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The map label for this gene is pbpB [H]

Identifier: 52785492

GI number: 52785492

Start: 1685690

End: 1687825

Strand: Direct

Name: pbpB [H]

Synonym: BLi01733

Alternate gene names: 52785492

Gene position: 1685690-1687825 (Clockwise)

Preceding gene: 52785491

Following gene: 52785493

Centisome position: 39.92

GC content: 48.88

Gene sequence:

>2136_bases
ATGCCGAAAAAAAATAAATTCATGAACAGAGGAGCGGCAATACTCAGCATTTGTTTTGCCCTCTTTTTCTTTGTCATCGT
AGGACGGTTTGCGTTTATTCAAGCGACGGGAAAAGTGAACGGCGAAGTGCTTGCGCAGAAGGCGAACGAACAGTATGAGA
AAAAGCGGACGATCGAAGCCCACCGAGGATCGATCCTTGACCGGAACGGAAAAGTGCTTGCCGAAGATGCTACGACATAT
AAGCTTGTCGCGATTATGAGCGAAAAGATGAAGCCTCAATACGTTAAGGACAAGCAGCTGACAGCTGAGAAGCTCGCGCC
GATCCTCGGCATGGATGAGGGGAAAGTGCTCGATATTCTAAATAAAAAAGGAGCGTTTCAGGTCGAATTCGGATCGGCGG
GACGGGATTTGACATACGCCCAAAAAGAGAAAATCGAAAAGCTGAAGCTGCCGGGCATCGTGTTCACCCGCGATACGAAG
CGCTACTATCCAAACGGAATCTTCGCCTCCAACCTCATCGGCTATTCGCAGATCAACGAAAAAACGGGTGAAATGCAAGG
CATGATGGGCCTTGAAAAAACGCTGAACGATTATTTGAAAGAAACGGACGGATACATGACGTACGATACCGACAGAACGG
GCTGGCGCCTGCCGAACAGCAATGAAAAGATTAAAGCGCCAAACAACGGAGACAACGTGTATTTGACGATCGATCAAAAA
ATCCAGGCGTTTTTGGAAGACAGCATGACGACCGTTCAAAAAGAGTACAAGCCGAAAAAGATTATGGCGGCGGTTGTTGA
TCCGAAAACGGGCAAAGTGCTTGCGATGGGGCAAAGGCCGAGCTTCAACCCGAACGTGCGCGATGTCACGAACTATTACA
ACGACCTTGTCTCCTACAGCTTTGAGCCGGGCTCGACGATGAAGATTTTCACATTGGCGGCGGCCATCCAAGAAGGCGTA
TATAATGGAGGAGAACAATACAAGTCTGGATCATATTCGGTTTCTAAAAAAGATAAGCCGATCAAAGACCATAACGGTGG
AAACGGCTGGGGCCCGATCACTTTTAATGAAGGTGTTGAACGCTCCTCAAACGTCGCGTTTGCGATCCTTGCCAACGATA
AACTCGGCACGGACCGGTTCAATCAATATTTGCGGAAATTCCATTTTTACGACAAAACGGGGATTGACCTGCCTGGGGAA
GCGAACAGCAAAATCAACTTTGAATATTTGCGGGATAAAGTGTCGACGGCTTTTGGACAGAGCTCTGCTGTCACGCCGAT
CCAGCAGCTTCAGGCCGCGACGGCGATCGCCAATAACGGAAAAATGATGAAGCCTTATGTGATCGATCATGTCGTTGATT
CTGACAAAAACAAAACGATTTATCAAAATAAACCGGAACAAGTCGGCCAGCCGATCTCTGCCAAAACGGCAAAAGAAGTG
CGCGAGCTGTTGGGAAAAGTCGTCACATCTGAAAAAGGAACGGGGCAGCCGTACAAGATCGAAGGATTTGATGTCGCCGG
AAAAACCGGTACGGCGCAAATTGCCGGAGAAGACGGCAGATATTTGACCGGCCGCGAAAACTACGTGTTCTCCTTCATGG
GCATGGCCCCGAAGGACGATCCGCAGCTTCTCGTTTATGTGGCCGTGCAGCAGCCGCAATTAAAAGCTACGGAAACAGGC
TCTGCTCCGGTGTCAAAAATTTTCAATCCGGTGATGAAAAACAGTCTTCACTACTTAAATATCACACCGAAAGAGACTGA
AAAAGAAGAAAAGCAGGAAGAAAAACAGCTGAAAATGCCTGATTTCTCCGGCAAACGAGCCGAATCCGCCGCAAAAGAGG
CGCAAGGCAGCAGCCTTGTTCCCGTTACGATCGGCGATGGCTTGGCTGTGAAAGAACAGTACCCGAAAGCGGGCGAAGCC
GTCCTGCCGAACCAACGGGTCTTCCTGAAAACCGACGGGAAAATCACACTCCCAGATATGACAGGCTGGTCAAAACGGGA
CGCCCTCCGCTTCGGCGCCATGGCAGGCATTCATATCGAAACGAGCGGCCAGGGCTATGTGAAGAGTCAAAATGTAAAAG
CAGGCAAAGAATTAAAAGCAAATTCAGTCGTGAAAGTCAGATTGAAAAACGAATAG

