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The map label for this gene is potB [H]

Identifier: 52784221

GI number: 52784221

Start: 376274

End: 377140

Strand: Direct

Name: potB [H]

Synonym: BLi00399

Alternate gene names: 52784221

Gene position: 376274-377140 (Clockwise)

Preceding gene: 52784220

Following gene: 52784222

Centisome position: 8.91

GC content: 46.14

Gene sequence:

>867_bases
ATGAGGGCTGTTGAACAAAAACGGTTTTATTGGTTTTTGCTAGTTCCTGTCCTTTTATTTCTCGGTGTTTTTCTTGTTTT
GCCGCTTGTTCTGCTGCTGTTATTAAGTTTCAGAGACGTGGACGCTATGATGAATACGCTCGATACGTATAGTTTTTCTC
AATATGTCGAGGTGTTCTCTTCAGACGTTTATTTGAAAACAATCGGTATAACAATTTGGACGGCGTTGAAGACCACTGTT
TTATGCCTTGTGATGGCGTATCCGGCGGCATATTTACTCGTTAAGGCGCCAACCGGCAGGCTGCGGGCTTTGTTTTACAT
TTTGCTTGTTTCCCCTTTATTGACCAGTGTTGTCATTCGGACGTTTTCCTGGATTGTGCTGCTTTCGCAAAACGGGATCG
TCAACGAGGCGCTTATCAGTATGAAGATGATTGAAAAGCCCCTGCCGCTTTTATGGAATATGAACGCAGTCATCATTGCA
TATGTCCAGGTTATGCTGCCGTTTGCAGTACTGCCGCTTGCCAGTTCTTTAAGCGAAGTGAAACCTGATCTTAAATTTGC
TTCGATGAGCCTTGGAGCGGGCAGGCTGCGGACATTTTTTTTCGTTACCTTGCCGATGACGGTTCCGGGATTGCTGACAG
GAGCAATGATCGTGTTTTCGCTTTCTGCCGGAAGCTATATTACGCCTCTTCTCGTCGGGGGAAGGATGCAGCCGCTCCTG
CCGTTGTCCATCTATCAGCAGGTCATGCAAGTCTTTCATCTTCCTCTTGCAGCCGCAATGTCGATTACTTTATTAGCCGT
TGTCAGCGTGGTTATTGGAATCCTTGGCATTTTCCAAAAGCGTTGGGAGGCGAGAATAAATGGGTAG

Upstream 100 bases:

>100_bases
CCCGATCCTCAATTCCCTAGATTGCCGAAGGCGGGAGATCAAGTGTATCTTTCTTTGGAGCCGGGCGATTTTGTTCCGCT
GGATGTTAAGGAGGATGCAG

Downstream 100 bases:

>100_bases
GCGATTGCGACGTATGCGCAGGGGAAAATGGATTGATGCGTGTGTTTGGACAGCGGGTTTATTGGTATACTTCTTTTTGG
CGCTGCCCGTCTTCGTGATC

Product: hypothetical protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRAVEQKRFYWFLLVPVLLFLGVFLVLPLVLLLLLSFRDVDAMMNTLDTYSFSQYVEVFSSDVYLKTIGITIWTALKTTV
LCLVMAYPAAYLLVKAPTGRLRALFYILLVSPLLTSVVIRTFSWIVLLSQNGIVNEALISMKMIEKPLPLLWNMNAVIIA
YVQVMLPFAVLPLASSLSEVKPDLKFASMSLGAGRLRTFFFVTLPMTVPGLLTGAMIVFSLSAGSYITPLLVGGRMQPLL
PLSIYQQVMQVFHLPLAAAMSITLLAVVSVVIGILGIFQKRWEARING

Sequences:

>Translated_288_residues
MRAVEQKRFYWFLLVPVLLFLGVFLVLPLVLLLLLSFRDVDAMMNTLDTYSFSQYVEVFSSDVYLKTIGITIWTALKTTV
LCLVMAYPAAYLLVKAPTGRLRALFYILLVSPLLTSVVIRTFSWIVLLSQNGIVNEALISMKMIEKPLPLLWNMNAVIIA
YVQVMLPFAVLPLASSLSEVKPDLKFASMSLGAGRLRTFFFVTLPMTVPGLLTGAMIVFSLSAGSYITPLLVGGRMQPLL
PLSIYQQVMQVFHLPLAAAMSITLLAVVSVVIGILGIFQKRWEARING
>Mature_288_residues
MRAVEQKRFYWFLLVPVLLFLGVFLVLPLVLLLLLSFRDVDAMMNTLDTYSFSQYVEVFSSDVYLKTIGITIWTALKTTV
LCLVMAYPAAYLLVKAPTGRLRALFYILLVSPLLTSVVIRTFSWIVLLSQNGIVNEALISMKMIEKPLPLLWNMNAVIIA
YVQVMLPFAVLPLASSLSEVKPDLKFASMSLGAGRLRTFFFVTLPMTVPGLLTGAMIVFSLSAGSYITPLLVGGRMQPLL
PLSIYQQVMQVFHLPLAAAMSITLLAVVSVVIGILGIFQKRWEARING

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=33.0827067669173, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1787080, Length=220, Percent_Identity=30.9090909090909, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1787713, Length=239, Percent_Identity=28.4518828451883, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787081, Length=258, Percent_Identity=24.8062015503876, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32001; Mature: 32001

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]