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The map label for this gene is pbp2A [H]

Identifier: 52143893

GI number: 52143893

Start: 1427489

End: 1429531

Strand: Direct

Name: pbp2A [H]

Synonym: BCZK1336

Alternate gene names: 52143893

Gene position: 1427489-1429531 (Clockwise)

Preceding gene: 52143896

Following gene: 52143888

Centisome position: 26.93

GC content: 35.44

Gene sequence:

>2043_bases
ATGAAATTGAAGAACACTCACTTAAAAAAAATGCAGGAGTGGTTCAACAAGAGAAAGAAGCTTCGTAATTTATTAATTAT
ATTAGGAAGTTTACTCGCTACTCTATTTATTGCTATAAATATAATAATTTCCATTCAAGACATATCTGAATTAAAGCAAG
CTGTTCCGCAACCGACTTTAATTTATGATGCAAATAATGAAGTTGCGACTAAATTAGCTTCTTCTAAAACAGAAGGCGTA
AAAAAGAAAGATATTCCTGATATTATGGTTCAAGCAATTGTTGCTGTAGAAGATAAGGAATTTTTTAACCATCATGGTAT
TTACTATAGTGGAATTATAAGTGCGGTTTTTAAAAATATTACAGCAGGAGAAGTTGTAGCAGGGGGAAGTACAATTACAC
AACAACTTGCGAAAAATGTATTTCTGACACAAGACCGCACATACTCTCGAAAAATAAAGGAATATTTTTTAACAAAAAAA
ATAGAGCGTACGTATACGAAAGATGAAATCATTGAAATGTATATGAACCAAATTTATTTTGGTGAAGGTGCTTGGGGTAT
AAAAAGGGCAGCTAAATCTTATTTTGATAAAGATGTAAAAGATTTAACAATTTCAGAAGCTGCAACGATTGCAGGCTTGA
TTAAAGCACCATCTGCGTATTCACCTTATAAAAACTTTAACAAGTCAATTGAAAGACGTAATGTCGTATTAAGTTTAATG
AAAGAGCAAGGGTATATTTCTGACGAACAGTATAATAAAGAAAAAGAGAGCGGACTTGTGTTAAAACGTGGCGTTGATGA
TAAATACAAGGGAAAATACTCTCAATATGTAGACTATATTGTTAGAGAAGCGATGGACAAGTACGAATTAACACAAAATG
AAATTTTAGCAGGTGGTTATCGTATTTATACAGAGCTTGATCCAAAAAAACAACAGGCTGTAGAAGACGTTGTGAATAAT
GATAGTTATTTCAAAGATAGTGGCTCAGACCAACTTATGCAAACGGGTGTAGTTCTTATGAATCCAAAAACAGGTGGTGT
ACCAGCTCTAGTAGGAGGCAGAGGGCCCTATCAGTTTTTACAGTTTAACCATGCAACACAATTAAAAAGACAACCTGGCT
CAACGCTAAAGCCTTTGGCTGTATATGTACCAGCGTTAGAACAAGGATATGAAGTATACGATATATTAAAAGATGAACCA
TTTAACATTAAAGAATATAAACCACAAAATAGCGATCATACTTTTCACGGTGATGTGACAATGTATGAAGCTGTGGCAAA
ATCGTATAATGTTTCGGCTGTGTGGCTATTAGAACAAATTGGATTAGATAAAGGATTGAAATCTTTAGAGCGATTTGGTA
TTCCATTAGTACCAGAAGATCGTACGTATCCGATTGCCTTAGGCGGTATGCATGTGGGGACATCTCCATTTGTAATGGCT
CAAGCATACAGTACATTTGCAAATGACGGTGTCCAAGTGGAAGCTCATGCAATTAGAGAGATACAAAATGCTGAAGGTGA
AACGATTGGAAAGTGGTATAAGAAAGAGACACGAGTGACGAGCGAGAAAATTTCTCAAAAGATGACATATTTATTAAAGG
GTGTTGTCGAAAAGGGAACAGGCGAAAAGGCGAAAGTTACTAATGTTGATACAGCAGGTAAGACAGGAACTACTCAAATT
GTAAATGGCCCGAGCACCGGTGCAAAGGATTCATGGTTTGTTGGATACACACCAGATTTGGTAGGTGCGATTTGGGTAGG
ATATGATAAAACAGATAGCGATCATTATGTTCCAGGAGGTAGTCAAATTACGACGACAATGTTCCGGGATATTATGAAAA
AAGCGAACGCAAATCCAGCTCAAAAAGCATTCCAGTTATCACTCATATCTGAGGCTGATTATAAAAAACAATTGACTACA
ATAGAAGAGGAAAAACGAAGAAAAGAAGAAGAGAAGAGAAGAAAAGAAGAAGAACAACAACGAAAACAAGAGCAACAAGA
GTGGCTTGATAAAGTGAAAGAATGGATTCCTTCATTTTGGTAA

Upstream 100 bases:

>100_bases
TTTACACTACAGGAAAGACTACGTTATTATAATAAATTTAAAAGGAGGTATAATCTTCCTTTTGAAAAGAAGAGTGTAAA
CAAACGAAGGAAGTGAACGA

Downstream 100 bases:

>100_bases
AAGAAAGAGGCGAATAAAGAAATTCGCCTCTTTTTTGTATGAAAACAAAATGAAATTTTAATTGGTAGAGATTTTTGTCT
ATCTTAATTAGCTATTAATT

Product: penicillin-binding protein 2A

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 680; Mature: 680

Protein sequence:

>680_residues
MKLKNTHLKKMQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGV
KKKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNITAGEVVAGGSTITQQLAKNVFLTQDRTYSRKIKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIKRAAKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNN
DSYFKDSGSDQLMQTGVVLMNPKTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEP
FNIKEYKPQNSDHTFHGDVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
QAYSTFANDGVQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGTGEKAKVTNVDTAGKTGTTQI
VNGPSTGAKDSWFVGYTPDLVGAIWVGYDKTDSDHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTT
IEEEKRRKEEEKRRKEEEQQRKQEQQEWLDKVKEWIPSFW

Sequences:

>Translated_680_residues
MKLKNTHLKKMQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGV
KKKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNITAGEVVAGGSTITQQLAKNVFLTQDRTYSRKIKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIKRAAKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNN
DSYFKDSGSDQLMQTGVVLMNPKTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEP
FNIKEYKPQNSDHTFHGDVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
QAYSTFANDGVQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGTGEKAKVTNVDTAGKTGTTQI
VNGPSTGAKDSWFVGYTPDLVGAIWVGYDKTDSDHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTT
IEEEKRRKEEEKRRKEEEQQRKQEQQEWLDKVKEWIPSFW
>Mature_680_residues
MKLKNTHLKKMQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTLIYDANNEVATKLASSKTEGV
KKKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNITAGEVVAGGSTITQQLAKNVFLTQDRTYSRKIKEYFLTKK
IERTYTKDEIIEMYMNQIYFGEGAWGIKRAAKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGYRIYTELDPKKQQAVEDVVNN
DSYFKDSGSDQLMQTGVVLMNPKTGGVPALVGGRGPYQFLQFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEP
FNIKEYKPQNSDHTFHGDVTMYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
QAYSTFANDGVQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGTGEKAKVTNVDTAGKTGTTQI
VNGPSTGAKDSWFVGYTPDLVGAIWVGYDKTDSDHYVPGGSQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTT
IEEEKRRKEEEKRRKEEEQQRKQEQQEWLDKVKEWIPSFW

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=531, Percent_Identity=32.015065913371, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI87082258, Length=283, Percent_Identity=39.5759717314488, Blast_Score=198, Evalue=1e-51,
Organism=Escherichia coli, GI1788867, Length=517, Percent_Identity=27.4661508704062, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1789601, Length=143, Percent_Identity=37.0629370629371, Blast_Score=93, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 77073; Mature: 77073

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKNTHLKKMQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IYDANNEVATKLASSKTEGVKKKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNI
EEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
TAGEVVAGGSTITQQLAKNVFLTQDRTYSRKIKEYFLTKKIERTYTKDEIIEMYMNQIYF
CCCCEEECCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GEGAWGIKRAAKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGY
HHCCCCCCHHHCCHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHEEECCE
RIYTELDPKKQQAVEDVVNNDSYFKDSGSDQLMQTGVVLMNPKTGGVPALVGGRGPYQFL
EEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCEEEECCCCCCCCEEECCCCCHHHH
QFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEPFNIKEYKPQNSDHTFHGDVT
HCCHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCEEECCHH
MYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
HHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHH
QAYSTFANDGVQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGT
HHHHHHCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GEKAKVTNVDTAGKTGTTQIVNGPSTGAKDSWFVGYTPDLVGAIWVGYDKTDSDHYVPGG
CCCCEEECCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHHHHEEECCCCCCCCCCCCCC
SQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEEEKRRKEEEQQ
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKQEQQEWLDKVKEWIPSFW
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKLKNTHLKKMQEWFNKRKKLRNLLIILGSLLATLFIAINIIISIQDISELKQAVPQPTL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
IYDANNEVATKLASSKTEGVKKKDIPDIMVQAIVAVEDKEFFNHHGIYYSGIISAVFKNI
EEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCC
TAGEVVAGGSTITQQLAKNVFLTQDRTYSRKIKEYFLTKKIERTYTKDEIIEMYMNQIYF
CCCCEEECCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCC
GEGAWGIKRAAKSYFDKDVKDLTISEAATIAGLIKAPSAYSPYKNFNKSIERRNVVLSLM
CCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
KEQGYISDEQYNKEKESGLVLKRGVDDKYKGKYSQYVDYIVREAMDKYELTQNEILAGGY
HHCCCCCCHHHCCHHHCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCHHHEEECCE
RIYTELDPKKQQAVEDVVNNDSYFKDSGSDQLMQTGVVLMNPKTGGVPALVGGRGPYQFL
EEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCEEEECCCCCCCCEEECCCCCHHHH
QFNHATQLKRQPGSTLKPLAVYVPALEQGYEVYDILKDEPFNIKEYKPQNSDHTFHGDVT
HCCHHHHHHCCCCCCCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCEEECCHH
MYEAVAKSYNVSAVWLLEQIGLDKGLKSLERFGIPLVPEDRTYPIALGGMHVGTSPFVMA
HHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCCHHHH
QAYSTFANDGVQVEAHAIREIQNAEGETIGKWYKKETRVTSEKISQKMTYLLKGVVEKGT
HHHHHHCCCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GEKAKVTNVDTAGKTGTTQIVNGPSTGAKDSWFVGYTPDLVGAIWVGYDKTDSDHYVPGG
CCCCEEECCCCCCCCCCCEEECCCCCCCCCCEEECCCHHHHHHEEECCCCCCCCCCCCCC
SQITTTMFRDIMKKANANPAQKAFQLSLISEADYKKQLTTIEEEKRRKEEEKRRKEEEQQ
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKQEQQEWLDKVKEWIPSFW
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]