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The map label for this gene is amtB [C]

Identifier: 52784947

GI number: 52784947

Start: 1183021

End: 1184400

Strand: Reverse

Name: amtB [C]

Synonym: BLi01175

Alternate gene names: 52784947

Gene position: 1184400-1183021 (Counterclockwise)

Preceding gene: 52784948

Following gene: 52784945

Centisome position: 28.05

GC content: 54.71

Gene sequence:

>1380_bases
ATGAAAACCAAGGTATCTGCCGTCCTGTTCCTCAGTTTTTTCCTCGCAGCATCAGCTCTGGCCGCCGAACCGACGGTGCA
ATCGGTCAATGACTCGGTCAACATGACATGGGTAGCCATCGGCACTCTGCTCGTCTTTTTTATGCACGCCGGTTTTGCGA
TGGTTGAATCAGGCTTTACAAGAGCTAAAAACACGTTAAACATTTTAATGAAAAACTTCCTGACGATCTCTATCGGCTCC
ATCCTTTATTTCTTCGCCGGATACGCGTTCATGTTCGGGGACAGCGCCGGCGGGTTCATCGGCACAAGCGGTTTTGCTCT
AGCGGGTGATCAGGATGTCGGATTTTTCGTCTTTCAGGCCGTGTTTGCCGCTACGTGCGCCACCATTATTTCCGGCGCTG
TCGCTGAACGGATGAAGCTTGGCAGCTACATGATCTTGACGCTGTTTATGACCGGTCTCATCTATCCGGTTGTCGGCCAT
TGGACTTGGGGGGAAGGCTGGCTGTACGACCTCGGATTTGTCGATTTTGCCGGATCTTCAATCGTTCACTTGACAGGCGC
TGCGGGCGCAGTTGCGGCTGTCATGTTTCTCGGGCCGCGGATCGGAAAATATACAAACGGCAAGGTCAACGCCATTCCAG
GGCACAGCATTCCGCTGGGCGCTCTCGGCGTCTTTATCCTGTGGTTCGGCTGGTTTGGGTTCAACGGCGGAAGCACGCTT
GCGGCTGATCCCGCCGCCGTCCCGCACGTCATCACCACAACCCTTTTAGCGGCATCGGGCGGAGTCGTCAGCTCGGCGCT
GTTCACTTATTTCCGCTACGGCAGGATCGATCCTTCCCTGACGCTGAACGGCGCGCTAGGCGGCCTTGTCGGAATTACCG
CGGGCGCGGACGGCGTTTCATTTACAGGCTCGATATTGATCGGATTCATCGCCGGTGTCATTTTAGTTGTCGGCGTTCAG
TTTATCGACCAGGTGCTGAAGCTTGACGATCCCGTCGGCGCCCTTGCCGTTCACGGTATTTGCGGTGTTTGGGGGACGCT
TGCAGTCGGGCTTTTCAATACATCGAGCGGTCTCTTTTACGGAGGAGGAGCCGCACAGCTTGGCATTCAAGCCGTCGGCA
TTTTGTCGATCGCGGCCTGGACCCTCGCTGCCACATACGCTGTCGCCGCGGCAATGAAAGCCCTCGCTCCGATCAGGGTA
TCAAAAGAGGAGGAAATTTCCGGACTAGATTATGCCGAACACGGTTCTTCCGCGTACGAGTATAAAGAAAGCTACATCGA
TCAGGACGATGTTGACGTAAGCTCCGCTTTCGGAACGGATCTTGTCTCACGGCTCAACAATTTAAAGCCTGGACCTTCTT
CCAAAACTGAACAGGTGTGA

Upstream 100 bases:

>100_bases
CCGAATCCGGACAGGGGAATCAGGAAAGGAAGCGATTGTCTAAAACGACCGATTCCCTCCTGCTCTCTTTTAGTCGATAC
TTTAAGAAAGGGTGATTCTT

Downstream 100 bases:

>100_bases
CTCTCATGCATCCGGCTCTCAGGCCGGCTTGCTGATCATAGAAATCGGCGGTGTTCAAAATAAACGCCGCCGTTTCTTTA
TAGCTTAGCTGCCAAAACGC

Product: hypothetical protein

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MKTKVSAVLFLSFFLAASALAAEPTVQSVNDSVNMTWVAIGTLLVFFMHAGFAMVESGFTRAKNTLNILMKNFLTISIGS
ILYFFAGYAFMFGDSAGGFIGTSGFALAGDQDVGFFVFQAVFAATCATIISGAVAERMKLGSYMILTLFMTGLIYPVVGH
WTWGEGWLYDLGFVDFAGSSIVHLTGAAGAVAAVMFLGPRIGKYTNGKVNAIPGHSIPLGALGVFILWFGWFGFNGGSTL
AADPAAVPHVITTTLLAASGGVVSSALFTYFRYGRIDPSLTLNGALGGLVGITAGADGVSFTGSILIGFIAGVILVVGVQ
FIDQVLKLDDPVGALAVHGICGVWGTLAVGLFNTSSGLFYGGGAAQLGIQAVGILSIAAWTLAATYAVAAAMKALAPIRV
SKEEEISGLDYAEHGSSAYEYKESYIDQDDVDVSSAFGTDLVSRLNNLKPGPSSKTEQV

Sequences:

>Translated_459_residues
MKTKVSAVLFLSFFLAASALAAEPTVQSVNDSVNMTWVAIGTLLVFFMHAGFAMVESGFTRAKNTLNILMKNFLTISIGS
ILYFFAGYAFMFGDSAGGFIGTSGFALAGDQDVGFFVFQAVFAATCATIISGAVAERMKLGSYMILTLFMTGLIYPVVGH
WTWGEGWLYDLGFVDFAGSSIVHLTGAAGAVAAVMFLGPRIGKYTNGKVNAIPGHSIPLGALGVFILWFGWFGFNGGSTL
AADPAAVPHVITTTLLAASGGVVSSALFTYFRYGRIDPSLTLNGALGGLVGITAGADGVSFTGSILIGFIAGVILVVGVQ
FIDQVLKLDDPVGALAVHGICGVWGTLAVGLFNTSSGLFYGGGAAQLGIQAVGILSIAAWTLAATYAVAAAMKALAPIRV
SKEEEISGLDYAEHGSSAYEYKESYIDQDDVDVSSAFGTDLVSRLNNLKPGPSSKTEQV
>Mature_459_residues
MKTKVSAVLFLSFFLAASALAAEPTVQSVNDSVNMTWVAIGTLLVFFMHAGFAMVESGFTRAKNTLNILMKNFLTISIGS
ILYFFAGYAFMFGDSAGGFIGTSGFALAGDQDVGFFVFQAVFAATCATIISGAVAERMKLGSYMILTLFMTGLIYPVVGH
WTWGEGWLYDLGFVDFAGSSIVHLTGAAGAVAAVMFLGPRIGKYTNGKVNAIPGHSIPLGALGVFILWFGWFGFNGGSTL
AADPAAVPHVITTTLLAASGGVVSSALFTYFRYGRIDPSLTLNGALGGLVGITAGADGVSFTGSILIGFIAGVILVVGVQ
FIDQVLKLDDPVGALAVHGICGVWGTLAVGLFNTSSGLFYGGGAAQLGIQAVGILSIAAWTLAATYAVAAAMKALAPIRV
SKEEEISGLDYAEHGSSAYEYKESYIDQDDVDVSSAFGTDLVSRLNNLKPGPSSKTEQV

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=411, Percent_Identity=30.4136253041363, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI17550180, Length=470, Percent_Identity=35.3191489361702, Blast_Score=241, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI17550182, Length=468, Percent_Identity=33.974358974359, Blast_Score=232, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI32564042, Length=419, Percent_Identity=32.6968973747017, Blast_Score=189, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI133931100, Length=457, Percent_Identity=29.3216630196937, Blast_Score=177, Evalue=9e-45,
Organism=Saccharomyces cerevisiae, GI6324187, Length=436, Percent_Identity=31.1926605504587, Blast_Score=184, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6325396, Length=424, Percent_Identity=29.7169811320755, Blast_Score=149, Evalue=8e-37,
Organism=Saccharomyces cerevisiae, GI6321559, Length=453, Percent_Identity=28.2560706401766, Blast_Score=147, Evalue=3e-36,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=38.2211538461538, Blast_Score=240, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 47705; Mature: 47705

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTKVSAVLFLSFFLAASALAAEPTVQSVNDSVNMTWVAIGTLLVFFMHAGFAMVESGFT
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCHH
RAKNTLNILMKNFLTISIGSILYFFAGYAFMFGDSAGGFIGTSGFALAGDQDVGFFVFQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEECCCCHHHHHHHH
VFAATCATIISGAVAERMKLGSYMILTLFMTGLIYPVVGHWTWGEGWLYDLGFVDFAGSS
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHCCCCC
IVHLTGAAGAVAAVMFLGPRIGKYTNGKVNAIPGHSIPLGALGVFILWFGWFGFNGGSTL
EEEEECCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AADPAAVPHVITTTLLAASGGVVSSALFTYFRYGRIDPSLTLNGALGGLVGITAGADGVS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHEEECCCCCCC
FTGSILIGFIAGVILVVGVQFIDQVLKLDDPVGALAVHGICGVWGTLAVGLFNTSSGLFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEE
GGGAAQLGIQAVGILSIAAWTLAATYAVAAAMKALAPIRVSKEEEISGLDYAEHGSSAYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCCHHHH
YKESYIDQDDVDVSSAFGTDLVSRLNNLKPGPSSKTEQV
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKTKVSAVLFLSFFLAASALAAEPTVQSVNDSVNMTWVAIGTLLVFFMHAGFAMVESGFT
CCCHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCHH
RAKNTLNILMKNFLTISIGSILYFFAGYAFMFGDSAGGFIGTSGFALAGDQDVGFFVFQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEEEECCCCHHHHHHHH
VFAATCATIISGAVAERMKLGSYMILTLFMTGLIYPVVGHWTWGEGWLYDLGFVDFAGSS
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHCCCCC
IVHLTGAAGAVAAVMFLGPRIGKYTNGKVNAIPGHSIPLGALGVFILWFGWFGFNGGSTL
EEEEECCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
AADPAAVPHVITTTLLAASGGVVSSALFTYFRYGRIDPSLTLNGALGGLVGITAGADGVS
CCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHEEECCCCCCC
FTGSILIGFIAGVILVVGVQFIDQVLKLDDPVGALAVHGICGVWGTLAVGLFNTSSGLFY
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCCCEEE
GGGAAQLGIQAVGILSIAAWTLAATYAVAAAMKALAPIRVSKEEEISGLDYAEHGSSAYE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCCHHHH
YKESYIDQDDVDVSSAFGTDLVSRLNNLKPGPSSKTEQV
HHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]