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The map label for this gene is hmp [H]

Identifier: 52143900

GI number: 52143900

Start: 1422184

End: 1423392

Strand: Direct

Name: hmp [H]

Synonym: BCZK1330

Alternate gene names: 52143900

Gene position: 1422184-1423392 (Clockwise)

Preceding gene: 52143899

Following gene: 52143896

Centisome position: 26.83

GC content: 33.91

Gene sequence:

>1209_bases
ATGTTAAGTGAAAAAACAATTGAAATCGTAAAGTCAACAGTACCATTATTACAGGAAAAAGGCGTTGAAATTACAACGAG
ATTTTATGAAATTTTATTTTCGGAACATCCGGAGTTATTGAATATTTTCAACCATACAAATCAGAAAAAGGGAAGACAAC
AACAAGCGTTAGCAAATGCTGTTTATGCAGCTGCAACGTACATTGATAATTTAGAAGCTATTATTCCAGTTGTAAAACAA
ATTGGTCATAAGCATAGAAGTTTAGGTATTAAAGCAGAGCATTATCCGATTGTAGGTACATGTTTATTACGTGCAATTAA
AGAGGTCGCAGGTGCACCTGATGAAGTATTAAACGCATGGGGAGAAGCGTATGGTGTCATTGCTGATGCATTCATTAGCA
TTGAAGCAGAGATGTATGAAGAAGCGGCACATAAAGAAGGTGGATGGAAAGATTTCCGTAACTTTGTGGTTGTTAAAAAA
GTGAAGGAAAGCGATGTTATTACGTCATTTTATTTAAAACCTGAAGATGGAGGGAAAGTTTCTTCATTCATTCCAGGACA
ATATGTAACGGTTCAAATAAATATCGAAGGTGAAACATATACACATAATCGTCAATATAGCTTATCAGATGCTCCTGGAA
AAGAATATTATCGTATTAGTGTCAAGAAAGAAAAAGGTGTAGATACGCCGGATGGTAAAGTATCTAATTACTTACATGAT
CATGTAAAAGAAGGTGATATGTTACCAGTAAGTGCACCAGCAGGAGATTTCGTATTAAATATGGATTCAACTTTACCAGT
TGTCTTAATTAGTGGTGGAGTAGGTATTACACCAATGATGAGTATGCTAAATACGTTAATTGAACAAGATTCAAAACGTA
ATGTATGTTTTGTTCATGCAGCGATAAATAGTAATACACATGCAATGAAAGAACACGTTGAGGCAGTAGATAATGAATAC
GAACAAGTTAAAGCATATACTTGTTATTCTGCACCAACTCAAAAAGATTTAGAAATGAAGAACTTTGATAAAGAAGGTTT
TGTTGAAAGAGAATGGTTACAAACTATTATTCCGACAACTGAAGCAGAGTTTTATTTCTGTGGTCCAGTACCATTTATGA
AACATATAAATGCTGTACTAACTGATTTAGGTGTGAAACAAGAACATATTCATTATGAATTTTTCGGTCCAGCAGCAAGC
TTACAATAA

Upstream 100 bases:

>100_bases
TTTCACAAAAACACCGTGTTAATAGTGATTAAAATCACAATCTGTATTATACAATTCAGTTAAACTAAAAACAGTTAAGG
AAATAGAGGAGTGGGATACA

Downstream 100 bases:

>100_bases
ATTGAGCTTTAAGAAGCAAAAAAAACGAGGTGTTATTTTACCTCGTTTTTTATTTGTTCTCGTAATAGTCGATTTACTGT
TTCACGGCGTATACCGATTA

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTQKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ

Sequences:

>Translated_402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTQKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ
>Mature_402_residues
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANAVYAAATYIDNLEAIIPVVKQ
IGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAWGEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKK
VKESDVITSFYLKPEDGGKVSSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHAAINSNTHAMKEHVEAVDNEY
EQVKAYTCYSAPTQKDLEMKNFDKEGFVEREWLQTIIPTTEAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAAS
LQ

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=397, Percent_Identity=45.5919395465995, Blast_Score=356, Evalue=2e-99,
Organism=Escherichia coli, GI1787658, Length=252, Percent_Identity=23.4126984126984, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1787098, Length=219, Percent_Identity=27.3972602739726, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1788104, Length=249, Percent_Identity=26.5060240963855, Blast_Score=65, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321673, Length=413, Percent_Identity=42.3728813559322, Blast_Score=275, Evalue=1e-74,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 45041; Mature: 45041

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHCCHHHHHHHHH
VYAAATYIDNLEAIIPVVKQIGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKKVKESDVITSFYLKPEDGGKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCEEEEEECCCCCCCC
SSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
CCCCCCEEEEEEEEECCCEEECCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHA
HHCCCCEEEECCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEE
AINSNTHAMKEHVEAVDNEYEQVKAYTCYSAPTQKDLEMKNFDKEGFVEREWLQTIIPTT
EECCCHHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHCCCC
EAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAASLQ
CCCEEEECCCHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC
>Mature Secondary Structure
MLSEKTIEIVKSTVPLLQEKGVEITTRFYEILFSEHPELLNIFNHTNQKKGRQQQALANA
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHCCHHHHHHHHH
VYAAATYIDNLEAIIPVVKQIGHKHRSLGIKAEHYPIVGTCLLRAIKEVAGAPDEVLNAW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHH
GEAYGVIADAFISIEAEMYEEAAHKEGGWKDFRNFVVVKKVKESDVITSFYLKPEDGGKV
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCEEEEEECCCCCCEEEEEECCCCCCCC
SSFIPGQYVTVQINIEGETYTHNRQYSLSDAPGKEYYRISVKKEKGVDTPDGKVSNYLHD
CCCCCCEEEEEEEEECCCEEECCCEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHH
HVKEGDMLPVSAPAGDFVLNMDSTLPVVLISGGVGITPMMSMLNTLIEQDSKRNVCFVHA
HHCCCCEEEECCCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCEEEEEE
AINSNTHAMKEHVEAVDNEYEQVKAYTCYSAPTQKDLEMKNFDKEGFVEREWLQTIIPTT
EECCCHHHHHHHHHHHCCHHHHHEEEEEECCCCCCCCHHHCCCCCCCHHHHHHHHHCCCC
EAEFYFCGPVPFMKHINAVLTDLGVKQEHIHYEFFGPAASLQ
CCCEEEECCCHHHHHHHHHHHHCCCCHHHEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]