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The map label for this gene is tuf [H]

Identifier: 52783969

GI number: 52783969

Start: 131768

End: 132958

Strand: Direct

Name: tuf [H]

Synonym: BLi00131

Alternate gene names: 52783969

Gene position: 131768-132958 (Clockwise)

Preceding gene: 52783968

Following gene: 52783970

Centisome position: 3.12

GC content: 45.51

Gene sequence:

>1191_bases
ATGGCTAAAGAAAAATTCGACCGTTCCAAATCACATGCCAACATTGGTACAATTGGACACGTTGACCATGGTAAAACTAC
ATTAACAGCTGCTATCACTACTGTGCTTCATAAGAAATCTGGTAAAGGTACTGCTATGGCATACGACCAAATCGACGGTG
CTCCAGAAGAGCGCGAGCGCGGTATCACAATTTCTACTGCACACGTTGAGTACGAAACTGACAACCGTCACTATGCACAC
GTTGACTGCCCAGGACACGCTGACTATGTTAAAAACATGATCACTGGTGCTGCGCAAATGGACGGTGCGATCCTTGTTGT
TTCTGCTGCTGACGGCCCAATGCCACAAACTCGTGAGCACATCCTTCTTTCTCGTAACGTAGGTGTGCCTTACATCGTAG
TATTCTTGAACAAATGCGACATGGTAGACGACGAAGAGCTTCTTGAGCTAGTTGAAATGGAAGTTCGCGATCTTCTTTCT
GAGTATGAGTTCCCTGGTGACGATGTACCAGTTATCAAAGGTTCTGCTCTTAAAGCTCTTGAAGGAGATGCTCAGTACGA
AGAAAAAATCTTTGAACTGATGGCTGCTGTTGACGAGTACATCCCAACTCCAGAGCGTGAAACTGACAAGCCATTCATGA
TGCCTGTTGAGGACGTATTCTCAATCACAGGTCGTGGAACAGTTGCTACTGGACGCGTAGAGCGCGGACAAGTTAAAGTC
GGTGACGAAGTTGAAATCATCGGTCTTCAAGAAGAAAACAAGAAAACAACTGTTACAGGTGTTGAAATGTTCCGCAAGCT
TCTTGACTATGCAGAAGCTGGAGACAACATCGGTGCACTTCTTCGCGGTGTATCTCGTGAAGAAATCCAACGCGGTCAAG
TACTTGCTCAACCAGGTACAATCACTCCGCACAAAAAATTCAAAGCTGAAGTTTACGTTCTTTCTAAAGAAGAGGGTGGA
CGTCACACTCCATTCTTCTCTAACTACCGCCCTCAGTTCTACTTCCGTACAACTGACGTAACTGGTATCATTCAGCTTCC
AGAAGGCGTAGAAATGGTTATGCCTGGAGACAACATCGAAATGACTGTTGAATTGATTTCAACAATCGCGATCGAAGACG
GAACTCGCTTCTCTATCCGTGAAGGCGGACGTACAGTTGGTTCTGGCGTTGTTTCTTCAATCATCGAATAA

Upstream 100 bases:

>100_bases
AAAGTATAACTACTATTGTAAGATGAGAAAGTGAAACATTGCTTTCACTTTCTATCACTCTATATAACAAAAACTAACCT
TTTTAAGGAGGATTTTCAGA

Downstream 100 bases:

>100_bases
TACTTATAAAATAAAAAACGAGATCCAGCCGGGTCTCGTTTTTTATTTTGAGCCGCTATGCTAAAGGGATTTATTTTCCG
CCTGTAAGTCCTTGATTAAC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFDRSKSHANIGTIGHVDHGKTTLTAAITTVLHKKSGKGTAMAYDQIDGAPEERERGITISTAHVEYETDNRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRNVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLS
EYEFPGDDVPVIKGSALKALEGDAQYEEKIFELMAAVDEYIPTPERETDKPFMMPVEDVFSITGRGTVATGRVERGQVKV
GDEVEIIGLQEENKKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREEIQRGQVLAQPGTITPHKKFKAEVYVLSKEEGG
RHTPFFSNYRPQFYFRTTDVTGIIQLPEGVEMVMPGDNIEMTVELISTIAIEDGTRFSIREGGRTVGSGVVSSIIE

Sequences:

>Translated_396_residues
MAKEKFDRSKSHANIGTIGHVDHGKTTLTAAITTVLHKKSGKGTAMAYDQIDGAPEERERGITISTAHVEYETDNRHYAH
VDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRNVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLS
EYEFPGDDVPVIKGSALKALEGDAQYEEKIFELMAAVDEYIPTPERETDKPFMMPVEDVFSITGRGTVATGRVERGQVKV
GDEVEIIGLQEENKKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREEIQRGQVLAQPGTITPHKKFKAEVYVLSKEEGG
RHTPFFSNYRPQFYFRTTDVTGIIQLPEGVEMVMPGDNIEMTVELISTIAIEDGTRFSIREGGRTVGSGVVSSIIE
>Mature_395_residues
AKEKFDRSKSHANIGTIGHVDHGKTTLTAAITTVLHKKSGKGTAMAYDQIDGAPEERERGITISTAHVEYETDNRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRNVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSE
YEFPGDDVPVIKGSALKALEGDAQYEEKIFELMAAVDEYIPTPERETDKPFMMPVEDVFSITGRGTVATGRVERGQVKVG
DEVEIIGLQEENKKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREEIQRGQVLAQPGTITPHKKFKAEVYVLSKEEGGR
HTPFFSNYRPQFYFRTTDVTGIIQLPEGVEMVMPGDNIEMTVELISTIAIEDGTRFSIREGGRTVGSGVVSSIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=396, Percent_Identity=55.8080808080808, Blast_Score=460, Evalue=1e-129,
Organism=Homo sapiens, GI223555963, Length=442, Percent_Identity=30.316742081448, Blast_Score=168, Evalue=6e-42,
Organism=Homo sapiens, GI5729864, Length=442, Percent_Identity=30.316742081448, Blast_Score=168, Evalue=8e-42,
Organism=Homo sapiens, GI4503475, Length=354, Percent_Identity=32.4858757062147, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4503471, Length=447, Percent_Identity=29.082774049217, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI194018522, Length=343, Percent_Identity=27.1137026239067, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI194018520, Length=343, Percent_Identity=27.1137026239067, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194097354, Length=343, Percent_Identity=27.1137026239067, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI46094014, Length=330, Percent_Identity=27.5757575757576, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI54607086, Length=327, Percent_Identity=28.7461773700306, Blast_Score=117, Evalue=3e-26,
Organism=Homo sapiens, GI4503507, Length=342, Percent_Identity=28.6549707602339, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI157426893, Length=254, Percent_Identity=27.1653543307087, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=75.9493670886076, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=75.9493670886076, Blast_Score=605, Evalue=1e-174,
Organism=Escherichia coli, GI2367247, Length=374, Percent_Identity=30.2139037433155, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI48994988, Length=273, Percent_Identity=32.967032967033, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789108, Length=261, Percent_Identity=34.8659003831418, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788922, Length=150, Percent_Identity=32.6666666666667, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=51.4925373134328, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=43.9276485788114, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI17552884, Length=456, Percent_Identity=32.2368421052632, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17569207, Length=456, Percent_Identity=32.2368421052632, Blast_Score=167, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI32566303, Length=432, Percent_Identity=29.6296296296296, Blast_Score=144, Evalue=9e-35,
Organism=Caenorhabditis elegans, GI115532067, Length=427, Percent_Identity=28.8056206088993, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI115532065, Length=427, Percent_Identity=28.8056206088993, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI32566629, Length=345, Percent_Identity=29.8550724637681, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17506081, Length=298, Percent_Identity=28.8590604026846, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.3116883116883, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=282, Percent_Identity=26.5957446808511, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=232, Percent_Identity=28.448275862069, Blast_Score=78, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=65.4911838790932, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=29.954954954955, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=29.954954954955, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6322937, Length=346, Percent_Identity=24.8554913294798, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.023598820059, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=306, Percent_Identity=27.1241830065359, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=242, Percent_Identity=29.3388429752066, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=178, Percent_Identity=30.8988764044944, Blast_Score=70, Evalue=6e-13,
Organism=Drosophila melanogaster, GI281363316, Length=397, Percent_Identity=56.1712846347607, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17864358, Length=397, Percent_Identity=56.1712846347607, Blast_Score=457, Evalue=1e-129,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=47.5452196382429, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI45553807, Length=454, Percent_Identity=29.9559471365639, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI45553816, Length=454, Percent_Identity=29.9559471365639, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24651721, Length=454, Percent_Identity=29.9559471365639, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI17864154, Length=454, Percent_Identity=29.9559471365639, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI24652838, Length=447, Percent_Identity=29.3064876957494, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137572, Length=447, Percent_Identity=29.3064876957494, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=32.6732673267327, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI45550900, Length=343, Percent_Identity=31.1953352769679, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=29.6511627906977, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24647046, Length=368, Percent_Identity=26.6304347826087, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24647048, Length=368, Percent_Identity=26.6304347826087, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI28572034, Length=164, Percent_Identity=35.9756097560976, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43654; Mature: 43523

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFDRSKSHANIGTIGHVDHGKTTLTAAITTVLHKKSGKGTAMAYDQIDGAPEERER
CCCCHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHC
GITISTAHVEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREH
CCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHH
ILLSRNVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYEFPGDDVPVIKGSALKAL
EEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHH
EGDAQYEEKIFELMAAVDEYIPTPERETDKPFMMPVEDVFSITGRGTVATGRVERGQVKV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCEEEE
GDEVEIIGLQEENKKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREEIQRGQVLAQPGT
CCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCCEEECCCC
ITPHKKFKAEVYVLSKEEGGRHTPFFSNYRPQFYFRTTDVTGIIQLPEGVEMVMPGDNIE
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MTVELISTIAIEDGTRFSIREGGRTVGSGVVSSIIE
EHEEEHHHHEECCCCEEEECCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
AKEKFDRSKSHANIGTIGHVDHGKTTLTAAITTVLHKKSGKGTAMAYDQIDGAPEERER
CCCHHCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHC
GITISTAHVEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREH
CCEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHH
ILLSRNVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLSEYEFPGDDVPVIKGSALKAL
EEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCHHHH
EGDAQYEEKIFELMAAVDEYIPTPERETDKPFMMPVEDVFSITGRGTVATGRVERGQVKV
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCCEEEE
GDEVEIIGLQEENKKTTVTGVEMFRKLLDYAEAGDNIGALLRGVSREEIQRGQVLAQPGT
CCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHCCCEEECCCC
ITPHKKFKAEVYVLSKEEGGRHTPFFSNYRPQFYFRTTDVTGIIQLPEGVEMVMPGDNIE
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCEEEECCCCCE
MTVELISTIAIEDGTRFSIREGGRTVGSGVVSSIIE
EHEEEHHHHEECCCCEEEECCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA