Click here to switch to the map view.

The map label for this gene is ndhF [H]

Identifier: 52784644

GI number: 52784644

Start: 864449

End: 865981

Strand: Reverse

Name: ndhF [H]

Synonym: BLi00846

Alternate gene names: 52784644

Gene position: 865981-864449 (Counterclockwise)

Preceding gene: 52784645

Following gene: 52784643

Centisome position: 20.51

GC content: 53.88

Gene sequence:

>1533_bases
ATGTTTGATATGCTAGATTCTTTATTTTGGCTGAAAATATTTTTCGCGTCGCTTGCGCTTTGTTTGTTCAGCGCACTGCT
CGTCCTGAATCGAAAGGTTCCATTGGCATATGTACGGATTCATGCCAAAATCATCGCCCTGCCTCCGCTTGCCGCGCTGG
CAGCATTGATAACGGCAAATCCCGGGCAGGCTGAAGCTTCTTGGCGCTTGGATTCGCTTGCTTGGCTGATCGCCTTGTTT
GTCTTGACTATCGGCTTCATCGTCCAGCGCTACTCTGTCCGGTATTTATTCGGCGACCGCTCCTACCGTAAATTTTTTGT
GCTGCTGACGCTTACGACAGGAGGAGCTTCATGCGCCTGGCTGAACGATGATCTTCGCTGGCTCCTTTTATGCTGGGGGA
TCACGCTGCTCGGCTTGACTGCGCTAATCGGCTTAAACAGCGCGTGGCAGGCGGCGAAAAATGCCGCTTTGTATGCGGGC
CGGATCTTTGCGCTCAGCTGGCTGGCGATAGCAGCTGCCGCTCTTTGGCTGTCTCTTTCGACGGGACACTGGCGATTATC
ATCCGCCATAGCGGAAGAAAGCCTTGTACAGCTCAGCTCATGGGAGAAAACCGGGATCGGCCTCCTCGTCATTTTAACGG
TGATGATTCCTGCGGCACAGTGGCCGTTTCATCGTTGGCTGCTCGATTCAGCAATCGCTCCGACTCCCGTTTCCGCAGTC
ATGCACGCCGGACTTGTAAATGTAGGCGGCGTCATGCTGACTCGTTTTGCACCGATGTTCAGCGGTGATGTACTGCAGCT
TATTCTGCTTGTACCCGCCGTCCTTTCCGTCTTGCTCGGAACGGGCATGATGATCGTCCAAACGGACTACAAGCGGCAGC
TCGCAGCTTCAACGGTCGCGCAGATGGGTTTTATGCTCGTCCAATGCGCATTAGGGGCCTATATTGCGGCTGTTATTCAC
CTCATTTTGCACGGCCTGTTCAAAGCCGCCCTTTTCCTGCAAGCCGGATCAGCGGTGCGCCGTTATGAATCGTTCGCATC
TAAACCGGGCAGACCGGCTCTTTTTTGGAACATGATAGGCGGCGTCTTTGCTCTTGCTCTCGTCATCGGAATCTGGGCCT
CAGCTACGGACGATGGCTATCAATTGATCAGCGCCCTTGTTTTAGGCTGGGCACTGTTCATTGCTTGGAGGCAGCTTGTC
ACAGCCAATTACGGACGCGTCACACGAATTGCCGGACTTTTCATTTTAACAGGGGCTTCACTCGCGTTCGCCATCATTCA
TTCCGTTTTACACGGGCTCCTGAAGGATGCTGTTGTACAGGGGGCTGAGCCGCCTGCAATGGCGGCCGTTTTGACGGCAG
TCGTTTTGTTAGCCGGCAGTGCGGCCGGCGCGTTGATCGTTCGGCAGCGCTTTGCATGGTCCGGCGCACTTTATTTACGT
CTTGTGAAAACGGGTGAACCCCATTTCGAATCGGTCGAAGAACGGCCGAATTATCTTGACCAATATCTTACTCAGGGAGG
TCGCCAATCATGA

Upstream 100 bases:

>100_bases
GGCCAGTCCTGAATATACCGGCTTTTTTTACGTTTTCAGAAAGGAAGGCAAGATATGAAACAGTATGTTCTATTCCATCA
CCCCGACGGAGGGATGCCGG

Downstream 100 bases:

>100_bases
AAGCAGCACCCACATTGTCTAAACAATTGATATTCCAATATGAACATCAGACTGCGGACTTTAATATGGATGAGTTGGTT
CAATCAGCAGCACGGGTCAT

Product: NADH dehydrogenase subunit 5

Products: NA

Alternate protein names: NADH dehydrogenase subunit 5 [H]

Number of amino acids: Translated: 510; Mature: 510

Protein sequence:

>510_residues
MFDMLDSLFWLKIFFASLALCLFSALLVLNRKVPLAYVRIHAKIIALPPLAALAALITANPGQAEASWRLDSLAWLIALF
VLTIGFIVQRYSVRYLFGDRSYRKFFVLLTLTTGGASCAWLNDDLRWLLLCWGITLLGLTALIGLNSAWQAAKNAALYAG
RIFALSWLAIAAAALWLSLSTGHWRLSSAIAEESLVQLSSWEKTGIGLLVILTVMIPAAQWPFHRWLLDSAIAPTPVSAV
MHAGLVNVGGVMLTRFAPMFSGDVLQLILLVPAVLSVLLGTGMMIVQTDYKRQLAASTVAQMGFMLVQCALGAYIAAVIH
LILHGLFKAALFLQAGSAVRRYESFASKPGRPALFWNMIGGVFALALVIGIWASATDDGYQLISALVLGWALFIAWRQLV
TANYGRVTRIAGLFILTGASLAFAIIHSVLHGLLKDAVVQGAEPPAMAAVLTAVVLLAGSAAGALIVRQRFAWSGALYLR
LVKTGEPHFESVEERPNYLDQYLTQGGRQS

Sequences:

>Translated_510_residues
MFDMLDSLFWLKIFFASLALCLFSALLVLNRKVPLAYVRIHAKIIALPPLAALAALITANPGQAEASWRLDSLAWLIALF
VLTIGFIVQRYSVRYLFGDRSYRKFFVLLTLTTGGASCAWLNDDLRWLLLCWGITLLGLTALIGLNSAWQAAKNAALYAG
RIFALSWLAIAAAALWLSLSTGHWRLSSAIAEESLVQLSSWEKTGIGLLVILTVMIPAAQWPFHRWLLDSAIAPTPVSAV
MHAGLVNVGGVMLTRFAPMFSGDVLQLILLVPAVLSVLLGTGMMIVQTDYKRQLAASTVAQMGFMLVQCALGAYIAAVIH
LILHGLFKAALFLQAGSAVRRYESFASKPGRPALFWNMIGGVFALALVIGIWASATDDGYQLISALVLGWALFIAWRQLV
TANYGRVTRIAGLFILTGASLAFAIIHSVLHGLLKDAVVQGAEPPAMAAVLTAVVLLAGSAAGALIVRQRFAWSGALYLR
LVKTGEPHFESVEERPNYLDQYLTQGGRQS
>Mature_510_residues
MFDMLDSLFWLKIFFASLALCLFSALLVLNRKVPLAYVRIHAKIIALPPLAALAALITANPGQAEASWRLDSLAWLIALF
VLTIGFIVQRYSVRYLFGDRSYRKFFVLLTLTTGGASCAWLNDDLRWLLLCWGITLLGLTALIGLNSAWQAAKNAALYAG
RIFALSWLAIAAAALWLSLSTGHWRLSSAIAEESLVQLSSWEKTGIGLLVILTVMIPAAQWPFHRWLLDSAIAPTPVSAV
MHAGLVNVGGVMLTRFAPMFSGDVLQLILLVPAVLSVLLGTGMMIVQTDYKRQLAASTVAQMGFMLVQCALGAYIAAVIH
LILHGLFKAALFLQAGSAVRRYESFASKPGRPALFWNMIGGVFALALVIGIWASATDDGYQLISALVLGWALFIAWRQLV
TANYGRVTRIAGLFILTGASLAFAIIHSVLHGLLKDAVVQGAEPPAMAAVLTAVVLLAGSAAGALIVRQRFAWSGALYLR
LVKTGEPHFESVEERPNYLDQYLTQGGRQS

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=129, Percent_Identity=31.7829457364341, Blast_Score=73, Evalue=5e-13,
Organism=Escherichia coli, GI1788614, Length=284, Percent_Identity=31.6901408450704, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788831, Length=130, Percent_Identity=34.6153846153846, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1788829, Length=293, Percent_Identity=29.3515358361775, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55254; Mature: 55254

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDMLDSLFWLKIFFASLALCLFSALLVLNRKVPLAYVRIHAKIIALPPLAALAALITAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCHHHHHHHHHHCC
PGQAEASWRLDSLAWLIALFVLTIGFIVQRYSVRYLFGDRSYRKFFVLLTLTTGGASCAW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEECCCCEEEE
LNDDLRWLLLCWGITLLGLTALIGLNSAWQAAKNAALYAGRIFALSWLAIAAAALWLSLS
ECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGHWRLSSAIAEESLVQLSSWEKTGIGLLVILTVMIPAAQWPFHRWLLDSAIAPTPVSAV
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
MHAGLVNVGGVMLTRFAPMFSGDVLQLILLVPAVLSVLLGTGMMIVQTDYKRQLAASTVA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHH
QMGFMLVQCALGAYIAAVIHLILHGLFKAALFLQAGSAVRRYESFASKPGRPALFWNMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
GVFALALVIGIWASATDDGYQLISALVLGWALFIAWRQLVTANYGRVTRIAGLFILTGAS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LAFAIIHSVLHGLLKDAVVQGAEPPAMAAVLTAVVLLAGSAAGALIVRQRFAWSGALYLR
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHEE
LVKTGEPHFESVEERPNYLDQYLTQGGRQS
EECCCCCHHHHHHHCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFDMLDSLFWLKIFFASLALCLFSALLVLNRKVPLAYVRIHAKIIALPPLAALAALITAN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCHHHHHHHHHHCC
PGQAEASWRLDSLAWLIALFVLTIGFIVQRYSVRYLFGDRSYRKFFVLLTLTTGGASCAW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEEEEEEECCCCEEEE
LNDDLRWLLLCWGITLLGLTALIGLNSAWQAAKNAALYAGRIFALSWLAIAAAALWLSLS
ECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGHWRLSSAIAEESLVQLSSWEKTGIGLLVILTVMIPAAQWPFHRWLLDSAIAPTPVSAV
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
MHAGLVNVGGVMLTRFAPMFSGDVLQLILLVPAVLSVLLGTGMMIVQTDYKRQLAASTVA
HHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHH
QMGFMLVQCALGAYIAAVIHLILHGLFKAALFLQAGSAVRRYESFASKPGRPALFWNMIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHH
GVFALALVIGIWASATDDGYQLISALVLGWALFIAWRQLVTANYGRVTRIAGLFILTGAS
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LAFAIIHSVLHGLLKDAVVQGAEPPAMAAVLTAVVLLAGSAAGALIVRQRFAWSGALYLR
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHEE
LVKTGEPHFESVEERPNYLDQYLTQGGRQS
EECCCCCHHHHHHHCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]