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The map label for this gene is malS [C]

Identifier: 52144170

GI number: 52144170

Start: 1164242

End: 1165543

Strand: Direct

Name: malS [C]

Synonym: BCZK1057

Alternate gene names: 52144170

Gene position: 1164242-1165543 (Clockwise)

Preceding gene: 52144172

Following gene: 52144169

Centisome position: 21.96

GC content: 33.56

Gene sequence:

>1302_bases
ATGCGTGTGGGGAAAATACATACTAGGAAACTATTCATTTGTTTTTGTTTAGCTGTCGTTTTGTTTGTACCAATACATAC
ATTTGCAGACGAAAAAAGAGAGTGGCGAGATGAAGTTATATATTCCATTATGATTGATCGTTTTAATAATGGGGAACCGA
AAAATGACAAACAGTTAGAAGTTGGTAATTTAGAAGGATATCAGGGCGGAGATATAAGAGGCATTATAAAAAGACTGGAT
TACATAAAAGAAATGGGATTTACCACTGTTATGCTTTCGCCGCTGTTTGAAAGTGTAAAATACGATGGAGTAGACGTGCG
CAATTTTCAGAAGGTAAATGAACATTTTGGAACAGAAAATGATGTAAAAGAACTTGTGCAAGAAGCTCATGCAAAAGGAA
TGAAAGTTATATTTCAATTTCCGCTTGGAGAAAACGAACAACAAGTAATCGACTCGATGAAATGGTGGGTCAAAGAAGTT
GATTTAGATGCAAGTTATGTAATGCATAGTGAAAAAAAGTCTCCTGCTTTTTGGGATGATGTGCAAAAAGATATGCAAGT
GATAAAAAAAGATTTTCGAGTTATGACAAAAGAAGATAGTGAATACAACGAAAAAATAGTAGAATCGTTTTCTAAAGCGG
ACGTATCGGTAAAATCTTTATATGATGTGAGTAAAAAAGACGAGGAATTCATTACATTTTTAGATAATCAAGATACAAAA
AGATTTGCTCGTATTGCAAAGGAAAATATGAATTATCCGCCATCGCGTTTGAAGCTAGCTCTTACATATTTATTGACATC
ACCAGGCATTCCGAATTTTTATTACGGGACTGAAATTGCATTAGATGGAGGGGATACGCCAGATAATAGACGATTAATGG
ATTTCAAATCGGATGAAAAGTTTATGCAGCATATAACAAAACTTGGTGAACTTAGACAAACGAGACCATCTTTACGACGC
GGTACATTTGAACTCTTATACGACAAAAATGGAATGAGTGTACTAAAACGAAAGTATAAAGGTGAAGTCACATTAGTAGC
GATTAATAATACGAAAGAGACGCAAAAAGTTGCTTTACCTGCAAGTACGATTGGTGAAAAACAAGAGTTAAGAGGATTGT
TAGAAGATGAAATTATAAGAGAAGAAAATGGAAAGTTTTATCTCGTTTTAAAGCGTGAAGAATCAAATGTGTATAAAGTT
AATAGAGAAACAGGTGTGAATTGGTTATTTATCTCCTTAATAGTTGGTGTGAACGTATTATTTATTACTTTTTTAATTGC
GGTTAAAAAGAGACGGAAATGA

Upstream 100 bases:

>100_bases
TCAGAAAATCTGACATGTATTAATTTAATAAGAGTGAAATCAACATATGCGATTCGTTCGAATAATTGGTATAATATTCT
TACAATTAAAAACGTGGGGG

Downstream 100 bases:

>100_bases
GTAACAATTTTCTTTAGTAGCTCGTCTTCAGTTTAAGGTAAGGTGTGCTCTTAAAAACAGGTTAAAGAGAAGACTTTCTC
TTTAACCTGTTTTTCTGTGA

Product: alpha-amylase family protein

Products: NA

Alternate protein names: CDase; Cyclomaltodextrin hydrolase, decycling [H]

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLD
YIKEMGFTTVMLSPLFESVKYDGVDVRNFQKVNEHFGTENDVKELVQEAHAKGMKVIFQFPLGENEQQVIDSMKWWVKEV
DLDASYVMHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVESFSKADVSVKSLYDVSKKDEEFITFLDNQDTK
RFARIAKENMNYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGDTPDNRRLMDFKSDEKFMQHITKLGELRQTRPSLRR
GTFELLYDKNGMSVLKRKYKGEVTLVAINNTKETQKVALPASTIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKV
NRETGVNWLFISLIVGVNVLFITFLIAVKKRRK

Sequences:

