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The map label for this gene is infB

Identifier: 52785647

GI number: 52785647

Start: 1846826

End: 1848976

Strand: Direct

Name: infB

Synonym: BLi01888

Alternate gene names: 52785647

Gene position: 1846826-1848976 (Clockwise)

Preceding gene: 52785646

Following gene: 52785648

Centisome position: 43.74

GC content: 47.56

Gene sequence:

>2151_bases
ATGGCTAAAATGAGAGTTTATGAATATGCAAAAGCCATAAATGTTTCAAGTAAGGATATTATCGCGGCATTGAAAGATAT
GAATATCGAAGTCAATAACCATATGGCGATGATCGAAGAAAATGCGATCAAACAGCTCGATCAAAAATTCAAGAAGAGCG
CTAAACCTGCGGGAAATAAAAAGGATGCCGGTACAGGCGGACAAAAGCCGCAGCAAGAAGCCGCAAAACTGAATGCCGGA
AGTAAGCCAAACAAAAATCGAGACGGAAAGAAGAATAACGTGCAGAATACTCAATTTAACAATAAAAACAAGAAGAAAAA
CAACAACAACAAAAAGAACAAACGCGGTAAAAATCATCAATCTCCTCAGGAGAAGCAGTTCAAGCCGAAAAAAGAACTGC
CTGAAAAAATCGAATTTACCAACTCAATGTCGGTCGGCGCGCTTGCCGAAGAGCTCGGAAAAGAACCGTCAGAAATCATT
AAAAAATTAATGCTTCTCGGCATCATGGCGACGATCAACCAAGATCTTGACAAAGATACCGTTGAGCTGATCGCTTCAGA
ATACGGCGTCGAAGTCGAAGAAGTGATCGTCCATGAAGAAACCGAATTCGAAAAATACGAAGAGCCGGATAACGAAGAAG
ACCTCGAAATCCGTCCTCCTGTCGTAACGATCATGGGTCACGTCGACCACGGAAAAACAACGCTTCTCGACAGCATCAGA
AAAACGAAGGTCGTTGAAGGCGAAGCCGGAGGCATCACTCAGCATATCGGCGCTTATCAGATCGAGGAAAACGGCAAGAA
GATTACCTTCCTCGACACTCCGGGCCACGCCGCGTTTACAACGATGCGCGCACGCGGTGCGGAAGTTACGGACATTACGA
TTCTCGTCGTAGCTGCAGATGACGGGGTCATGCCGCAGACGGTTGAAGCGATCAACCATGCGAAAGCGGCTGAAGTGCCG
ATCATCGTCGCTGTCAACAAAATCGACAAACCGACGGCAAACCCTGACCGCGTGATGCAGGAGCTGACTGAACACGGTCT
TGTTCCTGAAGCATGGGGAGGCGAGACGATTTTTGTTCCTCTTTCCGCACTTTCAGGAGAGGGAATCGATGAGCTGATCG
AAATGATCCTGCTCGTCAGCGAAGTCGAGGAGCTCAAAGCAAATCCGAACCGCAGGGCAAAAGGAACGGTCATCGAAGCC
GAGCTTGATAAAGGTAAAGGTTCTGTCGCAACGTTGCTCGTTCAAAACGGAACGCTTCACGTCGGAGATCCGATCGTCGT
CGGGAACACTTTCGGACGCGTTCGTGCGATGGTCAATGATGTCGGCAGACGCGTGAAATCGGCGGGACCGTCAACTCCTG
TTGAGATCACCGGGTTGAACGAAGTTCCGAACGCAGGGGATCAGTTCCTTGTGTTCAAAGACGAAAAAACAGCCCGTTCC
GTCGGCGAAGCGCGCGCTACCAAACAGCTTGAAGAACAGCGCAGCGATAAAGCGAAGCTCAGCCTTGATGACTTGTTTGA
ACAGATTAAACAAGGCGATGTCAAAGAAATTAACCTCGTCGTAAAAGCCGATGTTCAAGGCTCTGTTGAAGCGCTGGCCG
CAGCTTTGCAAAAAATTGAGGTCGAAGGCGTCAGAGTCAAAATCATCCACACAGGCGTAGGTGCGATTACCGAATCAGAC
ATTATTTTAGCTTCTGCATCAAATGCAATCGTGATCGGGTTTAATGTCAGACCTGACGGAAATGCTAAGAGTACCGCCGA
AGCCGAAAATGTGGATATCCGCCTCCACCGCATCATTTATAAAGTCATCGATGAGATCGAAGCAGCCATGAAAGGCATGC
TTGATCCTGAGTATGAAGAAAAAGTGATCGGCATGGTTGAAGTCCGTCAAACATTCAAAGTCTCTAAAATCGGAACGATT
GCCGGAGGCTATGTAACGGAAGGTACGATCACGCGCGACAGCGGAATCCGCTTGATCCGCGACGGCGTCGTCATCTTTGA
AGGGGAAGTCGATGTGCTGAAACGCTTTAAAGATGATGTCAAAGAAGTTTCACAAGGCTATGAATGTGGTATCACCATTA
AGAAATACAACGACATACGCGAAGGCGATATGATCGAAGCGTATGTCATGCAAGAAATTGAAAGAAAGTGA

