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The map label for this gene is nrgA [H]

Identifier: 52144171

GI number: 52144171

Start: 1162797

End: 1164029

Strand: Reverse

Name: nrgA [H]

Synonym: BCZK1056

Alternate gene names: 52144171

Gene position: 1164029-1162797 (Counterclockwise)

Preceding gene: 52144167

Following gene: 52144175

Centisome position: 21.96

GC content: 43.47

Gene sequence:

>1233_bases
ATGAATATGGGAGATACGGTTTTTATGTTTGTAGCGACAGTAATGGTCATGTTAATGACACCAGGATTAGCACTTTTTTA
TGGAGGGATGGTTCGCAGTAAAAACGTACTCAGTACGACAATGCATAGTTATAGTGCAATGGCTATCGTTTCGATTCAAT
GGATTGTAATTGGTTATTCTTTATCATTCGGACCAGATTGGCACGGACTTATCGGCACACTCGATTGGTTCGGTTTAAAT
GGAGTTACCTACGCACCAAATCCAGACTACTCATCTACTATTCCTCACAATTTATTTATGATGTTTCAACTCATGTTCGC
TATTTTAACTCCTGCTTTAATTTCAGGTGCATTTGCCGAAAGAATGCGATTTTCAGCTTTCCTTATTTTCATCCTTCTAT
GGACAACGATCGTTTACAATCCTGTTGCTCATTGGGTATGGGGCGTTGGCGGATGGCTAAGAGAACTTGGCGCGTTAGAC
TTCGCTGGCGGTAATGTCGTTCATATTACATCTGGTGTGGCTGGCCTCGTATTAGCTATTTTCCTTGGAAAACGAAAAAA
CATAAACGGTACTTCTCCTCATCATTTACCATTTACAATGCTAGGCGCTGGGCTGCTATGGTTTGGTTGGTTCGGTTTTA
ACGTCGGAAGCGCATTATCTTTAAATGATGTAGCTTTAACTGCATTTATTAATACGAATATAGCCGCTGCTGCCTCCGCA
TTAACTTGGATGCTTTCAGAGTGGTTCTTTCAATCAAAACCGACTGCGATGGGAGCTGCTTGTGGCGCCGTTTCCGGTCT
AGTCGCTATTACACCAGCTTGCGGGTTCGTTACACCTTTCTCCGCTCTTCTTATCGGAGCGATTGGCGGCGTATTATGCT
TCGGAGCAGTCTTTTTCTTAAAAACAAAATTCGGATACGACGATACACTCGATGCATTCGGATGTCACGGCATCGGCGGA
ACTTGGGGCGGTATTGCGACAGGACTATTTGCAACTACTACCGTAAACGGCGATGGCGCTAACGGATTATTTTACGGAAA
CGCCGCGTTGCTGTTTAAACAACTTGTAGCAATCGGCGCAACATATGCATTCACAATTATAATGACGTACGCCATTATTA
AAGCAATTAACTTCTTCCTCCCTGTCCGGGTCGATGAGCACGAGGAACATATGGGACTTGATATTTCGATGCACGGAGAG
AAAGCTTATGAGTATACTGAGCGAGTGAACTAA

Upstream 100 bases:

>100_bases
AATCTTCTGAATTTTTAGTTGATTTTTCTTTTCACTTCGAATATGATATGAAACATAACAAATCGATGTTATTTTTTCTA
ACATTACAGGAGGAGAGAGA

Downstream 100 bases:

>100_bases
AAAAGAGATGCAATCTATTGATAGTAGATTGCATCTCTTTTTTTGCATTTGTCGGCACAGCATACCCCAATCGGTATTCT
TGTAAATATATCAGCGATTC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN

Sequences:

>Translated_410_residues
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN
>Mature_410_residues
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYSLSFGPDWHGLIGTLDWFGLN
GVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAERMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALD
FAGGNVVHITSGVAGLVLAIFLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFLKTKFGYDDTLDAFGCHGIGG
TWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGATYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGE
KAYEYTERVN

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=407, Percent_Identity=42.7518427518428, Blast_Score=305, Evalue=3e-84,
Organism=Caenorhabditis elegans, GI133931100, Length=429, Percent_Identity=29.1375291375291, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI32564042, Length=423, Percent_Identity=28.1323877068558, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17550182, Length=442, Percent_Identity=27.8280542986425, Blast_Score=131, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=27.6887871853547, Blast_Score=129, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6324187, Length=423, Percent_Identity=40.1891252955083, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6325396, Length=412, Percent_Identity=36.6504854368932, Blast_Score=235, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=36.6028708133971, Blast_Score=228, Evalue=2e-60,
Organism=Drosophila melanogaster, GI161078314, Length=416, Percent_Identity=30.2884615384615, Blast_Score=149, Evalue=4e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 44114; Mature: 44114

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEE
LSFGPDWHGLIGTLDWFGLNGVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHH
FLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHEEEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAYEYTERVN
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCEEEECCCHHHHHHHCCC
>Mature Secondary Structure
MNMGDTVFMFVATVMVMLMTPGLALFYGGMVRSKNVLSTTMHSYSAMAIVSIQWIVIGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHCCHHHCCHHHHHHHHHHHHHHHEEEEEEEEEEE
LSFGPDWHGLIGTLDWFGLNGVTYAPNPDYSSTIPHNLFMMFQLMFAILTPALISGAFAE
ECCCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RMRFSAFLIFILLWTTIVYNPVAHWVWGVGGWLRELGALDFAGGNVVHITSGVAGLVLAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHH
FLGKRKNINGTSPHHLPFTMLGAGLLWFGWFGFNVGSALSLNDVALTAFINTNIAAAASA
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCEEEEEEECCCHHHHHHH
LTWMLSEWFFQSKPTAMGAACGAVSGLVAITPACGFVTPFSALLIGAIGGVLCFGAVFFL
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KTKFGYDDTLDAFGCHGIGGTWGGIATGLFATTTVNGDGANGLFYGNAALLFKQLVAIGA
HHHCCCCCCCHHCCCCCCCCCHHHHHHHHHEEEEECCCCCCCEEECCHHHHHHHHHHHHH
TYAFTIIMTYAIIKAINFFLPVRVDEHEEHMGLDISMHGEKAYEYTERVN
HHHHHHHHHHHHHHHHHHHCCEEECCCHHHCCCEEEECCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]