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The map label for this gene is trmD

Identifier: 52785582

GI number: 52785582

Start: 1780400

End: 1781131

Strand: Direct

Name: trmD

Synonym: BLi01823

Alternate gene names: 52785582

Gene position: 1780400-1781131 (Clockwise)

Preceding gene: 52785581

Following gene: 52785583

Centisome position: 42.16

GC content: 52.87

Gene sequence:

>732_bases
ATGAAAATCGATTTTTTAACGCTTTTTCCGGAAATGTTTGAAGGCGTGCTCGGCTCGTCGATCCTGCAAAAAGCTCAGGA
CAAGGACGCTGTCCGTTTTCGCGTGGTGAACTTTCGGGCCTATTCCGACAACAAGCATCAAACCGTTGACGATTATCCAT
ACGGCGGCGGAGCCGGAATGGTGCTGAAACCGCAGCCCGTCTTTGACGCGGTCGAAAAGCTGACGGCTGAAGCCGGAGGG
TCTCCGAGAATCATCCTCGTCTGCCCCCAGGGTGAACGCTATACCCAGCAGAAGGCGGAAGAACTGGCGCGGGAAGAGCA
TTTAATGTTCATTTGCGGCCATTATGAAGGGTATGATGAACGGATCAGGGAGCACCTGTCTACAGACGAGATTTCGATCG
GAGATTTCGTTTTGACAGGGGGAGAGCTGCCGGCGATGATGATCGCAGACAGCGTTGTGCGGCTTTTGCCGGGCGTGCTC
GGCAAGGAAGAATCGCACCTTGAAGACTCTTTCAGCACAGGGCTTCTTGAACACCCTCATTACACGCGGCCGGCAGATTA
TAAAGGCTTGAAGGTGCCGGACGTTCTGACATCCGGGAACCATGCGAAGATCAAAGAATGGCGGCGGAAGGAGTCTCTCA
GGCGCACATTTTTAAGGCGTCCCGACCTTCTGGAAAACTATCCTTTGTCAGAAGAAGAGCGAAAGTGGATTTCTGAATGG
AAAAACCGGTAG

Upstream 100 bases:

>100_bases
AGAAAGACGCGCTCATCCCTTATATTGACGCCGTTGTCAAAGAGATTAACGTAGCGGATAAAACGATCAAGATTCATATC
ATGGAAGGATTACTTGACGA

Downstream 100 bases:

>100_bases
ATTCTATTGCGCCCGGGAAAAAGGTGTGGTATGATACTACTTGTGACTTGAACGGGAACGCTCGTTTAAGTTTGAAAACG
ATGTTCCGCTGTGCCGGTTA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKIDFLTLFPEMFEGVLGSSILQKAQDKDAVRFRVVNFRAYSDNKHQTVDDYPYGGGAGMVLKPQPVFDAVEKLTAEAGG
SPRIILVCPQGERYTQQKAEELAREEHLMFICGHYEGYDERIREHLSTDEISIGDFVLTGGELPAMMIADSVVRLLPGVL
GKEESHLEDSFSTGLLEHPHYTRPADYKGLKVPDVLTSGNHAKIKEWRRKESLRRTFLRRPDLLENYPLSEEERKWISEW
KNR

Sequences:

>Translated_243_residues
MKIDFLTLFPEMFEGVLGSSILQKAQDKDAVRFRVVNFRAYSDNKHQTVDDYPYGGGAGMVLKPQPVFDAVEKLTAEAGG
SPRIILVCPQGERYTQQKAEELAREEHLMFICGHYEGYDERIREHLSTDEISIGDFVLTGGELPAMMIADSVVRLLPGVL
GKEESHLEDSFSTGLLEHPHYTRPADYKGLKVPDVLTSGNHAKIKEWRRKESLRRTFLRRPDLLENYPLSEEERKWISEW
KNR
>Mature_243_residues
MKIDFLTLFPEMFEGVLGSSILQKAQDKDAVRFRVVNFRAYSDNKHQTVDDYPYGGGAGMVLKPQPVFDAVEKLTAEAGG
SPRIILVCPQGERYTQQKAEELAREEHLMFICGHYEGYDERIREHLSTDEISIGDFVLTGGELPAMMIADSVVRLLPGVL
GKEESHLEDSFSTGLLEHPHYTRPADYKGLKVPDVLTSGNHAKIKEWRRKESLRRTFLRRPDLLENYPLSEEERKWISEW
KNR

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=242, Percent_Identity=47.5206611570248, Blast_Score=227, Evalue=6e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BACLD (Q65JP6)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078996.2
- RefSeq:   YP_091411.1
- ProteinModelPortal:   Q65JP6
- SMR:   Q65JP6
- STRING:   Q65JP6
- EnsemblBacteria:   EBBACT00000055885
- EnsemblBacteria:   EBBACT00000061156
- GeneID:   3030991
- GeneID:   3097986
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01823
- KEGG:   bli:BL01291
- NMPDR:   fig|279010.5.peg.2527
- eggNOG:   COG0336
- GeneTree:   EBGT00050000001149
- HOGENOM:   HBG285805
- ProtClustDB:   PRK00026
- BioCyc:   BLIC279010-1:BLI01823-MONOMER
- BioCyc:   BLIC279010:BL01291-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27825; Mature: 27825

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: BINDING 113-113

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDFLTLFPEMFEGVLGSSILQKAQDKDAVRFRVVNFRAYSDNKHQTVDDYPYGGGAGM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
VLKPQPVFDAVEKLTAEAGGSPRIILVCPQGERYTQQKAEELAREEHLMFICGHYEGYDE
EECCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
RIREHLSTDEISIGDFVLTGGELPAMMIADSVVRLLPGVLGKEESHLEDSFSTGLLEHPH
HHHHHCCCCCEECCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
YTRPADYKGLKVPDVLTSGNHAKIKEWRRKESLRRTFLRRPDLLENYPLSEEERKWISEW
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHH
KNR
CCC
>Mature Secondary Structure
MKIDFLTLFPEMFEGVLGSSILQKAQDKDAVRFRVVNFRAYSDNKHQTVDDYPYGGGAGM
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
VLKPQPVFDAVEKLTAEAGGSPRIILVCPQGERYTQQKAEELAREEHLMFICGHYEGYDE
EECCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEECCCCHHH
RIREHLSTDEISIGDFVLTGGELPAMMIADSVVRLLPGVLGKEESHLEDSFSTGLLEHPH
HHHHHCCCCCEECCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCC
YTRPADYKGLKVPDVLTSGNHAKIKEWRRKESLRRTFLRRPDLLENYPLSEEERKWISEW
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHH
KNR
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA