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The map label for this gene is purD [H]

Identifier: 52784505

GI number: 52784505

Start: 708634

End: 709902

Strand: Direct

Name: purD [H]

Synonym: BLi00704

Alternate gene names: 52784505

Gene position: 708634-709902 (Clockwise)

Preceding gene: 52784504

Following gene: 52784507

Centisome position: 16.78

GC content: 53.03

Gene sequence:

>1269_bases
GTGAACGTATTGATTATCGGCAGAGGCGGCAGGGAGCACACGCTCGCCTGGAAAGCGGCGCAAAGCCCTCTTGTCGGCAC
TGTATATGCGGCGCCCGGCAATGACGGAATGGCCGGGACCGCCGTACTCGTTGATCTTGACGAAGGCAATCACGAAGGGC
TTCTCGCTTTCGCAAAGGAAAACAATATCGGACTGACGATCGTCGGTCCTGAGGTCCCTTTAATCGAAGGGCTTGTTGAT
GCATTTGAAGAAGCGGGCCTTCCGGTGTTTGGACCGTCGAAAAATGCAGCTGTGATTGAAGGAAGCAAGCAGTTTGCCAA
GGATCTTATGAAAAAATACCAGATTCCGACAGCAGAATACGAAACGTTTACATCTTTTGAAAACGCAAAGGCGTATGTTG
AGGAAAAAGGCGCTCCAATCGTTATTAAAGCCGACGGGCTTGCCGCCGGAAAAGGCGTCACAGTCGCGATGACAGCCGAA
GAAGCGGTTCAGTGTCTGCATGACTTTTTAGAAGATGAAAAGTTCGGCGAAGCAAGCGCATCTGTCGTCATCGAGGAATT
TTTGAGCGGTGAAGAATTTTCGCTGATGGCTTTTGTCAAAGGTGAGAAAGTGTATCCGATGGTGATTGCACAGGATCACA
AGCGGGCATTTGACGGAGACAAGGGCCCGAATACAGGGGGAATGGGCGCGTATTCTCCGGTGCCGCAGATTTCGGATGCG
GTCGTCGAAGACGCCGTTAAAACGATTTTGGAGCCGGCGGCCGAAGCGATGGTTAAAGAAGGCCGTTCGTTTACGGGAGT
CTTGTATGCCGGGTTAATGCTGACCGAAAAAGGAACAAAAGTGATCGAATTCAACGCGAGATTCGGGGATCCCGAAACCC
AGGTCGTCCTGCCGCGTCTTGAATCAGACCTTGTCCAGGTCTTGGTTGATCTCCTCGCAGACAGAGAAGTGGAGCTGAAG
TGGAAGGATACGGCGGCAGTCACCGTTGTTCTTGCATCTGACGGCTATCCGGAAAGCTATGAAAAAGGCGCGCCGATCGG
CACGCTTGAGCCTGACATGGAGCAGACCGTCGTCTTTCACGCAGGCACGAAAAAAAGCGGCGATCAGTTTGTGACGAACG
GCGGCCGCGTCGCCAATGTCACCGCGTTTGCAGAGACGTTTGATGCGGCAAGGGAAGCAGCCTATCAAGCGGTGGATCGA
GTAAGAAAACCGGGGCTGTTTTACCGGATGGACATCGGCTCGCGTGCGCTCAAGGCAGCACAGAAATAA

Upstream 100 bases:

>100_bases
TCGGTTCGTGATGAAGATTCAATTAAAAAAGCGGATGAATACGGGATCGCGATGGTGTTCACCGGAATCCGCCATTTCAA
ACATTAAGGGAGTGACCAGC

Downstream 100 bases:

>100_bases
TAGGTGAAGAGACAGAGCGGGAGCACCCGTTTTGTCTCTTTTTTTGAGCTCAATTTTTTTGGATGATCTTGATCATCGAT
TCAGCCATCAATTCGGGCGG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MNVLIIGRGGREHTLAWKAAQSPLVGTVYAAPGNDGMAGTAVLVDLDEGNHEGLLAFAKENNIGLTIVGPEVPLIEGLVD
AFEEAGLPVFGPSKNAAVIEGSKQFAKDLMKKYQIPTAEYETFTSFENAKAYVEEKGAPIVIKADGLAAGKGVTVAMTAE
EAVQCLHDFLEDEKFGEASASVVIEEFLSGEEFSLMAFVKGEKVYPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISDA
VVEDAVKTILEPAAEAMVKEGRSFTGVLYAGLMLTEKGTKVIEFNARFGDPETQVVLPRLESDLVQVLVDLLADREVELK
WKDTAAVTVVLASDGYPESYEKGAPIGTLEPDMEQTVVFHAGTKKSGDQFVTNGGRVANVTAFAETFDAAREAAYQAVDR
VRKPGLFYRMDIGSRALKAAQK

Sequences:

>Translated_422_residues
MNVLIIGRGGREHTLAWKAAQSPLVGTVYAAPGNDGMAGTAVLVDLDEGNHEGLLAFAKENNIGLTIVGPEVPLIEGLVD
AFEEAGLPVFGPSKNAAVIEGSKQFAKDLMKKYQIPTAEYETFTSFENAKAYVEEKGAPIVIKADGLAAGKGVTVAMTAE
EAVQCLHDFLEDEKFGEASASVVIEEFLSGEEFSLMAFVKGEKVYPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISDA
VVEDAVKTILEPAAEAMVKEGRSFTGVLYAGLMLTEKGTKVIEFNARFGDPETQVVLPRLESDLVQVLVDLLADREVELK
WKDTAAVTVVLASDGYPESYEKGAPIGTLEPDMEQTVVFHAGTKKSGDQFVTNGGRVANVTAFAETFDAAREAAYQAVDR
VRKPGLFYRMDIGSRALKAAQK
>Mature_422_residues
MNVLIIGRGGREHTLAWKAAQSPLVGTVYAAPGNDGMAGTAVLVDLDEGNHEGLLAFAKENNIGLTIVGPEVPLIEGLVD
AFEEAGLPVFGPSKNAAVIEGSKQFAKDLMKKYQIPTAEYETFTSFENAKAYVEEKGAPIVIKADGLAAGKGVTVAMTAE
EAVQCLHDFLEDEKFGEASASVVIEEFLSGEEFSLMAFVKGEKVYPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISDA
VVEDAVKTILEPAAEAMVKEGRSFTGVLYAGLMLTEKGTKVIEFNARFGDPETQVVLPRLESDLVQVLVDLLADREVELK
WKDTAAVTVVLASDGYPESYEKGAPIGTLEPDMEQTVVFHAGTKKSGDQFVTNGGRVANVTAFAETFDAAREAAYQAVDR
VRKPGLFYRMDIGSRALKAAQK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=428, Percent_Identity=46.0280373831776, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI4503915, Length=428, Percent_Identity=46.0280373831776, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI209869995, Length=428, Percent_Identity=46.0280373831776, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI209869993, Length=428, Percent_Identity=46.0280373831776, Blast_Score=366, Evalue=1e-101,
Organism=Escherichia coli, GI1790438, Length=431, Percent_Identity=51.9721577726218, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17567511, Length=423, Percent_Identity=41.6075650118203, Blast_Score=298, Evalue=3e-81,
Organism=Saccharomyces cerevisiae, GI6321203, Length=445, Percent_Identity=43.3707865168539, Blast_Score=337, Evalue=3e-93,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=46.2790697674419, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=46.0648148148148, Blast_Score=343, Evalue=1e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45136; Mature: 45136

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLIIGRGGREHTLAWKAAQSPLVGTVYAAPGNDGMAGTAVLVDLDEGNHEGLLAFAKE
CEEEEEECCCCCCEEEECCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEEEEC
NNIGLTIVGPEVPLIEGLVDAFEEAGLPVFGPSKNAAVIEGSKQFAKDLMKKYQIPTAEY
CCEEEEEECCCCHHHHHHHHHHHHCCCCEECCCCCCEEEECCHHHHHHHHHHHCCCCCCH
ETFTSFENAKAYVEEKGAPIVIKADGLAAGKGVTVAMTAEEAVQCLHDFLEDEKFGEASA
HHHHCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHH
SVVIEEFLSGEEFSLMAFVKGEKVYPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISDA
HHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
VVEDAVKTILEPAAEAMVKEGRSFTGVLYAGLMLTEKGTKVIEFNARFGDPETQVVLPRL
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHEEEEEECCCCEEEEEECCCCCCCCEEEECHH
ESDLVQVLVDLLADREVELKWKDTAAVTVVLASDGYPESYEKGAPIGTLEPDMEQTVVFH
HHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCCCCCHHHCCCCCCCCCCCCCCEEEEE
AGTKKSGDQFVTNGGRVANVTAFAETFDAAREAAYQAVDRVRKPGLFYRMDIGSRALKAA
CCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
QK
CC
>Mature Secondary Structure
MNVLIIGRGGREHTLAWKAAQSPLVGTVYAAPGNDGMAGTAVLVDLDEGNHEGLLAFAKE
CEEEEEECCCCCCEEEECCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEEEEEEC
NNIGLTIVGPEVPLIEGLVDAFEEAGLPVFGPSKNAAVIEGSKQFAKDLMKKYQIPTAEY
CCEEEEEECCCCHHHHHHHHHHHHCCCCEECCCCCCEEEECCHHHHHHHHHHHCCCCCCH
ETFTSFENAKAYVEEKGAPIVIKADGLAAGKGVTVAMTAEEAVQCLHDFLEDEKFGEASA
HHHHCHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCCHHH
SVVIEEFLSGEEFSLMAFVKGEKVYPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISDA
HHHHHHHHCCCCEEEEEEECCCEEEEEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
VVEDAVKTILEPAAEAMVKEGRSFTGVLYAGLMLTEKGTKVIEFNARFGDPETQVVLPRL
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHEEEEEECCCCEEEEEECCCCCCCCEEEECHH
ESDLVQVLVDLLADREVELKWKDTAAVTVVLASDGYPESYEKGAPIGTLEPDMEQTVVFH
HHHHHHHHHHHHCCCCEEEEEECCEEEEEEEECCCCCCHHHCCCCCCCCCCCCCCEEEEE
AGTKKSGDQFVTNGGRVANVTAFAETFDAAREAAYQAVDRVRKPGLFYRMDIGSRALKAA
CCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHH
QK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 8969499; 9384377 [H]