Upstream 100 bases:

>100_bases
CAATCGGCAAAGAATCCCGTGTGGTTGTTGCTGTCACTGATCAAGGTTTTGCGAATACGTTGCTCCGAAAGCTCGATTAA
TTTTTTTTGGGGGTGAACGA

Downstream 100 bases:

>100_bases
TCGGTTTCGTTGAATGCGAATGCATCATTTACGATGCATCATCACTCAAGGAAAAGCGGGCGGTGCTGAAGCGGATCATC
ACAAGAATCCGCGCCAAGTT

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MAKMRVYEYAKAINVSSKDIIAALKDMNIEVNNHMAMIEENAIKQLDQKFKKSAKPAGNKKDAGTGGQKPQQEAAKLNAG
SKPNKNRDGKKNNVQNTQFNNKNKKKNNNNKKNKRGKNHQSPQEKQFKPKKELPEKIEFTNSMSVGALAEELGKEPSEII
KKLMLLGIMATINQDLDKDTVELIASEYGVEVEEVIVHEETEFEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIR
KTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAADDGVMPQTVEAINHAKAAEVP
IIVAVNKIDKPTANPDRVMQELTEHGLVPEAWGGETIFVPLSALSGEGIDELIEMILLVSEVEELKANPNRRAKGTVIEA
ELDKGKGSVATLLVQNGTLHVGDPIVVGNTFGRVRAMVNDVGRRVKSAGPSTPVEITGLNEVPNAGDQFLVFKDEKTARS
VGEARATKQLEEQRSDKAKLSLDDLFEQIKQGDVKEINLVVKADVQGSVEALAAALQKIEVEGVRVKIIHTGVGAITESD
IILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIYKVIDEIEAAMKGMLDPEYEEKVIGMVEVRQTFKVSKIGTI
AGGYVTEGTITRDSGIRLIRDGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDMIEAYVMQEIERK

Sequences:

>Translated_716_residues
MAKMRVYEYAKAINVSSKDIIAALKDMNIEVNNHMAMIEENAIKQLDQKFKKSAKPAGNKKDAGTGGQKPQQEAAKLNAG
SKPNKNRDGKKNNVQNTQFNNKNKKKNNNNKKNKRGKNHQSPQEKQFKPKKELPEKIEFTNSMSVGALAEELGKEPSEII
KKLMLLGIMATINQDLDKDTVELIASEYGVEVEEVIVHEETEFEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIR
KTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAADDGVMPQTVEAINHAKAAEVP
IIVAVNKIDKPTANPDRVMQELTEHGLVPEAWGGETIFVPLSALSGEGIDELIEMILLVSEVEELKANPNRRAKGTVIEA
ELDKGKGSVATLLVQNGTLHVGDPIVVGNTFGRVRAMVNDVGRRVKSAGPSTPVEITGLNEVPNAGDQFLVFKDEKTARS
VGEARATKQLEEQRSDKAKLSLDDLFEQIKQGDVKEINLVVKADVQGSVEALAAALQKIEVEGVRVKIIHTGVGAITESD
IILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIYKVIDEIEAAMKGMLDPEYEEKVIGMVEVRQTFKVSKIGTI
AGGYVTEGTITRDSGIRLIRDGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDMIEAYVMQEIERK
>Mature_715_residues
AKMRVYEYAKAINVSSKDIIAALKDMNIEVNNHMAMIEENAIKQLDQKFKKSAKPAGNKKDAGTGGQKPQQEAAKLNAGS
KPNKNRDGKKNNVQNTQFNNKNKKKNNNNKKNKRGKNHQSPQEKQFKPKKELPEKIEFTNSMSVGALAEELGKEPSEIIK
KLMLLGIMATINQDLDKDTVELIASEYGVEVEEVIVHEETEFEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIRK
TKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAADDGVMPQTVEAINHAKAAEVPI
IVAVNKIDKPTANPDRVMQELTEHGLVPEAWGGETIFVPLSALSGEGIDELIEMILLVSEVEELKANPNRRAKGTVIEAE
LDKGKGSVATLLVQNGTLHVGDPIVVGNTFGRVRAMVNDVGRRVKSAGPSTPVEITGLNEVPNAGDQFLVFKDEKTARSV
GEARATKQLEEQRSDKAKLSLDDLFEQIKQGDVKEINLVVKADVQGSVEALAAALQKIEVEGVRVKIIHTGVGAITESDI
ILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIYKVIDEIEAAMKGMLDPEYEEKVIGMVEVRQTFKVSKIGTIA
GGYVTEGTITRDSGIRLIRDGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDMIEAYVMQEIERK

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI53729339, Length=658, Percent_Identity=36.9300911854103, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI53729337, Length=658, Percent_Identity=36.9300911854103, Blast_Score=388, Evalue=1e-108,
Organism=Homo sapiens, GI84043963, Length=462, Percent_Identity=29.4372294372294, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI157426893, Length=262, Percent_Identity=28.6259541984733, Blast_Score=80, Evalue=9e-15,
Organism=Escherichia coli, GI1789559, Length=597, Percent_Identity=55.2763819095477, Blast_Score=633, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=229, Percent_Identity=33.6244541484716, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1788922, Length=234, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI48994988, Length=139, Percent_Identity=35.2517985611511, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790412, Length=125, Percent_Identity=37.6, Blast_Score=64, Evalue=5e-11,
Organism=Escherichia coli, GI1789737, Length=125, Percent_Identity=37.6, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994658, Length=549, Percent_Identity=36.247723132969, Blast_Score=321, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI212656558, Length=557, Percent_Identity=27.4685816876122, Blast_Score=136, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17557151, Length=220, Percent_Identity=29.5454545454545, Blast_Score=80, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=246, Percent_Identity=32.9268292682927, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25141371, Length=253, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324550, Length=621, Percent_Identity=38.1642512077295, Blast_Score=377, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6319282, Length=491, Percent_Identity=27.6985743380855, Blast_Score=144, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6324761, Length=239, Percent_Identity=31.7991631799163, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI28572034, Length=645, Percent_Identity=36.5891472868217, Blast_Score=374, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24656849, Length=500, Percent_Identity=29, Blast_Score=141, Evalue=2e-33,
Organism=Drosophila melanogaster, GI160714833, Length=118, Percent_Identity=55.0847457627119, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI78706572, Length=217, Percent_Identity=29.9539170506912, Blast_Score=84, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19922690, Length=229, Percent_Identity=29.2576419213974, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17137380, Length=264, Percent_Identity=28.7878787878788, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2_BACLD (Q65JI1)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_079061.1
- RefSeq:   YP_091476.1
- ProteinModelPortal:   Q65JI1
- SMR:   Q65JI1
- STRING:   Q65JI1
- EnsemblBacteria:   EBBACT00000058263
- EnsemblBacteria:   EBBACT00000060282
- GeneID:   3031085
- GeneID:   3097565
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01888
- KEGG:   bli:BL01224
- NMPDR:   fig|279010.5.peg.2592
- eggNOG:   COG0532
- GeneTree:   EBGT00070000032011
- HOGENOM:   HBG635083
- OMA:   RDNRGYS
- ProtClustDB:   PRK05306
- BioCyc:   BLIC279010-1:BLI01888-MONOMER
- BioCyc:   BLIC279010:BL01224-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00100_B
- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- TIGRFAMs:   TIGR00487
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 78579; Mature: 78447

