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The map label for this gene is dacB [H]

Identifier: 52143878

GI number: 52143878

Start: 1445708

End: 1446832

Strand: Direct

Name: dacB [H]

Synonym: BCZK1351

Alternate gene names: 52143878

Gene position: 1445708-1446832 (Clockwise)

Preceding gene: 52143879

Following gene: 52143875

Centisome position: 27.27

GC content: 36.09

Gene sequence:

>1125_bases
ATGAGACGAATTTGTATAATCATAACGCTTCTTATTATTTATGCAAGCGTTATGCCAATTCCTACATATGCAAAGATGAA
CAGCAATGTCAGTGCTCGTAATGCTGTATTAATGGAACAACATTCTGGACGTGTATTATACGGAAAAGCAGAACATGAGC
CACAAAAGATTGCTAGTATAACAAAAATTATGACAGCCTTATTAGCTGCTGAATCAGGAAAGATGAAAGAAAGGGTATTA
GTAAGTAATGAAGCGGTCAGGGTCGAAGGATCTGCAATTTATTTAAAGCCTGGACAAAAAGTGAAGCTAGAGGATTTAGT
ATACGGACTTATGCTTAGATCTGGTAATGACGCAGCACAAGTAATTGCTGAAAATGTAGGAGGGAGTATAGAAGGGTTCG
TATATTTAATGAATGAAAAGGCGAAACAGATTGGAATGAAAGATACTCACTTTTCAAACCCACATGGTTTGGATGGAGAT
GGATCTCATTATTCGTCGGCCTATGATATGGCACTTTTAACGAGATATGCAATGGGGAACGAGACTTTTAAGAAAATTTT
TGGAACAAAAACGTATAAATCAGATTCGTGGGATTATCCGTGGAAAAACAAACATAAACTTGTGACGTCGTATTATGAAT
TTGCAACAGGGGGAAAAACAGGTTTTACGAAGAAAGCAGGAAGAACGCTTGTTACAACAGCATCAAAAGATGGACTAGAT
CTAATTGTCGTAACTTTGAGCGCTTCTAGTGATTGGGATGATCATATGAATTTATTTGATAAAGGTTTTGACCGTTTCGA
ACAAACAAAAGTTTTAGGACAAGGAGCACTTGCTGAAATAACCGAAAAGAAATACGCCAATCATGTTTACACGAAAAATA
GTTTTTCAGTACCTTTAACTGAAGAGGAAAAAAAGAGCGTAGTATTAAAAGTTGAACTAGATAAAAGTGCAAAGCTTGTG
GATGGAGTAAAGGTTGGAAAGACAGACGTTTATGTTGGCAATGAGAAAGTTGGAGAACGGAATTTATTTTATAGTAAACG
AAAGTTAGTAGCTACAACGGGAATGTACTGGAATAATGTGAAAGAAATTTTTTCTTACATGATAGGTGTTGGGAACGATG
GTTAA

Upstream 100 bases:

>100_bases
CTGCGTCTTTTTTATTGGAAGGAGAAGGAGATTTCGTTCATATTTTAAAAGGCCAAGCATACACTTGTACAAAAAGTGCC
AAAAGGACGTGGGGGGAAAT

Downstream 100 bases:

>100_bases
TCTCGTATGGGTAGCGATGGCTGTCATTGGGATTGTGTATGCGATGATAAATGGAACGATGGAAGAAATAAATAAAGCTG
TATTTGATGGAGCAAAAGAT

Product: D-alanyl-D-alanine carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MRRICIIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKERVL
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGNDG

Sequences:

>Translated_374_residues
MRRICIIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKERVL
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGNDG
>Mature_374_residues
MRRICIIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKERVL
VSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGD
GSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAKLV
DGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNVKEIFSYMIGVGNDG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=372, Percent_Identity=29.8387096774194, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1787062, Length=266, Percent_Identity=36.4661654135338, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI1786851, Length=262, Percent_Identity=33.206106870229, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87082054, Length=211, Percent_Identity=26.0663507109005, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41566; Mature: 41566

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRICIIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASI
CCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHEEEEECCCCEEEEECCCCCHHHHHHH
TKIMTALLAAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQ
HHHHHHHHHHCCCCCHHEEEECCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHH
VIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGDGSHYSSAYDMALLTRYAMGN
HHHHHCCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCCE
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLT
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCCCC
EEEKKSVVLKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNV
CCCCCEEEEEEEECCCCHHHCCEEECCEEEEECCCCCCCCCHHHHCCEEEEECCHHHHHH
KEIFSYMIGVGNDG
HHHHHHHHCCCCCC
>Mature Secondary Structure
MRRICIIITLLIIYASVMPIPTYAKMNSNVSARNAVLMEQHSGRVLYGKAEHEPQKIASI
CCHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHEEEEECCCCEEEEECCCCCHHHHHHH
TKIMTALLAAESGKMKERVLVSNEAVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQ
HHHHHHHHHHCCCCCHHEEEECCCEEEEECCEEEECCCCEEEHHHHHHHHHHCCCCHHHH
VIAENVGGSIEGFVYLMNEKAKQIGMKDTHFSNPHGLDGDGSHYSSAYDMALLTRYAMGN
HHHHHCCCCCEEEEEEEECHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
ETFKKIFGTKTYKSDSWDYPWKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLD
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHCCCEEEEECCCCCCE
LIVVTLSASSDWDDHMNLFDKGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLT
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEECCCCCCCCC
EEEKKSVVLKVELDKSAKLVDGVKVGKTDVYVGNEKVGERNLFYSKRKLVATTGMYWNNV
CCCCCEEEEEEEECCCCHHHCCEEECCEEEEECCCCCCCCCHHHHCCEEEEECCHHHHHH
KEIFSYMIGVGNDG
HHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]