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The map label for this gene is nirA [H]

Identifier: 52143922

GI number: 52143922

Start: 1400291

End: 1401913

Strand: Direct

Name: nirA [H]

Synonym: BCZK1307

Alternate gene names: 52143922

Gene position: 1400291-1401913 (Clockwise)

Preceding gene: 52143923

Following gene: 52143920

Centisome position: 26.42

GC content: 36.23

Gene sequence:

>1623_bases
ATGAGTTATGAAAAAGTATGGGCTAACAATGAAAAATTAAATCAAACTGAGAAAAATAAATTAAAAAAAGATGGATTGGA
AATCTTTAATGATATTCCTTACTATGCAGAGAATGGCTTCGAATCTATTCCGAAAGAAGAGTGGGACGCATTCAAATGGG
CAGGGTTGTATTTACAAAGACCGAAAGAAGCTGGTTATTTTATGATGCGTGTAAATATTCCTTCTGGCATTATTACGAAT
GCACAGGCAGAAGTGCTTGCTTCTATCGCTGAGGATTATGGACGTGACGTAATAGATATTACGACAAGACAGGCGATTCA
GTTTCATTGGTTAGAGATTCATCAAATTCCAGATATTTTTAAAAGATTAGCAAGAGTGGGGCTATCTTCAGCTGGAGCGT
GTGGTGATATAACGCGTAATATTACAGGAAATCCACTTGCTGGCATTGATGCAAATGAATTATTTGATACAACGCATATT
GTGAAAGAGGTATATGACTATTTCCAACATAATGAAGAGTTTTCTAACTTACCACGTAAATTTAAAATGTCTATTAGTTC
TAACATTTATAACGCAGCAAACGCAGAGATTAACTGTGTTGCCTTTACACCTGCAACGAAAGAGATAGATGGTGAGAAAA
AAGTTGGTTTTCATATAAAAGTAGGCGGTGGGTTATCGGCTCGTCCATACTTAGCTGATGAATTAGATGTATTCGTTTTG
CCAGAAGAAGTGAAGGCGGTAGCGATTGCAATTGCTACTATATTTCGTGATTTTGGATATCGTGAAAAACGTCATTTAGC
TCGTTTGAAATTTCTTGTTGCAGACTGGGGAGCAGAGAAATTTAAAGAAAAAATAATAGAGTATACAGGACCTTTACAAA
GTAAAGGAGAAAGTGCAGTCAAAGGATGGAATGCAGGCTATTTTTATGGTGTTCAAGATCAAAAACAAGAGGGATTAAAA
TATGTAGGTTTTAATGTGCCGGTAGGTCGTTTACATGCAGAGGAAATGTTTGAGATTGCAAGAATTGCTAAGCAGTATGG
AAATGGACAAATTCGTACATGTAATTCGCAAAACTTCATTATTCCGAATGTTCCGCCGGAAAATGTAACTGGCTTACTAA
ATGAGCCGTTGTTTGAAGCAATATCAGCAAATCCGAAATCGTTTATTGGTCACGCGGTTTCTTGTACTGGTATTGAATAT
TGCAATCTTGCATTAGTAGAAACGAAAGAAAGATTACGAAAAATTGCCGAATACTTAGATACACAAATTGCACTCGATGT
TCCGGTTCGTATTCATATGGTAGGATGTCCGAATTCATGTGGTCAACGTCAAATTGCGGACATTGGATTACAAGGTGTGA
AGATGAAAACGAAGGAAAAAGGAATTGTTGAAGCATTTGAAATATATGTAGGTGGAACGTTGTTAGATGGTGGTGCTTAT
AATCAAAAGTTAAAAGGAAAAATAGATGGTGAGGATCTACCTGATGTACTCGCATCATTTATAAGTTATTTCAAAGAAAA
TAAATTACCGGCTGAAACGTTTTATGATTTTGTAGGCCGCGTTGGTGTAGAGACATTACAAATAGCGTTAAATAACGTGT
TAGAAGAAGTAATAGCGTCTTAG

Upstream 100 bases:

>100_bases
CGGAATTAATTGTACAAACGCACAAGTATTCTATTGAAGAATGTGCAGAACAAATCGTGAAGTACTTACAAGAGCGTAGT
TTTATATAGGGGGATAAAAA

Downstream 100 bases:

>100_bases
GAGGGGCAATATGGATTTATATCGATTTGAAGCGGTATTAGTGAGTAGCATTGTTCCAATTGTTGTTGTTGCTGAAAGTG
AAGAACAGGCATTTAAGATG

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 540; Mature: 539

Protein sequence:

>540_residues
MSYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITN
AQAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHI
VKEVYDYFQHNEEFSNLPRKFKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKIIEYTGPLQSKGESAVKGWNAGYFYGVQDQKQEGLK
YVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVTGLLNEPLFEAISANPKSFIGHAVSCTGIEY
CNLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS

Sequences:

>Translated_540_residues
MSYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITN
AQAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHI
VKEVYDYFQHNEEFSNLPRKFKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKIIEYTGPLQSKGESAVKGWNAGYFYGVQDQKQEGLK
YVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVTGLLNEPLFEAISANPKSFIGHAVSCTGIEY
CNLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS
>Mature_539_residues
SYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQRPKEAGYFMMRVNIPSGIITNA
QAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIFKRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIV
KEVYDYFQHNEEFSNLPRKFKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVLP
EEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKIIEYTGPLQSKGESAVKGWNAGYFYGVQDQKQEGLKY
VGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFIIPNVPPENVTGLLNEPLFEAISANPKSFIGHAVSCTGIEYC
NLALVETKERLRKIAEYLDTQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAYN
QKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=518, Percent_Identity=25.6756756756757, Blast_Score=119, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6322597, Length=379, Percent_Identity=26.1213720316623, Blast_Score=104, Evalue=4e-23,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 60431; Mature: 60299

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQR
CCCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHCHHHHHHHHEEC
PKEAGYFMMRVNIPSGIITNAQAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIF
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHH
KRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIVKEVYDYFQHNEEFSNLPRK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHH
FKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
EEHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKIIEYTGPLQSKGESAV
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHH
KGWNAGYFYGVQDQKQEGLKYVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFI
CCCCCCEEECCCHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
IPNVPPENVTGLLNEPLFEAISANPKSFIGHAVSCTGIEYCNLALVETKERLRKIAEYLD
ECCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCEECCHHHHCCHHHHHHHHHHHHHHHHC
TQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
CEEEEECCEEEEEEECCCCCCCHHHHHHCCCCCEECCHHCCHHHHHHHEECCEEECCCCC
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SYEKVWANNEKLNQTEKNKLKKDGLEIFNDIPYYAENGFESIPKEEWDAFKWAGLYLQR
CCCHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHCHHHHHHHHEEC
PKEAGYFMMRVNIPSGIITNAQAEVLASIAEDYGRDVIDITTRQAIQFHWLEIHQIPDIF
CCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHH
KRLARVGLSSAGACGDITRNITGNPLAGIDANELFDTTHIVKEVYDYFQHNEEFSNLPRK
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCHH
FKMSISSNIYNAANAEINCVAFTPATKEIDGEKKVGFHIKVGGGLSARPYLADELDVFVL
EEHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEE
PEEVKAVAIAIATIFRDFGYREKRHLARLKFLVADWGAEKFKEKIIEYTGPLQSKGESAV
CHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHH
KGWNAGYFYGVQDQKQEGLKYVGFNVPVGRLHAEEMFEIARIAKQYGNGQIRTCNSQNFI
CCCCCCEEECCCHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEE
IPNVPPENVTGLLNEPLFEAISANPKSFIGHAVSCTGIEYCNLALVETKERLRKIAEYLD
ECCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCEECCHHHHCCHHHHHHHHHHHHHHHHC
TQIALDVPVRIHMVGCPNSCGQRQIADIGLQGVKMKTKEKGIVEAFEIYVGGTLLDGGAY
CEEEEECCEEEEEEECCCCCCCHHHHHHCCCCCEECCHHCCHHHHHHHEECCEEECCCCC
NQKLKGKIDGEDLPDVLASFISYFKENKLPAETFYDFVGRVGVETLQIALNNVLEEVIAS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]