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The map label for this gene is ymfA [H]

Identifier: 52785662

GI number: 52785662

Start: 1862246

End: 1863910

Strand: Direct

Name: ymfA [H]

Synonym: BLi01903

Alternate gene names: 52785662

Gene position: 1862246-1863910 (Clockwise)

Preceding gene: 52785661

Following gene: 52785663

Centisome position: 44.1

GC content: 46.79

Gene sequence:

>1665_bases
TTGAAAAAAAATACAGAAAATATTAGAATCATCGCCTTAGGAGGCGTCGGAGAGATCGGAAAAAACCTCTATGTCATTGA
AATCGATTCAGACATATTCGTGGTTGACGCCGGCCTCATGCATCCAGAAAATGAAATGCTGGGTATCGATGTGGTCATCC
CTGACATTTCTTATTTAATTGAACGATCTGACCGCGTAAAGGCGATTTTTCTCACACACGGGCATGAAGAAAACATCGGC
GGCGTATTTTACTGCCTGAACAAGCTTTCTGTGCCTGTTTACGGAACGAAGCTGACGCTTGCCCTTTTAAAGGAAACGCT
GAAGCAGTACGGTGTAAACAAAAAGCCCGATTTAAGAGAAATTCATTCAAAATCCGTTATTACATTTGAATCGACAAAGG
TGTCATTCTTCAAAACAAATCACAGCATCCCGGATTCCGTCGGTGTCAGCTTTAAAACATCCCTCGGATCCATCGTGTGC
ACAGGCGATTTTAAATTTGACCAGACACCTGTGTTAAACCAGACAAGCGACATCGGCGAAATTGCCAAAATCGGCAACAG
CGGTGTGCTGTGCCTGCTTTCCGACAGCGCCAATGCCGAGAAGCCGGGCTATACGCCTTCAGAAGCACTCGTTGGCGGAG
AGATATCCGATGTGATGTACAACGCCGAAAACCGCGTGATTATTGCCGTGTTCGCGTCTAACTTTAACCGGATTCAGCAG
GTGATTGATGCCGCGCTGCAAAACGGCAGGAAGCTTGCAGTTTCAGGGAAAAATATTTCGGCGATACTCCAGCTGGCGAT
GAAACTCGGGTATGTCACGGCAGATGAAGATATGTTCATCCCTGTCCAAGAAGTCAAAAAGTATCCAAAAAGAGAAGTCG
CGATTTTGACAGGGGACAACCATGGAGAGCCGCTCGCCGCATTGACCAGGATCGCAAAACAAGGGCATAAGCAGCTTGAC
ATTGAGGAAGGGGATACCGTCCTGATCGCATCCTCCCCTATCCCGGGTCAGGAGCTTGTTTACTCCAAAGCGGTCGATCT
CTTAACGAGAGCGGGCGCCCAGGTCGTATTCGCACAAAAACGCGTCCATGTATCAGGCCATGGCTGTCAAGAAGAGCTGA
AGCTGATGCTCAACTTGCTGAAGCCGAAGTATTTAATCCCCGTAAACGGTGAATACAGAATGCAAAAAGCGCATTCCAAG
CTTGCCGAAGAAATCGGCATGAAACGGAGCGACATTTTCCTGATTGAAAAAGGAGATGTCGTGGAATTCCGCGGACAAAA
CGTTAAAATCGGCGATAAAGTACACTCAGGCAATATATTGATCGACGGACTCGGCGTCGGCGATATCGGCAACATCGTGC
TCAGAGACCGCCGTCTGCTATCCCAGGACGGAATCTTAATTGTTGTGATTACCCTTGATAAAAAGAAAAAACATCTAGTT
TCAGGACCCGAAATTATCACGAGAGGTTTTGTCTATGTCAGAGAATCTGAACAGCTGATCGTTGAAGCGACAGAGCTTGT
CAGGGAACTCGTCAACGAAAGCACAGAAAACGCCGTCGTCGAATGGTCCGCCTTAAAACAGACGATGCGTGAGGCATTAA
ACCAGTTCCTATACGAAAAAACGAAACGAAAACCAATGATCATTCCAATCATCATGGAAGTATAA

Upstream 100 bases:

>100_bases
GTTTGTCATATACATAATGGTTCTTGTTTTCTAAATTTAGATTCAGGTATAATAGGGACAAGTGATGATGGACGGGTACT
TAGATTAGGAGGATTACAAT

Downstream 100 bases:

>100_bases
AAAACCAAGAGACTCCTCTTGGTTTTTTTTCATGCCTTTTGCGTTGAATGAAAAAAACGCCATTGTCCATAATACAAAAG
AAACTCGAGAAAGAGGTGAG

Product: YmfA

Products: NA

Alternate protein names: RNase J2 [H]

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MKKNTENIRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERSDRVKAIFLTHGHEENIG
GVFYCLNKLSVPVYGTKLTLALLKETLKQYGVNKKPDLREIHSKSVITFESTKVSFFKTNHSIPDSVGVSFKTSLGSIVC
TGDFKFDQTPVLNQTSDIGEIAKIGNSGVLCLLSDSANAEKPGYTPSEALVGGEISDVMYNAENRVIIAVFASNFNRIQQ
VIDAALQNGRKLAVSGKNISAILQLAMKLGYVTADEDMFIPVQEVKKYPKREVAILTGDNHGEPLAALTRIAKQGHKQLD
IEEGDTVLIASSPIPGQELVYSKAVDLLTRAGAQVVFAQKRVHVSGHGCQEELKLMLNLLKPKYLIPVNGEYRMQKAHSK
LAEEIGMKRSDIFLIEKGDVVEFRGQNVKIGDKVHSGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKKKKHLV
SGPEIITRGFVYVRESEQLIVEATELVRELVNESTENAVVEWSALKQTMREALNQFLYEKTKRKPMIIPIIMEV

Sequences:

>Translated_554_residues
MKKNTENIRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERSDRVKAIFLTHGHEENIG
GVFYCLNKLSVPVYGTKLTLALLKETLKQYGVNKKPDLREIHSKSVITFESTKVSFFKTNHSIPDSVGVSFKTSLGSIVC
TGDFKFDQTPVLNQTSDIGEIAKIGNSGVLCLLSDSANAEKPGYTPSEALVGGEISDVMYNAENRVIIAVFASNFNRIQQ
VIDAALQNGRKLAVSGKNISAILQLAMKLGYVTADEDMFIPVQEVKKYPKREVAILTGDNHGEPLAALTRIAKQGHKQLD
IEEGDTVLIASSPIPGQELVYSKAVDLLTRAGAQVVFAQKRVHVSGHGCQEELKLMLNLLKPKYLIPVNGEYRMQKAHSK
LAEEIGMKRSDIFLIEKGDVVEFRGQNVKIGDKVHSGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKKKKHLV
SGPEIITRGFVYVRESEQLIVEATELVRELVNESTENAVVEWSALKQTMREALNQFLYEKTKRKPMIIPIIMEV
>Mature_554_residues
MKKNTENIRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLIERSDRVKAIFLTHGHEENIG
GVFYCLNKLSVPVYGTKLTLALLKETLKQYGVNKKPDLREIHSKSVITFESTKVSFFKTNHSIPDSVGVSFKTSLGSIVC
TGDFKFDQTPVLNQTSDIGEIAKIGNSGVLCLLSDSANAEKPGYTPSEALVGGEISDVMYNAENRVIIAVFASNFNRIQQ
VIDAALQNGRKLAVSGKNISAILQLAMKLGYVTADEDMFIPVQEVKKYPKREVAILTGDNHGEPLAALTRIAKQGHKQLD
IEEGDTVLIASSPIPGQELVYSKAVDLLTRAGAQVVFAQKRVHVSGHGCQEELKLMLNLLKPKYLIPVNGEYRMQKAHSK
LAEEIGMKRSDIFLIEKGDVVEFRGQNVKIGDKVHSGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKKKKHLV
SGPEIITRGFVYVRESEQLIVEATELVRELVNESTENAVVEWSALKQTMREALNQFLYEKTKRKPMIIPIIMEV

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Appears to have a limited effect on 16S rRNA maturation, despite its similarity to rnjA [H]

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 61157; Mature: 61157

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNTENIRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLI
CCCCCCCEEEEEECCCHHHCCCEEEEEECCCEEEEECCCCCCCCCEEEEEEEECCHHHHH
ERSDRVKAIFLTHGHEENIGGVFYCLNKLSVPVYGTKLTLALLKETLKQYGVNKKPDLRE
CCCCCEEEEEEECCCCCCCCEEEEEHHHCCCCEECHHHHHHHHHHHHHHCCCCCCCCHHH
IHSKSVITFESTKVSFFKTNHSIPDSVGVSFKTSLGSIVCTGDFKFDQTPVLNQTSDIGE
HCCCCEEEEECCEEEEEECCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHH
IAKIGNSGVLCLLSDSANAEKPGYTPSEALVGGEISDVMYNAENRVIIAVFASNFNRIQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCHHHHEECCCCCEEEEEECCCHHHHHH
VIDAALQNGRKLAVSGKNISAILQLAMKLGYVTADEDMFIPVQEVKKYPKREVAILTGDN
HHHHHHHCCCEEEEECCCHHHHHHHHHHHCEEECCCCEEEEHHHHHCCCCCEEEEEECCC
HGEPLAALTRIAKQGHKQLDIEEGDTVLIASSPIPGQELVYSKAVDLLTRAGAQVVFAQK
CCCHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
RVHVSGHGCQEELKLMLNLLKPKYLIPVNGEYRMQKAHSKLAEEIGMKRSDIFLIEKGDV
EEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCE
VEFRGQNVKIGDKVHSGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKKKKHLV
EEECCCEEEECCEEECCCEEEEECCCCCCCHHHHHCCHHHCCCCEEEEEEEECCCCHHHC
SGPEIITRGFVYVRESEQLIVEATELVRELVNESTENAVVEWSALKQTMREALNQFLYEK
CCCHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
TKRKPMIIPIIMEV
HCCCCEEEEEEEEC
>Mature Secondary Structure
MKKNTENIRIIALGGVGEIGKNLYVIEIDSDIFVVDAGLMHPENEMLGIDVVIPDISYLI
CCCCCCCEEEEEECCCHHHCCCEEEEEECCCEEEEECCCCCCCCCEEEEEEEECCHHHHH
ERSDRVKAIFLTHGHEENIGGVFYCLNKLSVPVYGTKLTLALLKETLKQYGVNKKPDLRE
CCCCCEEEEEEECCCCCCCCEEEEEHHHCCCCEECHHHHHHHHHHHHHHCCCCCCCCHHH
IHSKSVITFESTKVSFFKTNHSIPDSVGVSFKTSLGSIVCTGDFKFDQTPVLNQTSDIGE
HCCCCEEEEECCEEEEEECCCCCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCHHH
IAKIGNSGVLCLLSDSANAEKPGYTPSEALVGGEISDVMYNAENRVIIAVFASNFNRIQQ
HHHCCCCCEEEEEECCCCCCCCCCCCCHHCCCCCHHHHEECCCCCEEEEEECCCHHHHHH
VIDAALQNGRKLAVSGKNISAILQLAMKLGYVTADEDMFIPVQEVKKYPKREVAILTGDN
HHHHHHHCCCEEEEECCCHHHHHHHHHHHCEEECCCCEEEEHHHHHCCCCCEEEEEECCC
HGEPLAALTRIAKQGHKQLDIEEGDTVLIASSPIPGQELVYSKAVDLLTRAGAQVVFAQK
CCCHHHHHHHHHHCCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEEE
RVHVSGHGCQEELKLMLNLLKPKYLIPVNGEYRMQKAHSKLAEEIGMKRSDIFLIEKGDV
EEEECCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCE
VEFRGQNVKIGDKVHSGNILIDGLGVGDIGNIVLRDRRLLSQDGILIVVITLDKKKKHLV
EEECCCEEEECCEEECCCEEEEECCCCCCCHHHHHCCHHHCCCCEEEEEEEECCCCHHHC
SGPEIITRGFVYVRESEQLIVEATELVRELVNESTENAVVEWSALKQTMREALNQFLYEK
CCCHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHH
TKRKPMIIPIIMEV
HCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]