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The map label for this gene is nucA [H]

Identifier: 52784211

GI number: 52784211

Start: 365335

End: 365781

Strand: Reverse

Name: nucA [H]

Synonym: BLi00389

Alternate gene names: 52784211

Gene position: 365781-365335 (Counterclockwise)

Preceding gene: 52784214

Following gene: 52784210

Centisome position: 8.66

GC content: 50.34

Gene sequence:

>447_bases
ATGGACAAGATTAAATCTTTGCTGATCACCTTGCTCGCCGTCGCCGTCGTGATCATCGGTATTGTATCTGGCGACTTTTT
CACTTCAGAACACCAGCCTTCGGGCCGGCAAAACGGCTCAAACTACGATGAAGTGCTGATTTTTCCTTCGGACCGCTATC
CTGAAACAGGCGCACACATTCGAAAGGCTATCAAAAAAGGGCACTCCGAAATCTGTACGATCGACAGGGACGGAGCCGCC
GAACGCCGAAAGGATTCATTGAAAGATGTTCCTTCCAAATCAGGCTATGACCGCGACGAGTGGCCGATGGCCATGTGCGA
AGAAGGCGGAACAGGTGCTTCTGTCGAGTATATCAGCCCGTCTGATAACCGTGGAGCAGGATCTTGGGTGGGCAATCAGG
TAAGCGATTATCCGGACGGGACGAAAGTACTTTTCAAGATTAATTAA

Upstream 100 bases:

>100_bases
TCCCCTTTATTTCTCAAATCATTTTTACGATAATAAAGTTGTATTTACTTCCGGTCCACTGCGGCCGAATGATTTAACAT
AAGAAAGCAGGACAACAAGA

Downstream 100 bases:

>100_bases
CACTGGGGGTACGGTCTTTGATCAAAAAGTGGGAAGCACAAGAAATGACGATGTCATACCATCAGTTTACCGTTTATCAG
AAAGGCGGAAAGCCGCCCGT

Product: NucA

Products: NA

Alternate protein names: Competence-specific nuclease [H]

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MDKIKSLLITLLAVAVVIIGIVSGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHIRKAIKKGHSEICTIDRDGAA
ERRKDSLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISPSDNRGAGSWVGNQVSDYPDGTKVLFKIN

Sequences:

>Translated_148_residues
MDKIKSLLITLLAVAVVIIGIVSGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHIRKAIKKGHSEICTIDRDGAA
ERRKDSLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISPSDNRGAGSWVGNQVSDYPDGTKVLFKIN
>Mature_148_residues
MDKIKSLLITLLAVAVVIIGIVSGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHIRKAIKKGHSEICTIDRDGAA
ERRKDSLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISPSDNRGAGSWVGNQVSDYPDGTKVLFKIN

Specific function: By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed. Degrades both double- stranded, linear and covalently closed circular DNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nucB [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 16180; Mature: 16180

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKIKSLLITLLAVAVVIIGIVSGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHH
RKAIKKGHSEICTIDRDGAAERRKDSLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISP
HHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCEEEEECC
SDNRGAGSWVGNQVSDYPDGTKVLFKIN
CCCCCCCCCCCCCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MDKIKSLLITLLAVAVVIIGIVSGDFFTSEHQPSGRQNGSNYDEVLIFPSDRYPETGAHI
CHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHH
RKAIKKGHSEICTIDRDGAAERRKDSLKDVPSKSGYDRDEWPMAMCEEGGTGASVEYISP
HHHHHCCCCCEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCEEEEECC
SDNRGAGSWVGNQVSDYPDGTKVLFKIN
CCCCCCCCCCCCCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7704255; 7746143; 8969502; 9384377; 2841296 [H]