Click here to switch to the map view.

The map label for this gene is thrZ [H]

Identifier: 52784060

GI number: 52784060

Start: 210927

End: 212846

Strand: Direct

Name: thrZ [H]

Synonym: BLi00234

Alternate gene names: 52784060

Gene position: 210927-212846 (Clockwise)

Preceding gene: 52784056

Following gene: 52784061

Centisome position: 5.0

GC content: 34.53

Gene sequence:

>1920_bases
ATGAGTGAAAGTTTAATAAAAGTTCAATTCAAAGAAGGAAATACGAAGGAATTTCCGAAGGGGATAACAATAAAACGTAT
TGCAGAATCCATTAGTACAACTTTAAGAAAGCACGCTGTTGTAGCAAAGGTTGATGAAAAATTCGTGGATTTAAGCTATA
CCCTTGACAAGGATGTTAAACTCGATATTTTTACTCTAGATTCGAAAGAAGGTTTGTCGGTGTTAAGACATACATCCACC
CATATATTGGCACACGCTGTGAAAAGGCTTTACAGTAGTGTAAGTTTGGGTATTGGACCAGTAATTGAAGATGAGTTTTT
CTATGATTTGAAACTTGAACACAGTTTGTCTCCCGAGGATTTACTAGCAATTGAACGTGAGATGGAAAAGATTATTAAGG
AGAATATAGAAATTAAACGAATTGTTGTCTCTTATGAGGAAGCTGAAAAGTTATTTGAAGAAAATAATGAACCATTAAAG
CTTGAAATATTAAGAGGAATTCCAAAAGGCCAAGAAATAACTCTTTACCAACAGGGAGAATTTATTGATCTTTGTCGCGG
TCCACACCTTCCATCAACTGGTTTCATTAAAGCATTTAAATTAACTCGTGTATCTGGAGCTTATTGGAGAGGAAATCGCC
AAAAAGAAGTTCTTCAACGAGTTTATGGAGTAGCTTTTAAAAAGAAAGACGATTTAAATGAATATCTATATTTTTTAGAA
CAAGCAGCAAAACATGATCATCGACAGTTAGGAAAACAATTAGATTTATTTATGTTCTCAGAGGAAGCACCGGGAATGCC
CTTTTACCTACCTAAAGGACAAATTGTTAGAAAAGAATTGGAGAAATTATCCCGTGAACTTCAAAATAATGCATCGTACG
ATGAGGTCCATACACCGTTTATGATGAATTATCGATTATGGGAGAAATCCGGTCATTGGGATCATTACCAAGAAAATATG
TACTTTTCTGAGGTCGATAATACAAAGTTTGCAATCAAACCAATGAATTGTCCTGGACATATGCTAATCTTTAAAAATAA
TCTTTACTCCTATCGAGATCTACCTATTCGATTGGCGGAATATGGACAAGTTCATCGACATGAATTTAGTGGTTCATTAA
ACGGATTGCTTCGGGTACGCACATTTTGTCAGGATGACGCTCACATCTTTGTTCGAGAAGATCAAATTGAAAGTGAAATT
AAGAAGGTATTTCATTTAATTGACCAAGTGTATCGTACTATTGGTTTTGAGTATTCAGTGGAACTGTCTACTCGGCCAGA
TGATTCATTAGGAAATAATCACCTATGGGAGGTTTCTGAGGGATCATTAAAAAATGTGCTTGACGATTTAGCAATCAAAT
ATCAAGTTAATGAAGGTGATGGGGCTTTTTACGGACCAAAAATTGATTTCCATATTAAAGATGCTTTAAAACGGAGTCAT
CAGTGCGGAACAATCCAACTTGATTTTCAGATGCCTGAAAAATTTGATTTGACTTATATTAATCATAATAACGAAAAAGT
TCGTCCTGTTGTTATCCACCGTGCTATTTTTGGTTCTATTGATCGTTTTTTTGGAATTTTAATAGAGCATTTTGCGGGTG
TTTTTCCTGTTTGGCTTGCCCCTATTCAAGTTCAAATTATTCCTATTTCACATATTCACCTGGAGTATTGTTTAGGGATT
AAGTCGGAACTCAAACAACACGGGATAAGAGTACAAATTAACGAGAGTAATCAAAAGTTAGGGTACAAAATTAGGGAAGC
ACAGGTTCAAAAAATCCCCTATACATTAGTAGTAGGAGACAATGAAATTAAAGAAAACGGAGTAAATGTAAGAAAGTATG
GAGAGGAACAACAAAAAAATGTTTCAATTGAAAGCTTTAAAAAAAATATTTTGCAGCAAATAAAGACACGTAGTGTCTAG

Upstream 100 bases:

>100_bases
TTTGGGGATTAGTGCATTTTTTTATTTTCCACTCAACGTGGCTATTGCAAAGCTAGGGTTTAGAGTGTAAATCAATTTTA
TTAAAAGGGGATGCACTATA

Downstream 100 bases:

>100_bases
TTGAAAGAAAGTATAAAAGTTTTTAATAAATCTTCATCTAATTTGAAGCTATCTTGATAAAAAAGTATTTTAAACAAGTA
AATATCACAAAAAAATGAAT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 639; Mature: 638

Protein sequence:

>639_residues
MSESLIKVQFKEGNTKEFPKGITIKRIAESISTTLRKHAVVAKVDEKFVDLSYTLDKDVKLDIFTLDSKEGLSVLRHTST
HILAHAVKRLYSSVSLGIGPVIEDEFFYDLKLEHSLSPEDLLAIEREMEKIIKENIEIKRIVVSYEEAEKLFEENNEPLK
LEILRGIPKGQEITLYQQGEFIDLCRGPHLPSTGFIKAFKLTRVSGAYWRGNRQKEVLQRVYGVAFKKKDDLNEYLYFLE
QAAKHDHRQLGKQLDLFMFSEEAPGMPFYLPKGQIVRKELEKLSRELQNNASYDEVHTPFMMNYRLWEKSGHWDHYQENM
YFSEVDNTKFAIKPMNCPGHMLIFKNNLYSYRDLPIRLAEYGQVHRHEFSGSLNGLLRVRTFCQDDAHIFVREDQIESEI
KKVFHLIDQVYRTIGFEYSVELSTRPDDSLGNNHLWEVSEGSLKNVLDDLAIKYQVNEGDGAFYGPKIDFHIKDALKRSH
QCGTIQLDFQMPEKFDLTYINHNNEKVRPVVIHRAIFGSIDRFFGILIEHFAGVFPVWLAPIQVQIIPISHIHLEYCLGI
KSELKQHGIRVQINESNQKLGYKIREAQVQKIPYTLVVGDNEIKENGVNVRKYGEEQQKNVSIESFKKNILQQIKTRSV

Sequences:

>Translated_639_residues
MSESLIKVQFKEGNTKEFPKGITIKRIAESISTTLRKHAVVAKVDEKFVDLSYTLDKDVKLDIFTLDSKEGLSVLRHTST
HILAHAVKRLYSSVSLGIGPVIEDEFFYDLKLEHSLSPEDLLAIEREMEKIIKENIEIKRIVVSYEEAEKLFEENNEPLK
LEILRGIPKGQEITLYQQGEFIDLCRGPHLPSTGFIKAFKLTRVSGAYWRGNRQKEVLQRVYGVAFKKKDDLNEYLYFLE
QAAKHDHRQLGKQLDLFMFSEEAPGMPFYLPKGQIVRKELEKLSRELQNNASYDEVHTPFMMNYRLWEKSGHWDHYQENM
YFSEVDNTKFAIKPMNCPGHMLIFKNNLYSYRDLPIRLAEYGQVHRHEFSGSLNGLLRVRTFCQDDAHIFVREDQIESEI
KKVFHLIDQVYRTIGFEYSVELSTRPDDSLGNNHLWEVSEGSLKNVLDDLAIKYQVNEGDGAFYGPKIDFHIKDALKRSH
QCGTIQLDFQMPEKFDLTYINHNNEKVRPVVIHRAIFGSIDRFFGILIEHFAGVFPVWLAPIQVQIIPISHIHLEYCLGI
KSELKQHGIRVQINESNQKLGYKIREAQVQKIPYTLVVGDNEIKENGVNVRKYGEEQQKNVSIESFKKNILQQIKTRSV
>Mature_638_residues
SESLIKVQFKEGNTKEFPKGITIKRIAESISTTLRKHAVVAKVDEKFVDLSYTLDKDVKLDIFTLDSKEGLSVLRHTSTH
ILAHAVKRLYSSVSLGIGPVIEDEFFYDLKLEHSLSPEDLLAIEREMEKIIKENIEIKRIVVSYEEAEKLFEENNEPLKL
EILRGIPKGQEITLYQQGEFIDLCRGPHLPSTGFIKAFKLTRVSGAYWRGNRQKEVLQRVYGVAFKKKDDLNEYLYFLEQ
AAKHDHRQLGKQLDLFMFSEEAPGMPFYLPKGQIVRKELEKLSRELQNNASYDEVHTPFMMNYRLWEKSGHWDHYQENMY
FSEVDNTKFAIKPMNCPGHMLIFKNNLYSYRDLPIRLAEYGQVHRHEFSGSLNGLLRVRTFCQDDAHIFVREDQIESEIK
KVFHLIDQVYRTIGFEYSVELSTRPDDSLGNNHLWEVSEGSLKNVLDDLAIKYQVNEGDGAFYGPKIDFHIKDALKRSHQ
CGTIQLDFQMPEKFDLTYINHNNEKVRPVVIHRAIFGSIDRFFGILIEHFAGVFPVWLAPIQVQIIPISHIHLEYCLGIK
SELKQHGIRVQINESNQKLGYKIREAQVQKIPYTLVVGDNEIKENGVNVRKYGEEQQKNVSIESFKKNILQQIKTRSV

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38570111, Length=642, Percent_Identity=41.1214953271028, Blast_Score=507, Evalue=1e-143,
Organism=Homo sapiens, GI38202255, Length=592, Percent_Identity=42.5675675675676, Blast_Score=494, Evalue=1e-139,
Organism=Homo sapiens, GI20070344, Length=610, Percent_Identity=39.672131147541, Blast_Score=450, Evalue=1e-126,
Organism=Escherichia coli, GI1788013, Length=634, Percent_Identity=42.4290220820189, Blast_Score=574, Evalue=1e-165,
Organism=Escherichia coli, GI1786392, Length=190, Percent_Identity=25.7894736842105, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71984192, Length=589, Percent_Identity=41.9354838709677, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71984184, Length=589, Percent_Identity=41.9354838709677, Blast_Score=488, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322112, Length=657, Percent_Identity=39.5738203957382, Blast_Score=506, Evalue=1e-144,
Organism=Saccharomyces cerevisiae, GI6322654, Length=416, Percent_Identity=37.7403846153846, Blast_Score=297, Evalue=4e-81,
Organism=Drosophila melanogaster, GI24583842, Length=599, Percent_Identity=41.9031719532554, Blast_Score=504, Evalue=1e-143,
Organism=Drosophila melanogaster, GI28574141, Length=599, Percent_Identity=41.9031719532554, Blast_Score=504, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24583839, Length=599, Percent_Identity=41.9031719532554, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 74288; Mature: 74157

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESLIKVQFKEGNTKEFPKGITIKRIAESISTTLRKHAVVAKVDEKFVDLSYTLDKDVK
CCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHEEEEEEECCCCE
LDIFTLDSKEGLSVLRHTSTHILAHAVKRLYSSVSLGIGPVIEDEFFYDLKLEHSLSPED
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHH
LLAIEREMEKIIKENIEIKRIVVSYEEAEKLFEENNEPLKLEILRGIPKGQEITLYQQGE
HHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCC
FIDLCRGPHLPSTGFIKAFKLTRVSGAYWRGNRQKEVLQRVYGVAFKKKDDLNEYLYFLE
EEEHHCCCCCCCCCCEEEEEEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
QAAKHDHRQLGKQLDLFMFSEEAPGMPFYLPKGQIVRKELEKLSRELQNNASYDEVHTPF
HHHHHHHHHHHHHHEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCHHCCCE
MMNYRLWEKSGHWDHYQENMYFSEVDNTKFAIKPMNCPGHMLIFKNNLYSYRDLPIRLAE
EEEEEEECCCCCCCCHHCCCEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEHHH
YGQVHRHEFSGSLNGLLRVRTFCQDDAHIFVREDQIESEIKKVFHLIDQVYRTIGFEYSV
HCHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELSTRPDDSLGNNHLWEVSEGSLKNVLDDLAIKYQVNEGDGAFYGPKIDFHIKDALKRSH
EECCCCCCCCCCCCEEEECCCHHHHHHHCCEEEEEEECCCCCEECCEEEEEEHHHHHCCC
QCGTIQLDFQMPEKFDLTYINHNNEKVRPVVIHRAIFGSIDRFFGILIEHFAGVFPVWLA
CCCEEEEEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIQVQIIPISHIHLEYCLGIKSELKQHGIRVQINESNQKLGYKIREAQVQKIPYTLVVGD
CEEEEEEEECHHHHHHHHCHHHHHHHCCCEEEECCCCCCCCEEHHHHHHHHCCEEEEECC
NEIKENGVNVRKYGEEQQKNVSIESFKKNILQQIKTRSV
CHHHHCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SESLIKVQFKEGNTKEFPKGITIKRIAESISTTLRKHAVVAKVDEKFVDLSYTLDKDVK
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEHHHHHEEEEEEECCCCE
LDIFTLDSKEGLSVLRHTSTHILAHAVKRLYSSVSLGIGPVIEDEFFYDLKLEHSLSPED
EEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCHHH
LLAIEREMEKIIKENIEIKRIVVSYEEAEKLFEENNEPLKLEILRGIPKGQEITLYQQGE
HHHHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHCCCCCEEEEEECCCCCCCEEEEEECCC
FIDLCRGPHLPSTGFIKAFKLTRVSGAYWRGNRQKEVLQRVYGVAFKKKDDLNEYLYFLE
EEEHHCCCCCCCCCCEEEEEEEECCCHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
QAAKHDHRQLGKQLDLFMFSEEAPGMPFYLPKGQIVRKELEKLSRELQNNASYDEVHTPF
HHHHHHHHHHHHHHEEEEEECCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCHHCCCE
MMNYRLWEKSGHWDHYQENMYFSEVDNTKFAIKPMNCPGHMLIFKNNLYSYRDLPIRLAE
EEEEEEECCCCCCCCHHCCCEEEECCCCEEEEEECCCCCEEEEEECCCCCCCCCCEEHHH
YGQVHRHEFSGSLNGLLRVRTFCQDDAHIFVREDQIESEIKKVFHLIDQVYRTIGFEYSV
HCHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHCCCEEEE
ELSTRPDDSLGNNHLWEVSEGSLKNVLDDLAIKYQVNEGDGAFYGPKIDFHIKDALKRSH
EECCCCCCCCCCCCEEEECCCHHHHHHHCCEEEEEEECCCCCEECCEEEEEEHHHHHCCC
QCGTIQLDFQMPEKFDLTYINHNNEKVRPVVIHRAIFGSIDRFFGILIEHFAGVFPVWLA
CCCEEEEEEECCCCEEEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIQVQIIPISHIHLEYCLGIKSELKQHGIRVQINESNQKLGYKIREAQVQKIPYTLVVGD
CEEEEEEEECHHHHHHHHCHHHHHHHCCCEEEECCCCCCCCEEHHHHHHHHCCEEEEECC
NEIKENGVNVRKYGEEQQKNVSIESFKKNILQQIKTRSV
CHHHHCCCCHHHCCCHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2115870; 9384377; 9353933; 1379177 [H]