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The map label for this gene is ykvJ

Identifier: 52785292

GI number: 52785292

Start: 1501893

End: 1502552

Strand: Direct

Name: ykvJ

Synonym: BLi01527

Alternate gene names: 52785292

Gene position: 1501893-1502552 (Clockwise)

Preceding gene: 52785291

Following gene: 52785293

Centisome position: 35.57

GC content: 52.12

Gene sequence:

>660_bases
ATGAAACAGGAAAAAGCGATCGTCGTATTCAGCGGCGGTCAGGACAGTACGACTTGCCTATTGTGGGCGCTTCGGCAATT
TCAGGAGGTGGAGGCGGTCACGTTTCAATATAATCAGCGGCACAAGCAGGAAATCGAAGTCGCGAAAAAGATCGCTGCAA
AGCTTGGCGTCAAGCATCACCTTCTTGATATGGAACTGTTAAATCAGCTCGCGCCGAATGCTTTGACAAGGGATGATATC
GAGATTGAGGCGAAAGAAGGCGAGCTTCCGTCCACTTTTGTTCCGGGACGCAACCTCGTGTTTTTATCGTTCGCTTCGAT
CCTGGCCTACCAGGTCGGCGCGCGGCACATCATTACAGGCGTATGCGAGACAGACTTCAGCGGGTATCCGGATTGCCGTG
ATGAGTTCGTCAAATCCTGCAATGTCACTGTCAACTTGGCGATGGAGCGCCCGTTTGTCATCCATACGCCGCTTATGTGG
CTGAACAAGGCGGAAACGTGGGAGCTCGCCGATGAGCTTGATGCGCTTGATTTCGTGAAAAACGAGACGCTGACGTGCTA
CAACGGAATCATTGCCGACGGCTGCGGCGAATGCCCGGCCTGCAAGCTTCGCGCAAACGGCTACAATGAATATATGAAAA
TGAAAAAGGAGCGAGCTTAA

Upstream 100 bases:

>100_bases
GCTAGAGCACCTTTCTCACAGAGAAAGGTGTTTTTTCGTTTAGATATCATTGAATCTCTTCTTTTATGAAATGGTGACGA
AAAGCGGAGAAGGAGGAACT

Downstream 100 bases:

>100_bases
CAGTGCTTACACAAATTTATCCGCAACCAAGCCATCCTTACTCTTTTGAATTAAATAAAGATATGCAAATGTCAGCTGCA
CATTTTATTCCGAGAGAGGA

Product: YkvJ

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine synthase; PreQ(0) synthase; Queuosine biosynthesis protein queC

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKQEKAIVVFSGGQDSTTCLLWALRQFQEVEAVTFQYNQRHKQEIEVAKKIAAKLGVKHHLLDMELLNQLAPNALTRDDI
EIEAKEGELPSTFVPGRNLVFLSFASILAYQVGARHIITGVCETDFSGYPDCRDEFVKSCNVTVNLAMERPFVIHTPLMW
LNKAETWELADELDALDFVKNETLTCYNGIIADGCGECPACKLRANGYNEYMKMKKERA

Sequences:

>Translated_219_residues
MKQEKAIVVFSGGQDSTTCLLWALRQFQEVEAVTFQYNQRHKQEIEVAKKIAAKLGVKHHLLDMELLNQLAPNALTRDDI
EIEAKEGELPSTFVPGRNLVFLSFASILAYQVGARHIITGVCETDFSGYPDCRDEFVKSCNVTVNLAMERPFVIHTPLMW
LNKAETWELADELDALDFVKNETLTCYNGIIADGCGECPACKLRANGYNEYMKMKKERA
>Mature_219_residues
MKQEKAIVVFSGGQDSTTCLLWALRQFQEVEAVTFQYNQRHKQEIEVAKKIAAKLGVKHHLLDMELLNQLAPNALTRDDI
EIEAKEGELPSTFVPGRNLVFLSFASILAYQVGARHIITGVCETDFSGYPDCRDEFVKSCNVTVNLAMERPFVIHTPLMW
LNKAETWELADELDALDFVKNETLTCYNGIIADGCGECPACKLRANGYNEYMKMKKERA

Specific function: Catalyzes the ATP-dependent conversion of 7-carboxy-7- deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0))

COG id: COG0603

COG function: function code R; Predicted PP-loop superfamily ATPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queC family

Homologues:

Organism=Escherichia coli, GI1786648, Length=216, Percent_Identity=60.6481481481482, Blast_Score=276, Evalue=8e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEC_BACLD (Q65KI6)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078712.1
- RefSeq:   YP_091121.1
- ProteinModelPortal:   Q65KI6
- SMR:   Q65KI6
- STRING:   Q65KI6
- EnsemblBacteria:   EBBACT00000058269
- EnsemblBacteria:   EBBACT00000061959
- GeneID:   3030613
- GeneID:   3100502
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01527
- KEGG:   bli:BL03546
- NMPDR:   fig|279010.5.peg.1079
- eggNOG:   COG0603
- GeneTree:   EBGT00050000002556
- HOGENOM:   HBG553284
- OMA:   CETDYSG
- ProtClustDB:   CLSK887199
- BioCyc:   BLIC279010-1:BLI01527-MONOMER
- BioCyc:   BLIC279010:BL03546-MONOMER
- HAMAP:   MF_01633_B
- InterPro:   IPR018317
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF006293
- TIGRFAMs:   TIGR00364

Pfam domain/function: PF06508 ExsB

EC number: NA

Molecular weight: Translated: 24741; Mature: 24741

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEKAIVVFSGGQDSTTCLLWALRQFQEVEAVTFQYNQRHKQEIEVAKKIAAKLGVKHH
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LLDMELLNQLAPNALTRDDIEIEAKEGELPSTFVPGRNLVFLSFASILAYQVGARHIITG
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
VCETDFSGYPDCRDEFVKSCNVTVNLAMERPFVIHTPLMWLNKAETWELADELDALDFVK
HHCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEECCHHHHCCCCCHHHHHHHHHHHHHC
NETLTCYNGIIADGCGECPACKLRANGYNEYMKMKKERA
CCCEEEECCEECCCCCCCCCEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MKQEKAIVVFSGGQDSTTCLLWALRQFQEVEAVTFQYNQRHKQEIEVAKKIAAKLGVKHH
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
LLDMELLNQLAPNALTRDDIEIEAKEGELPSTFVPGRNLVFLSFASILAYQVGARHIITG
HHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHH
VCETDFSGYPDCRDEFVKSCNVTVNLAMERPFVIHTPLMWLNKAETWELADELDALDFVK
HHCCCCCCCCCHHHHHHHHCCCEEEEEECCCEEEECCHHHHCCCCCHHHHHHHHHHHHHC
NETLTCYNGIIADGCGECPACKLRANGYNEYMKMKKERA
CCCEEEECCEECCCCCCCCCEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA