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The map label for this gene is oppA [H]

Identifier: 52785004

GI number: 52785004

Start: 1244233

End: 1245858

Strand: Direct

Name: oppA [H]

Synonym: BLi01232

Alternate gene names: 52785004

Gene position: 1244233-1245858 (Clockwise)

Preceding gene: 52785002

Following gene: 52785005

Centisome position: 29.47

GC content: 45.69

Gene sequence:

>1626_bases
TTGAAGAAGCGTTTGTCATTTATCAGTTTAATGCTCATTTTCACACTCGTCCTCAGCGCCTGCGGCTTCGGCTCAAGCTC
CGGTGACGGCGGTAAAAAAGACAGCAAAGGGAAAGACACATTAAATGTCAACATTAAAACAGAACCGTTTTCACTACATC
CGGGACTCGCAAACGATTCGGTGTCTGCAAACGTGCTTCGTCAGACTTTTGAAGGATTGACGACAATCGGTAAAGATGGA
AAGCCGGTTGAAGCGGCAGCCGAAAAAATCGAAGTCAGCGACGACCAAAAAACATACACATTCACGCTCCGCGACGCGAA
ATGGTCAAATGGAGATCCTGTAACAGCAGAGGATTTTGAATACGCATGGAAATGGGCGCTCGACCCTAAAAACGAATCGC
AATATGCGTATCAGCTTTACTACTTAAAAGGCGGAGAAGCAGCGAACACCGGCAAAGGGAAAATTGAAGATGTCGGCGTT
AAAGCTGTTAATGATAAGACTTTAAAAGTCGAGCTTGAAAAACCGACACCGTATTTTACTGAACTGACAGCATTCTACAC
ATATATGCCAGTCAATAAAAAGGTAGCAGAGAAGAATGCGAAATGGTACACAAACGCAGATGAGAACTACGTATCTAACG
GACCTTTCAAAATGGCGAAATGGAAGCACAGCGGAAACATCGTACTGGAGAAAAACGACCAGTACTGGGATAAAGACGCT
GTTAAGCTGAAGAAAATCAATATGGCGATGGTCAACGATCCGAACACTGGTCTGAACATGTACAAAAAAGGCGAGCTAGA
CTTTGTAGGACAGCCGCTTGACCAGATTTCAACGGATGCGATTCCAAGCCTGAAAAAAGAAGGCCTGAACATTGATCCGT
TCGCATCGGTTTACCTGTACAAATTCAACACTGAAGCGGCTCCGCTGAACAATGTCAACATCCGTAAGGCGCTGACATAC
GCGATCAACCGCGAAGCGATCGTCAAAAACATCACGCAAGCGGAACAGCTGCCTGCGATGGGATTAGTGCCGCCGGCGGT
CCACGGCTTTGAATCCAACAAAGGCTATTTCAAAGACCATGATGTCGATAAGGCGAAAGAACACTTGGAAAAAGGCTTGA
AAGAGCTCGGTTTGAAAAAAGCGTCAGACCTTCCGAAAATCACGCTTTCCTACAACACGGATGAAGCGCACCAAAAAATC
GCTCAAGCCGTTCAGGAAATGTGGAACAAGGAACTGGGCGTAGATGTGGAATTAGGCAATGAAGAATGGAACGTTTACAT
CGATAAGCTCCATGCCGGAAACTATCAAATCGGCCGTTTAGGCTGGACCGCCGACTTTAACGACGGGATGAACTTCCTTG
AAACATACCGCGACAAAGAAGGCGGAAACAATGATACGAACTGGGAAAACGCGAAGTATAAAGAGCTGCTGAATAAAGCA
TCAAGAGAAACTGATTCAGCGAAACGCATCGAGCTGATGAAAGAAGCGGAAAGCATCATCATGGATGAGCTGCCGGTCGC
ACCGATCTACTTCTACACAATGCCGTATCTTCATGATGAAAGCTTAAAAGACTTTGTTCTGACCGGTACAGGTGAGATCT
ATTTCAAAACCGCGCATTTTGAATAA

Upstream 100 bases:

>100_bases
GAGGAAAAAGTATGGCAAGTGGGCCTATTACTTTTTTATGATAAATCTGAATGTTCAGGAGTAAGGAACTAAAAAAACAA
GAAATTGGGGGGGTTTCTCT

Downstream 100 bases:

>100_bases
AATTGCATGAAAGTATATGGGGCTTAGACTTTCCCCCCATATACTTTTATTTATGAAAAGAAGTTAATAGACTGAATATT
CGGAGGTGTGTAAAATTGAT

Product: hypothetical protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: Stage 0 sporulation protein KA [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKKRLSFISLMLIFTLVLSACGFGSSSGDGGKKDSKGKDTLNVNIKTEPFSLHPGLANDSVSANVLRQTFEGLTTIGKDG
KPVEAAAEKIEVSDDQKTYTFTLRDAKWSNGDPVTAEDFEYAWKWALDPKNESQYAYQLYYLKGGEAANTGKGKIEDVGV
KAVNDKTLKVELEKPTPYFTELTAFYTYMPVNKKVAEKNAKWYTNADENYVSNGPFKMAKWKHSGNIVLEKNDQYWDKDA
VKLKKINMAMVNDPNTGLNMYKKGELDFVGQPLDQISTDAIPSLKKEGLNIDPFASVYLYKFNTEAAPLNNVNIRKALTY
AINREAIVKNITQAEQLPAMGLVPPAVHGFESNKGYFKDHDVDKAKEHLEKGLKELGLKKASDLPKITLSYNTDEAHQKI
AQAVQEMWNKELGVDVELGNEEWNVYIDKLHAGNYQIGRLGWTADFNDGMNFLETYRDKEGGNNDTNWENAKYKELLNKA
SRETDSAKRIELMKEAESIIMDELPVAPIYFYTMPYLHDESLKDFVLTGTGEIYFKTAHFE

Sequences:

>Translated_541_residues
MKKRLSFISLMLIFTLVLSACGFGSSSGDGGKKDSKGKDTLNVNIKTEPFSLHPGLANDSVSANVLRQTFEGLTTIGKDG
KPVEAAAEKIEVSDDQKTYTFTLRDAKWSNGDPVTAEDFEYAWKWALDPKNESQYAYQLYYLKGGEAANTGKGKIEDVGV
KAVNDKTLKVELEKPTPYFTELTAFYTYMPVNKKVAEKNAKWYTNADENYVSNGPFKMAKWKHSGNIVLEKNDQYWDKDA
VKLKKINMAMVNDPNTGLNMYKKGELDFVGQPLDQISTDAIPSLKKEGLNIDPFASVYLYKFNTEAAPLNNVNIRKALTY
AINREAIVKNITQAEQLPAMGLVPPAVHGFESNKGYFKDHDVDKAKEHLEKGLKELGLKKASDLPKITLSYNTDEAHQKI
AQAVQEMWNKELGVDVELGNEEWNVYIDKLHAGNYQIGRLGWTADFNDGMNFLETYRDKEGGNNDTNWENAKYKELLNKA
SRETDSAKRIELMKEAESIIMDELPVAPIYFYTMPYLHDESLKDFVLTGTGEIYFKTAHFE
>Mature_541_residues
MKKRLSFISLMLIFTLVLSACGFGSSSGDGGKKDSKGKDTLNVNIKTEPFSLHPGLANDSVSANVLRQTFEGLTTIGKDG
KPVEAAAEKIEVSDDQKTYTFTLRDAKWSNGDPVTAEDFEYAWKWALDPKNESQYAYQLYYLKGGEAANTGKGKIEDVGV
KAVNDKTLKVELEKPTPYFTELTAFYTYMPVNKKVAEKNAKWYTNADENYVSNGPFKMAKWKHSGNIVLEKNDQYWDKDA
VKLKKINMAMVNDPNTGLNMYKKGELDFVGQPLDQISTDAIPSLKKEGLNIDPFASVYLYKFNTEAAPLNNVNIRKALTY
AINREAIVKNITQAEQLPAMGLVPPAVHGFESNKGYFKDHDVDKAKEHLEKGLKELGLKKASDLPKITLSYNTDEAHQKI
AQAVQEMWNKELGVDVELGNEEWNVYIDKLHAGNYQIGRLGWTADFNDGMNFLETYRDKEGGNNDTNWENAKYKELLNKA
SRETDSAKRIELMKEAESIIMDELPVAPIYFYTMPYLHDESLKDFVLTGTGEIYFKTAHFE

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, It binds peptides up to five amino acids long with high affinity. Also required for sporulation and competence [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=482, Percent_Identity=33.195020746888, Blast_Score=267, Evalue=2e-72,
Organism=Escherichia coli, GI1789397, Length=516, Percent_Identity=33.1395348837209, Blast_Score=265, Evalue=6e-72,
Organism=Escherichia coli, GI87081878, Length=514, Percent_Identity=32.4902723735409, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1789966, Length=481, Percent_Identity=27.4428274428274, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI1787762, Length=555, Percent_Identity=25.5855855855856, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1787052, Length=538, Percent_Identity=25.6505576208178, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789887, Length=471, Percent_Identity=27.8131634819533, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787551, Length=488, Percent_Identity=24.7950819672131, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60977; Mature: 60977

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLSFISLMLIFTLVLSACGFGSSSGDGGKKDSKGKDTLNVNIKTEPFSLHPGLANDS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCEEECCCCCCCC
VSANVLRQTFEGLTTIGKDGKPVEAAAEKIEVSDDQKTYTFTLRDAKWSNGDPVTAEDFE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHEECCCCCEEEEEEEECCCCCCCCCCCCCCCC
YAWKWALDPKNESQYAYQLYYLKGGEAANTGKGKIEDVGVKAVNDKTLKVELEKPTPYFT
EEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCEEECCEEEECCCEEEEEECCCCCHHH
ELTAFYTYMPVNKKVAEKNAKWYTNADENYVSNGPFKMAKWKHSGNIVLEKNDQYWDKDA
HHHHHHEECCCCHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCC
VKLKKINMAMVNDPNTGLNMYKKGELDFVGQPLDQISTDAIPSLKKEGLNIDPFASVYLY
EEEEEEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCEEEEE
KFNTEAAPLNNVNIRKALTYAINREAIVKNITQAEQLPAMGLVPPAVHGFESNKGYFKDH
EECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCC
DVDKAKEHLEKGLKELGLKKASDLPKITLSYNTDEAHQKIAQAVQEMWNKELGVDVELGN
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCHHCCEEEEECC
EEWNVYIDKLHAGNYQIGRLGWTADFNDGMNFLETYRDKEGGNNDTNWENAKYKELLNKA
CCEEEEEEEEECCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
SRETDSAKRIELMKEAESIIMDELPVAPIYFYTMPYLHDESLKDFVLTGTGEIYFKTAHF
HCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEEECC
E
C
>Mature Secondary Structure
MKKRLSFISLMLIFTLVLSACGFGSSSGDGGKKDSKGKDTLNVNIKTEPFSLHPGLANDS
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEECCEEECCCCCCCC
VSANVLRQTFEGLTTIGKDGKPVEAAAEKIEVSDDQKTYTFTLRDAKWSNGDPVTAEDFE
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHEECCCCCEEEEEEEECCCCCCCCCCCCCCCC
YAWKWALDPKNESQYAYQLYYLKGGEAANTGKGKIEDVGVKAVNDKTLKVELEKPTPYFT
EEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCEEECCEEEECCCEEEEEECCCCCHHH
ELTAFYTYMPVNKKVAEKNAKWYTNADENYVSNGPFKMAKWKHSGNIVLEKNDQYWDKDA
HHHHHHEECCCCHHHHHCCCCEEECCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCCC
VKLKKINMAMVNDPNTGLNMYKKGELDFVGQPLDQISTDAIPSLKKEGLNIDPFASVYLY
EEEEEEEEEEECCCCCCCCCEECCCCCCCCCCHHHHHHHCCCHHHHCCCCCCCCCEEEEE
KFNTEAAPLNNVNIRKALTYAINREAIVKNITQAEQLPAMGLVPPAVHGFESNKGYFKDH
EECCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCC
DVDKAKEHLEKGLKELGLKKASDLPKITLSYNTDEAHQKIAQAVQEMWNKELGVDVELGN
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCHHCCEEEEECC
EEWNVYIDKLHAGNYQIGRLGWTADFNDGMNFLETYRDKEGGNNDTNWENAKYKELLNKA
CCEEEEEEEEECCCEEEEEEEEECCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHH
SRETDSAKRIELMKEAESIIMDELPVAPIYFYTMPYLHDESLKDFVLTGTGEIYFKTAHF
HCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEECCCEEEEEEECC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1901616; 1899858; 9384377 [H]