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The map label for this gene is chrR [H]

Identifier: 52143743

GI number: 52143743

Start: 1584035

End: 1584769

Strand: Direct

Name: chrR [H]

Synonym: BCZK1491

Alternate gene names: 52143743

Gene position: 1584035-1584769 (Clockwise)

Preceding gene: 52143744

Following gene: 52143742

Centisome position: 29.88

GC content: 36.6

Gene sequence:

>735_bases
ATGAATGAAATGATACATAAAATGGAGCAACACGTTTCAGTTCGTAAATATAAAGAAGAGTCCATTCCGAAAGATGTAGT
AGAAAGAATGGTGCAAGCTGGGCAGCATGCTGCTTCCTCTCATTTTGTACAGGCCTATTCTGTTATATATGTAACCGATC
AAGACTTAAAGGCTAAACTAGCAGAGTTGTCTGGAAATCGACATGTGAAAGATTGCGCAGCATTCTTTGTTTGTTGTGCA
GATTTAAAACGACTTGAAATGGCATGTGAAAAACATGGTACAGAGATAAAGCATGAAGGAGTAGAAGACTTTATCGTAGC
AACGGTAGATGCTTCACTTTTTGCGCAAAACTTAGCGTTAGCAGCAGAATCGTTAGGATACGGTATTTGTTATATTGGTG
GTATTCGTAATAATCCGAGAGAAGTGAGTGAATTGCTTCATTTACCGGATAAAGTATATCCTGTATTCGGAATGACAGTC
GGTGTACCAGATGAAGATCATGGAGTAAAACCACGCTTACCAGTAGCAGCAATCCTTCATGAAAATGGATATGATGAAAA
GAAATATGATGAATTATTAAATGAATACGATGAAACGATGAGCGCGTATTATAAAGGAAGATCATCAAACAAGAAAAGCG
TCACATGGACGGAATCAATGAGTTCGTTTATGTCTAAAGAAAAAAGAATGCACATGAAAGAGTTTTTAAATGAGAAAGGA
TTAAATAAGAAATGA

Upstream 100 bases:

>100_bases
GAGCAATTCAAAAGAATTGCTCTTTTTGTGCTTATAAGTTGTGAATATTGGAAAAGATATGTTAATTTCTCATTAGGAAG
AGAAAAAAGGAGTGATTTTC

Downstream 100 bases:

>100_bases
GTAAAGAGGCAAACCTGTGATGTAGGTTTGCCTTTTTTGGAGGAGAAAGGTGATTGTCTTCGAATAAGTATAAGGGGAGG
GGATAATATGAAAAAAGTTT

Product: oxygen-insensitive NADPH nitroreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKLAELSGNRHVKDCAAFFVCCA
DLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLALAAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTV
GVPDEDHGVKPRLPVAAILHENGYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNEKG
LNKK

Sequences:

>Translated_244_residues
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKLAELSGNRHVKDCAAFFVCCA
DLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLALAAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTV
GVPDEDHGVKPRLPVAAILHENGYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNEKG
LNKK
>Mature_244_residues
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKLAELSGNRHVKDCAAFFVCCA
DLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLALAAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTV
GVPDEDHGVKPRLPVAAILHENGYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNEKG
LNKK

Specific function: Reduction Of Nitroaromatic Compounds Using NADH. Reduces Nitrofurazone By A Ping-Pong Bi-Bi Mechanism Possibly To Generate A Two-Electron Transfer Product. Major Component Of The Oxygen- Insensitive Nitroreductase Activity In E.Coli. [C]

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the flavin oxidoreductase frp family [H]

Homologues:

Organism=Escherichia coli, GI1787075, Length=242, Percent_Identity=35.9504132231405, Blast_Score=152, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016446
- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27657; Mature: 27657

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHEEEEEEECHHHHHHH
AELSGNRHVKDCAAFFVCCADLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLAL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
AAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTVGVPDEDHGVKPRLPVAAILH
HHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHCEEECCCCCCCCCCCCCCHHHHHH
ENGYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNEKG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
LNKK
CCCC
>Mature Secondary Structure
MNEMIHKMEQHVSVRKYKEESIPKDVVERMVQAGQHAASSHFVQAYSVIYVTDQDLKAKL
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHEEEEEEECHHHHHHH
AELSGNRHVKDCAAFFVCCADLKRLEMACEKHGTEIKHEGVEDFIVATVDASLFAQNLAL
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHH
AAESLGYGICYIGGIRNNPREVSELLHLPDKVYPVFGMTVGVPDEDHGVKPRLPVAAILH
HHHHHCCCEEEECCCCCCHHHHHHHHHCCHHHHHHHCEEECCCCCCCCCCCCCCHHHHHH
ENGYDEKKYDELLNEYDETMSAYYKGRSSNKKSVTWTESMSSFMSKEKRMHMKEFLNEKG
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
LNKK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA