Click here to switch to the map view.

The map label for this gene is yckE [H]

Identifier: 52784209

GI number: 52784209

Start: 363444

End: 364880

Strand: Direct

Name: yckE [H]

Synonym: BLi00387

Alternate gene names: 52784209

Gene position: 363444-364880 (Clockwise)

Preceding gene: 52784208

Following gene: 52784212

Centisome position: 8.61

GC content: 45.23

Gene sequence:

>1437_bases
ATGATTCATCAGCGACTACGCCCATTTCCAGATCATTTCCTATGGGGATCAGCATCTGCAGCCTACCAGATCGAAGGAGC
CTGGAATGAAGACGGCAAGGGGCTTTCGGTTTGGGATGTGTTCACAAAGATTCCCGGAAAAACGTTTAAAGGAAGCAATG
GAGACATTGCGGTCGACCATTACCACCGTTTTAAAGAAGATGTTGCTTTAATGGCTGAGATGGGGTTAAAAGCATACCGC
TTTTCAGTCAGCTGGCCGAGGATTTTTCCGCAGGGCAGGGGAGAAGCCAATGAATCGGGTTTGAGATTTTACGATGACTT
GATTAATGAGCTCCTTGCTCATGATATTGAACCTGTTTTGACGCTTTATCATTGGGATCTTCCCCAGGCTTTAATGGATG
AATACGGCGGCTTTGAATCCAGAAGAATTATAGAGGATTTCAATGCGTATTGCGTTACGCTCTATAAGCGGTATGGAGGC
AGGGTTAAATATTGGGTGTCCCTTAACGAGCAAAATTACAACTTTAATCACGGCTTTATCACAGCGATGCATCCCCCGGG
TGTGAAGGATAGAAAACGCTTCTATGAGGCTAACCATATTGCATTTCTAGCGAATGCAAAGGCAATCGATTCTTTTCGCC
GGTATGTTCCCGATGGAAAAATCGGACCGAGTTTTGCTTATTCGCCTGCATATCCCCTCTCCAGCCGTCCGGATGATATC
CTCGCCTTCGAGAATGCCGAGGAATTTACAAACTATTGGTGGCTTGATATGTACTGCCGGGGAACATATCCGGACATCCC
TTTAAAGTATCTGAAGGAAAAAGGCTGGGCGCCGACAATTGAAGATGGGGATATGGAGCTTTTAGCAAAAGGAAAACCCG
ATTTTGTCGGTGTGAATTATTATCAAACCATCACATATGAAATGAATCCTCTTGACGGGGTATCGGAAGGAAAGATGAAT
ACGACCGGCCAAAAGGGAAGCAATCAGGAAACAGGCATGCCGGGTTTATACAAAACAAAAAGGAACCCTCATCTTGAGAC
ATCGAATTGGGATTGGGCCATTGACCCGATCGGTCTAAGAATCGGACTTCGCAGGATTTCCAGCCGCTATGGGCTTCCGC
TTTTCATAACGGAAAACGGGCTGGGGGAATTCGATAAGGTTGAAAATGACGGCACGATTCATGATGACTACAGAATTGCC
TACTTGCGTGCGCACCTTGAACAATGCAGACAGGCTTTAAACGACGGAGTCGATTTAATCGGCTACTGCAGCTGGTCATT
TACAGATTTATTGAGCTGGCTGAACGGCTACCAGAAAAGATACGGTTTCGTTTATATTAACCGTGATGAAGAGAATGTAA
AGGACTTGAAGAGAATTAAAAAAGACAGCTTTTACTGGTACCAAAACGTCATTCAGACAAACGGGGAAGAGCTTTAG

Upstream 100 bases:

>100_bases
TTTGGCGGAGCTGTTTTTAGATGACGTTTGATCTGAAACGCGTTTCATAGGATATGATAAGGAGCATCCGGATCAAAATA
TGAAACAGGAGTTGAGCTAT

Downstream 100 bases:

>100_bases
GAGGGGCGCCGGATGAAACCGGCGCCTTTATCCGTTGTTCAAGCCTGTTTAAAATCGAGAAGATAGGTGATCTGTGCAGT
GCCGCTGTGATCAGGCATTA

Product: YckE

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MIHQRLRPFPDHFLWGSASAAYQIEGAWNEDGKGLSVWDVFTKIPGKTFKGSNGDIAVDHYHRFKEDVALMAEMGLKAYR
FSVSWPRIFPQGRGEANESGLRFYDDLINELLAHDIEPVLTLYHWDLPQALMDEYGGFESRRIIEDFNAYCVTLYKRYGG
RVKYWVSLNEQNYNFNHGFITAMHPPGVKDRKRFYEANHIAFLANAKAIDSFRRYVPDGKIGPSFAYSPAYPLSSRPDDI
LAFENAEEFTNYWWLDMYCRGTYPDIPLKYLKEKGWAPTIEDGDMELLAKGKPDFVGVNYYQTITYEMNPLDGVSEGKMN
TTGQKGSNQETGMPGLYKTKRNPHLETSNWDWAIDPIGLRIGLRRISSRYGLPLFITENGLGEFDKVENDGTIHDDYRIA
YLRAHLEQCRQALNDGVDLIGYCSWSFTDLLSWLNGYQKRYGFVYINRDEENVKDLKRIKKDSFYWYQNVIQTNGEEL

Sequences:

>Translated_478_residues
MIHQRLRPFPDHFLWGSASAAYQIEGAWNEDGKGLSVWDVFTKIPGKTFKGSNGDIAVDHYHRFKEDVALMAEMGLKAYR
FSVSWPRIFPQGRGEANESGLRFYDDLINELLAHDIEPVLTLYHWDLPQALMDEYGGFESRRIIEDFNAYCVTLYKRYGG
RVKYWVSLNEQNYNFNHGFITAMHPPGVKDRKRFYEANHIAFLANAKAIDSFRRYVPDGKIGPSFAYSPAYPLSSRPDDI
LAFENAEEFTNYWWLDMYCRGTYPDIPLKYLKEKGWAPTIEDGDMELLAKGKPDFVGVNYYQTITYEMNPLDGVSEGKMN
TTGQKGSNQETGMPGLYKTKRNPHLETSNWDWAIDPIGLRIGLRRISSRYGLPLFITENGLGEFDKVENDGTIHDDYRIA
YLRAHLEQCRQALNDGVDLIGYCSWSFTDLLSWLNGYQKRYGFVYINRDEENVKDLKRIKKDSFYWYQNVIQTNGEEL
>Mature_478_residues
MIHQRLRPFPDHFLWGSASAAYQIEGAWNEDGKGLSVWDVFTKIPGKTFKGSNGDIAVDHYHRFKEDVALMAEMGLKAYR
FSVSWPRIFPQGRGEANESGLRFYDDLINELLAHDIEPVLTLYHWDLPQALMDEYGGFESRRIIEDFNAYCVTLYKRYGG
RVKYWVSLNEQNYNFNHGFITAMHPPGVKDRKRFYEANHIAFLANAKAIDSFRRYVPDGKIGPSFAYSPAYPLSSRPDDI
LAFENAEEFTNYWWLDMYCRGTYPDIPLKYLKEKGWAPTIEDGDMELLAKGKPDFVGVNYYQTITYEMNPLDGVSEGKMN
TTGQKGSNQETGMPGLYKTKRNPHLETSNWDWAIDPIGLRIGLRRISSRYGLPLFITENGLGEFDKVENDGTIHDDYRIA
YLRAHLEQCRQALNDGVDLIGYCSWSFTDLLSWLNGYQKRYGFVYINRDEENVKDLKRIKKDSFYWYQNVIQTNGEEL

Specific function: Is able to catalyze the hydrolysis of aryl-phospho-beta- D-glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Is not essential for growth on arbutin and salicin as the sole carbon source [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI110681710, Length=485, Percent_Identity=33.4020618556701, Blast_Score=250, Evalue=2e-66,
Organism=Homo sapiens, GI32481206, Length=484, Percent_Identity=32.2314049586777, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI13273313, Length=492, Percent_Identity=31.7073170731707, Blast_Score=211, Evalue=8e-55,
Organism=Homo sapiens, GI28376633, Length=479, Percent_Identity=29.4363256784969, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI24497614, Length=491, Percent_Identity=28.9205702647658, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI190360571, Length=85, Percent_Identity=48.2352941176471, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI2367174, Length=501, Percent_Identity=39.9201596806387, Blast_Score=365, Evalue=1e-102,
Organism=Escherichia coli, GI2367270, Length=483, Percent_Identity=39.1304347826087, Blast_Score=341, Evalue=7e-95,
Organism=Escherichia coli, GI1789070, Length=495, Percent_Identity=37.979797979798, Blast_Score=325, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17552856, Length=480, Percent_Identity=30.8333333333333, Blast_Score=218, Evalue=5e-57,
Organism=Caenorhabditis elegans, GI17539390, Length=482, Percent_Identity=28.4232365145228, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI21356577, Length=497, Percent_Identity=31.1871227364185, Blast_Score=209, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 55426; Mature: 55426

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHQRLRPFPDHFLWGSASAAYQIEGAWNEDGKGLSVWDVFTKIPGKTFKGSNGDIAVDH
CCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCEEHHHHHHHCCCCCCCCCCCCEEEHH
YHRFKEDVALMAEMGLKAYRFSVSWPRIFPQGRGEANESGLRFYDDLINELLAHDIEPVL
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
TLYHWDLPQALMDEYGGFESRRIIEDFNAYCVTLYKRYGGRVKYWVSLNEQNYNFNHGFI
EEHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEE
TAMHPPGVKDRKRFYEANHIAFLANAKAIDSFRRYVPDGKIGPSFAYSPAYPLSSRPDDI
EEECCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
LAFENAEEFTNYWWLDMYCRGTYPDIPLKYLKEKGWAPTIEDGDMELLAKGKPDFVGVNY
EEECCHHHHCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCEEECEE
YQTITYEMNPLDGVSEGKMNTTGQKGSNQETGMPGLYKTKRNPHLETSNWDWAIDPIGLR
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEECCHHHH
IGLRRISSRYGLPLFITENGLGEFDKVENDGTIHDDYRIAYLRAHLEQCRQALNDGVDLI
HHHHHHHHHCCCEEEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
GYCSWSFTDLLSWLNGYQKRYGFVYINRDEENVKDLKRIKKDSFYWYQNVIQTNGEEL
EECCCCHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIHQRLRPFPDHFLWGSASAAYQIEGAWNEDGKGLSVWDVFTKIPGKTFKGSNGDIAVDH
CCCCCCCCCCCHHCCCCCCCEEEEECCCCCCCCCEEHHHHHHHCCCCCCCCCCCCEEEHH
YHRFKEDVALMAEMGLKAYRFSVSWPRIFPQGRGEANESGLRFYDDLINELLAHDIEPVL
HHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHH
TLYHWDLPQALMDEYGGFESRRIIEDFNAYCVTLYKRYGGRVKYWVSLNEQNYNFNHGFI
EEHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCEE
TAMHPPGVKDRKRFYEANHIAFLANAKAIDSFRRYVPDGKIGPSFAYSPAYPLSSRPDDI
EEECCCCCHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCE
LAFENAEEFTNYWWLDMYCRGTYPDIPLKYLKEKGWAPTIEDGDMELLAKGKPDFVGVNY
EEECCHHHHCCEEEEEEEECCCCCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCEEECEE
YQTITYEMNPLDGVSEGKMNTTGQKGSNQETGMPGLYKTKRNPHLETSNWDWAIDPIGLR
EEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCEEECCHHHH
IGLRRISSRYGLPLFITENGLGEFDKVENDGTIHDDYRIAYLRAHLEQCRQALNDGVDLI
HHHHHHHHHCCCEEEEECCCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
GYCSWSFTDLLSWLNGYQKRYGFVYINRDEENVKDLKRIKKDSFYWYQNVIQTNGEEL
EECCCCHHHHHHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704255; 8969502; 9384377; 2841296 [H]