Click here to switch to the map view.

The map label for this gene is cwlD [H]

Identifier: 52784009

GI number: 52784009

Start: 153780

End: 154493

Strand: Direct

Name: cwlD [H]

Synonym: BLi00171

Alternate gene names: 52784009

Gene position: 153780-154493 (Clockwise)

Preceding gene: 52784008

Following gene: 52784010

Centisome position: 3.64

GC content: 46.22

Gene sequence:

>714_bases
ATGAAGAAAAAAATAAAATGGCTCGGGTTTTTACTCGGCTTTGTCGTTTTATTATGTTTATTTCAATATCAATTCAACAA
TGATGATTCTTGGCGGTCATGGAATCTTCCGCTGAGCGGCAAAATCATTTATATTGATCCTGGACACGGGGGCGCCGATG
GAGGGGCATCAAGCGGTGAACTCCTTGAAAAAGACGTGGCTCTTGAAGTATCCCTAAGAATCAGGGACTATCTCCAGGAG
CAGGGCGCCTTGGTGATGCTGACGCGGGAGGACGATCATGATCTCGCTCCTGAAGAAACGAGGGGACTCAGCAGAAGAAA
AGCTGAAGACTTGCGGAAGAGGGTCGATATGATCAACAATTCTGAAGCCGATCTTTACCTCAGCATCCATTTGAATGCGA
TTCCTTCCGCGCGATGGAGCGGTGCGCAAAGCTTCTATTACGGGCAATATGAAGAGAATGAACGGGCCGCCAAGTTTATT
CAGGATGAATTAAGACATAATCTTGAAAACACGACGCGGAAGGCAAAGCGGATACACGGAATTTATTTGATGCAAAATGT
TAAAAAGCCCGGGGCCCTTGTCGAAATCGGGTTTTTGTCTAACCCGGAAGAGGCAAAACAGCTGGCCAAGCCCAAATATC
AGGACAAAATCGCAGCATCCGTTTATAAAGGCGTATTGCGCTACTTTACAGAAGACAGAGACCCTCCTGAATAA

Upstream 100 bases:

>100_bases
TCAGATAAAACAAAGTTTTATGAAAAAGTGAGGCCAAGCTTAATCATATTTCCTTTCCTTGTCCCATATCTTGTAGTAAG
GACGAGCGGGAGGGAAAGGG

Downstream 100 bases:

>100_bases
GAGGGTTTCTTTTTGTGTCAAAGACACTATAAGGAAAGATATGCTATACTTATTTTGTAAACGAATACAACAAAGGGTGA
GATCAATGTTGCGAGAAGAC

Product: CwlD

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 237; Mature: 237

Protein sequence:

>237_residues
MKKKIKWLGFLLGFVVLLCLFQYQFNNDDSWRSWNLPLSGKIIYIDPGHGGADGGASSGELLEKDVALEVSLRIRDYLQE
QGALVMLTREDDHDLAPEETRGLSRRKAEDLRKRVDMINNSEADLYLSIHLNAIPSARWSGAQSFYYGQYEENERAAKFI
QDELRHNLENTTRKAKRIHGIYLMQNVKKPGALVEIGFLSNPEEAKQLAKPKYQDKIAASVYKGVLRYFTEDRDPPE

Sequences:

>Translated_237_residues
MKKKIKWLGFLLGFVVLLCLFQYQFNNDDSWRSWNLPLSGKIIYIDPGHGGADGGASSGELLEKDVALEVSLRIRDYLQE
QGALVMLTREDDHDLAPEETRGLSRRKAEDLRKRVDMINNSEADLYLSIHLNAIPSARWSGAQSFYYGQYEENERAAKFI
QDELRHNLENTTRKAKRIHGIYLMQNVKKPGALVEIGFLSNPEEAKQLAKPKYQDKIAASVYKGVLRYFTEDRDPPE
>Mature_237_residues
MKKKIKWLGFLLGFVVLLCLFQYQFNNDDSWRSWNLPLSGKIIYIDPGHGGADGGASSGELLEKDVALEVSLRIRDYLQE
QGALVMLTREDDHDLAPEETRGLSRRKAEDLRKRVDMINNSEADLYLSIHLNAIPSARWSGAQSFYYGQYEENERAAKFI
QDELRHNLENTTRKAKRIHGIYLMQNVKKPGALVEIGFLSNPEEAKQLAKPKYQDKIAASVYKGVLRYFTEDRDPPE

Specific function: Cell-Wall Hydrolase Probably Involved In Cell-Wall Hydrolysis, Septation Or Recycling (By Similarity). [C]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=235, Percent_Identity=26.3829787234043, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=24.3589743589744, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508
- InterPro:   IPR014234 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27165; Mature: 27165

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKIKWLGFLLGFVVLLCLFQYQFNNDDSWRSWNLPLSGKIIYIDPGHGGADGGASSGE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCH
LLEKDVALEVSLRIRDYLQEQGALVMLTREDDHDLAPEETRGLSRRKAEDLRKRVDMINN
HHHHHHHEEEHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCHHHHHHHHHHHHHHHHCC
SEADLYLSIHLNAIPSARWSGAQSFYYGQYEENERAAKFIQDELRHNLENTTRKAKRIHG
CCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYLMQNVKKPGALVEIGFLSNPEEAKQLAKPKYQDKIAASVYKGVLRYFTEDRDPPE
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKKIKWLGFLLGFVVLLCLFQYQFNNDDSWRSWNLPLSGKIIYIDPGHGGADGGASSGE
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCEEEEEECCCCCCCCCCCCCH
LLEKDVALEVSLRIRDYLQEQGALVMLTREDDHDLAPEETRGLSRRKAEDLRKRVDMINN
HHHHHHHEEEHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHCHHHHHHHHHHHHHHHHCC
SEADLYLSIHLNAIPSARWSGAQSFYYGQYEENERAAKFIQDELRHNLENTTRKAKRIHG
CCCEEEEEEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IYLMQNVKKPGALVEIGFLSNPEEAKQLAKPKYQDKIAASVYKGVLRYFTEDRDPPE
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7559346; 8969501; 9384377 [H]