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The map label for this gene is pbpE [H]

Identifier: 52785064

GI number: 52785064

Start: 1297047

End: 1298420

Strand: Direct

Name: pbpE [H]

Synonym: BLi01294

Alternate gene names: 52785064

Gene position: 1297047-1298420 (Clockwise)

Preceding gene: 52785063

Following gene: 52785066

Centisome position: 30.72

GC content: 43.67

Gene sequence:

>1374_bases
GTGAAACAGAACAAAAGAGAACAACTTGTTAACCTGTTTAGTGTGTTAAAAGAAAAAGGTCAGATCAACGCGGCAGTGCT
AGTCGCTGAAGACGGAGACATTCTTTATCAAGGCGCCTTCGGATATGAAAACTTGGCAGATAAGCGTTTATTGCAGGAAC
ATTCCTTATTTGAATTAGCATCGCTATCCAAGCCGTTCACTGCTTTGGGCATCCTTCAATTAGCCCAGCAAGGGGAATTG
AAAATCGAGGATCCCGTAGAACGGTGGATAACCGGGTTTCCCTACCAGGGCGTAACGATTCGCGATTTATTGACACATAC
GTCGGGTCTGCCCGATTATATGGAGTGGTTTCTACAACATTGGGATCATAACAAGATTGCTGTTAATCAAGATATCGTTG
ATATGCTGATGAATCATCATCCGCAACGCTATTTTGCCCCGGGTGAAGGTTGGCTTTACAGCAATACGGGATATGTGCTG
CTTGCGGTTATCATTGAAAAGGCATCAGGTCTGAGTTTTGCAGAATATATGAAAAAAAACATCTTTGAACCGGTGAAAAT
GCGGAACTCAAGGGTTTATAACAGAAGGCTGCAGCCTGAAACCATTGAGAACTATGCGTATGGATATGTGTATGACGTGC
ATACAGCACAGTACGTTCTGCCTGATGACCTTGAAGAAACAAAATATGTCTGCTATCTTGACGGGATTCAGGGGGACGGA
ACCGTAAACTCAACCATTCATGACTTATATTTGTTTGATCAAGCCTTATATACAGATTGTTTGATCAATCAAGTCTCAAA
AGAGCTTGCTTTTTCTCCCGCGTCTCTGAAAAACGGAGAGTCTATAGATTACGGGTGTGGATGGGTGCTTCGGGATAGTC
CTGAAACAGGGAGAATTGTCGGTCACAGCGGCGGCTGGCCAGGCTATTCAACCGTAATGATCCGATATATCGATCAGCAA
AAAACCTTCATTTATTTGAGCAATGTGGAACAGGATGATGAATATGAACAGGCCATCTTTGAAGCCGTTGAAAATATCCT
GTTTGACAAGGCCTACAAAATCCCTGATCGCCCTGCCGATAAAAAGAAAAAAGAGATCGATCCGGCCATTTACAAAAGAT
ATGTCGGAAGTTATTCACTTCAGGATGGCACCGCCGCTGATGTGACAGTTGATAAGGGCCGGATTTATATGCAAATCACC
GGACAGATCCGTTTTGAATTATTTCCAGCCTCGGAAACGCGCTTTTTTCTCAGGTCATTGTCTGTTGAAGTGGAATTTAT
TTTGGAGGATGGTGTCGCAACCCGGTTGATTATTTATCAAAATGGCGCAGAGGAAGAAGCGGTGCGGATAGTGGAAGCAA
ACAGCAAGGTATGA

Upstream 100 bases:

>100_bases
AATTGTAGAATAGTGATTTCAAAGACAAGAAGCCAGTCTTGTCTTTTTTTAGTTGAAACCTCATATTTCCATATGCGTAT
AAAGAAAGGGAGGTGGAAAA

Downstream 100 bases:

>100_bases
CCGTTTTGTTTGAGCGTTTGGCGGCCTGTGTCTGTTGTCTGTCAGAAGGAAATTTATCGTTTGAAGGTTGACTATGCAAT
TCGGGGATATCTGCCCGGCT

Product: PbpE

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: PBP 4*; PBP 4A; Penicillin-binding protein E [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKQNKREQLVNLFSVLKEKGQINAAVLVAEDGDILYQGAFGYENLADKRLLQEHSLFELASLSKPFTALGILQLAQQGEL
KIEDPVERWITGFPYQGVTIRDLLTHTSGLPDYMEWFLQHWDHNKIAVNQDIVDMLMNHHPQRYFAPGEGWLYSNTGYVL
LAVIIEKASGLSFAEYMKKNIFEPVKMRNSRVYNRRLQPETIENYAYGYVYDVHTAQYVLPDDLEETKYVCYLDGIQGDG
TVNSTIHDLYLFDQALYTDCLINQVSKELAFSPASLKNGESIDYGCGWVLRDSPETGRIVGHSGGWPGYSTVMIRYIDQQ
KTFIYLSNVEQDDEYEQAIFEAVENILFDKAYKIPDRPADKKKKEIDPAIYKRYVGSYSLQDGTAADVTVDKGRIYMQIT
GQIRFELFPASETRFFLRSLSVEVEFILEDGVATRLIIYQNGAEEEAVRIVEANSKV

Sequences:

>Translated_457_residues
MKQNKREQLVNLFSVLKEKGQINAAVLVAEDGDILYQGAFGYENLADKRLLQEHSLFELASLSKPFTALGILQLAQQGEL
KIEDPVERWITGFPYQGVTIRDLLTHTSGLPDYMEWFLQHWDHNKIAVNQDIVDMLMNHHPQRYFAPGEGWLYSNTGYVL
LAVIIEKASGLSFAEYMKKNIFEPVKMRNSRVYNRRLQPETIENYAYGYVYDVHTAQYVLPDDLEETKYVCYLDGIQGDG
TVNSTIHDLYLFDQALYTDCLINQVSKELAFSPASLKNGESIDYGCGWVLRDSPETGRIVGHSGGWPGYSTVMIRYIDQQ
KTFIYLSNVEQDDEYEQAIFEAVENILFDKAYKIPDRPADKKKKEIDPAIYKRYVGSYSLQDGTAADVTVDKGRIYMQIT
GQIRFELFPASETRFFLRSLSVEVEFILEDGVATRLIIYQNGAEEEAVRIVEANSKV
>Mature_457_residues
MKQNKREQLVNLFSVLKEKGQINAAVLVAEDGDILYQGAFGYENLADKRLLQEHSLFELASLSKPFTALGILQLAQQGEL
KIEDPVERWITGFPYQGVTIRDLLTHTSGLPDYMEWFLQHWDHNKIAVNQDIVDMLMNHHPQRYFAPGEGWLYSNTGYVL
LAVIIEKASGLSFAEYMKKNIFEPVKMRNSRVYNRRLQPETIENYAYGYVYDVHTAQYVLPDDLEETKYVCYLDGIQGDG
TVNSTIHDLYLFDQALYTDCLINQVSKELAFSPASLKNGESIDYGCGWVLRDSPETGRIVGHSGGWPGYSTVMIRYIDQQ
KTFIYLSNVEQDDEYEQAIFEAVENILFDKAYKIPDRPADKKKKEIDPAIYKRYVGSYSLQDGTAADVTVDKGRIYMQIT
GQIRFELFPASETRFFLRSLSVEVEFILEDGVATRLIIYQNGAEEEAVRIVEANSKV

Specific function: Probably involved in peptidoglycan modification during cortex synthesis [H]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI1790593, Length=272, Percent_Identity=29.0441176470588, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI87082101, Length=351, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17550934, Length=316, Percent_Identity=24.6835443037975, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI115533026, Length=368, Percent_Identity=20.9239130434783, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR021860 [H]

Pfam domain/function: PF00144 Beta-lactamase; PF11954 DUF3471 [H]

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 52284; Mature: 52284

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQNKREQLVNLFSVLKEKGQINAAVLVAEDGDILYQGAFGYENLADKRLLQEHSLFELA
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHH
SLSKPFTALGILQLAQQGELKIEDPVERWITGFPYQGVTIRDLLTHTSGLPDYMEWFLQH
HCCCCHHHHHHHHHHHCCCCEECCHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
WDHNKIAVNQDIVDMLMNHHPQRYFAPGEGWLYSNTGYVLLAVIIEKASGLSFAEYMKKN
CCCCCEEECHHHHHHHHHCCCCEEECCCCCEEECCCCCEEEEEHHHHCCCCCHHHHHHHH
IFEPVKMRNSRVYNRRLQPETIENYAYGYVYDVHTAQYVLPDDLEETKYVCYLDGIQGDG
CCCHHHHHCCHHHHCCCCCHHHHCCCCEEEEEECCEEEECCCCCCCCEEEEEEECCCCCC
TVNSTIHDLYLFDQALYTDCLINQVSKELAFSPASLKNGESIDYGCGWVLRDSPETGRIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCEEE
GHSGGWPGYSTVMIRYIDQQKTFIYLSNVEQDDEYEQAIFEAVENILFDKAYKIPDRPAD
ECCCCCCCHHEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KKKKEIDPAIYKRYVGSYSLQDGTAADVTVDKGRIYMQITGQIRFELFPASETRFFLRSL
CHHHHCCHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEEEEEEEEEEECCCHHHHHHHHH
SVEVEFILEDGVATRLIIYQNGAEEEAVRIVEANSKV
CEEEEEEEECCCEEEEEEEECCCCHHHEEEEECCCCC
>Mature Secondary Structure
MKQNKREQLVNLFSVLKEKGQINAAVLVAEDGDILYQGAFGYENLADKRLLQEHSLFELA
CCCCHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEECCCCCCHHHHHHHHHHHHHHHHH
SLSKPFTALGILQLAQQGELKIEDPVERWITGFPYQGVTIRDLLTHTSGLPDYMEWFLQH
HCCCCHHHHHHHHHHHCCCCEECCHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHH
WDHNKIAVNQDIVDMLMNHHPQRYFAPGEGWLYSNTGYVLLAVIIEKASGLSFAEYMKKN
CCCCCEEECHHHHHHHHHCCCCEEECCCCCEEECCCCCEEEEEHHHHCCCCCHHHHHHHH
IFEPVKMRNSRVYNRRLQPETIENYAYGYVYDVHTAQYVLPDDLEETKYVCYLDGIQGDG
CCCHHHHHCCHHHHCCCCCHHHHCCCCEEEEEECCEEEECCCCCCCCEEEEEEECCCCCC
TVNSTIHDLYLFDQALYTDCLINQVSKELAFSPASLKNGESIDYGCGWVLRDSPETGRIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCCCCEEE
GHSGGWPGYSTVMIRYIDQQKTFIYLSNVEQDDEYEQAIFEAVENILFDKAYKIPDRPAD
ECCCCCCCHHEEEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
KKKKEIDPAIYKRYVGSYSLQDGTAADVTVDKGRIYMQITGQIRFELFPASETRFFLRSL
CHHHHCCHHHHHHHHCCCCCCCCCEEEEEEECCEEEEEEEEEEEEEEECCCHHHHHHHHH
SVEVEFILEDGVATRLIIYQNGAEEEAVRIVEANSKV
CEEEEEEEECCCEEEEEEEECCCCHHHEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8491712; 9384377 [H]