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The map label for this gene is murB

Identifier: 52785499

GI number: 52785499

Start: 1696376

End: 1697287

Strand: Direct

Name: murB

Synonym: BLi01740

Alternate gene names: 52785499

Gene position: 1696376-1697287 (Clockwise)

Preceding gene: 52785498

Following gene: 52785500

Centisome position: 40.17

GC content: 50.77

Gene sequence:

>912_bases
ATGGATAAGGTGATACAAGAATTAAAAGAACTTGAAGTCGGCAAAGTTCTGGAAAACGAGCCGCTCAGCAACCATACAAC
GATTAAAATCGGAGGACCGGCTGACGTGCTGGTGATTCCGAAAGACATTCAAGCTGTCAAAGATACGATGAAGGTTGTCA
AGAAGCACGGCGTCAAGTGGACGGCCATCGGCAGAGGCTCGAATCTTCTCGTTCTCGATGAAGGGATAAGAGGCGTCGTC
ATCAAGCTCGGCCAGGGACTCGACCATATGGAAATCGACGGCGAGCAAGTGACGGTCGGCGGCGGCTACTCTGTCGTAAG
GCTTGCGACAGGCATCAGCAAAAAAGGGCTTTCCGGCCTTGAATTTGCCGCAGGCATTCCTGGTTCGGTCGGCGGAGCGG
TTTACATGAATGCCGGCGCCCACGGCTCAGATATCAGCAAAGTATTGGTGAAAGCATTGATCCTTTTTGAAGACGGAACG
ATCGAATGGCTGACGAATGAAGAGATGGCGTTCAGCTACCGCACATCCATCCTTCAAAACAAGCGGCCGGGCATTTGTCT
TGAAGCCGTTCTCCAATTGGAGCAAAAAGAACGCGATCAGATCGTCGCGCAGATGCAGAAAAACAAAGACTACCGAAAAG
AAACGCAGCCTGTGTCCAACCCTTGCGCAGGCAGCATCTTCAGAAACCCGCTGCCTGAACATGCGGGCCGGCTAGTTGAA
GAGGCTGGTTTGAAAGGCCATCAGATCGGCGGGGCAAAGGTTTCGGAGATGCACGGCAATTTCATTGTCAACGCAGGCGG
CGCAACAGCGAAAGACGTCCTTGATTTGATTGCATTCATTCAGAAAACGATTAAGGAAAAATACGATATCGACATGCACA
CTGAGGTCGAAATTATCGGAGAAAAACGGTAA

Upstream 100 bases:

>100_bases
TCGCTTGGCGTGCCGGACGCTGCGGAACGCTTGTACAATGTGCTGAAAGAATTGAAACATCATGCAAAATGATAAGACAG
AAGGATTGTGGAGGTTTTCG

Downstream 100 bases:

>100_bases
ACGGCCTCTCTACTTCAGATCGCCTTATGATATAATGTATGAAACAAACAAGCAAACGGCATCCTAGTATGTCGTTTGTT
TCGCTAAACGGAAGCTTTTT

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDKVIQELKELEVGKVLENEPLSNHTTIKIGGPADVLVIPKDIQAVKDTMKVVKKHGVKWTAIGRGSNLLVLDEGIRGVV
IKLGQGLDHMEIDGEQVTVGGGYSVVRLATGISKKGLSGLEFAAGIPGSVGGAVYMNAGAHGSDISKVLVKALILFEDGT
IEWLTNEEMAFSYRTSILQNKRPGICLEAVLQLEQKERDQIVAQMQKNKDYRKETQPVSNPCAGSIFRNPLPEHAGRLVE
EAGLKGHQIGGAKVSEMHGNFIVNAGGATAKDVLDLIAFIQKTIKEKYDIDMHTEVEIIGEKR

Sequences:

>Translated_303_residues
MDKVIQELKELEVGKVLENEPLSNHTTIKIGGPADVLVIPKDIQAVKDTMKVVKKHGVKWTAIGRGSNLLVLDEGIRGVV
IKLGQGLDHMEIDGEQVTVGGGYSVVRLATGISKKGLSGLEFAAGIPGSVGGAVYMNAGAHGSDISKVLVKALILFEDGT
IEWLTNEEMAFSYRTSILQNKRPGICLEAVLQLEQKERDQIVAQMQKNKDYRKETQPVSNPCAGSIFRNPLPEHAGRLVE
EAGLKGHQIGGAKVSEMHGNFIVNAGGATAKDVLDLIAFIQKTIKEKYDIDMHTEVEIIGEKR
>Mature_303_residues
MDKVIQELKELEVGKVLENEPLSNHTTIKIGGPADVLVIPKDIQAVKDTMKVVKKHGVKWTAIGRGSNLLVLDEGIRGVV
IKLGQGLDHMEIDGEQVTVGGGYSVVRLATGISKKGLSGLEFAAGIPGSVGGAVYMNAGAHGSDISKVLVKALILFEDGT
IEWLTNEEMAFSYRTSILQNKRPGICLEAVLQLEQKERDQIVAQMQKNKDYRKETQPVSNPCAGSIFRNPLPEHAGRLVE
EAGLKGHQIGGAKVSEMHGNFIVNAGGATAKDVLDLIAFIQKTIKEKYDIDMHTEVEIIGEKR

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=329, Percent_Identity=26.7477203647416, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_BACLD (Q65JX9)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078916.1
- RefSeq:   YP_091328.1
- ProteinModelPortal:   Q65JX9
- SMR:   Q65JX9
- STRING:   Q65JX9
- EnsemblBacteria:   EBBACT00000055302
- EnsemblBacteria:   EBBACT00000062201
- GeneID:   3030889
- GeneID:   3097817
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01740
- KEGG:   bli:BL02244
- NMPDR:   fig|279010.5.peg.1587
- eggNOG:   COG0812
- GeneTree:   EBGT00050000001391
- HOGENOM:   HBG686573
- OMA:   ILIVPKH
- ProtClustDB:   PRK13905
- BioCyc:   BLIC279010-1:BLI01740-MONOMER
- BioCyc:   BLIC279010:BL02244-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 32810; Mature: 32810

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 174-174 ACT_SITE 225-225 ACT_SITE 295-295

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKVIQELKELEVGKVLENEPLSNHTTIKIGGPADVLVIPKDIQAVKDTMKVVKKHGVKW
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHCCCEE
TAIGRGSNLLVLDEGIRGVVIKLGQGLDHMEIDGEQVTVGGGYSVVRLATGISKKGLSGL
EEECCCCEEEEECCCCCEEEEEECCCCCCEEECCCEEEECCCCEEEHHHHCCCCCCCCCC
EFAAGIPGSVGGAVYMNAGAHGSDISKVLVKALILFEDGTIEWLTNEEMAFSYRTSILQN
HHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCC
KRPGICLEAVLQLEQKERDQIVAQMQKNKDYRKETQPVSNPCAGSIFRNPLPEHAGRLVE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHH
EAGLKGHQIGGAKVSEMHGNFIVNAGGATAKDVLDLIAFIQKTIKEKYDIDMHTEVEIIG
HCCCCCCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
EKR
CCC
>Mature Secondary Structure
MDKVIQELKELEVGKVLENEPLSNHTTIKIGGPADVLVIPKDIQAVKDTMKVVKKHGVKW
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHCCCEE
TAIGRGSNLLVLDEGIRGVVIKLGQGLDHMEIDGEQVTVGGGYSVVRLATGISKKGLSGL
EEECCCCEEEEECCCCCEEEEEECCCCCCEEECCCEEEECCCCEEEHHHHCCCCCCCCCC
EFAAGIPGSVGGAVYMNAGAHGSDISKVLVKALILFEDGTIEWLTNEEMAFSYRTSILQN
HHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCC
KRPGICLEAVLQLEQKERDQIVAQMQKNKDYRKETQPVSNPCAGSIFRNPLPEHAGRLVE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHH
EAGLKGHQIGGAKVSEMHGNFIVNAGGATAKDVLDLIAFIQKTIKEKYDIDMHTEVEIIG
HCCCCCCCCCCCEEEECCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEE
EKR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA