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The map label for this gene is mraW

Identifier: 52785490

GI number: 52785490

Start: 1684360

End: 1685298

Strand: Direct

Name: mraW

Synonym: BLi01731

Alternate gene names: 52785490

Gene position: 1684360-1685298 (Clockwise)

Preceding gene: 52785489

Following gene: 52785491

Centisome position: 39.89

GC content: 46.96

Gene sequence:

>939_bases
ATGTTTGAGCATACAACAGTATTATTAAAAGAAACAGTTGATGGTTTAAATGTAAAAGAAGACGGTACATATGTAGACTG
CACATTAGGCGGTGCCGGTCACAGCGAGTATTTGCTTTCACAGCTGTCTGAAAAAGGCAAATTGGTCGCTTTTGACCAGG
ATGAAACCGCACTCCGCCATGCGAAGGAAAAACTGGCGGATTATGAAGGACAAGTCGTTTTCATAAAAAGCAATTTTCGG
TATTTAAAAGACCGTCTAAACGAAATCGGCATCACGAAAGTGGACGGTGTTTTATTTGATTTAGGCGTCTCATCTCCTCA
GCTGGATACACCCGAAAGGGGCTTCAGCTATCATCATGATGCACCTCTTGATATGCGAATGGACCAGTCGGCGGGCCTTT
CGGCAAAGGAAGTCGTCAATGAATGGCCTTATGAGGATCTCGTGAAAATCTTCTTTAAATACGGAGAAGAGAAGTTCAGC
AAGCAAATTGCCAGGAAAATCGAAAAAGCAAGAGAACAATCGCCTATTGAAACAACAGGCCAGCTTGTCGATATCATCAA
AGAGGCGATTCCCGCACCGGCAAGAAGAAGCGGCGGGCATCCGGCTAAACGGATCTTCCAGGCGATTCGAATTGCGGTAA
ACGATGAGTTGAGGGTATTTGAAGAAGCGCTTTTGCAGGCGATTGAGGTGCTGAAGCCGGGCGGAAGAGTATCGGTCATC
ACATTCCACTCGCTGGAAGACCGGATCTGCAAATCGACATTTAAAGACATGTCGTCGCCTCCTGAACTGCCTCACGGACT
GCCCGTGATCCCCAAGGAGTTTGAACCGAAATTAAAATTAGTCAACAGAAAGCCGATCACAGCTTCCGAAGAGGAGCTTG
AACACAATAACCGTGCGCGCTCAGCGAAGCTGCGCATCGCGGAAAAAGCACGAGAGTGA

Upstream 100 bases:

>100_bases
AAATAGCTGAAAACATGATCGGATTTGATATATAATGAATCTTTGCGCATGCGGCGCTTCTGCAATCAGTTCATTTGAAA
AAGGTGGGACTAGTAAAGAA

Downstream 100 bases:

>100_bases
GCTGTACGAATTAAAAGGAGGTCATCAGTCTATGAGCAATTTGGCTTATAAAGTAGAAAAACAGCAAAGACAGACAGACA
GTGCAGAACAATCCGTTATC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MFEHTTVLLKETVDGLNVKEDGTYVDCTLGGAGHSEYLLSQLSEKGKLVAFDQDETALRHAKEKLADYEGQVVFIKSNFR
YLKDRLNEIGITKVDGVLFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSAGLSAKEVVNEWPYEDLVKIFFKYGEEKFS
KQIARKIEKAREQSPIETTGQLVDIIKEAIPAPARRSGGHPAKRIFQAIRIAVNDELRVFEEALLQAIEVLKPGGRVSVI
TFHSLEDRICKSTFKDMSSPPELPHGLPVIPKEFEPKLKLVNRKPITASEEELEHNNRARSAKLRIAEKARE

Sequences:

>Translated_312_residues
MFEHTTVLLKETVDGLNVKEDGTYVDCTLGGAGHSEYLLSQLSEKGKLVAFDQDETALRHAKEKLADYEGQVVFIKSNFR
YLKDRLNEIGITKVDGVLFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSAGLSAKEVVNEWPYEDLVKIFFKYGEEKFS
KQIARKIEKAREQSPIETTGQLVDIIKEAIPAPARRSGGHPAKRIFQAIRIAVNDELRVFEEALLQAIEVLKPGGRVSVI
TFHSLEDRICKSTFKDMSSPPELPHGLPVIPKEFEPKLKLVNRKPITASEEELEHNNRARSAKLRIAEKARE
>Mature_312_residues
MFEHTTVLLKETVDGLNVKEDGTYVDCTLGGAGHSEYLLSQLSEKGKLVAFDQDETALRHAKEKLADYEGQVVFIKSNFR
YLKDRLNEIGITKVDGVLFDLGVSSPQLDTPERGFSYHHDAPLDMRMDQSAGLSAKEVVNEWPYEDLVKIFFKYGEEKFS
KQIARKIEKAREQSPIETTGQLVDIIKEAIPAPARRSGGHPAKRIFQAIRIAVNDELRVFEEALLQAIEVLKPGGRVSVI
TFHSLEDRICKSTFKDMSSPPELPHGLPVIPKEFEPKLKLVNRKPITASEEELEHNNRARSAKLRIAEKARE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=35.6083086053413, Blast_Score=166, Evalue=2e-41,
Organism=Homo sapiens, GI165377202, Length=190, Percent_Identity=39.4736842105263, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1786270, Length=315, Percent_Identity=45.7142857142857, Blast_Score=251, Evalue=4e-68,
Organism=Drosophila melanogaster, GI62472493, Length=329, Percent_Identity=38.6018237082067, Blast_Score=175, Evalue=3e-44,
Organism=Drosophila melanogaster, GI28571637, Length=329, Percent_Identity=38.6018237082067, Blast_Score=175, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_BACLD (Q65JY8)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078907.1
- RefSeq:   YP_091319.1
- ProteinModelPortal:   Q65JY8
- STRING:   Q65JY8
- EnsemblBacteria:   EBBACT00000057047
- EnsemblBacteria:   EBBACT00000062274
- GeneID:   3030880
- GeneID:   3100617
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01731
- KEGG:   bli:BL02234
- NMPDR:   fig|279010.5.peg.1578
- eggNOG:   COG0275
- GeneTree:   EBGT00050000002010
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- BioCyc:   BLIC279010-1:BLI01731-MONOMER
- BioCyc:   BLIC279010:BL02234-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 35196; Mature: 35196

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEHTTVLLKETVDGLNVKEDGTYVDCTLGGAGHSEYLLSQLSEKGKLVAFDQDETALRH
CCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHHH
AKEKLADYEGQVVFIKSNFRYLKDRLNEIGITKVDGVLFDLGVSSPQLDTPERGFSYHHD
HHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQSAGLSAKEVVNEWPYEDLVKIFFKYGEEKFSKQIARKIEKAREQSPIETTG
CCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QLVDIIKEAIPAPARRSGGHPAKRIFQAIRIAVNDELRVFEEALLQAIEVLKPGGRVSVI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKSTFKDMSSPPELPHGLPVIPKEFEPKLKLVNRKPITASEEELEHNNRAR
EEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHCCHHH
SAKLRIAEKARE
HHHHHHHHHCCC
>Mature Secondary Structure
MFEHTTVLLKETVDGLNVKEDGTYVDCTLGGAGHSEYLLSQLSEKGKLVAFDQDETALRH
CCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEECCCHHHHHH
AKEKLADYEGQVVFIKSNFRYLKDRLNEIGITKVDGVLFDLGVSSPQLDTPERGFSYHHD
HHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCHHCCEEEECCCCCCCCCCCCCCCCCCCC
APLDMRMDQSAGLSAKEVVNEWPYEDLVKIFFKYGEEKFSKQIARKIEKAREQSPIETTG
CCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
QLVDIIKEAIPAPARRSGGHPAKRIFQAIRIAVNDELRVFEEALLQAIEVLKPGGRVSVI
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEE
TFHSLEDRICKSTFKDMSSPPELPHGLPVIPKEFEPKLKLVNRKPITASEEELEHNNRAR
EEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCHHHHHHCCHHH
SAKLRIAEKARE
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA