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The map label for this gene is ptsI [H]

Identifier: 52785363

GI number: 52785363

Start: 1564464

End: 1566182

Strand: Direct

Name: ptsI [H]

Synonym: BLi01600

Alternate gene names: 52785363

Gene position: 1564464-1566182 (Clockwise)

Preceding gene: 52785362

Following gene: 52785364

Centisome position: 37.05

GC content: 45.9

Gene sequence:

>1719_bases
ATGCTTGAATTAAAAGGAATAGGCGCTTCAGCCGGTATTGCGATTGCAAAAGCATATCGGCTTGAGGAGCCTGATCTAAC
GGTTGAAAAGAAAAACATCTCTGATTCTGATGCGGAAGTAAATCGTTTTGAAGACGCCATCGAGCGTTCTAAGAAAGAGC
TTGAAACCATTAAAAACCATGCTTTGAAAGAGCTTGGCGAAGATAAAGCGGAAATCTTTTCTGCACATTTGTTAGTATTA
AGCGATCCTGAACTGTTAAACCCGATTAAAGACAAAATCAAATCTGATTCTGTCAATGCGGAATTCGCTTTAAAAGAAAC
GGCCGACATGTTCGTCACCATGTTCGAGTCGATGGACAATGAATATATGAAAGAACGCGCCGCGGACATCCGCGATGTCA
CCAAGCGTGTCACAGGGCATCTTCTCGGCGTTGAGATTCCGAATCCGAGCCTGATTTCCGAAGAAGTCATCATCGTGGCC
GAAGACTTGACACCGTCTGATACAGCGCAATTAAACCGCAACTTTGTAAAAGGGTTTACAACAGATATCGGCGGACGGAC
GTCCCACTCTGCCATCATGGCGCGCTCGCTTGAAATTCCGGCCGTTGTCGGCACAAAAGCGGCAACGAAAGGCATCCAAA
ACGGCGTAACAGTTATCGTTGACGGCATCAATGGCGATGTCATCGTTGATCCTTCCCAGGAAACAATCAGCGAATACGAA
GAAAAGCATCAAGCCTTCCTGGCGCAAAAAGCCGAATGGGCAAAGCTTGTCAACGAACCGACTGTAACGAGCGACGGCCA
TCACGTGGAACTTGCCGCTAATATCGGTACACCTGATGATGTAAAAGGCGTTTTGGAAAATGGAGGAGAAGCAGTCGGCC
TTTACCGAACTGAGTTCCTTTACATGGGCAGAGACCAGCTTCCGACAGAAGAGGAGCAATTTGATGCCTATAAAACCGTT
CTTGAGCGTATGGAAGGCAAATCCGTTGTCGTTCGTACTCTTGATATCGGCGGAGACAAAGAGCTTCCATATTTAGAACT
GCCAAAAGAAATGAATCCTTTCTTAGGATACAGAGCGATCCGTCTTTGCCTTGATGAGCAAGAGATTTTCAGAACGCAGC
TTCGTGCGCTGCTTCGGGCAAGTACATATGGAAACTTAAAAATTATGTTCCCTATGATCGCGACTGTAAACGAATTTAAA
GCAGCGAAAGCGATTCTTTTAGAAGAAAAAGAAAAGCTGAAAGCTGAAGGCCAACAAGTTTCCGACGACATTGAAGTCGG
TATGATGGTTGAAATCCCTTCAACAGCGGTCATCGCTGATCAATTTGCTAAAGAAGTCGATTTCTTCAGCATCGGAACGA
ATGACCTGATTCAATACACGATGGCTGCTGACCGGATGAATGAGCGGGTATCTTATCTTTACCAGCCATACAATCCTGCG
ATTCTCCGCCTGATCACGCTTGTCATTGAAGCGGCTCATAAAGAAGGAAAATGGGTCGGCATGTGCGGCGAAATGGCGGG
AGATGAAATTGCGATCCCGATCCTGCTGGGTCTTGGATTGGACGAGTTCTCAATGAGTGCGACGTCAATCCTGCCGGCAA
GAACGCAAATCAGCAAGCTTTCTAAAAAAGAGACAGAATCGTTCAAAGAGCAAATTCTGTCTATGAGCACAACAGAAGAG
GTTGTACAATTCGTAAAAGAAACCTTCAACCTGGCATAA

Upstream 100 bases:

>100_bases
CCAAAGGTGCTGAAATCACGATTTCTGCTTCCGGCGCTGATGAAAATGATGCTCTTTCCGCACTTGAAGAAACAATGAAA
AGCGAAGGTTTAGGCGAATA

Downstream 100 bases:

>100_bases
GCAATTGAAAATCCGGACCGGCATCACCAATGCCCGTCCGGGTTTTTTGTTTATCTGAATGATCCCGGCTGCGGCGGAGA
TAATACATGTGACCAATTGT

Product: PtsI

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MLELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSDAEVNRFEDAIERSKKELETIKNHALKELGEDKAEIFSAHLLVL
SDPELLNPIKDKIKSDSVNAEFALKETADMFVTMFESMDNEYMKERAADIRDVTKRVTGHLLGVEIPNPSLISEEVIIVA
EDLTPSDTAQLNRNFVKGFTTDIGGRTSHSAIMARSLEIPAVVGTKAATKGIQNGVTVIVDGINGDVIVDPSQETISEYE
EKHQAFLAQKAEWAKLVNEPTVTSDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEEEQFDAYKTV
LERMEGKSVVVRTLDIGGDKELPYLELPKEMNPFLGYRAIRLCLDEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFK
AAKAILLEEKEKLKAEGQQVSDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKKETESFKEQILSMSTTEE
VVQFVKETFNLA

Sequences:

>Translated_572_residues
MLELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSDAEVNRFEDAIERSKKELETIKNHALKELGEDKAEIFSAHLLVL
SDPELLNPIKDKIKSDSVNAEFALKETADMFVTMFESMDNEYMKERAADIRDVTKRVTGHLLGVEIPNPSLISEEVIIVA
EDLTPSDTAQLNRNFVKGFTTDIGGRTSHSAIMARSLEIPAVVGTKAATKGIQNGVTVIVDGINGDVIVDPSQETISEYE
EKHQAFLAQKAEWAKLVNEPTVTSDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEEEQFDAYKTV
LERMEGKSVVVRTLDIGGDKELPYLELPKEMNPFLGYRAIRLCLDEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFK
AAKAILLEEKEKLKAEGQQVSDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKKETESFKEQILSMSTTEE
VVQFVKETFNLA
>Mature_572_residues
MLELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSDAEVNRFEDAIERSKKELETIKNHALKELGEDKAEIFSAHLLVL
SDPELLNPIKDKIKSDSVNAEFALKETADMFVTMFESMDNEYMKERAADIRDVTKRVTGHLLGVEIPNPSLISEEVIIVA
EDLTPSDTAQLNRNFVKGFTTDIGGRTSHSAIMARSLEIPAVVGTKAATKGIQNGVTVIVDGINGDVIVDPSQETISEYE
EKHQAFLAQKAEWAKLVNEPTVTSDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFLYMGRDQLPTEEEQFDAYKTV
LERMEGKSVVVRTLDIGGDKELPYLELPKEMNPFLGYRAIRLCLDEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFK
AAKAILLEEKEKLKAEGQQVSDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKLSKKETESFKEQILSMSTTEE
VVQFVKETFNLA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=566, Percent_Identity=51.2367491166078, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI48994992, Length=514, Percent_Identity=37.7431906614786, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1788726, Length=518, Percent_Identity=37.2586872586873, Blast_Score=344, Evalue=8e-96,
Organism=Escherichia coli, GI1789193, Length=572, Percent_Identity=32.8671328671329, Blast_Score=307, Evalue=1e-84,
Organism=Escherichia coli, GI1787994, Length=430, Percent_Identity=29.3023255813954, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI226510935, Length=207, Percent_Identity=29.4685990338164, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63415; Mature: 63415

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSDAEVNRFEDAIERSKKELETIKNH
CEEECCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALKELGEDKAEIFSAHLLVLSDPELLNPIKDKIKSDSVNAEFALKETADMFVTMFESMDN
HHHHHCCHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHH
EYMKERAADIRDVTKRVTGHLLGVEIPNPSLISEEVIIVAEDLTPSDTAQLNRNFVKGFT
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TDIGGRTSHSAIMARSLEIPAVVGTKAATKGIQNGVTVIVDGINGDVIVDPSQETISEYE
HCCCCCCCHHHHHHHCCCCCEEECCHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHHH
EKHQAFLAQKAEWAKLVNEPTVTSDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEEE
YMGRDQLPTEEEQFDAYKTVLERMEGKSVVVRTLDIGGDKELPYLELPKEMNPFLGYRAI
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEECCHHCCHHHHHHHH
RLCLDEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFKAAKAILLEEKEKLKAEGQQV
HHHHCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
CCCCCCCEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKL
HHHHHHHHHHHHHCCCCEEEECHHCCCCCEEEEHHEECCCCHHHCCHHHHCCCHHHHHHH
SKKETESFKEQILSMSTTEEVVQFVKETFNLA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLELKGIGASAGIAIAKAYRLEEPDLTVEKKNISDSDAEVNRFEDAIERSKKELETIKNH
CEEECCCCCCCCHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ALKELGEDKAEIFSAHLLVLSDPELLNPIKDKIKSDSVNAEFALKETADMFVTMFESMDN
HHHHHCCHHHHHHHHEEEEECCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHH
EYMKERAADIRDVTKRVTGHLLGVEIPNPSLISEEVIIVAEDLTPSDTAQLNRNFVKGFT
HHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
TDIGGRTSHSAIMARSLEIPAVVGTKAATKGIQNGVTVIVDGINGDVIVDPSQETISEYE
HCCCCCCCHHHHHHHCCCCCEEECCHHHHHHHCCCCEEEEECCCCCEEECCCHHHHHHHH
EKHQAFLAQKAEWAKLVNEPTVTSDGHHVELAANIGTPDDVKGVLENGGEAVGLYRTEFL
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEEEEE
YMGRDQLPTEEEQFDAYKTVLERMEGKSVVVRTLDIGGDKELPYLELPKEMNPFLGYRAI
EECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCEECCHHCCHHHHHHHH
RLCLDEQEIFRTQLRALLRASTYGNLKIMFPMIATVNEFKAAKAILLEEKEKLKAEGQQV
HHHHCHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SDDIEVGMMVEIPSTAVIADQFAKEVDFFSIGTNDLIQYTMAADRMNERVSYLYQPYNPA
CCCCCCCEEEECCCHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
ILRLITLVIEAAHKEGKWVGMCGEMAGDEIAIPILLGLGLDEFSMSATSILPARTQISKL
HHHHHHHHHHHHHCCCCEEEECHHCCCCCEEEEHHEECCCCHHHCCHHHHCCCHHHHHHH
SKKETESFKEQILSMSTTEEVVQFVKETFNLA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7686067; 9384377; 10568751; 2497294; 8449881; 1495386 [H]