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The map label for this gene is alaXM [H]

Identifier: 52143685

GI number: 52143685

Start: 1634249

End: 1634959

Strand: Direct

Name: alaXM [H]

Synonym: BCZK1549

Alternate gene names: 52143685

Gene position: 1634249-1634959 (Clockwise)

Preceding gene: 52143687

Following gene: 52143684

Centisome position: 30.83

GC content: 35.86

Gene sequence:

>711_bases
ATGACAACGGCGTTATATTTAGAAGATGCATACAAAACGAGTTGCGAAACAGAAGTGATAAAAGTAGAAGGGAATAAAGT
ATTTGTAAAAGAAACAGTTTTTTATCCAACTGGTGGGGGGCAGGAATGTGATACGGGTTTCATTGTACAAGACGGGTCTG
TATTTGAAGTTGAAAAGGTAAAGAAGGAGCAGGGAGAAATAGTTCACTATATAAAAGATGGAGCTCAAGTAAAATTAGGT
CCCGTTAAATTAGAGATTAATTGGGAAAGACGTCATAATTTAATGCGTCATCACTCTTTACTGCATCTTATCGGAGCTGT
CGTTTATGAAAAGTATGGAGCTCTCTGCACTGGAAATCAAATTTATCCTGACAAAGCACGTATCGATTTTAATGAGTTAC
AAGAGTTATCGAGCGTGGAAGTAGAAGGAATTGTTAAGGAGGTGAATAAACTTATTGAACAAAATAAAGAAATTTCTACT
CGTTATATGAGCCGCGAAGAAGCAGAAAATGCTGTAGGAATGATTAAAACAGCAATAAACCTCCTGCCAACTACTATACA
AGAAATCCGCATTGTTACAATTGAAAATTTAGACGAACAAGCTTGTGGAGGCACACATGTGAAAAATACGAGTGAAATAG
GAACACTTGTTATTGATAAAGTAAAAAGTAAAGGAAAGCAAAATCGTCGTTTTGAAGTTAGAGCGATTTAG

Upstream 100 bases:

>100_bases
AGTCTTTTATTAATTTTAAATTTCAGAATTATCAATTGACTTTGTGGAGCTTCTTTTTTATATTAGTAGGTATAACTATA
ACTACTAAAGAGGTGAGAGT

Downstream 100 bases:

>100_bases
ATTGTGAGGGATTACCGATGGATGAAATTATAAAGGAATTACAAAAGTTAGGATTTTCCCAGTATGAATGTAAAGCGTAT
ATTGGACTACTAAAACATTC

Product: alanine--tRNA ligase

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MTTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGFIVQDGSVFEVEKVKKEQGEIVHYIKDGAQVKLG
PVKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQIYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEIST
RYMSREEAENAVGMIKTAINLLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI

Sequences:

>Translated_236_residues
MTTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGFIVQDGSVFEVEKVKKEQGEIVHYIKDGAQVKLG
PVKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQIYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEIST
RYMSREEAENAVGMIKTAINLLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI
>Mature_235_residues
TTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGFIVQDGSVFEVEKVKKEQGEIVHYIKDGAQVKLGP
VKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQIYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEISTR
YMSREEAENAVGMIKTAINLLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=212, Percent_Identity=31.1320754716981, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI217416402, Length=212, Percent_Identity=31.1320754716981, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI109148542, Length=228, Percent_Identity=26.7543859649123, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789048, Length=212, Percent_Identity=27.8301886792453, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324288, Length=255, Percent_Identity=25.4901960784314, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18543305, Length=216, Percent_Identity=27.3148148148148, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 26645; Mature: 26514

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGFIVQDGSVFEVEKV
CCEEEEEEHHHCCCCCEEEEEEECCEEEEEEEEEEECCCCCCCCCCEEEECCCEEHHHHH
KKEQGEIVHYIKDGAQVKLGPVKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQ
HHHCCCEEEEECCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCE
IYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEISTRYMSREEAENAVGMIKTAIN
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI
HHHHHHHHEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure 
TTALYLEDAYKTSCETEVIKVEGNKVFVKETVFYPTGGGQECDTGFIVQDGSVFEVEKV
CEEEEEEHHHCCCCCEEEEEEECCEEEEEEEEEEECCCCCCCCCCEEEECCCEEHHHHH
KKEQGEIVHYIKDGAQVKLGPVKLEINWERRHNLMRHHSLLHLIGAVVYEKYGALCTGNQ
HHHCCCEEEEECCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCE
IYPDKARIDFNELQELSSVEVEGIVKEVNKLIEQNKEISTRYMSREEAENAVGMIKTAIN
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
LLPTTIQEIRIVTIENLDEQACGGTHVKNTSEIGTLVIDKVKSKGKQNRRFEVRAI
HHHHHHHHEEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726 [H]