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The map label for this gene is yjfC [C]

Identifier: 52143771

GI number: 52143771

Start: 1543389

End: 1544627

Strand: Direct

Name: yjfC [C]

Synonym: BCZK1458

Alternate gene names: 52143771

Gene position: 1543389-1544627 (Clockwise)

Preceding gene: 52143774

Following gene: 52143772

Centisome position: 29.12

GC content: 35.19

Gene sequence:

>1239_bases
ATGTCGCAGTACATAAGGGATCGAAAAGAGTTTTATTCAAAATATCCGGATTTCTGGTCGGATTTATATGAATGTGAGTA
TAGTTTATTTCATGTTTTCTCTATAACAAATGAGACTATGAAACAATTACAGTTAGCAACTGAACGAATGGGAAAAATAT
TTTTTAAGACTGCTAGACTTCTGCGTAATTTAAGTGACGAACAGCTTCTTGAACTCGGATTTCCTCCTGCGAGCTTATCG
TTCATTAGAATGAAAGGATTATATCCTGAATCTGTTATTTCGCGGTTTGATTTCGTTTTAACCGATGATAACCAAATTAA
AATGCTCGAATTTAATAGTGATACGCCAACATTTATTATGGAATGCTTTCAAATGAATGGAAAAGTTTGCGAGAAACTAG
GCTATGATAATCCAAATTCGGATCAAGAACGACTACTATCTTCTGGTATTACAAAAGCCGTTATGGAAGCGACAAAGGGA
ATAGACAACCCGAATGTTATTTTTACAGCACATCATGAACATATTGAGGATTGGAATACGACTATGTATTTGAGTCAGCT
ATGCCATGTTGAAAATAAAGTAGTGCCAATGTCAGAACTTAGAATTACAAAAGATGCTTTAGTGGATAGGGATGGAGTGC
AAATTGATGTTTTATATCGCCAAACATATCCGATAGAAGATTTAATTGAGGATCAAGATCCTGAAACAGGAGATTTGGTA
GGTGTGGAGTTATTGCAACTTGTAAAAGCCGGAAAGCTTTTTATCATCAATCCTTTGTCAGCATTTTTACTTCAGCCAAA
ATCAGTTCAGTGTCTTATTTGGGGGTTAGCAGAAGAAGAGGCCTTTTATACAAATGAGGAAAGACAGTGGATTAAAGAAT
ATATGCTACCTACATATTTAGAACCAGATTTATTTTTAGGAAAAGGTTCTTTCGTTCAAAAACCTTCATTTGGTAGAGAA
GGAGATACAATTACGATTCGCGATAAGGACGAAAATGTTTTACTTCAAAATACACACCAAACGTATAAAGCGTCGCTGCC
GATATTTCAAAAGTATACAGAACTTCCAGTCGTATCGTTGGAAACAGAAAAAGGGATGGAGAAATTGTCGTATGTGTTTG
GTTCATTTTTAATTGCTGGAAAAGCGAGTAGTATTGGTATTCGTGCTGGTGAAAAAATTACGGGGAATGAATCGTACTAT
TTACCGGTAGGTATAAAAAAGGAGGAAGTATATGATTAA

Upstream 100 bases:

>100_bases
CACTTTTAAAAATTCATCGGCGTTTAAATCGTATCAATCGTCTGCGGAATTTAAAGGCGGAATTGGAAGCGGTTCAAAGG
GAGGATTTGGGGGCTAAAAT

Downstream 100 bases:

>100_bases
TTTTTTATTATATTTAGCAGTAACACTTGGTCTTTTATGCATTGGTCTTTTCCTAATGGAAGTGACAACGAAAGTAAAAG
AATTTAAGTTAATGGCCCAA

Product: glutathionylspermidine synthase

Products: N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine; ADP; phosphate

Alternate protein names: Glutathionylspermidine Synthetase; Glutathionylspermidine Synthase-Like Protein; Glutathionylspermidine Synthase Family Protein; Glutathionylspermidine Synthase-Like

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MSQYIRDRKEFYSKYPDFWSDLYECEYSLFHVFSITNETMKQLQLATERMGKIFFKTARLLRNLSDEQLLELGFPPASLS
FIRMKGLYPESVISRFDFVLTDDNQIKMLEFNSDTPTFIMECFQMNGKVCEKLGYDNPNSDQERLLSSGITKAVMEATKG
IDNPNVIFTAHHEHIEDWNTTMYLSQLCHVENKVVPMSELRITKDALVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLV
GVELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEAFYTNEERQWIKEYMLPTYLEPDLFLGKGSFVQKPSFGRE
GDTITIRDKDENVLLQNTHQTYKASLPIFQKYTELPVVSLETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYY
LPVGIKKEEVYD

Sequences:

>Translated_412_residues
MSQYIRDRKEFYSKYPDFWSDLYECEYSLFHVFSITNETMKQLQLATERMGKIFFKTARLLRNLSDEQLLELGFPPASLS
FIRMKGLYPESVISRFDFVLTDDNQIKMLEFNSDTPTFIMECFQMNGKVCEKLGYDNPNSDQERLLSSGITKAVMEATKG
IDNPNVIFTAHHEHIEDWNTTMYLSQLCHVENKVVPMSELRITKDALVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLV
GVELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEAFYTNEERQWIKEYMLPTYLEPDLFLGKGSFVQKPSFGRE
GDTITIRDKDENVLLQNTHQTYKASLPIFQKYTELPVVSLETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYY
LPVGIKKEEVYD
>Mature_411_residues
SQYIRDRKEFYSKYPDFWSDLYECEYSLFHVFSITNETMKQLQLATERMGKIFFKTARLLRNLSDEQLLELGFPPASLSF
IRMKGLYPESVISRFDFVLTDDNQIKMLEFNSDTPTFIMECFQMNGKVCEKLGYDNPNSDQERLLSSGITKAVMEATKGI
DNPNVIFTAHHEHIEDWNTTMYLSQLCHVENKVVPMSELRITKDALVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLVG
VELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEAFYTNEERQWIKEYMLPTYLEPDLFLGKGSFVQKPSFGREG
DTITIRDKDENVLLQNTHQTYKASLPIFQKYTELPVVSLETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYYL
PVGIKKEEVYD

Specific function: Unknown

COG id: COG0754

COG function: function code E; Glutathionylspermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.1.8

Molecular weight: Translated: 47469; Mature: 47338

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQYIRDRKEFYSKYPDFWSDLYECEYSLFHVFSITNETMKQLQLATERMGKIFFKTARL
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNLSDEQLLELGFPPASLSFIRMKGLYPESVISRFDFVLTDDNQIKMLEFNSDTPTFIM
HHCCCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCEEEECCCCEEEEEECCCCHHHHH
ECFQMNGKVCEKLGYDNPNSDQERLLSSGITKAVMEATKGIDNPNVIFTAHHEHIEDWNT
HHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCHH
TMYLSQLCHVENKVVPMSELRITKDALVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLV
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCHH
GVELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEAFYTNEERQWIKEYMLPTYL
HHHHHHHHHCCCEEEECCHHHHHCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCCCCCC
EPDLFLGKGSFVQKPSFGREGDTITIRDKDENVLLQNTHQTYKASLPIFQKYTELPVVSL
CCCCEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHCCCHHHHHHCCCEEEE
ETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYYLPVGIKKEEVYD
CHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEECCCCEEEECCCCHHHCCC
>Mature Secondary Structure 
SQYIRDRKEFYSKYPDFWSDLYECEYSLFHVFSITNETMKQLQLATERMGKIFFKTARL
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRNLSDEQLLELGFPPASLSFIRMKGLYPESVISRFDFVLTDDNQIKMLEFNSDTPTFIM
HHCCCHHHHHHHCCCCCCCCCEEECCCCHHHHHHHCCEEEECCCCEEEEEECCCCHHHHH
ECFQMNGKVCEKLGYDNPNSDQERLLSSGITKAVMEATKGIDNPNVIFTAHHEHIEDWNT
HHHHCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHCHH
TMYLSQLCHVENKVVPMSELRITKDALVDRDGVQIDVLYRQTYPIEDLIEDQDPETGDLV
HHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHCCCCCCCCCHH
GVELLQLVKAGKLFIINPLSAFLLQPKSVQCLIWGLAEEEAFYTNEERQWIKEYMLPTYL
HHHHHHHHHCCCEEEECCHHHHHCCCCCCEEEEEECCCCCHHHCCHHHHHHHHHCCCCCC
EPDLFLGKGSFVQKPSFGREGDTITIRDKDENVLLQNTHQTYKASLPIFQKYTELPVVSL
CCCCEECCCCCCCCCCCCCCCCEEEEEECCCCEEEECCCHHHHHCCCHHHHHHCCCEEEE
ETEKGMEKLSYVFGSFLIAGKASSIGIRAGEKITGNESYYLPVGIKKEEVYD
CHHHHHHHHHHHHHHHHHCCCCCCCEECCCCEEECCCCEEEECCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: gamma-L-glutamyl-L-cysteinyl-glycine; spermidine; ATP

Specific reaction: gamma-L-glutamyl-L-cysteinyl-glycine + spermidine + ATP = N1-(gamma-L-glutamyl-L-cysteinyl-glycyl)-spermidine + ADP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA