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The map label for this gene is moeB [H]

Identifier: 52785401

GI number: 52785401

Start: 1600637

End: 1601719

Strand: Direct

Name: moeB [H]

Synonym: BLi01640

Alternate gene names: 52785401

Gene position: 1600637-1601719 (Clockwise)

Preceding gene: 52785400

Following gene: 52785402

Centisome position: 37.91

GC content: 48.85

Gene sequence:

>1083_bases
GTGTTTTTCAAAGAACAGCAGACGGAAAAATGGTATGATATAGAAAAGGAAGTAAAGGGGATCATCCTGATGCATGAACG
GTATTCAAGACAAATCAGATATCAAAACATCGGACAGGCGGGTCAGGACAGAATCAGGCAGAGCCATGTTTTGATCGTCG
GGGCCGGGGCGCTTGGCACGTCAGCCGCTGAAGGGCTTGTGCGCGCAGGCATCGGCGCACTGTCTATTATAGACCGCGAT
TATGTGGAATGGAGCAACTTGCAAAGGCAGCAGCTCTACACAGAGAAAGACGCGGAGGAAAGGCTTCCGAAAGCGGCGGC
TGCAAAAGAACGTCTTTCCGAGATCAACAGCGGTGTTGAGCTTCGTACATATGTTGCAGACGGCACAGTTGAAACACTCA
GACCGCTGATCGAAGAGGCGGATGCCGTCATTGATGCAACGGATAACTTTGAGACCCGCATGATCATCAATGACCTTGCG
CAGGAGACGGGTACTCCGTGGGTTTACGGCGCTTGTGTCAGCAGTCAGGGCATGTATATGACGATTATTCCCCAAAAAAC
GCCATGCCTTGCCTGCCTGTTTGAAGATGTTCCGATTGGCGGGGCTACATGCGATACGTCGGGGATTATTTCGCCGGCCG
TTCAAATGGTGTCGGCTTATCAGCAAGCTGAAATTTTAAAAATCTTAACTGGACAGCAAGAAGCGCTTCAAACTGCTTTT
GTCACGTTTGATTTATGGGCGAACACCCATTATCGCATGCAGATCGGCCGCAATGAGAAATGTCCTTCGTGCGGAGAAAC
GCCGGTTTATCCTTTCCTCAGCAACATGAGCCAAAAGAAAGCCGACGTGCTGTGCGGAAGAGAGACCGTCCAAATTCGCA
GCGAAATGTTAAAACGGCTGCCTAAAGAGGAGCTCATGAAAAAGCTTTCGGTCATCGGAAAAGTGGATGCGAATGATTAT
TTGCTGCATGTACAATATGAGGCGTTCAGAATCGTTATTTTCAACGATGGAAGAGCCCTTGTTCACGGCACAAATGATAT
AAAAGAAGCCAACTCCATATTGGCGAGAGTCATCGGAATGTAA

Upstream 100 bases:

>100_bases
GGAATTTATAAATGTCAATAAAAAGTCGGATTTAGAAGAAATAGAGCCGTTCTTTCCGGGATGAAATATTTTATCATAGA
TTGCAAGGGTTTTTCGGAAG

Downstream 100 bases:

>100_bases
CAACAGGAGGTTTTTTTAAATGATTGAAAAAAGAACGCCCATTCCAGTTTTTGAAGCCGCTGCGCGTGTCTCTCAGCACC
AGAAGCTGGGAGATGCAGAG

Product: thiamine/molybdopterin biosynthesis MoeB-like protein

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MFFKEQQTEKWYDIEKEVKGIILMHERYSRQIRYQNIGQAGQDRIRQSHVLIVGAGALGTSAAEGLVRAGIGALSIIDRD
YVEWSNLQRQQLYTEKDAEERLPKAAAAKERLSEINSGVELRTYVADGTVETLRPLIEEADAVIDATDNFETRMIINDLA
QETGTPWVYGACVSSQGMYMTIIPQKTPCLACLFEDVPIGGATCDTSGIISPAVQMVSAYQQAEILKILTGQQEALQTAF
VTFDLWANTHYRMQIGRNEKCPSCGETPVYPFLSNMSQKKADVLCGRETVQIRSEMLKRLPKEELMKKLSVIGKVDANDY
LLHVQYEAFRIVIFNDGRALVHGTNDIKEANSILARVIGM

Sequences:

>Translated_360_residues
MFFKEQQTEKWYDIEKEVKGIILMHERYSRQIRYQNIGQAGQDRIRQSHVLIVGAGALGTSAAEGLVRAGIGALSIIDRD
YVEWSNLQRQQLYTEKDAEERLPKAAAAKERLSEINSGVELRTYVADGTVETLRPLIEEADAVIDATDNFETRMIINDLA
QETGTPWVYGACVSSQGMYMTIIPQKTPCLACLFEDVPIGGATCDTSGIISPAVQMVSAYQQAEILKILTGQQEALQTAF
VTFDLWANTHYRMQIGRNEKCPSCGETPVYPFLSNMSQKKADVLCGRETVQIRSEMLKRLPKEELMKKLSVIGKVDANDY
LLHVQYEAFRIVIFNDGRALVHGTNDIKEANSILARVIGM
>Mature_360_residues
MFFKEQQTEKWYDIEKEVKGIILMHERYSRQIRYQNIGQAGQDRIRQSHVLIVGAGALGTSAAEGLVRAGIGALSIIDRD
YVEWSNLQRQQLYTEKDAEERLPKAAAAKERLSEINSGVELRTYVADGTVETLRPLIEEADAVIDATDNFETRMIINDLA
QETGTPWVYGACVSSQGMYMTIIPQKTPCLACLFEDVPIGGATCDTSGIISPAVQMVSAYQQAEILKILTGQQEALQTAF
VTFDLWANTHYRMQIGRNEKCPSCGETPVYPFLSNMSQKKADVLCGRETVQIRSEMLKRLPKEELMKKLSVIGKVDANDY
LLHVQYEAFRIVIFNDGRALVHGTNDIKEANSILARVIGM

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=310, Percent_Identity=30.3225806451613, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI13376212, Length=184, Percent_Identity=32.6086956521739, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI38327032, Length=168, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787048, Length=241, Percent_Identity=33.195020746888, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI87082356, Length=246, Percent_Identity=34.9593495934959, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17540406, Length=246, Percent_Identity=30.4878048780488, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321903, Length=271, Percent_Identity=27.6752767527675, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=28.8235294117647, Blast_Score=84, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582879, Length=291, Percent_Identity=29.2096219931272, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24641311, Length=181, Percent_Identity=35.3591160220994, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 40295; Mature: 40295

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00036 BZIP_BASIC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFKEQQTEKWYDIEKEVKGIILMHERYSRQIRYQNIGQAGQDRIRQSHVLIVGAGALGT
CCCCCCCCHHHHHHHHHHCEEEEEEHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCC
SAAEGLVRAGIGALSIIDRDYVEWSNLQRQQLYTEKDAEERLPKAAAAKERLSEINSGVE
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCE
LRTYVADGTVETLRPLIEEADAVIDATDNFETRMIINDLAQETGTPWVYGACVSSQGMYM
EEEEEECCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEE
TIIPQKTPCLACLFEDVPIGGATCDTSGIISPAVQMVSAYQQAEILKILTGQQEALQTAF
EECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VTFDLWANTHYRMQIGRNEKCPSCGETPVYPFLSNMSQKKADVLCGRETVQIRSEMLKRL
EEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHC
PKEELMKKLSVIGKVDANDYLLHVQYEAFRIVIFNDGRALVHGTNDIKEANSILARVIGM
CHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEECCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFFKEQQTEKWYDIEKEVKGIILMHERYSRQIRYQNIGQAGQDRIRQSHVLIVGAGALGT
CCCCCCCCHHHHHHHHHHCEEEEEEHHHHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCC
SAAEGLVRAGIGALSIIDRDYVEWSNLQRQQLYTEKDAEERLPKAAAAKERLSEINSGVE
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCE
LRTYVADGTVETLRPLIEEADAVIDATDNFETRMIINDLAQETGTPWVYGACVSSQGMYM
EEEEEECCCHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEE
TIIPQKTPCLACLFEDVPIGGATCDTSGIISPAVQMVSAYQQAEILKILTGQQEALQTAF
EECCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VTFDLWANTHYRMQIGRNEKCPSCGETPVYPFLSNMSQKKADVLCGRETVQIRSEMLKRL
EEEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHC
PKEELMKKLSVIGKVDANDYLLHVQYEAFRIVIFNDGRALVHGTNDIKEANSILARVIGM
CHHHHHHHHHHHCCCCCCCEEEEEEEEEEEEEEEECCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]