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The map label for this gene is yisK [H]

Identifier: 52144180

GI number: 52144180

Start: 1154112

End: 1155020

Strand: Direct

Name: yisK [H]

Synonym: BCZK1048

Alternate gene names: 52144180

Gene position: 1154112-1155020 (Clockwise)

Preceding gene: 52144187

Following gene: 52144176

Centisome position: 21.77

GC content: 36.63

Gene sequence:

>909_bases
GTGGAAATATTGCGTTTGGTAACGGCGAAAAAAGAAGAAAAGGTATTTGTAGGTATTGTGGATGAAGAGGAAGAAAAGGT
ATTGCACTTAAGAGAAGCGCAAAGGCAAAAGGGAGAAAAGGTTACAATCCCCATTACAATGTTAGAGTGTATTGAAAGAG
GAACTGAATGTTTTGAAAAAATATGTGATATTGTAAATTGGGCGAAGGAAAATGAAGGGGCGGCGTATTATCCGTTAACT
GAAGTGAAAATATTAGCTCCAATTCCAAGGCCAAGAAAAAATATTTTGTGCGTTGGAAAAAACTATCGTGAGCATGCGAT
AGAAATGGGCGGAGTAGAGTCAATTCCAGAAAATATAATGATCTTTACGAAAGCGCCAACGACAGTTATTGGAATGGATG
AAAAAATTAATAGTCATCCCCACGCAACAAATGAATTAGACTATGAAGGAGAACTAGCTATTGTCATTGGTAAGCGAGGG
AAACAAATAAAAAAAGAAAAAGCGCTTGAGCATGTTTTTGGTTATACCATTATAAATGATATAACTGCTCGCGACATTCA
AAGGAAGCATAAACAATTTTTCCTTGGAAAAAGTTTCGATACATTTTGTCCGATGGGACCGTATTTAATTCATAAATCGA
TAGTTAGTACGCCAAATGCGTTACACATTGAAACGATAGTAAATGGGGAAGTAAGGCAAACTTCAAATACAAATAAAATG
ATTTTTTCAATAGAAGAAATCATTTCAACGATAAGTAAAGGAATGACATTAGAACCTGGAGATATTATCGCAACAGGAAC
CCCGGCTGGTGTCGGAAAAGGTTTCACGCCACCAAAGTTTTTACATGCGGGTGATGAAGTGGTAATTACGGTAGAAGGAA
TTGGTACTTTGCGAAATGTAGTGAAGTGA

Upstream 100 bases:

>100_bases
ATAGGATATGAGAAGAATATTGTAAAAATTAGCGTGTATGAGAAGGTTTTTTTCTTACGGATGCGAATACAATTAAAGGG
TATGAGTTTTAGGATGGAGA

Downstream 100 bases:

>100_bases
AGAAAAGAGCTATCTTATTAAGATAGCTCTTTTTTATGGCCATGGGTTATTGAAAAAATCTAAAATAGCAACAATATATA
ATCCGATGAGTATTAATGTA

Product: fumarylacetoacetate hydrolase family protein

Products: acetoacetate; fumarate

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MEILRLVTAKKEEKVFVGIVDEEEEKVLHLREAQRQKGEKVTIPITMLECIERGTECFEKICDIVNWAKENEGAAYYPLT
EVKILAPIPRPRKNILCVGKNYREHAIEMGGVESIPENIMIFTKAPTTVIGMDEKINSHPHATNELDYEGELAIVIGKRG
KQIKKEKALEHVFGYTIINDITARDIQRKHKQFFLGKSFDTFCPMGPYLIHKSIVSTPNALHIETIVNGEVRQTSNTNKM
IFSIEEIISTISKGMTLEPGDIIATGTPAGVGKGFTPPKFLHAGDEVVITVEGIGTLRNVVK

Sequences:

>Translated_302_residues
MEILRLVTAKKEEKVFVGIVDEEEEKVLHLREAQRQKGEKVTIPITMLECIERGTECFEKICDIVNWAKENEGAAYYPLT
EVKILAPIPRPRKNILCVGKNYREHAIEMGGVESIPENIMIFTKAPTTVIGMDEKINSHPHATNELDYEGELAIVIGKRG
KQIKKEKALEHVFGYTIINDITARDIQRKHKQFFLGKSFDTFCPMGPYLIHKSIVSTPNALHIETIVNGEVRQTSNTNKM
IFSIEEIISTISKGMTLEPGDIIATGTPAGVGKGFTPPKFLHAGDEVVITVEGIGTLRNVVK
>Mature_302_residues
MEILRLVTAKKEEKVFVGIVDEEEEKVLHLREAQRQKGEKVTIPITMLECIERGTECFEKICDIVNWAKENEGAAYYPLT
EVKILAPIPRPRKNILCVGKNYREHAIEMGGVESIPENIMIFTKAPTTVIGMDEKINSHPHATNELDYEGELAIVIGKRG
KQIKKEKALEHVFGYTIINDITARDIQRKHKQFFLGKSFDTFCPMGPYLIHKSIVSTPNALHIETIVNGEVRQTSNTNKM
IFSIEEIISTISKGMTLEPGDIIATGTPAGVGKGFTPPKFLHAGDEVVITVEGIGTLRNVVK

Specific function: Unknown

COG id: COG0179

COG function: function code Q; 2-keto-4-pentenoate hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase (catechol pathway)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAH family [H]

Homologues:

Organism=Homo sapiens, GI156231349, Length=262, Percent_Identity=42.7480916030534, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI40786394, Length=262, Percent_Identity=42.3664122137405, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI215422413, Length=185, Percent_Identity=42.7027027027027, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI13654274, Length=185, Percent_Identity=42.7027027027027, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI66348062, Length=185, Percent_Identity=42.7027027027027, Blast_Score=130, Evalue=1e-30,
Organism=Escherichia coli, GI1787428, Length=212, Percent_Identity=33.0188679245283, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557057, Length=183, Percent_Identity=39.8907103825137, Blast_Score=130, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6324161, Length=229, Percent_Identity=29.2576419213974, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28572127, Length=221, Percent_Identity=42.0814479638009, Blast_Score=184, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24663695, Length=245, Percent_Identity=37.9591836734694, Blast_Score=153, Evalue=1e-37,
Organism=Drosophila melanogaster, GI28571789, Length=183, Percent_Identity=34.4262295081967, Blast_Score=94, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 3.7.1.2

Molecular weight: Translated: 33737; Mature: 33737

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEILRLVTAKKEEKVFVGIVDEEEEKVLHLREAQRQKGEKVTIPITMLECIERGTECFEK
CCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHH
ICDIVNWAKENEGAAYYPLTEVKILAPIPRPRKNILCVGKNYREHAIEMGGVESIPENIM
HHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCEE
IFTKAPTTVIGMDEKINSHPHATNELDYEGELAIVIGKRGKQIKKEKALEHVFGYTIIND
EEEECCCEEEECCHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ITARDIQRKHKQFFLGKSFDTFCPMGPYLIHKSIVSTPNALHIETIVNGEVRQTSNTNKM
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCE
IFSIEEIISTISKGMTLEPGDIIATGTPAGVGKGFTPPKFLHAGDEVVITVEGIGTLRNV
EEEHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCCCHHHCCCCCEEEEEEECCHHHHHH
VK
CC
>Mature Secondary Structure
MEILRLVTAKKEEKVFVGIVDEEEEKVLHLREAQRQKGEKVTIPITMLECIERGTECFEK
CCHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHH
ICDIVNWAKENEGAAYYPLTEVKILAPIPRPRKNILCVGKNYREHAIEMGGVESIPENIM
HHHHHHHHHCCCCCEECCCEEEEEEECCCCCCCCEEEECCCHHHHHHHCCCCCCCCCCEE
IFTKAPTTVIGMDEKINSHPHATNELDYEGELAIVIGKRGKQIKKEKALEHVFGYTIIND
EEEECCCEEEECCHHCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ITARDIQRKHKQFFLGKSFDTFCPMGPYLIHKSIVSTPNALHIETIVNGEVRQTSNTNKM
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEECCCEEECCCCCCE
IFSIEEIISTISKGMTLEPGDIIATGTPAGVGKGFTPPKFLHAGDEVVITVEGIGTLRNV
EEEHHHHHHHHHCCCEECCCCEEEECCCCCCCCCCCCHHHCCCCCEEEEEEECCHHHHHH
VK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-fumarylacetoacetate; H2O

Specific reaction: 4-fumarylacetoacetate + H2O = acetoacetate + fumarate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377 [H]