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The map label for this gene is glcF [H]

Identifier: 52144040

GI number: 52144040

Start: 1290221

End: 1291549

Strand: Reverse

Name: glcF [H]

Synonym: BCZK1189

Alternate gene names: 52144040

Gene position: 1291549-1290221 (Counterclockwise)

Preceding gene: 52144038

Following gene: 52144042

Centisome position: 24.36

GC content: 37.62

Gene sequence:

>1329_bases
ATGACAACATTAAATAAAGAAAACATTCAAAAAGAATTTAAAGAACGATTAAGTGAAAATGAACTACTAAACTGTATGCG
ATGCGGTTTCTGTTTACCAACTTGCCCTACTTACATTCAATCTGGATATAAAGAATCACATTCCCCGCGCGGACGTATTG
CATTAATGAAAGCGGTCGTTGACGGTTTAATTGAGCCAGATGAAGATGTTGAAAACACATTAAACGTTTGCCTTGGCTGC
CGCGCTTGTGAACCTGTTTGTCCATCTGGTGTGAATTATGGCCATTTATTAGAAGAAGCTCGTGATATTATAAATCAAAA
CAAAAAGTTCTCGATGCCAGTGAGAGCCGTTCGTAAAGTCGTTTTTGAAGGGCTATTCCCGCATCAAAATCGAATGAGAA
CATTAACCGGACTAATTGGTTTTTATCAGCGTTCTGGTTTACAAACGCTAACACATAAAACAGGGATTATGAAGTTATTC
CCTGAAACACTTGCAACAATGGATCTCGTTTTGCCTAAGGTCCCTAAAATGAAAGCAATGAAAGATCGTCCTGAATTTTT
ACATGCTGAAAGTACGAAGAAGAAACAAGTTGCATTCTTCACAGGTTGTTTAATGGATACAATGTTTTTAGAAACAAATA
ACGCAACAATGAAACTGCTTCAGTTAGCTGGTTGTGATATCGTTATTCCGAAAACACAAAGTTGCTGCGGGGCATTACAT
GGGCATGCTGGTGAGAAAAATGGGGCAAAAGAATTAGCAAAACGCAATATAAAAGCATTCGAAGATTTAAACATCGACTA
TATTATTACGAATGCTGGTGGTTGCGGAGCTTACCTCGTAGACTACGATTACCTGTTAAAAGATGATCCACAGTGGGCTG
AACGTGCAAAACAGTTTGTTTCTAAAATTAAAGATATTACTGCTATTCTAGTAGAACTAGATTTCCATAAACGTAATGAC
TTACGAGTCACGCCGCAAGTTATTACGTATCAAGACTCTTGTCACTTACGCAATGTTATGCGAACATCTTCAGAACCTCG
TATGTTACTAGAAGCAATTCAAGGCGCAACATACCGTGAAATGAAAGATGCGGATCGCTGCTGTGGTTCTGCTGGTATTT
ATAACATTGTTCATTCAGAACTATCTATGGAATTTCTAGATTACAAAATGGACCGTGTGCATGAAACAGAAGCAGCAACC
ATTGTTACCGCAAATCCAGGTTGTTTATTACAAATGAAATTAGGCATTGAACGAGAAGGTCTTTCTCATAAAATGAGAGG
AATTCACATTGTTGATTTATTATTAGAAGCAATTGAAACCAACTCGTAA

Upstream 100 bases:

>100_bases
CCGCTATGCAAGGAATTAAACACGCCTTCGATCCAAATAACATTATGAATCCAGGAAAGATGTTCGCGAAGGACACTCGT
AAAAGAGTGGTGGTTGAGCG

Downstream 100 bases:

>100_bases
CATACGTAATAATAGGAAAACGGCTATCTCCTTCGTCTATGTAAAGAGATAGCCGCTTTTTTGTCCGCTACAACATTAAA
AATATGTTACTTTATTATAA

Product: (S)-2-hydroxy-acid oxidase

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTTLNKENIQKEFKERLSENELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGC
RACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLF
PETLATMDLVLPKVPKMKAMKDRPEFLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
GHAGEKNGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFVSKIKDITAILVELDFHKRND
LRVTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAAT
IVTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS

Sequences:

>Translated_442_residues
MTTLNKENIQKEFKERLSENELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGC
RACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLF
PETLATMDLVLPKVPKMKAMKDRPEFLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
GHAGEKNGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFVSKIKDITAILVELDFHKRND
LRVTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAAT
IVTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS
>Mature_441_residues
TTLNKENIQKEFKERLSENELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVVDGLIEPDEDVENTLNVCLGCR
ACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKVVFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFP
ETLATMDLVLPKVPKMKAMKDRPEFLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALHG
HAGEKNGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFVSKIKDITAILVELDFHKRNDL
RVTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYREMKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAATI
VTANPGCLLQMKLGIEREGLSHKMRGIHIVDLLLEAIETNS

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=444, Percent_Identity=30.8558558558559, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1786497, Length=242, Percent_Identity=29.3388429752066, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: 1.1.3.15

Molecular weight: Translated: 49824; Mature: 49692

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
8.1 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLNKENIQKEFKERLSENELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVV
CCCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
DGLIEPDEDVENTLNVCLGCRACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKV
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFPETLATMDLVLPKVPKMKAM
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KDRPEFLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
HCCCHHHCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCEEECCCHHHHHHHH
GHAGEKNGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFV
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECHHHHCCCHHHHHHHHHHH
SKIKDITAILVELDFHKRNDLRVTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYRE
HHHHHHHHHHHEECCCCCCCCEECCEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCHHHH
MKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAATIVTANPGCLLQMKLGIEREG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCC
LSHKMRGIHIVDLLLEAIETNS
CHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTLNKENIQKEFKERLSENELLNCMRCGFCLPTCPTYIQSGYKESHSPRGRIALMKAVV
CCCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHH
DGLIEPDEDVENTLNVCLGCRACEPVCPSGVNYGHLLEEARDIINQNKKFSMPVRAVRKV
HHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VFEGLFPHQNRMRTLTGLIGFYQRSGLQTLTHKTGIMKLFPETLATMDLVLPKVPKMKAM
HHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KDRPEFLHAESTKKKQVAFFTGCLMDTMFLETNNATMKLLQLAGCDIVIPKTQSCCGALH
HCCCHHHCCCCCCHHHHHHHHHHHHHHHHEECCCHHHHHHHHCCCCEEECCCHHHHHHHH
GHAGEKNGAKELAKRNIKAFEDLNIDYIITNAGGCGAYLVDYDYLLKDDPQWAERAKQFV
CCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECHHHHCCCHHHHHHHHHHH
SKIKDITAILVELDFHKRNDLRVTPQVITYQDSCHLRNVMRTSSEPRMLLEAIQGATYRE
HHHHHHHHHHHEECCCCCCCCEECCEEEEECCHHHHHHHHHCCCCHHHHHHHHCCCHHHH
MKDADRCCGSAGIYNIVHSELSMEFLDYKMDRVHETEAATIVTANPGCLLQMKLGIEREG
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCC
LSHKMRGIHIVDLLLEAIETNS
CHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]