>Translated_433_residues
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLD
YIKEMGFTTVMLSPLFESVKYDGVDVRNFQKVNEHFGTENDVKELVQEAHAKGMKVIFQFPLGENEQQVIDSMKWWVKEV
DLDASYVMHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVESFSKADVSVKSLYDVSKKDEEFITFLDNQDTK
RFARIAKENMNYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGDTPDNRRLMDFKSDEKFMQHITKLGELRQTRPSLRR
GTFELLYDKNGMSVLKRKYKGEVTLVAINNTKETQKVALPASTIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKV
NRETGVNWLFISLIVGVNVLFITFLIAVKKRRK
>Mature_433_residues
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLEVGNLEGYQGGDIRGIIKRLD
YIKEMGFTTVMLSPLFESVKYDGVDVRNFQKVNEHFGTENDVKELVQEAHAKGMKVIFQFPLGENEQQVIDSMKWWVKEV
DLDASYVMHSEKKSPAFWDDVQKDMQVIKKDFRVMTKEDSEYNEKIVESFSKADVSVKSLYDVSKKDEEFITFLDNQDTK
RFARIAKENMNYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGDTPDNRRLMDFKSDEKFMQHITKLGELRQTRPSLRR
GTFELLYDKNGMSVLKRKYKGEVTLVAINNTKETQKVALPASTIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKV
NRETGVNWLFISLIVGVNVLFITFLIAVKKRRK

Specific function: Hydrolyzes cyclodextrins. Can also act on linear maltodextrins, with the exception of maltose [H]

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789995, Length=128, Percent_Identity=27.34375, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786604, Length=154, Percent_Identity=33.1168831168831, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790687, Length=105, Percent_Identity=31.4285714285714, Blast_Score=66, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322245, Length=112, Percent_Identity=31.25, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6324416, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322241, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322021, Length=108, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6321731, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319776, Length=108, Percent_Identity=32.4074074074074, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321726, Length=108, Percent_Identity=32.4074074074074, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24583745, Length=162, Percent_Identity=30.8641975308642, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221330053, Length=147, Percent_Identity=30.6122448979592, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24586587, Length=72, Percent_Identity=44.4444444444444, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI45549022, Length=179, Percent_Identity=26.2569832402235, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004185
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02903 Alpha-amylase_N [H]

EC number: =3.2.1.54 [H]

Molecular weight: Translated: 50424; Mature: 50424

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VGNLEGYQGGDIRGIIKRLDYIKEMGFTTVMLSPLFESVKYDGVDVRNFQKVNEHFGTEN
ECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
DVKELVQEAHAKGMKVIFQFPLGENEQQVIDSMKWWVKEVDLDASYVMHSEKKSPAFWDD
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHH
VQKDMQVIKKDFRVMTKEDSEYNEKIVESFSKADVSVKSLYDVSKKDEEFITFLDNQDTK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHEEECCCCCHH
RFARIAKENMNYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGDTPDNRRLMDFKSDEK
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEECCCCCHHH
FMQHITKLGELRQTRPSLRRGTFELLYDKNGMSVLKRKYKGEVTLVAINNTKETQKVALP
HHHHHHHHHHHHHHCHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCEEECC
ASTIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKVNRETGVNWLFISLIVGVNVL
HHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
FITFLIAVKKRRK
HHHHHHHHHHCCC
>Mature Secondary Structure
MRVGKIHTRKLFICFCLAVVLFVPIHTFADEKREWRDEVIYSIMIDRFNNGEPKNDKQLE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
VGNLEGYQGGDIRGIIKRLDYIKEMGFTTVMLSPLFESVKYDGVDVRNFQKVNEHFGTEN
ECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHH
DVKELVQEAHAKGMKVIFQFPLGENEQQVIDSMKWWVKEVDLDASYVMHSEKKSPAFWDD
HHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHH
VQKDMQVIKKDFRVMTKEDSEYNEKIVESFSKADVSVKSLYDVSKKDEEFITFLDNQDTK
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHEEECCCCCHH
RFARIAKENMNYPPSRLKLALTYLLTSPGIPNFYYGTEIALDGGDTPDNRRLMDFKSDEK
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCEECCCCCHHH
FMQHITKLGELRQTRPSLRRGTFELLYDKNGMSVLKRKYKGEVTLVAINNTKETQKVALP
HHHHHHHHHHHHHHCHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCCEEECC
ASTIGEKQELRGLLEDEIIREENGKFYLVLKREESNVYKVNRETGVNWLFISLIVGVNVL
HHHCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHH
FITFLIAVKKRRK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1644767; 8425614 [H]