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The map label for this gene is sbcD [H]

Identifier: 52784930

GI number: 52784930

Start: 1168211

End: 1169392

Strand: Direct

Name: sbcD [H]

Synonym: BLi01158

Alternate gene names: 52784930

Gene position: 1168211-1169392 (Clockwise)

Preceding gene: 52784929

Following gene: 52784931

Centisome position: 27.67

GC content: 53.38

Gene sequence:

>1182_bases
ATGCGAATTTTACATACAGCGGACTGGCATTTGGGAAAAACGCTTGAAGGGAGAAGCCGACTGAAAGAACAGGAGGATTT
TCTTGAAGAGCTCGCAAGGATCGTAAAAGATGAAAAGATCGACGCTGTTATCATGGCCGGAGACGCTTTTGACACAGTGA
ACCCGCCTGCACTGGCTGAACAGCTTTTTTATGAAAGCCTCTCAGCCTTGTCTGACAAAGGAAACCGTCCGGTCGTCGTG
ATTGCCGGCAATCATGACAATCCGGACCGCCTTTCCGCGGCCTCTCCGTTAACGACAGACCACGGCATCCACTTGATCGG
GTACCCGAGGACTGCGCCGGTTGACATAGAAGTCGCTTCTAGTGGGGAGATCTTGTCAGTCGCGGCGCTCGCCTATCCTT
CCGAGTCCAGACTGAATGAAGTGCTGTCGGAGACATTTGATGAAAAGCTGCTGCGCGACCAGTATGATGAGAAAATCAAG
CAGACCTTTCAGCGTATGTGCAGCAAGGCGAGAAAGGACGCGGTGCAGATTGCGGCAAGCCATATCTATGTGGCCGGCGG
CAGCCAGACGGATTCCGAGCGGCCGATCGAAGTCGGAGGCGCATATACGGTCGCTGCGGAAAGCCTGCCGGAAAACGCCG
CATATGTGGCGCTCGGGCATCTTCATCGCCCGCAGACGATCAAAAGGGCCCGCACGCTTGCCAGATATTCAGGCTCACCG
CTTGCCTACAGCTTTTCCGAAGCGGGATACGCAAAATCAGTCACCATTGTCGAAGCCGCACCCGGTCAAACGGCATCATG
GGAAGAGGTCCATTTGACGAGTGGAAAACCGCTCGTCAAATGGAAGGCGGAAAAAGGGATGTCCCAAGTGTACAGCTGGC
TGGAGGAAGAGCGGGATAAAAACGCGTGGATCGATCTTGAAATCCATCTGACAGACCAGCTGTCGATTGAAGAGATTCAC
CGATTGAGAAAAGCGCGTCAAGGCATCATCCATATACGTCCGGTTTTTGAGGAAGCCGCATCGGCTGAAGAGAAGTCCCG
GTCCAGAAACGTTCCGATTGAAGAACGGTTTAAAGCGTTTTATGAAAGACAAACCGGCGGAGCGGTTCCCGATGAGGAAA
CCGTCAAGCTGTTTCTGGAACTCGCCTCAGGATCGGAACAGGAAGAGGGGGATGAAACATGA

Upstream 100 bases:

>100_bases
TACTTTTTTGACGGAGGACACGTGCTCTTATTTTAATATGAAGCAGCTTCCCCCTGACGATTTCGGGGGGAGCTTTAATG
AAAAGAGAGGTGAGGCGCAC

Downstream 100 bases:

>100_bases
AGCCGATTTCTCTGTCTATTAAGGGCCTGCACAGTTTCAGGCAGGAGCAGGTGATCGATTTTGAGCGCCTGTGCGATGCC
GGTGTATTCGGCATCTTCGG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MRILHTADWHLGKTLEGRSRLKEQEDFLEELARIVKDEKIDAVIMAGDAFDTVNPPALAEQLFYESLSALSDKGNRPVVV
IAGNHDNPDRLSAASPLTTDHGIHLIGYPRTAPVDIEVASSGEILSVAALAYPSESRLNEVLSETFDEKLLRDQYDEKIK
QTFQRMCSKARKDAVQIAASHIYVAGGSQTDSERPIEVGGAYTVAAESLPENAAYVALGHLHRPQTIKRARTLARYSGSP
LAYSFSEAGYAKSVTIVEAAPGQTASWEEVHLTSGKPLVKWKAEKGMSQVYSWLEEERDKNAWIDLEIHLTDQLSIEEIH
RLRKARQGIIHIRPVFEEAASAEEKSRSRNVPIEERFKAFYERQTGGAVPDEETVKLFLELASGSEQEEGDET

Sequences:

>Translated_393_residues
MRILHTADWHLGKTLEGRSRLKEQEDFLEELARIVKDEKIDAVIMAGDAFDTVNPPALAEQLFYESLSALSDKGNRPVVV
IAGNHDNPDRLSAASPLTTDHGIHLIGYPRTAPVDIEVASSGEILSVAALAYPSESRLNEVLSETFDEKLLRDQYDEKIK
QTFQRMCSKARKDAVQIAASHIYVAGGSQTDSERPIEVGGAYTVAAESLPENAAYVALGHLHRPQTIKRARTLARYSGSP
LAYSFSEAGYAKSVTIVEAAPGQTASWEEVHLTSGKPLVKWKAEKGMSQVYSWLEEERDKNAWIDLEIHLTDQLSIEEIH
RLRKARQGIIHIRPVFEEAASAEEKSRSRNVPIEERFKAFYERQTGGAVPDEETVKLFLELASGSEQEEGDET
>Mature_393_residues
MRILHTADWHLGKTLEGRSRLKEQEDFLEELARIVKDEKIDAVIMAGDAFDTVNPPALAEQLFYESLSALSDKGNRPVVV
IAGNHDNPDRLSAASPLTTDHGIHLIGYPRTAPVDIEVASSGEILSVAALAYPSESRLNEVLSETFDEKLLRDQYDEKIK
QTFQRMCSKARKDAVQIAASHIYVAGGSQTDSERPIEVGGAYTVAAESLPENAAYVALGHLHRPQTIKRARTLARYSGSP
LAYSFSEAGYAKSVTIVEAAPGQTASWEEVHLTSGKPLVKWKAEKGMSQVYSWLEEERDKNAWIDLEIHLTDQLSIEEIH
RLRKARQGIIHIRPVFEEAASAEEKSRSRNVPIEERFKAFYERQTGGAVPDEETVKLFLELASGSEQEEGDET

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=346, Percent_Identity=30.635838150289, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 43681; Mature: 43681

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTADWHLGKTLEGRSRLKEQEDFLEELARIVKDEKIDAVIMAGDAFDTVNPPALAE
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
QLFYESLSALSDKGNRPVVVIAGNHDNPDRLSAASPLTTDHGIHLIGYPRTAPVDIEVAS
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCEEEEECCCCCCEEEEECC
SGEILSVAALAYPSESRLNEVLSETFDEKLLRDQYDEKIKQTFQRMCSKARKDAVQIAAS
CCCEEEEHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIYVAGGSQTDSERPIEVGGAYTVAAESLPENAAYVALGHLHRPQTIKRARTLARYSGSP
EEEEECCCCCCCCCCEECCCEEEEEHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
LAYSFSEAGYAKSVTIVEAAPGQTASWEEVHLTSGKPLVKWKAEKGMSQVYSWLEEERDK
CEEECCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEHHHCCHHHHHHHHHHHCCC
NAWIDLEIHLTDQLSIEEIHRLRKARQGIIHIRPVFEEAASAEEKSRSRNVPIEERFKAF
CCEEEEEEEECCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHH
YERQTGGAVPDEETVKLFLELASGSEQEEGDET
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MRILHTADWHLGKTLEGRSRLKEQEDFLEELARIVKDEKIDAVIMAGDAFDTVNPPALAE
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHH
QLFYESLSALSDKGNRPVVVIAGNHDNPDRLSAASPLTTDHGIHLIGYPRTAPVDIEVAS
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHCCCCCCCCCCCEEEEECCCCCCEEEEECC
SGEILSVAALAYPSESRLNEVLSETFDEKLLRDQYDEKIKQTFQRMCSKARKDAVQIAAS
CCCEEEEHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HIYVAGGSQTDSERPIEVGGAYTVAAESLPENAAYVALGHLHRPQTIKRARTLARYSGSP
EEEEECCCCCCCCCCEECCCEEEEEHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
LAYSFSEAGYAKSVTIVEAAPGQTASWEEVHLTSGKPLVKWKAEKGMSQVYSWLEEERDK
CEEECCCCCCCEEEEEEEECCCCCCCCCEEEECCCCCEEEEHHHCCHHHHHHHHHHHCCC
NAWIDLEIHLTDQLSIEEIHRLRKARQGIIHIRPVFEEAASAEEKSRSRNVPIEERFKAF
CCEEEEEEEECCCCCHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHHH
YERQTGGAVPDEETVKLFLELASGSEQEEGDET
HHHCCCCCCCCHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]