Click here to switch to the map view.

The map label for this gene is ppnK

Identifier: 52785026

GI number: 52785026

Start: 1266562

End: 1267365

Strand: Direct

Name: ppnK

Synonym: BLi01255

Alternate gene names: 52785026

Gene position: 1266562-1267365 (Clockwise)

Preceding gene: 52785025

Following gene: 52785027

Centisome position: 29.99

GC content: 49.0

Gene sequence:

>804_bases
ATGATGAAGTTTGCGGTATCGTCCAAAGGAAATGCGGTTTCTGATTCATTGAAAAGCAAAATTCAGACGTATCTGCTCGA
CTTCGGTCTCGAATGCGATGAAGAAGAGCCGGATATCGTCATTTCCGTCGGCGGTGACGGAACGCTCTTATACGCATTTC
ACAAATACAGCGGCCGCCTCGACAAAACGGCTTTTGTCGGGGTTCATACCGGGCATCTCGGCTTTTATGCCGACTGGGTC
CCGAGTGAGATTGAAAAGCTCGTGATCGCGATTGCGAAAACGCCGTATCAAATTGTTGAATATCCGGTTTTGGAAGTCAT
CGTCCGCTATAATGACGGCAGCGATGAAGCGAGATATTTGGCGCTGAACGAATGTACGATCAAGAGCATTGAAGGAACGC
TTGTGACGGATGTCGAGATTAAAGGGGAACTTTTTGAAACGTTCCGCGGCGACGGCCTTTGTCTGTCGACTCCTTCCGGA
AGTACGGCATATAACAAGGCGCTTGGCGGTGCGATTATTCATCCGTCCATCAGGGCGATCCAGCTGGCTGAAATGGCGTC
GATCAACAACCGGGTGTTTCGGACGGTCGGATCGCCGCTCATTCTGCCTGAGCATCACACTTGTTTAATTAAACCGATAA
ATGATGTGACGTTTCAAGTCGCGATTGACCACTTGACATTGCTGCATAAAGACGTCAAGTCGATTCAATGCCGCGTCGCA
AACGAAAATATCAGGTTTGCCCGCTTTAGGCCGTTTCCGTTTTGGAAAAGGGTCCAGGATTCCTTTATCGGAAAAGGAGA
ATAG

Upstream 100 bases:

>100_bases
ATATGGAAATGTCTGAGATCAGGGGCGAGATTCAGGAGGCTCAGGCGGCGTTTTCACGCAAGAAAAAGGATGAGGATGAC
GGTTAGGGGAGTGAGATGGA

Downstream 100 bases:

>100_bases
GAAGGTTTGCTGTGAGCGATTTTTTGATGAAACAGATCATAACAGAAGAGGACAGCGGGGTTCTTTTGAAAGAATACGTC
CAGCGGCTGGGCATTTCAAA

Product: inorganic polyphosphate/ATP-NAD kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase 1

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MMKFAVSSKGNAVSDSLKSKIQTYLLDFGLECDEEEPDIVISVGGDGTLLYAFHKYSGRLDKTAFVGVHTGHLGFYADWV
PSEIEKLVIAIAKTPYQIVEYPVLEVIVRYNDGSDEARYLALNECTIKSIEGTLVTDVEIKGELFETFRGDGLCLSTPSG
STAYNKALGGAIIHPSIRAIQLAEMASINNRVFRTVGSPLILPEHHTCLIKPINDVTFQVAIDHLTLLHKDVKSIQCRVA
NENIRFARFRPFPFWKRVQDSFIGKGE

Sequences:

>Translated_267_residues
MMKFAVSSKGNAVSDSLKSKIQTYLLDFGLECDEEEPDIVISVGGDGTLLYAFHKYSGRLDKTAFVGVHTGHLGFYADWV
PSEIEKLVIAIAKTPYQIVEYPVLEVIVRYNDGSDEARYLALNECTIKSIEGTLVTDVEIKGELFETFRGDGLCLSTPSG
STAYNKALGGAIIHPSIRAIQLAEMASINNRVFRTVGSPLILPEHHTCLIKPINDVTFQVAIDHLTLLHKDVKSIQCRVA
NENIRFARFRPFPFWKRVQDSFIGKGE
>Mature_267_residues
MMKFAVSSKGNAVSDSLKSKIQTYLLDFGLECDEEEPDIVISVGGDGTLLYAFHKYSGRLDKTAFVGVHTGHLGFYADWV
PSEIEKLVIAIAKTPYQIVEYPVLEVIVRYNDGSDEARYLALNECTIKSIEGTLVTDVEIKGELFETFRGDGLCLSTPSG
STAYNKALGGAIIHPSIRAIQLAEMASINNRVFRTVGSPLILPEHHTCLIKPINDVTFQVAIDHLTLLHKDVKSIQCRVA
NENIRFARFRPFPFWKRVQDSFIGKGE

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Escherichia coli, GI1788968, Length=152, Percent_Identity=32.8947368421053, Blast_Score=66, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6325068, Length=236, Percent_Identity=26.271186440678, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK1_BACLD (Q65LA2)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078455.2
- RefSeq:   YP_090855.1
- ProteinModelPortal:   Q65LA2
- SMR:   Q65LA2
- STRING:   Q65LA2
- EnsemblBacteria:   EBBACT00000058071
- EnsemblBacteria:   EBBACT00000059611
- GeneID:   3030258
- GeneID:   3098767
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01255
- KEGG:   bli:BL05111
- NMPDR:   fig|279010.5.peg.2472
- eggNOG:   COG0061
- GeneTree:   EBGT00050000002705
- HOGENOM:   HBG314020
- ProtClustDB:   PRK04885
- BioCyc:   BLIC279010-1:BLI01255-MONOMER
- BioCyc:   BLIC279010:BL05111-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 29670; Mature: 29670

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKFAVSSKGNAVSDSLKSKIQTYLLDFGLECDEEEPDIVISVGGDGTLLYAFHKYSGRL
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCC
DKTAFVGVHTGHLGFYADWVPSEIEKLVIAIAKTPYQIVEYPVLEVIVRYNDGSDEARYL
CCEEEEEEEECCCCEEECCCHHHHHHHHHHHHCCCHHHHHCCEEEEEEEECCCCCCEEEE
ALNECTIKSIEGTLVTDVEIKGELFETFRGDGLCLSTPSGSTAYNKALGGAIIHPSIRAI
EEECCEEEECCCEEEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHHCCCEEECCCHHEE
QLAEMASINNRVFRTVGSPLILPEHHTCLIKPINDVTFQVAIDHLTLLHKDVKSIQCRVA
HHHHHHHHCHHHHHHCCCCEEECCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHEEEEEE
NENIRFARFRPFPFWKRVQDSFIGKGE
CCCEEEEEECCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMKFAVSSKGNAVSDSLKSKIQTYLLDFGLECDEEEPDIVISVGGDGTLLYAFHKYSGRL
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEEEECCCCCC
DKTAFVGVHTGHLGFYADWVPSEIEKLVIAIAKTPYQIVEYPVLEVIVRYNDGSDEARYL
CCEEEEEEEECCCCEEECCCHHHHHHHHHHHHCCCHHHHHCCEEEEEEEECCCCCCEEEE
ALNECTIKSIEGTLVTDVEIKGELFETFRGDGLCLSTPSGSTAYNKALGGAIIHPSIRAI
EEECCEEEECCCEEEEEEEECHHHHHHHCCCCEEEECCCCCCHHHHHCCCEEECCCHHEE
QLAEMASINNRVFRTVGSPLILPEHHTCLIKPINDVTFQVAIDHLTLLHKDVKSIQCRVA
HHHHHHHHCHHHHHHCCCCEEECCCCEEEEEECCCEEEEEHHHHHHHHHHHHHHEEEEEE
NENIRFARFRPFPFWKRVQDSFIGKGE
CCCEEEEEECCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA