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The map label for this gene is ycbF [H]

Identifier: 52784110

GI number: 52784110

Start: 262140

End: 263507

Strand: Direct

Name: ycbF [H]

Synonym: BLi00287

Alternate gene names: 52784110

Gene position: 262140-263507 (Clockwise)

Preceding gene: 52784109

Following gene: 52784111

Centisome position: 6.21

GC content: 54.39

Gene sequence:

>1368_bases
ATGAATACAAACATCCATCAGCATGTAAAAGAGGAGAAAGCATCGCAAACACCGTCTATTGCCGACATGCGGGTTATACC
CGTGGCAGGGCGGGACAGCATGCTTTTGAATTTGAGCGGAGCTCACGGCCCCTTTTTCACACGGAATATCGTGATTTTAA
AGGATTCATCAGGCGCTCTCGGCGTCGGCGAAGTGCCGGGCGGCGAGCGGATCCGCCAGACGCTGGAGCAGGCGAAGCCG
CTCGTCATCGATCAGCCGCTCGGCGCTGTTCAATCGATTTTGCAGTCCGTCAGGCGCCGGTTTAAAGACAGGGATGCCGG
AGGACGCGGGGTGCAGACCTTTGACCAGCGGACGACGATACACGCTGTGACCGCATTGGAAGCAGCTTTGCTCGATTTGC
TCGGAAAATTTCTCGGCGTCCCTGTGGCGGCGCTCTTGGGGGAAGGCCAGCAGCGGGATAAAGTGAAGATGCTCGGCTAT
TTGTTTTACATCGGGGACCGAAAGCGCACGACGCTTCCCTATATCAGTGAACAAGAGGCAAATGACGACTGGTTCCATCT
CCGCCGTGAAGAAGCGTTGACTCCCGAGGCGATCGTCCGTCTTGCGGAGGCAGCCCGAGAACGATACGGATTTCACGATT
TTAAATTAAAAGGCGGCGTCCTTTCCGGAGAAGAAGAAATCGAGGCGGCATCCGCTTTAGCGAAGCGGTTTCCTAAGGCG
AGAATCACCCTTGACCCGAATGGGGCGTGGTCATTGGAGGAAGCGATTGCGCTTTGTAAGGGAAAGCGGGATGTACTTGC
CTACGCGGAAGACCCTTGCGGAGCGGAAGCCGGATACTCAGGCCGGGAAATCATGGCCGAATTCCGCCGTGCGACAGGCA
TTCCGACAGCGACGAATATGATTGCGACAGACTGGCGGCAAATGGGGCATGCGATACAGCTGCACGCCGTTGATATACCG
CTGGCAGATCCGCATTTTTGGACGATGCAGGGATCCGTCCGCGTTGCTCAAATGTGCCATGAATGGGGGCTGATATGGGG
CTCTCACTCCAATAACCATTTTGACGTCTCACTGGCCATGTTCACCCACGTTGCCGCAGCAGCTCCGGGCGATATCACGG
CGATTGATACGCATTGGATATGGCAGGATGGACAGCGTCTCACAAAACAGCCTTTTTCAATTGCTGACGGATATGTCCAA
GTTCCTGATCAGCCTGGGCTCGGCATTGAAATCGACATGGAACGGGTGGAAAAAGCAAATGAACTATATGAGAAAATGAC
AGCAGGCGAGCGCAATGATGCCGCTGCCATGCAGTTTTTGATTGAAAATTGGCGTTTCGACCCTAAACGTCCTTGTCTTG
TTCGATAA

Upstream 100 bases:

>100_bases
CCGATCAAGCGCATCGAACTGCAGAAAGAGAAAGACCCTGCGGACACCACTTTATCCATGTGAAATGTAAGCGCTTTAAT
AAGAGGGAGGCTAAATCGAT

Downstream 100 bases:

>100_bases
TTGCGGAAACAGCGGTTTGAGAGGCCCCTAGCCGACATTGTCATACGCGATATTTTGTCTTACAATATGAACAACTGAAA
GGGGGCCCACAGTCGTGGAA

Product: YcbF

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MNTNIHQHVKEEKASQTPSIADMRVIPVAGRDSMLLNLSGAHGPFFTRNIVILKDSSGALGVGEVPGGERIRQTLEQAKP
LVIDQPLGAVQSILQSVRRRFKDRDAGGRGVQTFDQRTTIHAVTALEAALLDLLGKFLGVPVAALLGEGQQRDKVKMLGY
LFYIGDRKRTTLPYISEQEANDDWFHLRREEALTPEAIVRLAEAARERYGFHDFKLKGGVLSGEEEIEAASALAKRFPKA
RITLDPNGAWSLEEAIALCKGKRDVLAYAEDPCGAEAGYSGREIMAEFRRATGIPTATNMIATDWRQMGHAIQLHAVDIP
LADPHFWTMQGSVRVAQMCHEWGLIWGSHSNNHFDVSLAMFTHVAAAAPGDITAIDTHWIWQDGQRLTKQPFSIADGYVQ
VPDQPGLGIEIDMERVEKANELYEKMTAGERNDAAAMQFLIENWRFDPKRPCLVR

Sequences:

>Translated_455_residues
MNTNIHQHVKEEKASQTPSIADMRVIPVAGRDSMLLNLSGAHGPFFTRNIVILKDSSGALGVGEVPGGERIRQTLEQAKP
LVIDQPLGAVQSILQSVRRRFKDRDAGGRGVQTFDQRTTIHAVTALEAALLDLLGKFLGVPVAALLGEGQQRDKVKMLGY
LFYIGDRKRTTLPYISEQEANDDWFHLRREEALTPEAIVRLAEAARERYGFHDFKLKGGVLSGEEEIEAASALAKRFPKA
RITLDPNGAWSLEEAIALCKGKRDVLAYAEDPCGAEAGYSGREIMAEFRRATGIPTATNMIATDWRQMGHAIQLHAVDIP
LADPHFWTMQGSVRVAQMCHEWGLIWGSHSNNHFDVSLAMFTHVAAAAPGDITAIDTHWIWQDGQRLTKQPFSIADGYVQ
VPDQPGLGIEIDMERVEKANELYEKMTAGERNDAAAMQFLIENWRFDPKRPCLVR
>Mature_455_residues
MNTNIHQHVKEEKASQTPSIADMRVIPVAGRDSMLLNLSGAHGPFFTRNIVILKDSSGALGVGEVPGGERIRQTLEQAKP
LVIDQPLGAVQSILQSVRRRFKDRDAGGRGVQTFDQRTTIHAVTALEAALLDLLGKFLGVPVAALLGEGQQRDKVKMLGY
LFYIGDRKRTTLPYISEQEANDDWFHLRREEALTPEAIVRLAEAARERYGFHDFKLKGGVLSGEEEIEAASALAKRFPKA
RITLDPNGAWSLEEAIALCKGKRDVLAYAEDPCGAEAGYSGREIMAEFRRATGIPTATNMIATDWRQMGHAIQLHAVDIP
LADPHFWTMQGSVRVAQMCHEWGLIWGSHSNNHFDVSLAMFTHVAAAAPGDITAIDTHWIWQDGQRLTKQPFSIADGYVQ
VPDQPGLGIEIDMERVEKANELYEKMTAGERNDAAAMQFLIENWRFDPKRPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=440, Percent_Identity=72.0454545454545, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=442, Percent_Identity=62.6696832579185, Blast_Score=567, Evalue=1e-163,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 50526; Mature: 50526

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTNIHQHVKEEKASQTPSIADMRVIPVAGRDSMLLNLSGAHGPFFTRNIVILKDSSGAL
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCCC
GVGEVPGGERIRQTLEQAKPLVIDQPLGAVQSILQSVRRRFKDRDAGGRGVQTFDQRTTI
CCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
HAVTALEAALLDLLGKFLGVPVAALLGEGQQRDKVKMLGYLFYIGDRKRTTLPYISEQEA
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHEEEEEEECCCCCCCCCCCCCCCC
NDDWFHLRREEALTPEAIVRLAEAARERYGFHDFKLKGGVLSGEEEIEAASALAKRFPKA
CCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
RITLDPNGAWSLEEAIALCKGKRDVLAYAEDPCGAEAGYSGREIMAEFRRATGIPTATNM
EEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
IATDWRQMGHAIQLHAVDIPLADPHFWTMQGSVRVAQMCHEWGLIWGSHSNNHFDVSLAM
HHHHHHHHCCEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCEECCCCCCEEEEEHHH
FTHVAAAAPGDITAIDTHWIWQDGQRLTKQPFSIADGYVQVPDQPGLGIEIDMERVEKAN
HHHHHHCCCCCEEEEECEEEECCCHHHHCCCCCCCCCEEECCCCCCCCEEECHHHHHHHH
ELYEKMTAGERNDAAAMQFLIENWRFDPKRPCLVR
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MNTNIHQHVKEEKASQTPSIADMRVIPVAGRDSMLLNLSGAHGPFFTRNIVILKDSSGAL
CCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEECCCCCCEEEEEEEEEECCCCCC
GVGEVPGGERIRQTLEQAKPLVIDQPLGAVQSILQSVRRRFKDRDAGGRGVQTFDQRTTI
CCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
HAVTALEAALLDLLGKFLGVPVAALLGEGQQRDKVKMLGYLFYIGDRKRTTLPYISEQEA
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHEEEEEEECCCCCCCCCCCCCCCC
NDDWFHLRREEALTPEAIVRLAEAARERYGFHDFKLKGGVLSGEEEIEAASALAKRFPKA
CCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCC
RITLDPNGAWSLEEAIALCKGKRDVLAYAEDPCGAEAGYSGREIMAEFRRATGIPTATNM
EEEECCCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
IATDWRQMGHAIQLHAVDIPLADPHFWTMQGSVRVAQMCHEWGLIWGSHSNNHFDVSLAM
HHHHHHHHCCEEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCEECCCCCCEEEEEHHH
FTHVAAAAPGDITAIDTHWIWQDGQRLTKQPFSIADGYVQVPDQPGLGIEIDMERVEKAN
HHHHHHCCCCCEEEEECEEEECCCHHHHCCCCCCCCCEEECCCCCCCCEEECHHHHHHHH
ELYEKMTAGERNDAAAMQFLIENWRFDPKRPCLVR
HHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]