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The map label for this gene is cls [H]

Identifier: 52143622

GI number: 52143622

Start: 1691538

End: 1692749

Strand: Direct

Name: cls [H]

Synonym: BCZK1611

Alternate gene names: 52143622

Gene position: 1691538-1692749 (Clockwise)

Preceding gene: 52143625

Following gene: 52143621

Centisome position: 31.91

GC content: 31.93

Gene sequence:

>1212_bases
ATGATTAAAAAAATATTGGGAGTTACTTCTATTATTATTGCTATCTTCGCTTTTATTTTAATTTGTATGCATATTGATGT
TACTTTAGGTAGGAAAATGGAAGCTGGGAAAAACATGCCGACAGAGTATGCACCTCATTATAGTGATTTTCAATTATATG
TAGAAGGAAAGTCATTTTATAAACAGTTATTTACTGATATAAAAGAAGCGAAACAATCTATTTACACTTATTTTTTTATT
TTATCGGATGATAAAAGTAGTCATACTTTTTTAAACTTATTAAAAGAAAAGGCAAAAGAAGGAGTAAACGTATATTTATC
TGTCGATTTACTTAACGATTTATCATTTGAAAGAAAGATGAAAAAAGAATTACAGGAAAATGGTGTACATTTTACATATA
GTAGAAAACAGGAATTACCATTCGGGTTTTATTCACTTCACCATCGTAATCATCGCCGCATTACAACGATTGATGGAGAG
ATTGGTTATACGGGTGGTTTTAATATAGGGGATGAATACTTAGGGAAAGATAAACACTTTGGGTACTGGAGAGACTATCA
TGTACGGATAAAAGGTGAAGGTGCAAAAGACTTAGAGGAACAATTTGCTTTAGATTGGAAACGAGATACGAAAGAAGATA
TAAAGAGGAGTACGAATAAGGCTAGCAAAGGGAATACACTACATACTATGGTTAGTTATAATGGGCATCAAGTGGCTGAA
AAATATATAGATCTTATAAAACAAGCTCAGCACTCAATTGTAATTGCAACGCCGTATTTTATAGCGAAAAATAAAGAATC
CATGAATGCTTTAATCGCAGCACAAAAGCGTGGTGTTACAGTAAAAATACTTTGGTCATATAAACCAGATCTACCTCTTA
TAAAAGAAGCGGCGTATCCATACATACGTCAAGCTATTAATAATGGGATTACTGTATATGGGTATAAAAAAGGAATGTTC
CATGGCAAATTCATGCTTATTGATAATGAATTAACCGTTATTGGCACAACAAACTTTACTTCGCGTAGCTTCAATATAAA
TGATGAAATGAATTTTTATATTCATGGTGGTCCTATTGTAGGGCAAGTTAATAAGGCGTTAACAGAGGATTTTCGTGAAT
CAAAAGAAATGACGAAAGAATTTTTTGAGAAGTTATCTTTTTGGGAGCGTTGTAAGGAGAAATTTGCAGGAATGGTGGAT
TTTTATTTATAA

Upstream 100 bases:

>100_bases
TGTGACGTTAAGTCGCATCCTTTTTTGTATGAAGCATGAAATCTAAATTTGAATGCCAAAATAGTAGGGTGGAAAGAAAA
ATGAGCGGAAGGAAATTAAC

Downstream 100 bases:

>100_bases
AGATTAATGGGAAAAGGGTGTCTTTTATAGACACCCTTTTGTGCATGAGAAATATTTATTTCTCATGCACAAGTAAGGAA
GCGTCATCATTAATCAACGT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MIKKILGVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQLFTDIKEAKQSIYTYFFI
LSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGE
IGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHQVAE
KYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAINNGITVYGYKKGMF
HGKFMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRESKEMTKEFFEKLSFWERCKEKFAGMVD
FYL

Sequences:

>Translated_403_residues
MIKKILGVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQLFTDIKEAKQSIYTYFFI
LSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGE
IGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHQVAE
KYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAINNGITVYGYKKGMF
HGKFMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRESKEMTKEFFEKLSFWERCKEKFAGMVD
FYL
>Mature_403_residues
MIKKILGVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFYKQLFTDIKEAKQSIYTYFFI
LSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFERKMKKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGE
IGYTGGFNIGDEYLGKDKHFGYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHQVAE
KYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYPYIRQAINNGITVYGYKKGMF
HGKFMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIVGQVNKALTEDFRESKEMTKEFFEKLSFWERCKEKFAGMVD
FYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=359, Percent_Identity=25.3481894150418, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787007, Length=369, Percent_Identity=25.2032520325203, Blast_Score=119, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 46816; Mature: 46816

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKKILGVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCHHHH
KQLFTDIKEAKQSIYTYFFILSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFERKM
HHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHHHH
KKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDKHF
HHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCC
GYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHQVAE
CCEEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
KYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYP
HHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCH
YIRQAINNGITVYGYKKGMFHGKFMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIV
HHHHHHHCCEEEEEEECCCEEEEEEEEECCEEEEECCCCCCCEECCCCCEEEEEECCCCH
GQVNKALTEDFRESKEMTKEFFEKLSFWERCKEKFAGMVDFYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MIKKILGVTSIIIAIFAFILICMHIDVTLGRKMEAGKNMPTEYAPHYSDFQLYVEGKSFY
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCEEEEECCHHHH
KQLFTDIKEAKQSIYTYFFILSDDKSSHTFLNLLKEKAKEGVNVYLSVDLLNDLSFERKM
HHHHHHHHHHHHCEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEEHHCCHHHHHH
KKELQENGVHFTYSRKQELPFGFYSLHHRNHRRITTIDGEIGYTGGFNIGDEYLGKDKHF
HHHHHHCCCEEEECCCCCCCCCHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCC
GYWRDYHVRIKGEGAKDLEEQFALDWKRDTKEDIKRSTNKASKGNTLHTMVSYNGHQVAE
CCEEEEEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCEEEEEEECCCHHHHH
KYIDLIKQAQHSIVIATPYFIAKNKESMNALIAAQKRGVTVKILWSYKPDLPLIKEAAYP
HHHHHHHHCCCCEEEECCEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCH
YIRQAINNGITVYGYKKGMFHGKFMLIDNELTVIGTTNFTSRSFNINDEMNFYIHGGPIV
HHHHHHHCCEEEEEEECCCEEEEEEEEECCEEEEECCCCCCCEECCCCCEEEEEECCCCH
GQVNKALTEDFRESKEMTKEFFEKLSFWERCKEKFAGMVDFYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]