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The map label for this gene is purC [H]

Identifier: 52784497

GI number: 52784497

Start: 700067

End: 700783

Strand: Direct

Name: purC [H]

Synonym: BLi00696

Alternate gene names: 52784497

Gene position: 700067-700783 (Clockwise)

Preceding gene: 52784496

Following gene: 52784498

Centisome position: 16.58

GC content: 45.19

Gene sequence:

>717_bases
GTGAATATTGTGAAAAATGAACTTTTATACGAAGGAAAAGCGAAAAAGATTTACCGGACGGATGATGACAACACTTTGTT
TGTCGAATACAAAAACTCAGCGACGGCTTTTAACGGAGAAAAAAAAGCCGAGATTGACGGAAAAGGCCGTTTAAATAACG
AAATTTCCAGCCTGATTTTCACAAGGCTTCACGAGAAGGGAATCAACAACCACTTTGTTGAACGAATTTCTGAAACGGAG
CAGCTCATTAAAAAAGTGACGATCATCCCGCTTGAAGTCGTTGTCCGCAATGTCGTTGCCGGAAGCATGTCCAAAAGGCT
CGGCATTCCGGAAGGCACGGAGCTGGGGCAGCCGATTATCGAGTTTTACTACAAGGATGACGCGCTCGGTGATCCGTTGA
TCACAGAAGACCATATCCGGATTTTAAAAGCAGCTGAGCCTGAACAGGTGGAGAAAATCAAATCGATTACGAGACAGGTA
AACGAAGAGCTGCAGACGATTTTCGCGGCTTGCGATGTCAGATTGATAGATTTCAAGCTTGAGTTCGGGCTCGATAAAGA
CGGCGAGGTCCTGCTGGCCGATGAGATTTCCCCGGATACGTGCCGGCTGTGGGATCAAGAAACGAATGAAAAGCTTGATA
AAGATGTGTTCAGAAGAAATCTGGGCGGATTGACGGATGCATACGAAGAAATATTCAAAAGACTTGGAGGCATGTAA

Upstream 100 bases:

>100_bases
TTTTTGAACGTTTAGGCTTGGCATAGAATAGACCGCCCGCTTTTACAGGCGGGGGTCTAAGTGCTTAAATATTTCCAACA
TTCGGGTTAGGAGGCCTTCT

Downstream 100 bases:

>100_bases
TATGTACAAAGTGAAAGTGTATGTCAGCTTAAAAGAGAGTGTTTTAGATCCTCAGGGGAGCGCCGTTCAGCATGCTTTGC
ACAGCATGTCCTACAAAGAA

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase; Vegetative protein 286A; VEG286A [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MNIVKNELLYEGKAKKIYRTDDDNTLFVEYKNSATAFNGEKKAEIDGKGRLNNEISSLIFTRLHEKGINNHFVERISETE
QLIKKVTIIPLEVVVRNVVAGSMSKRLGIPEGTELGQPIIEFYYKDDALGDPLITEDHIRILKAAEPEQVEKIKSITRQV
NEELQTIFAACDVRLIDFKLEFGLDKDGEVLLADEISPDTCRLWDQETNEKLDKDVFRRNLGGLTDAYEEIFKRLGGM

Sequences:

>Translated_238_residues
MNIVKNELLYEGKAKKIYRTDDDNTLFVEYKNSATAFNGEKKAEIDGKGRLNNEISSLIFTRLHEKGINNHFVERISETE
QLIKKVTIIPLEVVVRNVVAGSMSKRLGIPEGTELGQPIIEFYYKDDALGDPLITEDHIRILKAAEPEQVEKIKSITRQV
NEELQTIFAACDVRLIDFKLEFGLDKDGEVLLADEISPDTCRLWDQETNEKLDKDVFRRNLGGLTDAYEEIFKRLGGM
>Mature_238_residues
MNIVKNELLYEGKAKKIYRTDDDNTLFVEYKNSATAFNGEKKAEIDGKGRLNNEISSLIFTRLHEKGINNHFVERISETE
QLIKKVTIIPLEVVVRNVVAGSMSKRLGIPEGTELGQPIIEFYYKDDALGDPLITEDHIRILKAAEPEQVEKIKSITRQV
NEELQTIFAACDVRLIDFKLEFGLDKDGEVLLADEISPDTCRLWDQETNEKLDKDVFRRNLGGLTDAYEEIFKRLGGM

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=228, Percent_Identity=32.8947368421053, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI5453539, Length=228, Percent_Identity=32.8947368421053, Blast_Score=118, Evalue=5e-27,
Organism=Homo sapiens, GI119220559, Length=235, Percent_Identity=31.4893617021277, Blast_Score=108, Evalue=6e-24,
Organism=Escherichia coli, GI1788820, Length=228, Percent_Identity=44.7368421052632, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17531275, Length=225, Percent_Identity=37.3333333333333, Blast_Score=134, Evalue=5e-32,
Organism=Drosophila melanogaster, GI18860083, Length=237, Percent_Identity=32.0675105485232, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24583917, Length=239, Percent_Identity=32.2175732217573, Blast_Score=109, Evalue=1e-24,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 27151; Mature: 27151

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01057 SAICAR_SYNTHETASE_1 ; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIVKNELLYEGKAKKIYRTDDDNTLFVEYKNSATAFNGEKKAEIDGKGRLNNEISSLIF
CCCHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
TRLHEKGINNHFVERISETEQLIKKVTIIPLEVVVRNVVAGSMSKRLGIPEGTELGQPII
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCHHHH
EFYYKDDALGDPLITEDHIRILKAAEPEQVEKIKSITRQVNEELQTIFAACDVRLIDFKL
HHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEE
EFGLDKDGEVLLADEISPDTCRLWDQETNEKLDKDVFRRNLGGLTDAYEEIFKRLGGM
EECCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIVKNELLYEGKAKKIYRTDDDNTLFVEYKNSATAFNGEKKAEIDGKGRLNNEISSLIF
CCCHHHHHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
TRLHEKGINNHFVERISETEQLIKKVTIIPLEVVVRNVVAGSMSKRLGIPEGTELGQPII
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCHHHH
EFYYKDDALGDPLITEDHIRILKAAEPEQVEKIKSITRQVNEELQTIFAACDVRLIDFKL
HHHHCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEE
EFGLDKDGEVLLADEISPDTCRLWDQETNEKLDKDVFRRNLGGLTDAYEEIFKRLGGM
EECCCCCCCEEEECCCCCCHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 9298659 [H]