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The map label for this gene is amaA [H]

Identifier: 52143966

GI number: 52143966

Start: 1358007

End: 1359176

Strand: Direct

Name: amaA [H]

Synonym: BCZK1263

Alternate gene names: 52143966

Gene position: 1358007-1359176 (Clockwise)

Preceding gene: 52143973

Following gene: 52143964

Centisome position: 25.62

GC content: 38.72

Gene sequence:

>1170_bases
ATGAATAGAGTGTGGAAGAGTTTAATTGCGGAAGAAAATATCGTGAAATGGAGAAGACATTTTCATAAGTATCCGGAATT
ATCGTTTCATGAGAAAGAAACTTCACAATTTATATATGATACATTATGCGCATTTTCTTCTTTTGAAGTAACGAGACCGA
CGAAGTATAGTGTACTAGCAATTAAAAGAGGAACGGAACAAGGGAAAGTAGTTGCGATTCGAGCTGATATAGATGCTCTG
CCAATTCAAGAGGAGACAAGTAAATCTTATACGTCTGTGAATAAAGGAATGATGCATGCATGTGGACATGATGCTCACGC
AGCTATTTTGTTAAGTACAGCAGAGGTACTGTCAAATATAAAGGAAGATTTTGCAGGGGAGATTCGTCTATTTTTTCAGC
ACGCAGAAGAGGTATATCCCGGCGGGGGACAAGAAATGGTTGAGGCAGGTGTAATGGACGGTGTTGATTATGTAATAGGT
TTACATGTTATGTCTGGACTTGAGAGCGGGAAGATAGGAATTGCGTATGGGCCGATGATGGCGGCACCAGACGTATTTAC
AGTTGAAATTCAAGGGAAAGGGGGACATGCGGCGAGGCCGGAGGAAACGATAGATCCTATTGCTATCGGAGCGCAGATTA
TTACAAATCTACAACATATTGTATCAAGAAATACAAGTGCTTTCATGCAAAGAGTCGTTTCAGTTACGCAGTTTCATGGG
GGAATGGCTGATAATATTATTCCTAGTGCAGCAACTTTAATGGGCACTGTTCGTTCATTTAATCAAGCATTAAGGGTGGA
AGCAGAAGAGAAAATTGAAAAAATCGTGAAAGGAATTACAAAAGCACATGGAGGGGCGTATACATATACGTATCGATATG
GGTATGATCCAGTGATTAATGATGAATATATTACGAAAGTAGTAGAAGAAAGTGCAATACATTTGTTTGGGAATGAGCGT
GTTGTGAAGCTTGAACCTTCTATGGGAGGAGAAGATTTTTCAGCATATTTAAGAAAAGCACCTGGTTGTTTTATTAAACT
AGGAACTGGTAATGAAAAGATAAATACTTGCTATCCGCATCATCATCCAAAATTTGATGTAGATGAATCAGCGCTAATTA
GTGGAGTGGAATTATTTTTAGAGACAGCGATAAGACTACTAAAATCATAG

Upstream 100 bases:

>100_bases
ACGATAAGAATACGAAAAAATAGAGTATTTGTTTTTAGTAATCACTCGAGACAGAGTAAAGTTTACAATTTCTATCTCTT
TTGAAAAGGAGGAAATTATC

Downstream 100 bases:

>100_bases
AGAAACATAAAAAAAGGCAACTGCGCTCTATCGCAGTTGCTTTTATTTCTTTCCGTCAGAGAGGGCTGAGAAAGAACTAT
GCTCATTTATAGTATATGAA

Product: aminoacylase (N-acyl-L-amino acid amidohydrolase)

Products: NA

Alternate protein names: L-aminoacylase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MNRVWKSLIAEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCAFSSFEVTRPTKYSVLAIKRGTEQGKVVAIRADIDAL
PIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNIKEDFAGEIRLFFQHAEEVYPGGGQEMVEAGVMDGVDYVIG
LHVMSGLESGKIGIAYGPMMAAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
GMADNIIPSAATLMGTVRSFNQALRVEAEEKIEKIVKGITKAHGGAYTYTYRYGYDPVINDEYITKVVEESAIHLFGNER
VVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNEKINTCYPHHHPKFDVDESALISGVELFLETAIRLLKS

Sequences:

>Translated_389_residues
MNRVWKSLIAEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCAFSSFEVTRPTKYSVLAIKRGTEQGKVVAIRADIDAL
PIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNIKEDFAGEIRLFFQHAEEVYPGGGQEMVEAGVMDGVDYVIG
LHVMSGLESGKIGIAYGPMMAAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
GMADNIIPSAATLMGTVRSFNQALRVEAEEKIEKIVKGITKAHGGAYTYTYRYGYDPVINDEYITKVVEESAIHLFGNER
VVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNEKINTCYPHHHPKFDVDESALISGVELFLETAIRLLKS
>Mature_389_residues
MNRVWKSLIAEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCAFSSFEVTRPTKYSVLAIKRGTEQGKVVAIRADIDAL
PIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNIKEDFAGEIRLFFQHAEEVYPGGGQEMVEAGVMDGVDYVIG
LHVMSGLESGKIGIAYGPMMAAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
GMADNIIPSAATLMGTVRSFNQALRVEAEEKIEKIVKGITKAHGGAYTYTYRYGYDPVINDEYITKVVEESAIHLFGNER
VVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNEKINTCYPHHHPKFDVDESALISGVELFLETAIRLLKS

Specific function: Hydrolyzes most efficiently N-acetyl derivatives of aromatic amino acids but is also active on other amino acids. L- stereospecific [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=423, Percent_Identity=25.7683215130024, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.14 [H]

Molecular weight: Translated: 43035; Mature: 43035

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVWKSLIAEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCAFSSFEVTRPTKYSVLA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
IKRGTEQGKVVAIRADIDALPIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNI
EECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEDFAGEIRLFFQHAEEVYPGGGQEMVEAGVMDGVDYVIGLHVMSGLESGKIGIAYGPMM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCC
AAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
GMADNIIPSAATLMGTVRSFNQALRVEAEEKIEKIVKGITKAHGGAYTYTYRYGYDPVIN
CCHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
DEYITKVVEESAIHLFGNERVVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNEKINTCYPH
HHHHHHHHHHHHEEEECCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
HHPKFDVDESALISGVELFLETAIRLLKS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNRVWKSLIAEENIVKWRRHFHKYPELSFHEKETSQFIYDTLCAFSSFEVTRPTKYSVLA
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEE
IKRGTEQGKVVAIRADIDALPIQEETSKSYTSVNKGMMHACGHDAHAAILLSTAEVLSNI
EECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KEDFAGEIRLFFQHAEEVYPGGGQEMVEAGVMDGVDYVIGLHVMSGLESGKIGIAYGPMM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCC
AAPDVFTVEIQGKGGHAARPEETIDPIAIGAQIITNLQHIVSRNTSAFMQRVVSVTQFHG
CCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
GMADNIIPSAATLMGTVRSFNQALRVEAEEKIEKIVKGITKAHGGAYTYTYRYGYDPVIN
CCHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCC
DEYITKVVEESAIHLFGNERVVKLEPSMGGEDFSAYLRKAPGCFIKLGTGNEKINTCYPH
HHHHHHHHHHHHEEEECCCEEEEECCCCCCHHHHHHHHCCCCCEEEECCCCCCCCCCCCC
HHPKFDVDESALISGVELFLETAIRLLKS
CCCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8285691; 9023955 [H]