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRVYEYAKAINVSSKDIIAALKDMNIEVNNHMAMIEENAIKQLDQKFKKSAKPAGNK
CCCCHHHHHHHHHCCCHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCC
KDAGTGGQKPQQEAAKLNAGSKPNKNRDGKKNNVQNTQFNNKNKKKNNNNKKNKRGKNHQ
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC
SPQEKQFKPKKELPEKIEFTNSMSVGALAEELGKEPSEIIKKLMLLGIMATINQDLDKDT
CCCHHCCCCHHHCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
VELIASEYGVEVEEVIVHEETEFEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIR
HHHHHHHHCCCHHHHEECCCHHHHHHCCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHH
KTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAAD
HHEEECCCCCCCHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEEEEEEECC
DGVMPQTVEAINHAKAAEVPIIVAVNKIDKPTANPDRVMQELTEHGLVPEAWGGETIFVP
CCCCHHHHHHHHCCHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEE
LSALSGEGIDELIEMILLVSEVEELKANPNRRAKGTVIEAELDKGKGSVATLLVQNGTLH
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEEECCEEE
VGDPIVVGNTFGRVRAMVNDVGRRVKSAGPSTPVEITGLNEVPNAGDQFLVFKDEKTARS
ECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCHHHHH
VGEARATKQLEEQRSDKAKLSLDDLFEQIKQGDVKEINLVVKADVQGSVEALAAALQKIE
HHHHHHHHHHHHHHCHHCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHE
VEGVRVKIIHTGVGAITESDIILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIY
ECCEEEEEEEECCCCEECCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
KVIDEIEAAMKGMLDPEYEEKVIGMVEVRQTFKVSKIGTIAGGYVTEGTITRDSGIRLIR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCEEECCEEECCCCCEEEE
DGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDMIEAYVMQEIERK
CCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
AKMRVYEYAKAINVSSKDIIAALKDMNIEVNNHMAMIEENAIKQLDQKFKKSAKPAGNK
CCCHHHHHHHHHCCCHHHHHHHHHHCCEEECCCEEEHHHHHHHHHHHHHHHHCCCCCCC
KDAGTGGQKPQQEAAKLNAGSKPNKNRDGKKNNVQNTQFNNKNKKKNNNNKKNKRGKNHQ
CCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCC
SPQEKQFKPKKELPEKIEFTNSMSVGALAEELGKEPSEIIKKLMLLGIMATINQDLDKDT
CCCHHCCCCHHHCCHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
VELIASEYGVEVEEVIVHEETEFEKYEEPDNEEDLEIRPPVVTIMGHVDHGKTTLLDSIR
HHHHHHHHCCCHHHHEECCCHHHHHHCCCCCCCCCEECCCEEEEEECCCCCHHHHHHHHH
KTKVVEGEAGGITQHIGAYQIEENGKKITFLDTPGHAAFTTMRARGAEVTDITILVVAAD
HHEEECCCCCCCHHHCCCEEEECCCCEEEEEECCCCHHHHHHHHCCCEEEEEEEEEEECC
DGVMPQTVEAINHAKAAEVPIIVAVNKIDKPTANPDRVMQELTEHGLVPEAWGGETIFVP
CCCCHHHHHHHHCCHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEE
LSALSGEGIDELIEMILLVSEVEELKANPNRRAKGTVIEAELDKGKGSVATLLVQNGTLH
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCEEEEEEECCEEE
VGDPIVVGNTFGRVRAMVNDVGRRVKSAGPSTPVEITGLNEVPNAGDQFLVFKDEKTARS
ECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCHHHHH
VGEARATKQLEEQRSDKAKLSLDDLFEQIKQGDVKEINLVVKADVQGSVEALAAALQKIE
HHHHHHHHHHHHHHCHHCCCCHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHHE
VEGVRVKIIHTGVGAITESDIILASASNAIVIGFNVRPDGNAKSTAEAENVDIRLHRIIY
ECCEEEEEEEECCCCEECCCEEEEECCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHH
KVIDEIEAAMKGMLDPEYEEKVIGMVEVRQTFKVSKIGTIAGGYVTEGTITRDSGIRLIR
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEECCCEEECCEEECCCCCEEEE
DGVVIFEGEVDVLKRFKDDVKEVSQGYECGITIKKYNDIREGDMIEAYVMQEIERK
CCEEEEECCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA