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The map label for this gene is yefA [H]

Identifier: 52784541

GI number: 52784541

Start: 747785

End: 749173

Strand: Direct

Name: yefA [H]

Synonym: BLi00742

Alternate gene names: 52784541

Gene position: 747785-749173 (Clockwise)

Preceding gene: 52784540

Following gene: 52784542

Centisome position: 17.71

GC content: 52.99

Gene sequence:

>1389_bases
ATGAAACAAAAAGCCCCCGTTCAAAAAAATGAATATTACAATGTCGTATTTGAAGATTTAACACACGAAGGAGCCGGCGT
CGCCAAAGTCGACGGCTTCCCGATTTTCGTGCCGAACGCGCTGCCTGACGAAAAGGCGCAGATTAAAGTGACCCGGGTCA
AAAAAGGCTTCGCCTTCGGCCGCCTCATCGACTTGAAAGAAGAAAGCCGCTTTCGCACGGACGCGCCGTGCCCGATCTAC
AAGCAGTGCGGCGGCTGCCAGCTGCAGCACATCAATTACGAAGGCCAGCTTCTCTACAAGCAGAAGCAAGTCAAAGACGT
GCTCGAGCGAATCGGCAAGCTCGATTTAAGCCGGATCACCGTCCACCCGACCCTCGGCATGGAAGACCCGTGGAACTACC
GAAACAAAGCGCAGGTCCCTGTCGGCGAAAGAGAAGGCGGCCTGATCGCAGGCTTCTACCAGCAGCGCAGCCATGAGATC
ATCGACATGAACGAATGCCTGATCCAGCAATCGGAAAACGACCGCGTCGTTCAAGCGATTAAAGAGATCTGCGGCAAATA
CGGCATCAAAGCGTACAACGAAGAACGCCACAAAGGCTGGCTCAGACACATCATGGTCAGATACGGCGCCGTCACAGGCG
AAATGATGGTCGTCTTCATCACAAGGACAAACGACTTCCCGCACAAAGCGAAAATCGTCGAAGAGATCACCGCGGCATTC
CCGCACGTCAAATCGATCGTCCAAAACATCAACCCGAAAAAAACAAACGTCATCTTCGGCGACGAAACAACGGTCATCTG
GGGAGAAGAATACATTTACGACACAATCGGCGGCATCAAATTCGCCATCTCCGCTAGATCGTTCTACCAAGTCAACCCTG
AGCAAACGAAAGTGCTCTACGACAAAGCGCTTGAATACGCCGAATTGAACGGCGAAGAGACCGTCATCGACGCCTACTGC
GGCATCGGCACGATTTCGCTGTTTTTAGCGAAGCAGGCGAAGAAAGTGTACGGCGTTGAGATTGTGCCGGAAGCGATTGA
GGATGCGAAGCGGAATGCTGAGTTGAATGGCATTGAGAACGCTGAGTTTGAAGTTGGCGAAGCTGAGGTCGTGATTCCGA
AGTGGTATGAGGAAGGCATTGCGGCTGACACGCTAGTGGTTGACCCGCCGCGCAAAGGCTGCGATGAGGCTTTGCTGCGG
ACGATTATTGAGATGAAGCCGAAGCGGGTTGTGTATGTGTCTTGCAACCCGGGAACCCTGGCGCGCGATTTGCGGGTGCT
GGAGGATGGCGGGTATGTGACGCGTGAGGTACAGCCTGTTGATATGTTTCCGCATACGAGCCATGTGGAGTGTGTGACGA
AGTTGGTTTTAAAAGGTGCAGTTGATTAA

Upstream 100 bases:

>100_bases
CCATATCGACGTCGTCATGTCAAAAGAAAAAGCGAGAAAATTGGATGATTGAACTTGGTCTAAAGAGCTGGCCAAGTTTT
TCTTATAGCAGGTGAAAAAG

Downstream 100 bases:

>100_bases
ACTGTACCTAATAGTTTAAAAACTTTGCAGATGACTTCTTTTCTTCTTCAAAGACTTAATCTTTTTGATGTTGCTCTTTG
ATAACTTTTTTGAAGGCCTG

Product: YefA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKQKAPVQKNEYYNVVFEDLTHEGAGVAKVDGFPIFVPNALPDEKAQIKVTRVKKGFAFGRLIDLKEESRFRTDAPCPIY
KQCGGCQLQHINYEGQLLYKQKQVKDVLERIGKLDLSRITVHPTLGMEDPWNYRNKAQVPVGEREGGLIAGFYQQRSHEI
IDMNECLIQQSENDRVVQAIKEICGKYGIKAYNEERHKGWLRHIMVRYGAVTGEMMVVFITRTNDFPHKAKIVEEITAAF
PHVKSIVQNINPKKTNVIFGDETTVIWGEEYIYDTIGGIKFAISARSFYQVNPEQTKVLYDKALEYAELNGEETVIDAYC
GIGTISLFLAKQAKKVYGVEIVPEAIEDAKRNAELNGIENAEFEVGEAEVVIPKWYEEGIAADTLVVDPPRKGCDEALLR
TIIEMKPKRVVYVSCNPGTLARDLRVLEDGGYVTREVQPVDMFPHTSHVECVTKLVLKGAVD

Sequences:

>Translated_462_residues
MKQKAPVQKNEYYNVVFEDLTHEGAGVAKVDGFPIFVPNALPDEKAQIKVTRVKKGFAFGRLIDLKEESRFRTDAPCPIY
KQCGGCQLQHINYEGQLLYKQKQVKDVLERIGKLDLSRITVHPTLGMEDPWNYRNKAQVPVGEREGGLIAGFYQQRSHEI
IDMNECLIQQSENDRVVQAIKEICGKYGIKAYNEERHKGWLRHIMVRYGAVTGEMMVVFITRTNDFPHKAKIVEEITAAF
PHVKSIVQNINPKKTNVIFGDETTVIWGEEYIYDTIGGIKFAISARSFYQVNPEQTKVLYDKALEYAELNGEETVIDAYC
GIGTISLFLAKQAKKVYGVEIVPEAIEDAKRNAELNGIENAEFEVGEAEVVIPKWYEEGIAADTLVVDPPRKGCDEALLR
TIIEMKPKRVVYVSCNPGTLARDLRVLEDGGYVTREVQPVDMFPHTSHVECVTKLVLKGAVD
>Mature_462_residues
MKQKAPVQKNEYYNVVFEDLTHEGAGVAKVDGFPIFVPNALPDEKAQIKVTRVKKGFAFGRLIDLKEESRFRTDAPCPIY
KQCGGCQLQHINYEGQLLYKQKQVKDVLERIGKLDLSRITVHPTLGMEDPWNYRNKAQVPVGEREGGLIAGFYQQRSHEI
IDMNECLIQQSENDRVVQAIKEICGKYGIKAYNEERHKGWLRHIMVRYGAVTGEMMVVFITRTNDFPHKAKIVEEITAAF
PHVKSIVQNINPKKTNVIFGDETTVIWGEEYIYDTIGGIKFAISARSFYQVNPEQTKVLYDKALEYAELNGEETVIDAYC
GIGTISLFLAKQAKKVYGVEIVPEAIEDAKRNAELNGIENAEFEVGEAEVVIPKWYEEGIAADTLVVDPPRKGCDEALLR
TIIEMKPKRVVYVSCNPGTLARDLRVLEDGGYVTREVQPVDMFPHTSHVECVTKLVLKGAVD

Specific function: Could Be A 23s rRNA (Uracil-5-)-Methyltransferase. [C]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=345, Percent_Identity=30.4347826086957, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI51173878, Length=345, Percent_Identity=30.4347826086957, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI269784680, Length=203, Percent_Identity=33.0049261083744, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI269784676, Length=203, Percent_Identity=33.0049261083744, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI20149688, Length=203, Percent_Identity=33.0049261083744, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI269784678, Length=203, Percent_Identity=33.0049261083744, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1789148, Length=443, Percent_Identity=31.1512415349887, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1787083, Length=384, Percent_Identity=27.0833333333333, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1790403, Length=210, Percent_Identity=29.5238095238095, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71997438, Length=164, Percent_Identity=33.5365853658537, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6322909, Length=448, Percent_Identity=25.2232142857143, Blast_Score=96, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24666579, Length=411, Percent_Identity=31.3868613138686, Blast_Score=171, Evalue=7e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280 [H]

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr [H]

EC number: NA

Molecular weight: Translated: 52176; Mature: 52176

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS50926 TRAM ; PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKAPVQKNEYYNVVFEDLTHEGAGVAKVDGFPIFVPNALPDEKAQIKVTRVKKGFAFG
CCCCCCCCCCCHHHHEEHHHCCCCCCEEEECCEEEECCCCCCCCCCEEEEEEECCCCCHH
RLIDLKEESRFRTDAPCPIYKQCGGCQLQHINYEGQLLYKQKQVKDVLERIGKLDLSRIT
EEEECCHHCCCCCCCCCCHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHCCCCEEEEE
VHPTLGMEDPWNYRNKAQVPVGEREGGLIAGFYQQRSHEIIDMNECLIQQSENDRVVQAI
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCCEECCHHHHHHCCCCCHHHHHH
KEICGKYGIKAYNEERHKGWLRHIMVRYGAVTGEMMVVFITRTNDFPHKAKIVEEITAAF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
PHVKSIVQNINPKKTNVIFGDETTVIWGEEYIYDTIGGIKFAISARSFYQVNPEQTKVLY
HHHHHHHHCCCCCCCEEEECCCEEEEECCHHHHHHHCCEEEEEECCEEEEECHHHHHHHH
DKALEYAELNGEETVIDAYCGIGTISLFLAKQAKKVYGVEIVPEAIEDAKRNAELNGIEN
HHHHHHHHCCCCCEEEEHHHCHHHHHHHHHHHHHHHHCEEECHHHHHHHHHCCCCCCCCC
AEFEVGEAEVVIPKWYEEGIAADTLVVDPPRKGCDEALLRTIIEMKPKRVVYVSCNPGTL
CCEECCCEEEEEECHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCH
ARDLRVLEDGGYVTREVQPVDMFPHTSHVECVTKLVLKGAVD
HHHHEEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQKAPVQKNEYYNVVFEDLTHEGAGVAKVDGFPIFVPNALPDEKAQIKVTRVKKGFAFG
CCCCCCCCCCCHHHHEEHHHCCCCCCEEEECCEEEECCCCCCCCCCEEEEEEECCCCCHH
RLIDLKEESRFRTDAPCPIYKQCGGCQLQHINYEGQLLYKQKQVKDVLERIGKLDLSRIT
EEEECCHHCCCCCCCCCCHHHHCCCCEEEEECCCCEEEEHHHHHHHHHHHHCCCCEEEEE
VHPTLGMEDPWNYRNKAQVPVGEREGGLIAGFYQQRSHEIIDMNECLIQQSENDRVVQAI
EECCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHCCCCEECCHHHHHHCCCCCHHHHHH
KEICGKYGIKAYNEERHKGWLRHIMVRYGAVTGEMMVVFITRTNDFPHKAKIVEEITAAF
HHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
PHVKSIVQNINPKKTNVIFGDETTVIWGEEYIYDTIGGIKFAISARSFYQVNPEQTKVLY
HHHHHHHHCCCCCCCEEEECCCEEEEECCHHHHHHHCCEEEEEECCEEEEECHHHHHHHH
DKALEYAELNGEETVIDAYCGIGTISLFLAKQAKKVYGVEIVPEAIEDAKRNAELNGIEN
HHHHHHHHCCCCCEEEEHHHCHHHHHHHHHHHHHHHHCEEECHHHHHHHHHCCCCCCCCC
AEFEVGEAEVVIPKWYEEGIAADTLVVDPPRKGCDEALLRTIIEMKPKRVVYVSCNPGTL
CCEECCCEEEEEECHHCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCH
ARDLRVLEDGGYVTREVQPVDMFPHTSHVECVTKLVLKGAVD
HHHHEEEECCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]