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The map label for this gene is rnhB

Identifier: 52785585

GI number: 52785585

Start: 1782661

End: 1783428

Strand: Direct

Name: rnhB

Synonym: BLi01826

Alternate gene names: 52785585

Gene position: 1782661-1783428 (Clockwise)

Preceding gene: 52785584

Following gene: 52785586

Centisome position: 42.22

GC content: 50.65

Gene sequence:

>768_bases
GTGAAAACATTAACCGTCAAAGAAATTAAAGAACATCTTCAATCGGTGAGCGATGAAAAGGACCCTTTTATAGAGCAGTG
CAAAAATGATGAGCGAAAAAGCGTTCAAGCGCTCGTCGACGCCTGGCTGAAGAAAAATGAAAGGCTTTCGGCCATGCGGG
AAGAGTGGCAGGCGATGACGTCTTTTGAACGGTCGCTGAGAGCGCGGGGCTATCAATATATCGCCGGAATCGACGAGGCG
GGCAGAGGGCCTCTCGCAGGGCCGGTTGTCGCCGCCGCTGTCATCCTCAAAGAAGATTGTGAAATATTAGGGCTGACAGA
TTCGAAAAAACTGTCAAAGCAAAAAAGAGAAGACTATTATTCATACATAATGGAAGAAGCCGCAGCAGTCGGCGTCGGGA
TCGCGGACGCCCATGAGATCGATGAGCTGAATATTTATGAAGCCTCCAAAGCGGCGATGCTGAAAGCGGTCCAAGCGCTG
GACGTCGCACCGGATTATCTTCTGATCGACGCCATGTCGCTTGCGGTCGATACAGAGCAGTCATCGATTATTAAAGGGGA
TGCCAAAAGCGCCTCGATAGCAGCAGGCGCATGCATCGCAAAGGTGACGAGGGACAGGCTGATGGATGAATACGCTGAAA
AGTATCCTCTCTACGGGTTTGAAAAGCATAAAGGCTACGGAACGAAGGAGCATCTGAATGCCCTGGCGAAATACGGCCCG
TCGCCGATACACCGAAGATCGTTCGCCCCTGTTAAAGCACATGAATAA

Upstream 100 bases:

>100_bases
TTGAAAAGCCGGAGGATATGTAAAAAGGCGCGCTTTTAAAAGAGCGGGCCTTTATTTTTGCAGAAGATGCGCCGATAAGA
ACATTAGGGGAGAAGAGAAA

Downstream 100 bases:

>100_bases
CGTTCCGGAGGAAGAATGACTTTGATGATGCTGAACGATGTACATAACAGCTTAAGAAAACAATTTGAACAAGCGGATTC
TCTCCAGCGGATCAGCGGAA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKTLTVKEIKEHLQSVSDEKDPFIEQCKNDERKSVQALVDAWLKKNERLSAMREEWQAMTSFERSLRARGYQYIAGIDEA
GRGPLAGPVVAAAVILKEDCEILGLTDSKKLSKQKREDYYSYIMEEAAAVGVGIADAHEIDELNIYEASKAAMLKAVQAL
DVAPDYLLIDAMSLAVDTEQSSIIKGDAKSASIAAGACIAKVTRDRLMDEYAEKYPLYGFEKHKGYGTKEHLNALAKYGP
SPIHRRSFAPVKAHE

Sequences:

>Translated_255_residues
MKTLTVKEIKEHLQSVSDEKDPFIEQCKNDERKSVQALVDAWLKKNERLSAMREEWQAMTSFERSLRARGYQYIAGIDEA
GRGPLAGPVVAAAVILKEDCEILGLTDSKKLSKQKREDYYSYIMEEAAAVGVGIADAHEIDELNIYEASKAAMLKAVQAL
DVAPDYLLIDAMSLAVDTEQSSIIKGDAKSASIAAGACIAKVTRDRLMDEYAEKYPLYGFEKHKGYGTKEHLNALAKYGP
SPIHRRSFAPVKAHE
>Mature_255_residues
MKTLTVKEIKEHLQSVSDEKDPFIEQCKNDERKSVQALVDAWLKKNERLSAMREEWQAMTSFERSLRARGYQYIAGIDEA
GRGPLAGPVVAAAVILKEDCEILGLTDSKKLSKQKREDYYSYIMEEAAAVGVGIADAHEIDELNIYEASKAAMLKAVQAL
DVAPDYLLIDAMSLAVDTEQSSIIKGDAKSASIAAGACIAKVTRDRLMDEYAEKYPLYGFEKHKGYGTKEHLNALAKYGP
SPIHRRSFAPVKAHE

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=182, Percent_Identity=48.3516483516484, Blast_Score=162, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_BACLD (Q65JP3)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078999.1
- RefSeq:   YP_091414.1
- ProteinModelPortal:   Q65JP3
- SMR:   Q65JP3
- STRING:   Q65JP3
- EnsemblBacteria:   EBBACT00000057621
- EnsemblBacteria:   EBBACT00000062582
- GeneID:   3030993
- GeneID:   3097777
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01826
- KEGG:   bli:BL01288
- NMPDR:   fig|279010.5.peg.2530
- eggNOG:   COG0164
- GeneTree:   EBGT00050000002677
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   BLIC279010-1:BLI01826-MONOMER
- BioCyc:   BLIC279010:BL01288-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 28302; Mature: 28302

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTVKEIKEHLQSVSDEKDPFIEQCKNDERKSVQALVDAWLKKNERLSAMREEWQAMT
CCCCCHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SFERSLRARGYQYIAGIDEAGRGPLAGPVVAAAVILKEDCEILGLTDSKKLSKQKREDYY
HHHHHHHHCCHHHEECCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCHHHHHHHHHHHH
SYIMEEAAAVGVGIADAHEIDELNIYEASKAAMLKAVQALDVAPDYLLIDAMSLAVDTEQ
HHHHHHHHHHCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCH
SSIIKGDAKSASIAAGACIAKVTRDRLMDEYAEKYPLYGFEKHKGYGTKEHLNALAKYGP
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCHHHHHHHHHCCC
SPIHRRSFAPVKAHE
CCHHHCCCCCCCCCC
>Mature Secondary Structure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CCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA