Click here to switch to the map view.

The map label for this gene is asnO [H]

Identifier: 52784946

GI number: 52784946

Start: 1181048

End: 1182889

Strand: Direct

Name: asnO [H]

Synonym: BLi01174

Alternate gene names: 52784946

Gene position: 1181048-1182889 (Clockwise)

Preceding gene: 52784944

Following gene: 52784950

Centisome position: 27.97

GC content: 48.86

Gene sequence:

>1842_bases
ATGTGTGGAATAACGGGGTGGGTCGATTTTAAGAAGCCGCTTGTCCGAGAGCAGTCAATCATGGAGAAGATGACTGATAC
GCTTTCGAAAAGAGGACCTGATGATACGAATATTTGGGGAAAGCGCCATGTTTTATTCGGACATAAACGCTTGGCAGTTG
TGGATCTGGAAGGCGGCAGACAGCCGATGACCCGCACACATCAAGGATATGATTATACGATTTGCTATAATGGCGAGCTC
TATAATACGGAGGATTTGCGAAAAGAGCTTAGGCGGCGGGGGCATCAATTCAAGAGCCACTCAGATACAGAGGTTTTGCT
TCATTCATACATGGAATGGAAAGAGGAATGCATCGACAGGCTGAACGGAATTTTTGCATTTGCGGTATGGGATGAAAAGC
GGGATCTTCTTTTTGCGGCACGCGACCGGCTTGGTGTTAAACCGTTCTTTTACACCGAACAGGGCTCATCATTTTTGTTC
GGATCTGAAATCAAAGCCATCCTCGCTCATCCTGAAGTGAAAGCAAAGGTAGACCGTTCAGGGCTGGCAGAAATTTTCGG
CCTCGGCCCGTCACGATCGCCGGGGAGCGGTGTTTTTAAAGGAGTAAAGGAGATCCGCCCGGGGCATGCGATGATTTTCT
CAAAAGACGGTTTAACGGTTTGGCGCTACTGGAATGTGAAAAGCGAGAAGCATACGGACAGCTTTGACGAAACGGTGGAA
AATGTGCGGTCGCTGTTTCAGGACGCCGTTACAAGGCAGCTTGTGTCTGATGTGCCGGTATGTACGTTTTTATCTGGAGG
GGTCGATTCGAGCGCCATTACCGCAACGGCCGCCAAGCATTTCGAGAGAGAAGGGAAGGCGCCCCTTCATACGTTTTCGA
TCGATTATGAAGACAATGAGCAATATTTTACGTCAAGCTCATTTCAGCCAAACGCCGACGGCCCGTGGATTAAAAAAATG
TCCGATGCATTCGGAACGGTTCATCACAATTGCGTGATATCCCAGGAGGAGCTGGCTGCCTATTTGGAAGAAGCCGTACT
GGTTCGGGATTTGCCGGGGATGGCTGATGTCGATTCTTCCCTGCTATGGTTCTGCCGCGAGATTAAAAAAGACTTTGTCG
TGAGCCTTTCAGGGGAATGCGCGGACGAAATTTTCGGCGGCTATCCTTGGTTCCATTCGGCAGAAGATGTGAAAGGTTTC
CCATGGATGAGATCAATGGAAGAACGGATCGGACTTCTGCAGGACGGCTGGCAGAAAAAGCTGAACCTCAGCGAATATGT
GAAGGCAAAATATGAGGAAACAGTTAAAGAAACACCGCTGCTGGAAGGAGAAACGGGTGCGGAGAAACGAAGGAGAGAGC
TGTTTTACTTAAATATGCTCTGGTTTATGACAACGCTCTTGGATCGAAAAGACAGAATGAGCATGGGTGCGAGCCTCGAG
GTCAGGGTTCCGTTTGCAGATCACAGGCTTGTCGAGTATGTCTGGAATATTCCATGGGATGTCAAAATGCATGGCAGCCG
TGAAAAAGGCATCTTCCGCAAGGCGCTGGAAGGCATTTTGCCTGATGAAGTGCTGTACCGCAAAAAAAGCCCATATCCGA
AAACGCACCACCCGGCCTATACGAAAGCCGTGATACGCTGGCTGGAGTCATTGCTGGAGCAAAAAGACTCGGCGCTCTAT
GCTTTCTTAGAAAAGAAAAAACTGGAGCGGCTGATTGAGACGGAAGGTGCATCATTTCAAGTTCCGTGGTACGGACAATT
GATGAAGGGACCGCAGCTGATCGCCCACCTTGCCCAAATCCATACTTGGTTTGAAAGTTATCGAATTGATATCGAGGAAT
AA

Upstream 100 bases:

>100_bases
ACAATTCAACTATTATTTTAAGGTGCATAAAATAGGTAAAGTGACAAAGGAAGTGAAGCCTAAAGCAGAGGAAGTACATT
AGAATTGGAGCTGATCGACA

Downstream 100 bases:

>100_bases
CATAAAACACCCGGCATATGCCGGGTGTTCTGCGTTTTGGCAGCTAAGCTATAAAGAAACGGCGGCGTTTATTTTGAACA
CCGCCGATTTCTATGATCAG

Product: AsnO

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MCGITGWVDFKKPLVREQSIMEKMTDTLSKRGPDDTNIWGKRHVLFGHKRLAVVDLEGGRQPMTRTHQGYDYTICYNGEL
YNTEDLRKELRRRGHQFKSHSDTEVLLHSYMEWKEECIDRLNGIFAFAVWDEKRDLLFAARDRLGVKPFFYTEQGSSFLF
GSEIKAILAHPEVKAKVDRSGLAEIFGLGPSRSPGSGVFKGVKEIRPGHAMIFSKDGLTVWRYWNVKSEKHTDSFDETVE
NVRSLFQDAVTRQLVSDVPVCTFLSGGVDSSAITATAAKHFEREGKAPLHTFSIDYEDNEQYFTSSSFQPNADGPWIKKM
SDAFGTVHHNCVISQEELAAYLEEAVLVRDLPGMADVDSSLLWFCREIKKDFVVSLSGECADEIFGGYPWFHSAEDVKGF
PWMRSMEERIGLLQDGWQKKLNLSEYVKAKYEETVKETPLLEGETGAEKRRRELFYLNMLWFMTTLLDRKDRMSMGASLE
VRVPFADHRLVEYVWNIPWDVKMHGSREKGIFRKALEGILPDEVLYRKKSPYPKTHHPAYTKAVIRWLESLLEQKDSALY
AFLEKKKLERLIETEGASFQVPWYGQLMKGPQLIAHLAQIHTWFESYRIDIEE

Sequences:

>Translated_613_residues
MCGITGWVDFKKPLVREQSIMEKMTDTLSKRGPDDTNIWGKRHVLFGHKRLAVVDLEGGRQPMTRTHQGYDYTICYNGEL
YNTEDLRKELRRRGHQFKSHSDTEVLLHSYMEWKEECIDRLNGIFAFAVWDEKRDLLFAARDRLGVKPFFYTEQGSSFLF
GSEIKAILAHPEVKAKVDRSGLAEIFGLGPSRSPGSGVFKGVKEIRPGHAMIFSKDGLTVWRYWNVKSEKHTDSFDETVE
NVRSLFQDAVTRQLVSDVPVCTFLSGGVDSSAITATAAKHFEREGKAPLHTFSIDYEDNEQYFTSSSFQPNADGPWIKKM
SDAFGTVHHNCVISQEELAAYLEEAVLVRDLPGMADVDSSLLWFCREIKKDFVVSLSGECADEIFGGYPWFHSAEDVKGF
PWMRSMEERIGLLQDGWQKKLNLSEYVKAKYEETVKETPLLEGETGAEKRRRELFYLNMLWFMTTLLDRKDRMSMGASLE
VRVPFADHRLVEYVWNIPWDVKMHGSREKGIFRKALEGILPDEVLYRKKSPYPKTHHPAYTKAVIRWLESLLEQKDSALY
AFLEKKKLERLIETEGASFQVPWYGQLMKGPQLIAHLAQIHTWFESYRIDIEE
>Mature_613_residues
MCGITGWVDFKKPLVREQSIMEKMTDTLSKRGPDDTNIWGKRHVLFGHKRLAVVDLEGGRQPMTRTHQGYDYTICYNGEL
YNTEDLRKELRRRGHQFKSHSDTEVLLHSYMEWKEECIDRLNGIFAFAVWDEKRDLLFAARDRLGVKPFFYTEQGSSFLF
GSEIKAILAHPEVKAKVDRSGLAEIFGLGPSRSPGSGVFKGVKEIRPGHAMIFSKDGLTVWRYWNVKSEKHTDSFDETVE
NVRSLFQDAVTRQLVSDVPVCTFLSGGVDSSAITATAAKHFEREGKAPLHTFSIDYEDNEQYFTSSSFQPNADGPWIKKM
SDAFGTVHHNCVISQEELAAYLEEAVLVRDLPGMADVDSSLLWFCREIKKDFVVSLSGECADEIFGGYPWFHSAEDVKGF
PWMRSMEERIGLLQDGWQKKLNLSEYVKAKYEETVKETPLLEGETGAEKRRRELFYLNMLWFMTTLLDRKDRMSMGASLE
VRVPFADHRLVEYVWNIPWDVKMHGSREKGIFRKALEGILPDEVLYRKKSPYPKTHHPAYTKAVIRWLESLLEQKDSALY
AFLEKKKLERLIETEGASFQVPWYGQLMKGPQLIAHLAQIHTWFESYRIDIEE

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=413, Percent_Identity=31.2348668280872, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI168229252, Length=413, Percent_Identity=31.2348668280872, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI168229250, Length=413, Percent_Identity=31.2348668280872, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI296010848, Length=386, Percent_Identity=31.6062176165803, Blast_Score=145, Evalue=2e-34,
Organism=Homo sapiens, GI296010852, Length=315, Percent_Identity=30.7936507936508, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI296010850, Length=315, Percent_Identity=30.7936507936508, Blast_Score=110, Evalue=4e-24,
Organism=Escherichia coli, GI1786889, Length=556, Percent_Identity=26.978417266187, Blast_Score=173, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI71993933, Length=531, Percent_Identity=27.4952919020716, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25147557, Length=531, Percent_Identity=27.4952919020716, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17560178, Length=414, Percent_Identity=27.0531400966184, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI25147560, Length=314, Percent_Identity=29.2993630573248, Blast_Score=96, Evalue=7e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=566, Percent_Identity=25.0883392226148, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6325403, Length=570, Percent_Identity=25.7894736842105, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI45553209, Length=521, Percent_Identity=27.063339731286, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70745; Mature: 70745

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWVDFKKPLVREQSIMEKMTDTLSKRGPDDTNIWGKRHVLFGHKRLAVVDLEGGR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCC
QPMTRTHQGYDYTICYNGELYNTEDLRKELRRRGHQFKSHSDTEVLLHSYMEWKEECIDR
CCCCCCCCCCEEEEEECCCEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNGIFAFAVWDEKRDLLFAARDRLGVKPFFYTEQGSSFLFGSEIKAILAHPEVKAKVDRS
HCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCEEEHHHHHHHHCCCCHHHHCCHH
GLAEIFGLGPSRSPGSGVFKGVKEIRPGHAMIFSKDGLTVWRYWNVKSEKHTDSFDETVE
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHHH
NVRSLFQDAVTRQLVSDVPVCTFLSGGVDSSAITATAAKHFEREGKAPLHTFSIDYEDNE
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
QYFTSSSFQPNADGPWIKKMSDAFGTVHHNCVISQEELAAYLEEAVLVRDLPGMADVDSS
CEEECCCCCCCCCCHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWFCREIKKDFVVSLSGECADEIFGGYPWFHSAEDVKGFPWMRSMEERIGLLQDGWQKK
HHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHC
LNLSEYVKAKYEETVKETPLLEGETGAEKRRRELFYLNMLWFMTTLLDRKDRMSMGASLE
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEE
VRVPFADHRLVEYVWNIPWDVKMHGSREKGIFRKALEGILPDEVLYRKKSPYPKTHHPAY
EECCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCHH
TKAVIRWLESLLEQKDSALYAFLEKKKLERLIETEGASFQVPWYGQLMKGPQLIAHLAQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHHHHHHHH
HTWFESYRIDIEE
HHHHHHHCCCCCC
>Mature Secondary Structure
MCGITGWVDFKKPLVREQSIMEKMTDTLSKRGPDDTNIWGKRHVLFGHKRLAVVDLEGGR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCC
QPMTRTHQGYDYTICYNGELYNTEDLRKELRRRGHQFKSHSDTEVLLHSYMEWKEECIDR
CCCCCCCCCCEEEEEECCCEECHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNGIFAFAVWDEKRDLLFAARDRLGVKPFFYTEQGSSFLFGSEIKAILAHPEVKAKVDRS
HCCEEEEEEECCCCHHHHHHHHHCCCCCEEEECCCCCEEEHHHHHHHHCCCCHHHHCCHH
GLAEIFGLGPSRSPGSGVFKGVKEIRPGHAMIFSKDGLTVWRYWNVKSEKHTDSFDETVE
HHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHHHH
NVRSLFQDAVTRQLVSDVPVCTFLSGGVDSSAITATAAKHFEREGKAPLHTFSIDYEDNE
HHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCC
QYFTSSSFQPNADGPWIKKMSDAFGTVHHNCVISQEELAAYLEEAVLVRDLPGMADVDSS
CEEECCCCCCCCCCHHHHHHHHHHHHHHHCEEECHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWFCREIKKDFVVSLSGECADEIFGGYPWFHSAEDVKGFPWMRSMEERIGLLQDGWQKK
HHHHHHHHHHHHEEECCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHC
LNLSEYVKAKYEETVKETPLLEGETGAEKRRRELFYLNMLWFMTTLLDRKDRMSMGASLE
CCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEE
VRVPFADHRLVEYVWNIPWDVKMHGSREKGIFRKALEGILPDEVLYRKKSPYPKTHHPAY
EECCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCHH
TKAVIRWLESLLEQKDSALYAFLEKKKLERLIETEGASFQVPWYGQLMKGPQLIAHLAQI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHCCHHHHHHHHHH
HTWFESYRIDIEE
HHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]