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The map label for this gene is guaC

Identifier: 52784972

GI number: 52784972

Start: 1212575

End: 1213555

Strand: Reverse

Name: guaC

Synonym: BLi01200

Alternate gene names: 52784972

Gene position: 1213555-1212575 (Counterclockwise)

Preceding gene: 52784973

Following gene: 52784969

Centisome position: 28.74

GC content: 47.09

Gene sequence:

>981_bases
TTGGAAAATGTATTTGATTACGAAGATATTCAACTAATTCCTGCAAAGTGCATCGTCAAGAGCCGTTCTGAATGTGATAC
ATCCGTGCAGTTTGGCGGAAGGACATTTAAACTGCCTGTCGTGCCGGCCAATATGCAGACCATTATAGATGAAAAGCTGG
CTGTTTCATTAGCGGAAAACGGTTACTTTTATGTGATGCACCGCTTTGAGCCGGAAACACGGATCGATTTTATTAAAGAC
ATGAAGGCACGCGGTTTGTTTTCTTCAATCAGCGTCGGAGTCAAAGATGAAGAATACGCGTTTATCGAAGAGCTGACGAG
AGAGAATCTCACACCCGAGTACATCACGATCGATATCGCCCACGGTCACTCAAACGCCGTCATCAATATGATTCAGCACA
TTAAAAAACATCTTCCGGACAGCTTTGTGATCGCAGGAAATGTCGGAACGCCTGAAGCTGTTAGAGAGCTTGAAAACGCC
GGAGCCGACGCTACAAAAGTCGGCATCGGTCCCGGAAAAGTCTGCATTACAAAAATCAAAACCGGTTTTGGAACGGGCGG
ATGGCAGCTGGCTGCGCTGCGCTGGTGCGCAAAAGCGGCAAGCAAGCCGATCATTGCTGACGGCGGCATCCGCACCCACG
GAGACATCGCCAAATCGGTCAGATTCGGGGCGACAATGGTCATGATCGGTTCGCTGTTTGCCGGACACGAAGAATCGCCT
GGAGCAACGATCGAAAAAGACGGCAAGCTCTACAAAGAATATTTTGGTTCAGCTTCTGAGTACCAAAAAGGCGAAAAGAA
AAATGTCGAAGGCAAAAAAATGTACGTGGAGCATAAAGGCGCGATCATGGATACGCTGACAGAAATGGAACAGGATCTGC
AGTCGTCCATTTCTTATGCGGGAGGAAACAAGCTCGATGCGATCCGCAACGTAGACTATGTCATCGTTAAAAATTCTATT
TTCAATGGGGATCAATATTAA

Upstream 100 bases:

>100_bases
CATAAAAATTTGACATCGGGAACAAAAGGAAGTACATTATAAAAGGCGAACATTTTAAATAACTTATTAATAAATATTCG
TTTTTTTGGGGGTTTTTATA

Downstream 100 bases:

>100_bases
CGGTCACATCCTGTGACCGTGGCGGTGTTCATTCAGCCGCCGATCCTTCCTGACAGAATACAAAAGAGGCATCCTTTCCC
GGGATGCCTTTTTTGATGGT

Product: guanosine 5'-monophosphate oxidoreductase

Products: NA

Alternate protein names: Guanosine 5'-monophosphate oxidoreductase; Guanosine monophosphate reductase

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MENVFDYEDIQLIPAKCIVKSRSECDTSVQFGGRTFKLPVVPANMQTIIDEKLAVSLAENGYFYVMHRFEPETRIDFIKD
MKARGLFSSISVGVKDEEYAFIEELTRENLTPEYITIDIAHGHSNAVINMIQHIKKHLPDSFVIAGNVGTPEAVRELENA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESP
GATIEKDGKLYKEYFGSASEYQKGEKKNVEGKKMYVEHKGAIMDTLTEMEQDLQSSISYAGGNKLDAIRNVDYVIVKNSI
FNGDQY

Sequences:

>Translated_326_residues
MENVFDYEDIQLIPAKCIVKSRSECDTSVQFGGRTFKLPVVPANMQTIIDEKLAVSLAENGYFYVMHRFEPETRIDFIKD
MKARGLFSSISVGVKDEEYAFIEELTRENLTPEYITIDIAHGHSNAVINMIQHIKKHLPDSFVIAGNVGTPEAVRELENA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESP
GATIEKDGKLYKEYFGSASEYQKGEKKNVEGKKMYVEHKGAIMDTLTEMEQDLQSSISYAGGNKLDAIRNVDYVIVKNSI
FNGDQY
>Mature_326_residues
MENVFDYEDIQLIPAKCIVKSRSECDTSVQFGGRTFKLPVVPANMQTIIDEKLAVSLAENGYFYVMHRFEPETRIDFIKD
MKARGLFSSISVGVKDEEYAFIEELTRENLTPEYITIDIAHGHSNAVINMIQHIKKHLPDSFVIAGNVGTPEAVRELENA
GADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESP
GATIEKDGKLYKEYFGSASEYQKGEKKNVEGKKMYVEHKGAIMDTLTEMEQDLQSSISYAGGNKLDAIRNVDYVIVKNSI
FNGDQY

Specific function: Catalyzes the irreversible NADPH-dependent deamination of GMP to IMP. It functions in the conversion of nucleobase, nucleoside and nucleotide derivatives of G to A nucleotides, and in maintaining the intracellular balance of A and G nucleotides

COG id: COG0516

COG function: function code F; IMP dehydrogenase/GMP reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IMPDH/GMPR family. GuaC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI50541954, Length=338, Percent_Identity=34.6153846153846, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI50541952, Length=338, Percent_Identity=34.6153846153846, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI50541948, Length=338, Percent_Identity=34.6153846153846, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI50541956, Length=338, Percent_Identity=34.6153846153846, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI156104880, Length=338, Percent_Identity=30.1775147928994, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI66933016, Length=326, Percent_Identity=29.1411042944785, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI217035150, Length=245, Percent_Identity=31.4285714285714, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI217035148, Length=245, Percent_Identity=31.4285714285714, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI156616279, Length=245, Percent_Identity=31.4285714285714, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI217035146, Length=245, Percent_Identity=31.4285714285714, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI34328930, Length=245, Percent_Identity=31.4285714285714, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI217035152, Length=245, Percent_Identity=31.4285714285714, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI34328928, Length=245, Percent_Identity=31.4285714285714, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI1786293, Length=340, Percent_Identity=32.0588235294118, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1788855, Length=215, Percent_Identity=37.6744186046512, Blast_Score=109, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17560440, Length=321, Percent_Identity=32.0872274143302, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI71994385, Length=132, Percent_Identity=39.3939393939394, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71994389, Length=151, Percent_Identity=37.7483443708609, Blast_Score=88, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6323464, Length=211, Percent_Identity=32.7014218009479, Blast_Score=103, Evalue=5e-23,
Organism=Saccharomyces cerevisiae, GI6323585, Length=211, Percent_Identity=32.7014218009479, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322012, Length=253, Percent_Identity=30.4347826086957, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319352, Length=153, Percent_Identity=31.3725490196078, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24641071, Length=250, Percent_Identity=30.8, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24641073, Length=250, Percent_Identity=30.8, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI28571163, Length=250, Percent_Identity=30.8, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): GUAC_BACLD (Q65LF6)

Other databases:

- EMBL:   AE017333
- EMBL:   CP000002
- RefSeq:   YP_078400.1
- RefSeq:   YP_090801.1
- ProteinModelPortal:   Q65LF6
- SMR:   Q65LF6
- STRING:   Q65LF6
- EnsemblBacteria:   EBBACT00000054478
- EnsemblBacteria:   EBBACT00000060372
- GeneID:   3030188
- GeneID:   3099019
- GenomeReviews:   AE017333_GR
- GenomeReviews:   CP000002_GR
- KEGG:   bld:BLi01200
- KEGG:   bli:BL05105
- NMPDR:   fig|279010.5.peg.2281
- eggNOG:   COG0516
- GeneTree:   EBGT00050000000995
- HOGENOM:   HBG298985
- OMA:   PDYITID
- ProtClustDB:   PRK05458
- BioCyc:   BLIC279010-1:BLI01200-MONOMER
- BioCyc:   BLIC279010:BL05105-MONOMER
- HAMAP:   MF_01511
- InterPro:   IPR013785
- InterPro:   IPR005994
- InterPro:   IPR015875
- InterPro:   IPR001093
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF036500
- TIGRFAMs:   TIGR01306

Pfam domain/function: PF00478 IMPDH

EC number: =1.7.1.7

Molecular weight: Translated: 35893; Mature: 35893

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00487 IMP_DH_GMP_RED

Important sites: ACT_SITE 175-175

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENVFDYEDIQLIPAKCIVKSRSECDTSVQFGGRTFKLPVVPANMQTIIDEKLAVSLAEN
CCCCCCCCCEEEECHHHHHHCCCCCCCHHEECCEEEEEEEECCCHHHHHHHHHHEEEECC
GYFYVMHRFEPETRIDFIKDMKARGLFSSISVGVKDEEYAFIEELTRENLTPEYITIDIA
CEEEEEEECCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
HGHSNAVINMIQHIKKHLPDSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIK
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEEC
TGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESP
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GATIEKDGKLYKEYFGSASEYQKGEKKNVEGKKMYVEHKGAIMDTLTEMEQDLQSSISYA
CCCCCCCCHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHC
GGNKLDAIRNVDYVIVKNSIFNGDQY
CCCCHHHHHCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MENVFDYEDIQLIPAKCIVKSRSECDTSVQFGGRTFKLPVVPANMQTIIDEKLAVSLAEN
CCCCCCCCCEEEECHHHHHHCCCCCCCHHEECCEEEEEEEECCCHHHHHHHHHHEEEECC
GYFYVMHRFEPETRIDFIKDMKARGLFSSISVGVKDEEYAFIEELTRENLTPEYITIDIA
CEEEEEEECCCCHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
HGHSNAVINMIQHIKKHLPDSFVIAGNVGTPEAVRELENAGADATKVGIGPGKVCITKIK
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCEEECCCCCCEEEEEEC
TGFGTGGWQLAALRWCAKAASKPIIADGGIRTHGDIAKSVRFGATMVMIGSLFAGHEESP
CCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
GATIEKDGKLYKEYFGSASEYQKGEKKNVEGKKMYVEHKGAIMDTLTEMEQDLQSSISYA
CCCCCCCCHHHHHHCCCHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHC
GGNKLDAIRNVDYVIVKNSIFNGDQY
CCCCHHHHHCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA