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The map label for this gene is lipW [H]

Identifier: 52144489

GI number: 52144489

Start: 835044

End: 835985

Strand: Reverse

Name: lipW [H]

Synonym: BCZK0735

Alternate gene names: 52144489

Gene position: 835985-835044 (Counterclockwise)

Preceding gene: 52144487

Following gene: 52144492

Centisome position: 15.77

GC content: 39.81

Gene sequence:

>942_bases
ATGAAGAACCGAGTAAACCCAGCATTATTACCAGGATTAGAAATGTATCCAGATCAAGATTTACGCCCAGAGTGTTTGCC
AGTAATGAGAGAAGAAGCATCAAAAGTCTTCCCTCCTACCATTGTTGATGAAACTCTTTCTGTAACAAATGAAGTCATTG
AGGGACCTGATGCAAATCCATTACGATTAAAAATTTATCGTCCAAAATCAAATAATGAATCCTTACCTGCTCTGTTGTGG
ATACATGGTGGCGGGTATATCTTGGGATCTATAAATGATAACGATGATCTTGCTGTAAAAATTGCAAAAGAGGCTAACTG
TATGGTCGTTTCTGTAGATTACCGTTTAGCTCCTGAACATCCTTATCCAGCTCCAATTGAAGATTGTTATGCAGCATTAA
AATGGATTGCTGATAACGCGGAGACATTGAACATTGATTCGAATCGAATTGGTGTTGCAGGAGTGAGCGCTGGTGGCGGA
CTAACAGCTGCATTAACATTATTAGCCCGTGATCGCCAATACCCTTCTATTTGTTTCCAAATGCCGCTTTATCCAATGAT
TGATGATCGTAATAACACACCTTCTGCCAATGAAATTAAAGAAGGATTTGTTTGGAATCAAAAATCAAATGAAGCTGGCT
GGAAAATGTATTTAGGAGAATTACATGGAACGGATCATATTCCCGCTTATGCAGCCGCTGCTCGAGCAGAAGATTATCGT
AATTTACCACGTACCTACACATGCGTTGGTCAATTGGACCCATTCCGTAGTGAAACATTAACCTATGTAACCAAACTTGC
AGAAGCTGGTGTTGATGTCGAGTTCCATTTATATCCTGGGGCTTATCATGGGTTCGAAATGATGCATCCGGATACTGATA
TATCTATTCACGCTTGGAATGATTATGTACGAGCAGTTAAAACTGGTTTCGCAAGAAAGTAA

Upstream 100 bases:

>100_bases
CTCTAATGCCTATGATAAATTGCTATACATAGGTAGCTTACAATTATATTTCTAAAATAATATTTCACCTTTCATAAAAA
ATAAAAAAGGAGTTTTTAAA

Downstream 100 bases:

>100_bases
CTAAGGTAGCGACAAAAAATTTCATAATAAAGGAGAATTTTTAATGAAGAATAGAGTTCATCCAGCATTAAGAGAAATGT
TTTCTTTATTATCAGAAGTA

Product: esterase

Products: NA

Alternate protein names: Triacylglycerol lipase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKNRVNPALLPGLEMYPDQDLRPECLPVMREEASKVFPPTIVDETLSVTNEVIEGPDANPLRLKIYRPKSNNESLPALLW
IHGGGYILGSINDNDDLAVKIAKEANCMVVSVDYRLAPEHPYPAPIEDCYAALKWIADNAETLNIDSNRIGVAGVSAGGG
LTAALTLLARDRQYPSICFQMPLYPMIDDRNNTPSANEIKEGFVWNQKSNEAGWKMYLGELHGTDHIPAYAAAARAEDYR
NLPRTYTCVGQLDPFRSETLTYVTKLAEAGVDVEFHLYPGAYHGFEMMHPDTDISIHAWNDYVRAVKTGFARK

Sequences:

>Translated_313_residues
MKNRVNPALLPGLEMYPDQDLRPECLPVMREEASKVFPPTIVDETLSVTNEVIEGPDANPLRLKIYRPKSNNESLPALLW
IHGGGYILGSINDNDDLAVKIAKEANCMVVSVDYRLAPEHPYPAPIEDCYAALKWIADNAETLNIDSNRIGVAGVSAGGG
LTAALTLLARDRQYPSICFQMPLYPMIDDRNNTPSANEIKEGFVWNQKSNEAGWKMYLGELHGTDHIPAYAAAARAEDYR
NLPRTYTCVGQLDPFRSETLTYVTKLAEAGVDVEFHLYPGAYHGFEMMHPDTDISIHAWNDYVRAVKTGFARK
>Mature_313_residues
MKNRVNPALLPGLEMYPDQDLRPECLPVMREEASKVFPPTIVDETLSVTNEVIEGPDANPLRLKIYRPKSNNESLPALLW
IHGGGYILGSINDNDDLAVKIAKEANCMVVSVDYRLAPEHPYPAPIEDCYAALKWIADNAETLNIDSNRIGVAGVSAGGG
LTAALTLLARDRQYPSICFQMPLYPMIDDRNNTPSANEIKEGFVWNQKSNEAGWKMYLGELHGTDHIPAYAAAARAEDYR
NLPRTYTCVGQLDPFRSETLTYVTKLAEAGVDVEFHLYPGAYHGFEMMHPDTDISIHAWNDYVRAVKTGFARK

Specific function: Has An Esterase Activity. Triacetyl Glycerol (Triacetin) Is A Substrate Of The Enzyme. [C]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=334, Percent_Identity=28.1437125748503, Blast_Score=96, Evalue=6e-20,
Organism=Homo sapiens, GI206597554, Length=327, Percent_Identity=28.1345565749235, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI157041239, Length=280, Percent_Identity=24.2857142857143, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI68051721, Length=324, Percent_Identity=24.0740740740741, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI61966717, Length=282, Percent_Identity=24.113475177305, Blast_Score=69, Evalue=7e-12,
Organism=Homo sapiens, GI21328446, Length=103, Percent_Identity=33.0097087378641, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786682, Length=230, Percent_Identity=28.695652173913, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17540028, Length=136, Percent_Identity=34.5588235294118, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI72001146, Length=133, Percent_Identity=34.5864661654135, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71996133, Length=132, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=293, Percent_Identity=24.9146757679181, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533410, Length=90, Percent_Identity=38.8888888888889, Blast_Score=71, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI115533412, Length=90, Percent_Identity=38.8888888888889, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24656084, Length=93, Percent_Identity=35.4838709677419, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24656076, Length=93, Percent_Identity=35.4838709677419, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20130169, Length=93, Percent_Identity=35.4838709677419, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: =3.1.1.3 [H]

Molecular weight: Translated: 34857; Mature: 34857

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRVNPALLPGLEMYPDQDLRPECLPVMREEASKVFPPTIVDETLSVTNEVIEGPDANP
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
LRLKIYRPKSNNESLPALLWIHGGGYILGSINDNDDLAVKIAKEANCMVVSVDYRLAPEH
EEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCCEEEEEEEEEECCCC
PYPAPIEDCYAALKWIADNAETLNIDSNRIGVAGVSAGGGLTAALTLLARDRQYPSICFQ
CCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEE
MPLYPMIDDRNNTPSANEIKEGFVWNQKSNEAGWKMYLGELHGTDHIPAYAAAARAEDYR
CCCCEEECCCCCCCCHHHHHHCCEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
NLPRTYTCVGQLDPFRSETLTYVTKLAEAGVDVEFHLYPGAYHGFEMMHPDTDISIHAWN
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCEEEEEHH
DYVRAVKTGFARK
HHHHHHHHHCCCC
>Mature Secondary Structure
MKNRVNPALLPGLEMYPDQDLRPECLPVMREEASKVFPPTIVDETLSVTNEVIEGPDANP
CCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
LRLKIYRPKSNNESLPALLWIHGGGYILGSINDNDDLAVKIAKEANCMVVSVDYRLAPEH
EEEEEECCCCCCCCCCEEEEEECCCEEEEECCCCCCEEEEEECCCCEEEEEEEEEECCCC
PYPAPIEDCYAALKWIADNAETLNIDSNRIGVAGVSAGGGLTAALTLLARDRQYPSICFQ
CCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEE
MPLYPMIDDRNNTPSANEIKEGFVWNQKSNEAGWKMYLGELHGTDHIPAYAAAARAEDYR
CCCCEEECCCCCCCCHHHHHHCCEECCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHH
NLPRTYTCVGQLDPFRSETLTYVTKLAEAGVDVEFHLYPGAYHGFEMMHPDTDISIHAWN
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEECCCCCEEEEEHH
DYVRAVKTGFARK
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1907455 [H]