGGTCAACGAAATTTCCGCGAAGAAAATGAAGGAATATGAGGGGCAGGTTGTCGAAGTGTTAGTAGAGGGTG
AAAGTAAAAACAACCCTGATATCCTTGCCGGCTATACACGGAAAAACAAGCTTGTCAACTTCAAAGGACCAAAAGAAGCG
ATCGGCCAGCTTGTCAACGTGAAAATCCACCAGGCGAAAACTTGGTCGCTTGACGGGGAAATGGTAGGAGAAGCAATCGA
GGTGAAGTAA

Upstream 100 bases:

>100_bases
TCATTTTCAGTTAGTGTACAATGATGGGTTCTGGGTTGTGCTGACAACCGAGAACCTTTATACTGAAGAGTGGAGCTTTG
TTTGAAAGGAGATCAAAAAC

Downstream 100 bases:

>100_bases
AGTGACGCTTTATACGAAAAAAGAGATTGTTGCAAAAGCGCGGGAGCTCGCAAAAATGATTGCAGAAACGGAAGAAGTCG
ACTTTTTCAAAAAAGCGGAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MNEKQRIESGQVNPSDKKSEKDYSKYFEAVYVPPSLKDAKKRGKEEVKYHNDFKISEQFRGMGEGRKFYIRTYGCQMNEH
DTEVMAGIFMALGYEPTDSTEDANVILLNTCAIRENAENKVFGEIGHLKALKKDNPDLILGVCGCMSQEESVVNRILKKH
PFVDLIFGTHNIHRLPELLSEAYLSKEMVIEVWSKEGDVIENLPKVRTGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKIDIPRIRFTTSHPRDFDDHLIEVLAKGGNL
LDHIHLPVQSGSSDVLKLMARKYDRERYLELVAKIKKAMPNASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIY
SPREGTPAAKMKDNVPMRVKKERLQRLNEVVNEISAKKMKEYEGQVVEVLVEGESKNNPDILAGYTRKNKLVNFKGPKEA
IGQLVNVKIHQAKTWSLDGEMVGEAIEVK

Sequences:

>Translated_509_residues
MNEKQRIESGQVNPSDKKSEKDYSKYFEAVYVPPSLKDAKKRGKEEVKYHNDFKISEQFRGMGEGRKFYIRTYGCQMNEH
DTEVMAGIFMALGYEPTDSTEDANVILLNTCAIRENAENKVFGEIGHLKALKKDNPDLILGVCGCMSQEESVVNRILKKH
PFVDLIFGTHNIHRLPELLSEAYLSKEMVIEVWSKEGDVIENLPKVRTGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKIDIPRIRFTTSHPRDFDDHLIEVLAKGGNL
LDHIHLPVQSGSSDVLKLMARKYDRERYLELVAKIKKAMPNASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIY
SPREGTPAAKMKDNVPMRVKKERLQRLNEVVNEISAKKMKEYEGQVVEVLVEGESKNNPDILAGYTRKNKLVNFKGPKEA
IGQLVNVKIHQAKTWSLDGEMVGEAIEVK
>Mature_509_residues
MNEKQRIESGQVNPSDKKSEKDYSKYFEAVYVPPSLKDAKKRGKEEVKYHNDFKISEQFRGMGEGRKFYIRTYGCQMNEH
DTEVMAGIFMALGYEPTDSTEDANVILLNTCAIRENAENKVFGEIGHLKALKKDNPDLILGVCGCMSQEESVVNRILKKH
PFVDLIFGTHNIHRLPELLSEAYLSKEMVIEVWSKEGDVIENLPKVRTGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
RPEDIIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKIDIPRIRFTTSHPRDFDDHLIEVLAKGGNL
LDHIHLPVQSGSSDVLKLMARKYDRERYLELVAKIKKAMPNASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIY
SPREGTPAAKMKDNVPMRVKKERLQRLNEVVNEISAKKMKEYEGQVVEVLVEGESKNNPDILAGYTRKNKLVNFKGPKEA
IGQLVNVKIHQAKTWSLDGEMVGEAIEVK

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=37.0600414078675, Blast_Score=303, Evalue=2e-82,
Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=37.0600414078675, Blast_Score=303, Evalue=3e-82,
Organism=Homo sapiens, GI93277076, Length=454, Percent_Identity=31.057268722467, Blast_Score=189, Evalue=6e-48,
Organism=Escherichia coli, GI1786882, Length=440, Percent_Identity=43.8636363636364, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=28.6031042128603, Blast_Score=171, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI17553146, Length=475, Percent_Identity=35.1578947368421, Blast_Score=273, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI71996771, Length=299, Percent_Identity=31.438127090301, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356207, Length=432, Percent_Identity=39.1203703703704, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=30.1762114537445, Blast_Score=184, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 58295; Mature: 58295

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEKQRIESGQVNPSDKKSEKDYSKYFEAVYVPPSLKDAKKRGKEEVKYHNDFKISEQFR
CCCHHHCCCCCCCCCCCCCHHHHHHHHHEEECCCCHHHHHHCCHHHCCCCCCCCHHHHHC
GMGEGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDSTEDANVILLNTCAIRENAENK
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
VFGEIGHLKALKKDNPDLILGVCGCMSQEESVVNRILKKHPFVDLIFGTHNIHRLPELLS
HHHHHHHHHHHCCCCCCEEEEEHHCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
EAYLSKEMVIEVWSKEGDVIENLPKVRTGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKIDIPRIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEC
SHPRDFDDHLIEVLAKGGNLLDHIHLPVQSGSSDVLKLMARKYDRERYLELVAKIKKAMP
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVK
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNEVVNEISAKKMKEYEGQVVEVLVEGESKNNPDILAGYTRKNKLVNFKGPKEA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCEEECCCHHHH
IGQLVNVKIHQAKTWSLDGEMVGEAIEVK
HHHHHCEEEECCEEECCCHHHHCCHHCCC
>Mature Secondary Structure
MNEKQRIESGQVNPSDKKSEKDYSKYFEAVYVPPSLKDAKKRGKEEVKYHNDFKISEQFR
CCCHHHCCCCCCCCCCCCCHHHHHHHHHEEECCCCHHHHHHCCHHHCCCCCCCCHHHHHC
GMGEGRKFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDSTEDANVILLNTCAIRENAENK
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCCCCCC
VFGEIGHLKALKKDNPDLILGVCGCMSQEESVVNRILKKHPFVDLIFGTHNIHRLPELLS
HHHHHHHHHHHCCCCCCEEEEEHHCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHH
EAYLSKEMVIEVWSKEGDVIENLPKVRTGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSR
HHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCCCC
RPEDIIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKIDIPRIRFTT
CHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEC
SHPRDFDDHLIEVLAKGGNLLDHIHLPVQSGSSDVLKLMARKYDRERYLELVAKIKKAMP
CCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NASLTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVK
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCCCHHHH
KERLQRLNEVVNEISAKKMKEYEGQVVEVLVEGESKNNPDILAGYTRKNKLVNFKGPKEA
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEECCCCCEEECCCHHHH
IGQLVNVKIHQAKTWSLDGEMVGEAIEVK
HHHHHCEEEECCEEECCCHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA