Click here to switch to the map view.

The map label for this gene is argF [H]

Identifier: 52784984

GI number: 52784984

Start: 1225000

End: 1225950

Strand: Direct

Name: argF [H]

Synonym: BLi01212

Alternate gene names: 52784984

Gene position: 1225000-1225950 (Clockwise)

Preceding gene: 52784983

Following gene: 52784986

Centisome position: 29.01

GC content: 48.79

Gene sequence:

>951_bases
ATGTCTACTGTAAACATCGGGGCGACCCGGGAGATCAATCTGTCAGGCAAGGATTTCTTAAGCCTGAAAGACTTTTCAAA
AGAGGAGATCGTCTATTTGCTGAAAGAGGCAGCGGCAATGAAGCGAAATAAAATTCAAGACATCTTTAAAGGAAAAACAT
TGGCGATGATCTTTGAAAAATCGTCGACGAGAACGCGCGTATCGTTTGAAGCCGGAATGGCGCAGCTCGGCGGAAACGCC
CTGTTTTTAAGCAGTAAAGACATGCAGCTGGGCAGAGGGGAAACGATCGCAGATACCGCGAAAGTGCTTTCGCGATATGT
GGACGCGATTATGATCCGCACATTCGAACACGAAAAAGTCGAAGAGCTTGCCAAGCACGCCGAGATTCCCGTGATCAACG
GATTGACCGATCTTTTTCATCCGTGTCAAGCGCTTAGTGACCTATTAACGATTCAAGAGATGAAAGGAACATTAAAAGGG
CTGAAAGCCGTTTATGTTGGCGACGGAAACAATGTCGCGCATTCGCTGTTAATCGGCTGCGCGAAAGTGGGATGCGACAT
TACGGTGGCTTCACCCAAAGGGTATGAGCCGAATCAGGACGTGGTGAAACTGGCCGAGACATTCGCCGAAGAATCCGGTG
CGGCCGTCACGATCACAAATGATCCCGTGTCAGCCGTTCAACAAGCGGATGTCATCTATTCTGATGTTTTTACAAGCATG
GGACAGGAGGAGGAAGCCGAAGAACGGATGAAGATTTTTGCTCCTTACCAAGTCAACGGAACGCTTGTCTCCCATGCCAA
GCCTGATTACATCTTTCTGCACTGCCTTCCTGCCCACCGGGAGGAAGAGGTCACCGCAGAGATCATTGACGGTCCGAATT
CAGCGGTATTTCAGCAGGCTGAAAACCGCCTGCACGTCCAAAAAGCTTTATTAAAAGCACTGTTATCTTAA

Upstream 100 bases:

>100_bases
TGATGACGGAAGAAGAGACGGTGCGGGCATTTTTGACGAGCGGGCATGGAACGACTAAAGCCGTGTCGCTTAAAGATTTA
TATAAAAAGGAAGTGGAGAC

Downstream 100 bases:

>100_bases
ATGAAAAACAGCTGATCCATTGATCAGCTGTTTTTAGGCGAATATCAAAACGGCGATGCCGATGACAAAGCAGTAAATCG
AGAAATAAACCAAGTTTCCT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase [H]

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSTVNIGATREINLSGKDFLSLKDFSKEEIVYLLKEAAAMKRNKIQDIFKGKTLAMIFEKSSTRTRVSFEAGMAQLGGNA
LFLSSKDMQLGRGETIADTAKVLSRYVDAIMIRTFEHEKVEELAKHAEIPVINGLTDLFHPCQALSDLLTIQEMKGTLKG
LKAVYVGDGNNVAHSLLIGCAKVGCDITVASPKGYEPNQDVVKLAETFAEESGAAVTITNDPVSAVQQADVIYSDVFTSM
GQEEEAEERMKIFAPYQVNGTLVSHAKPDYIFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLHVQKALLKALLS

Sequences:

>Translated_316_residues
MSTVNIGATREINLSGKDFLSLKDFSKEEIVYLLKEAAAMKRNKIQDIFKGKTLAMIFEKSSTRTRVSFEAGMAQLGGNA
LFLSSKDMQLGRGETIADTAKVLSRYVDAIMIRTFEHEKVEELAKHAEIPVINGLTDLFHPCQALSDLLTIQEMKGTLKG
LKAVYVGDGNNVAHSLLIGCAKVGCDITVASPKGYEPNQDVVKLAETFAEESGAAVTITNDPVSAVQQADVIYSDVFTSM
GQEEEAEERMKIFAPYQVNGTLVSHAKPDYIFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLHVQKALLKALLS
>Mature_315_residues
STVNIGATREINLSGKDFLSLKDFSKEEIVYLLKEAAAMKRNKIQDIFKGKTLAMIFEKSSTRTRVSFEAGMAQLGGNAL
FLSSKDMQLGRGETIADTAKVLSRYVDAIMIRTFEHEKVEELAKHAEIPVINGLTDLFHPCQALSDLLTIQEMKGTLKGL
KAVYVGDGNNVAHSLLIGCAKVGCDITVASPKGYEPNQDVVKLAETFAEESGAAVTITNDPVSAVQQADVIYSDVFTSMG
QEEEAEERMKIFAPYQVNGTLVSHAKPDYIFLHCLPAHREEEVTAEIIDGPNSAVFQQAENRLHVQKALLKALLS

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI38788445, Length=311, Percent_Identity=45.3376205787781, Blast_Score=293, Evalue=1e-79,
Organism=Homo sapiens, GI18105007, Length=315, Percent_Identity=30.7936507936508, Blast_Score=135, Evalue=3e-32,
Organism=Escherichia coli, GI1790703, Length=327, Percent_Identity=39.4495412844037, Blast_Score=217, Evalue=8e-58,
Organism=Escherichia coli, GI1786469, Length=331, Percent_Identity=39.2749244712991, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI2367364, Length=313, Percent_Identity=32.9073482428115, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI48994908, Length=375, Percent_Identity=25.6, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=335, Percent_Identity=30.1492537313433, Blast_Score=107, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6322373, Length=310, Percent_Identity=39.3548387096774, Blast_Score=230, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=315, Percent_Identity=31.4285714285714, Blast_Score=121, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24642586, Length=311, Percent_Identity=29.5819935691318, Blast_Score=116, Evalue=2e-26,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.3 [H]

Molecular weight: Translated: 34596; Mature: 34465

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVNIGATREINLSGKDFLSLKDFSKEEIVYLLKEAAAMKRNKIQDIFKGKTLAMIFEK
CCCEECCCEEEECCCCCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
SSTRTRVSFEAGMAQLGGNALFLSSKDMQLGRGETIADTAKVLSRYVDAIMIRTFEHEKV
CCCCEEEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
EELAKHAEIPVINGLTDLFHPCQALSDLLTIQEMKGTLKGLKAVYVGDGNNVAHSLLIGC
HHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
AKVGCDITVASPKGYEPNQDVVKLAETFAEESGAAVTITNDPVSAVQQADVIYSDVFTSM
HHHCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
GQEEEAEERMKIFAPYQVNGTLVSHAKPDYIFLHCLPAHREEEVTAEIIDGPNSAVFQQA
CCHHHHHHHHHEECCEEECCEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHH
ENRLHVQKALLKALLS
HHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STVNIGATREINLSGKDFLSLKDFSKEEIVYLLKEAAAMKRNKIQDIFKGKTLAMIFEK
CCEECCCEEEECCCCCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEC
SSTRTRVSFEAGMAQLGGNALFLSSKDMQLGRGETIADTAKVLSRYVDAIMIRTFEHEKV
CCCCEEEEHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHH
EELAKHAEIPVINGLTDLFHPCQALSDLLTIQEMKGTLKGLKAVYVGDGNNVAHSLLIGC
HHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHH
AKVGCDITVASPKGYEPNQDVVKLAETFAEESGAAVTITNDPVSAVQQADVIYSDVFTSM
HHHCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHC
GQEEEAEERMKIFAPYQVNGTLVSHAKPDYIFLHCLPAHREEEVTAEIIDGPNSAVFQQA
CCHHHHHHHHHEECCEEECCEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHHH
ENRLHVQKALLKALLS
HHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2117745; 8025667; 9384377; 9335269 [H]