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The map label for this gene is ponA [H]

Identifier: 52143800

GI number: 52143800

Start: 1515899

End: 1518592

Strand: Reverse

Name: ponA [H]

Synonym: BCZK1431

Alternate gene names: 52143800

Gene position: 1518592-1515899 (Counterclockwise)

Preceding gene: 52143799

Following gene: 52143810

Centisome position: 28.65

GC content: 37.97

Gene sequence:

>2694_bases
ATGTCAGATAATTATCGTTCTCGTACAGAACGAAATCATGTAAAAAATCAAAAGCAAGAAACAAATAAAGAAAAAAAACC
AAAGAAAAAAGGCTCCTTTTTTAAAAAATTCCTTGTAGGTTGTCTACTTCTTGGTATCGTTGGTCTTGTAGCTGGCGTTT
CTGCTTTCTTTGTTATGGTGAAGGACGCTCCAAAATTAGACAAATCAAAACTTGTCAATCCTTTATCAACAAAGTTTCTT
GATAAAGACGGGAACTTTTTCTATGAATACGGTGCTGAAAAACGAACCCACGTTACGTATGATCAAATTCCAAAAGTAGT
TGAAAATGCATTCCTTGCAACTGAGGACTCACGTTTCTACGATCATAATGGAATTGATTTTAAACGAACTACAAAAGCAG
TTATGGAAAATGTCACTGGAGGTTTCGGATCTCAAGGTGGGAGTACGATTACACAACAAGTAGTCAAAAACTATTTTCTA
ACGATGGACAAAACTGCAAAGAGAAAAGTACAAGAATGGTACTTATCTTATAAATTAGAGCAACAATACTCTAAACATGA
GATTTTAGAAATGTACTTAAATAAGATTAATTTAGGTAACCGTTCATATGGTATTGCGACAGCAGCGCAAAAATATTATG
GTAAAGATTTAAAAGACTTGCAATTACATGAAGCTGCTATGCTCGCTGGTTTACCCCAAGGTCCAAACATTTACGATCCA
ACGAAAAAGGAAAATGTTGAACGAGCAACAAATCGTCGTAATGTCGTATTGTCATTAATGAATCGACATGGTTATATAAC
AAAAGAAGAAATGAATAACGCAGTTAAGATCCCAGTAACAGATGGTCTTCAACCGTCTTCAGAAATAACTGAGATGAAGT
ATCAAGCATTTCTAGATGCGGTTGTAAAAGAAGTTGAAAAAGAATATCCTGATGTAAATATTGGTTCAGATGGTTTAACG
ATTCATACAACACTTGATCAAGATGCTCAAGATTATGCTGAAAAAATTATGGATGGCAATCTTATTAAGTATCCAAACGA
TCAATTCCAAGGATCATTCGTATTTATGGATACACAATCTGGTGAAGTTCGAGCAATTGGAGCTGGGCGTAAAGGAAGTA
AGTCTACTTTCAAAGGTCATAATATGGCCACCGATTTAAAACGCCAAGTCGGTTCAACAATGAAACCAATTTTTGACTAC
GGCCCAGCAATTGAAAATTTACAATGGTCTACATACCATCAATTAAATGACTCAGAGTATACGTATTCCAATGGTAAAAA
GATACGAAATGCAACAAATAGTTACAAAGGTGACGTTTCACTACGTGAAGCTTTGAAAAAGTCATTAAACATCCCAGCTT
TAAAAACAGCCCAAGCGGTTGGTCTTCCTAAGTCACAAGCGTTTGCTGAAGGTTTAGGTATGACATTTAAAGATGGCAAA
ACATTTGAATCAACAGCAATTGGTAGTAACGATAGTTCTCCATTACAATTAGCAGGTGCATATGCAGCCTTTGGTAATGG
TGGGAACTACAATAAACCGCACTTCGTAAAAGAAGTTACCTTCCCAGATGGGAAAAAGAAAAGTTTTAAACCGAAAGAAC
AACGTGCGATGCAAGACTACACAGCTTACATGGTGACTGACGTTCTTCGTGACGTAGTAAAACCTGGTTCTGGCGGTACT
GGTCCAACAGCATACGTTTCAGGTGTTGATGTTGCCGGTAAAACAGGAACACAAAACTTTGATGCAGACGTTATAAAAGA
ATATGGTATTCCAGCTGATGCAAACAGAGATAGCTGGTTCGCTGGATATACACCGCAATATACAATGGCAGTATGGACTG
GTTACGAAAAAAATGGCCCAGAAAACTACATTAGTGATCGTTATACTAGAATTGCACAGCAAATGTTCCAAGTAATGATG
AGCAAATTCGCTACAGATAAATCCCGTTTCGAACGTCCTTCTTCTGTACAAGAAATAAACGGAGAGTTATATGTAAAAGG
TGCGAAGAAAGATGCAATTAAACAAATTAAAGTAGATGCACCTAGTGGTCTTAATGTCACTTTCGATGGCGCTAGCACAG
TTACACTAAACTGGTCTGGACCAGCAGAAGTTGATGCGTATGCAGCAAGCTATAAAGCGACTGACGGTTCAAGTGGTAGT
TTATCAGTAAAAGGTACAACAGCTACTCTTGGTGGTATTAAACCAGGCGTTACTTACAGCTTCTCTGTAGTAGCGAAAAA
AGGTACTGGAACAAGCCCTGCAGTTGGGGCATCCTTCACTGCGCCTGGCGGAACTCCAGACGCGAAGAAAGCCGAGGAAG
AAGCTAAGAAGAAGGCTGAAGAAGAAGCTAAGAAAAAAGCTGATGAGGAAACTCAGAAAAAGGCCAATGAAGATAAACTA
AAACAGGAAGAAGCTACTAAAAAAGCTGAAGAAGAAGCTAAGAAACAACAAGAACAACAACGTAAACAGCAAGAAGAAGC
TCAAAAGAAAGCTGAAGAGGAAGCTAGAAAAAAAGCTGAAGAAGAAGCTAAGAAACAGCAAGAAGAGGCACAAAAGAAAG
CTGAAGAGGAAGCTAGAAAAAAAGCTGAAGAAGAAGCTAAAAAACAACAAGAGCAACAGCAGCAAGAACAACAACAAAAT
ACCGGAGGAGATACACCTCACGCAGACGGAAATGTTGTTACAACAGAGTCTTAA