Upstream 100 bases:

>100_bases
CGATATGCTGAAAGAGCCGGACCGTATCATGGACATCGCCCGGAAAAAAGGCCTTAAATTGGGTGATAAAAAAGTGAAAG
ACATACAGGAATGATGCAAA

Downstream 100 bases:

>100_bases
ATCCCGTCAAAGGCTATCGGCTCAAAGTCGATAGCCTTCCGTTTGGACGCGGCCGCCTTGAATAGATGAAAAATCGGGCC
GTTCTATCAAAACGCTCCGA

Product: PbpB

Products: NA

Alternate protein names: PBP-2B; Penicillin-binding protein B [H]

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MPKKNKFMNRGAAILSICFALFFFVIVGRFAFIQATGKVNGEVLAQKANEQYEKKRTIEAHRGSILDRNGKVLAEDATTY
KLVAIMSEKMKPQYVKDKQLTAEKLAPILGMDEGKVLDILNKKGAFQVEFGSAGRDLTYAQKEKIEKLKLPGIVFTRDTK
RYYPNGIFASNLIGYSQINEKTGEMQGMMGLEKTLNDYLKETDGYMTYDTDRTGWRLPNSNEKIKAPNNGDNVYLTIDQK
IQAFLEDSMTTVQKEYKPKKIMAAVVDPKTGKVLAMGQRPSFNPNVRDVTNYYNDLVSYSFEPGSTMKIFTLAAAIQEGV
YNGGEQYKSGSYSVSKKDKPIKDHNGGNGWGPITFNEGVERSSNVAFAILANDKLGTDRFNQYLRKFHFYDKTGIDLPGE
ANSKINFEYLRDKVSTAFGQSSAVTPIQQLQAATAIANNGKMMKPYVIDHVVDSDKNKTIYQNKPEQVGQPISAKTAKEV
RELLGKVVTSEKGTGQPYKIEGFDVAGKTGTAQIAGEDGRYLTGRENYVFSFMGMAPKDDPQLLVYVAVQQPQLKATETG
SAPVSKIFNPVMKNSLHYLNITPKETEKEEKQEEKQLKMPDFSGKRAESAAKEAQGSSLVPVTIGDGLAVKEQYPKAGEA
VLPNQRVFLKTDGKITLPDMTGWSKRDALRFGAMAGIHIETSGQGYVKSQNVKAGKELKANSVVKVRLKNE

Sequences:

>Translated_711_residues
MPKKNKFMNRGAAILSICFALFFFVIVGRFAFIQATGKVNGEVLAQKANEQYEKKRTIEAHRGSILDRNGKVLAEDATTY
KLVAIMSEKMKPQYVKDKQLTAEKLAPILGMDEGKVLDILNKKGAFQVEFGSAGRDLTYAQKEKIEKLKLPGIVFTRDTK
RYYPNGIFASNLIGYSQINEKTGEMQGMMGLEKTLNDYLKETDGYMTYDTDRTGWRLPNSNEKIKAPNNGDNVYLTIDQK
IQAFLEDSMTTVQKEYKPKKIMAAVVDPKTGKVLAMGQRPSFNPNVRDVTNYYNDLVSYSFEPGSTMKIFTLAAAIQEGV
YNGGEQYKSGSYSVSKKDKPIKDHNGGNGWGPITFNEGVERSSNVAFAILANDKLGTDRFNQYLRKFHFYDKTGIDLPGE
ANSKINFEYLRDKVSTAFGQSSAVTPIQQLQAATAIANNGKMMKPYVIDHVVDSDKNKTIYQNKPEQVGQPISAKTAKEV
RELLGKVVTSEKGTGQPYKIEGFDVAGKTGTAQIAGEDGRYLTGRENYVFSFMGMAPKDDPQLLVYVAVQQPQLKATETG
SAPVSKIFNPVMKNSLHYLNITPKETEKEEKQEEKQLKMPDFSGKRAESAAKEAQGSSLVPVTIGDGLAVKEQYPKAGEA
VLPNQRVFLKTDGKITLPDMTGWSKRDALRFGAMAGIHIETSGQGYVKSQNVKAGKELKANSVVKVRLKNE
>Mature_710_residues
PKKNKFMNRGAAILSICFALFFFVIVGRFAFIQATGKVNGEVLAQKANEQYEKKRTIEAHRGSILDRNGKVLAEDATTYK
LVAIMSEKMKPQYVKDKQLTAEKLAPILGMDEGKVLDILNKKGAFQVEFGSAGRDLTYAQKEKIEKLKLPGIVFTRDTKR
YYPNGIFASNLIGYSQINEKTGEMQGMMGLEKTLNDYLKETDGYMTYDTDRTGWRLPNSNEKIKAPNNGDNVYLTIDQKI
QAFLEDSMTTVQKEYKPKKIMAAVVDPKTGKVLAMGQRPSFNPNVRDVTNYYNDLVSYSFEPGSTMKIFTLAAAIQEGVY
NGGEQYKSGSYSVSKKDKPIKDHNGGNGWGPITFNEGVERSSNVAFAILANDKLGTDRFNQYLRKFHFYDKTGIDLPGEA
NSKINFEYLRDKVSTAFGQSSAVTPIQQLQAATAIANNGKMMKPYVIDHVVDSDKNKTIYQNKPEQVGQPISAKTAKEVR
ELLGKVVTSEKGTGQPYKIEGFDVAGKTGTAQIAGEDGRYLTGRENYVFSFMGMAPKDDPQLLVYVAVQQPQLKATETGS
APVSKIFNPVMKNSLHYLNITPKETEKEEKQEEKQLKMPDFSGKRAESAAKEAQGSSLVPVTIGDGLAVKEQYPKAGEAV
LPNQRVFLKTDGKITLPDMTGWSKRDALRFGAMAGIHIETSGQGYVKSQNVKAGKELKANSVVKVRLKNE

Specific function: Penicillin-binding proteins (PBPs) function in the late steps of murein biosynthesis. PBP-2B is required for vegetative cell division and sporulation septation. Beta-lactamase inactivates the PBPs by acylating an essential serine residue in the active sit

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PASTA domains [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=603, Percent_Identity=26.865671641791, Blast_Score=166, Evalue=7e-42,
Organism=Escherichia coli, GI1786854, Length=563, Percent_Identity=25.044404973357, Blast_Score=131, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005543
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03793 PASTA; PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 78845; Mature: 78714

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKKNKFMNRGAAILSICFALFFFVIVGRFAFIQATGKVNGEVLAQKANEQYEKKRTIEA
CCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHH
HRGSILDRNGKVLAEDATTYKLVAIMSEKMKPQYVKDKQLTAEKLAPILGMDEGKVLDIL
HCCCEECCCCCEEECCCCCEEHHHHHHHHCCCCHHCCHHHHHHHHHHHHCCCCCCEEEEC
NKKGAFQVEFGSAGRDLTYAQKEKIEKLKLPGIVFTRDTKRYYPNGIFASNLIGYSQINE
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHCCCCCHHHHCCCHHHHHH
KTGEMQGMMGLEKTLNDYLKETDGYMTYDTDRTGWRLPNSNEKIKAPNNGDNVYLTIDQK
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECCCCCCEEEEEEHHH
IQAFLEDSMTTVQKEYKPKKIMAAVVDPKTGKVLAMGQRPSFNPNVRDVTNYYNDLVSYS
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHEEC
FEPGSTMKIFTLAAAIQEGVYNGGEQYKSGSYSVSKKDKPIKDHNGGNGWGPITFNEGVE
CCCCCCEEHHHHHHHHHHHHCCCCHHCCCCCEEECCCCCCCCCCCCCCCCCCCEECCCCC
RSSNVAFAILANDKLGTDRFNQYLRKFHFYDKTGIDLPGEANSKINFEYLRDKVSTAFGQ
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCC
SSAVTPIQQLQAATAIANNGKMMKPYVIDHVVDSDKNKTIYQNKPEQVGQPISAKTAKEV
CCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHH
RELLGKVVTSEKGTGQPYKIEGFDVAGKTGTAQIAGEDGRYLTGRENYVFSFMGMAPKDD
HHHHHHHHCCCCCCCCCEEEECEEECCCCCCEEEECCCCCEEECCCCEEEEEECCCCCCC
PQLLVYVAVQQPQLKATETGSAPVSKIFNPVMKNSLHYLNITPKETEKEEKQEEKQLKMP
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCC
DFSGKRAESAAKEAQGSSLVPVTIGDGLAVKEQYPKAGEAVLPNQRVFLKTDGKITLPDM
CCCCCHHHHHHHHCCCCEEEEEEECCCEEEHHCCCCCCCEECCCCEEEEEECCEEECCCC
TGWSKRDALRFGAMAGIHIETSGQGYVKSQNVKAGKELKANSVVKVRLKNE
CCCCHHHHHHHCCEEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
PKKNKFMNRGAAILSICFALFFFVIVGRFAFIQATGKVNGEVLAQKANEQYEKKRTIEA
CCCHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHH
HRGSILDRNGKVLAEDATTYKLVAIMSEKMKPQYVKDKQLTAEKLAPILGMDEGKVLDIL
HCCCEECCCCCEEECCCCCEEHHHHHHHHCCCCHHCCHHHHHHHHHHHHCCCCCCEEEEC
NKKGAFQVEFGSAGRDLTYAQKEKIEKLKLPGIVFTRDTKRYYPNGIFASNLIGYSQINE
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEECCCHHHCCCCCHHHHCCCHHHHHH
KTGEMQGMMGLEKTLNDYLKETDGYMTYDTDRTGWRLPNSNEKIKAPNNGDNVYLTIDQK
HHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECCCCCCEEEEEEHHH
IQAFLEDSMTTVQKEYKPKKIMAAVVDPKTGKVLAMGQRPSFNPNVRDVTNYYNDLVSYS
HHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHEEC
FEPGSTMKIFTLAAAIQEGVYNGGEQYKSGSYSVSKKDKPIKDHNGGNGWGPITFNEGVE
CCCCCCEEHHHHHHHHHHHHCCCCHHCCCCCEEECCCCCCCCCCCCCCCCCCCEECCCCC
RSSNVAFAILANDKLGTDRFNQYLRKFHFYDKTGIDLPGEANSKINFEYLRDKVSTAFGQ
CCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCC
SSAVTPIQQLQAATAIANNGKMMKPYVIDHVVDSDKNKTIYQNKPEQVGQPISAKTAKEV
CCCCCHHHHHHHHHHHHCCCCEECCHHHHHHHCCCCCCCCCCCCHHHHCCCCCHHHHHHH
RELLGKVVTSEKGTGQPYKIEGFDVAGKTGTAQIAGEDGRYLTGRENYVFSFMGMAPKDD
HHHHHHHHCCCCCCCCCEEEECEEECCCCCCEEEECCCCCEEECCCCEEEEEECCCCCCC
PQLLVYVAVQQPQLKATETGSAPVSKIFNPVMKNSLHYLNITPKETEKEEKQEEKQLKMP
CCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHCCCC
DFSGKRAESAAKEAQGSSLVPVTIGDGLAVKEQYPKAGEAVLPNQRVFLKTDGKITLPDM
CCCCCHHHHHHHHCCCCEEEEEEECCCEEEHHCCCCCCCEECCCCEEEEEECCEEECCCC
TGWSKRDALRFGAMAGIHIETSGQGYVKSQNVKAGKELKANSVVKVRLKNE
CCCCHHHHHHHCCEEEEEEEECCCCEEECCCCCCCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8244929; 9384377; 8289242 [H]