Upstream 100 bases:

>100_bases
ATATCCGTGTACTAGACACGGTTTATTTTTCGTGATAGAGTCATCACTAAGGCTCTTTTTTCGACTTTTGGGGAGAGAAT
GAAAGGTAGGAGAAAGTATA

Downstream 100 bases:

>100_bases
TACAAAAAAAGAAGTCCTTTCTCTTAACGAGAACAGGACTTCTTTTTTGTTATCATTTTCTTTACATATAGCTTTTCTAT
TTCAATATACAACTGCGCTA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP1; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 897; Mature: 896

Protein sequence:

>897_residues
MSDNYRSRTERNHVKNQKQETNKEKKPKKKGSFFKKFLVGCLLLGIVGLVAGVSAFFVMVKDAPKLDKSKLVNPLSTKFL
DKDGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFL
TMDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTDGLQPSSEITEMKYQAFLDAVVKEVEKEYPDVNIGSDGLT
IHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKGSKSTFKGHNMATDLKRQVGSTMKPIFDY
GPAIENLQWSTYHQLNDSEYTYSNGKKIRNATNSYKGDVSLREALKKSLNIPALKTAQAVGLPKSQAFAEGLGMTFKDGK
TFESTAIGSNDSSPLQLAGAYAAFGNGGNYNKPHFVKEVTFPDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGT
GPTAYVSGVDVAGKTGTQNFDADVIKEYGIPADANRDSWFAGYTPQYTMAVWTGYEKNGPENYISDRYTRIAQQMFQVMM
SKFATDKSRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGS
LSVKGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKAEEEAKKKADEETQKKANEDKL
KQEEATKKAEEEAKKQQEQQRKQQEEAQKKAEEEARKKAEEEAKKQQEEAQKKAEEEARKKAEEEAKKQQEQQQQEQQQN
TGGDTPHADGNVVTTES

Sequences:

>Translated_897_residues
MSDNYRSRTERNHVKNQKQETNKEKKPKKKGSFFKKFLVGCLLLGIVGLVAGVSAFFVMVKDAPKLDKSKLVNPLSTKFL
DKDGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFL
TMDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTDGLQPSSEITEMKYQAFLDAVVKEVEKEYPDVNIGSDGLT
IHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKGSKSTFKGHNMATDLKRQVGSTMKPIFDY
GPAIENLQWSTYHQLNDSEYTYSNGKKIRNATNSYKGDVSLREALKKSLNIPALKTAQAVGLPKSQAFAEGLGMTFKDGK
TFESTAIGSNDSSPLQLAGAYAAFGNGGNYNKPHFVKEVTFPDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGT
GPTAYVSGVDVAGKTGTQNFDADVIKEYGIPADANRDSWFAGYTPQYTMAVWTGYEKNGPENYISDRYTRIAQQMFQVMM
SKFATDKSRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGS
LSVKGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKAEEEAKKKADEETQKKANEDKL
KQEEATKKAEEEAKKQQEQQRKQQEEAQKKAEEEARKKAEEEAKKQQEEAQKKAEEEARKKAEEEAKKQQEQQQQEQQQN
TGGDTPHADGNVVTTES
>Mature_896_residues
SDNYRSRTERNHVKNQKQETNKEKKPKKKGSFFKKFLVGCLLLGIVGLVAGVSAFFVMVKDAPKLDKSKLVNPLSTKFLD
KDGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFYDHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFLT
MDKTAKRKVQEWYLSYKLEQQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDPT
KKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTDGLQPSSEITEMKYQAFLDAVVKEVEKEYPDVNIGSDGLTI
HTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQSGEVRAIGAGRKGSKSTFKGHNMATDLKRQVGSTMKPIFDYG
PAIENLQWSTYHQLNDSEYTYSNGKKIRNATNSYKGDVSLREALKKSLNIPALKTAQAVGLPKSQAFAEGLGMTFKDGKT
FESTAIGSNDSSPLQLAGAYAAFGNGGNYNKPHFVKEVTFPDGKKKSFKPKEQRAMQDYTAYMVTDVLRDVVKPGSGGTG
PTAYVSGVDVAGKTGTQNFDADVIKEYGIPADANRDSWFAGYTPQYTMAVWTGYEKNGPENYISDRYTRIAQQMFQVMMS
KFATDKSRFERPSSVQEINGELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGSL
SVKGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKAEEEAKKKADEETQKKANEDKLK
QEEATKKAEEEAKKQQEQQRKQQEEAQKKAEEEARKKAEEEAKKQQEEAQKKAEEEARKKAEEEAKKQQEQQQQEQQQNT
GGDTPHADGNVVTTES

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein. Note=Probably found all over the whole cell at low concentrations. Also localizes to the division site in vegetative cells [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 fibronectin type-III domain [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=548, Percent_Identity=31.021897810219, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI87082258, Length=318, Percent_Identity=38.0503144654088, Blast_Score=209, Evalue=8e-55,
Organism=Escherichia coli, GI1788867, Length=606, Percent_Identity=27.2277227722772, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1789601, Length=204, Percent_Identity=32.843137254902, Blast_Score=90, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR008957
- InterPro:   IPR003961
- InterPro:   IPR001264
- InterPro:   IPR013783
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00041 fn3; PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 99459; Mature: 99328

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: PS50853 FN3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
>Mature Secondary Structure 
SDNYRSRTERNHVKNQKQETNKEKKPKKKGSFFKKFLVGCLLLGIVGLVAGVSAFFVMV
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
KDAPKLDKSKLVNPLSTKFLDKDGNFFYEYGAEKRTHVTYDQIPKVVENAFLATEDSRFY
CCCCCCCHHHCCCHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
DHNGIDFKRTTKAVMENVTGGFGSQGGSTITQQVVKNYFLTMDKTAKRKVQEWYLSYKLE
CCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
QQYSKHEILEMYLNKINLGNRSYGIATAAQKYYGKDLKDLQLHEAAMLAGLPQGPNIYDP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCC
TKKENVERATNRRNVVLSLMNRHGYITKEEMNNAVKIPVTDGLQPSSEITEMKYQAFLDA
CHHHHHHHHHHHHHHHHHHHHHCCCEEHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
VVKEVEKEYPDVNIGSDGLTIHTTLDQDAQDYAEKIMDGNLIKYPNDQFQGSFVFMDTQS
HHHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCEEECCCCCCCCCEEEEECCC
GEVRAIGAGRKGSKSTFKGHNMATDLKRQVGSTMKPIFDYGPAIENLQWSTYHQLNDSEY
CCEEEEECCCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHCCCCCCE
TYSNGKKIRNATNSYKGDVSLREALKKSLNIPALKTAQAVGLPKSQAFAEGLGMTFKDGK
EECCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHCCCEECCCC
TFESTAIGSNDSSPLQLAGAYAAFGNGGNYNKPHFVKEVTFPDGKKKSFKPKEQRAMQDY
CCHHHCCCCCCCCCCEEHHHHHEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHH
TAYMVTDVLRDVVKPGSGGTGPTAYVSGVDVAGKTGTQNFDADVIKEYGIPADANRDSWF
HHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEE
AGYTPQYTMAVWTGYEKNGPENYISDRYTRIAQQMFQVMMSKFATDKSRFERPSSVQEIN
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHCC
GELYVKGAKKDAIKQIKVDAPSGLNVTFDGASTVTLNWSGPAEVDAYAASYKATDGSSGS
CEEEEECCCHHHHHHEEECCCCCCEEEECCCEEEEEECCCCCCHHHHHHHCCCCCCCCCE
LSVKGTTATLGGIKPGVTYSFSVVAKKGTGTSPAVGASFTAPGGTPDAKKAEEEAKKKAE
EEEECCEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
EEAKKKADEETQKKANEDKLKQEEATKKAEEEAKKQQEQQRKQQEEAQKKAEEEARKKAE
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAKKQQEEAQKKAEEEARKKAEEEAKKQQEQQQQEQQQNTGGDTPHADGNVVTTES
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7814321; 8760912; 9384377; 9721295; 10322023 [H]