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The map label for this gene is malS [H]

Identifier: 52143619

GI number: 52143619

Start: 1696716

End: 1698449

Strand: Direct

Name: malS [H]

Synonym: BCZK1615

Alternate gene names: 52143619

Gene position: 1696716-1698449 (Clockwise)

Preceding gene: 52143620

Following gene: 52143618

Centisome position: 32.01

GC content: 39.33

Gene sequence:

>1734_bases
ATGAATAGAGGTAGGGATGTTATGAGCAAGTTTACAGTAGCTTCTAATGGTTCATTAGAAACGACATTAAGAGGAGTAGA
AATATTATCAACACCATTTTTAAATAAAGGGGTCGCTTTCACGCAAGAAGAAAGAGAAGAATTAGGTTTAAAAGGGTTAT
TACCGCCAGCAGTTTTAACATTGGAAGAACAAGCACGCCGAGCATATGAACAATTTTGTTCTCAGCCGGATGATTTATTA
AAGAATGTATACTTAACAGCGTTACATGATCGAAATGAAGTGTTATTTTATCGTATTTTAACAGATCATTTACGCGAAAT
GTTACCAATCGTATATACACCAACTGTTGGTGTAGCGATTCAAAGATATAGTCATGAATATCGTAAACCACGAGGTGTGT
ATTTATCAATTAACGATCTGTCAGGTATTGAAGAAGCGTTTGCCAATATTGGTGCAACAGCAGAAAATATTGATTTAGTC
GTTGTAACAGATGGAGAAGGTATATTAGGAATTGGTGACTGGGGCGTTGGTGGTATTAACATCGCAATCGGAAAATTAGC
TGTTTATACAGCCGCAGTTGGAATCGATCCTAGCCGCGTATTACCGGTAATTTTAGATGTAGGTACAAATCGTGAAGAAT
TATTAGACAATCCATTTTATATTGGAAATCGTCATCCTCGTATAACAGGTGAAGCTTACGATGAATTTATTGATACATTT
GTACAAGCGGTAAATAAGCAATTCCCGAAAGCACTTTTACATTGGGAAGACTTCAGTTCTCGTAATGCACGAAAAATTTT
AGATAAATATCGTCATGATATTTGTACGTTTAATGATGATATTCAAGGTACAGGTGCAGTTTCGCTTGCGGCTGTATTAT
CGGCAGTGAAAGCTTCTGGTGTACCGCTAAGTGAACACCGCGTTGTTGTGTTTGGTGCTGGTACGGCTGGAATCGGTATC
GCAGATCAAGTTAGAGATGCGATGGTCCGCGTAGGCGTATCAGAAGAAGAATCATATAAGCGTTTCTGGTGTATTGATCG
TAATGGGTTAGTTACAGATAATATGGAAGATCTTCTTGATTTTCAAATGCCGTATGCAAGAAAAGAAGCGGAAGTAAGTG
AGTGGAAAGAAAATGGTGTGATTGGACTTGCTGAAGTTGTGAAACAAGTGAAACCGACAATTTTAATCGGTACATCAACT
GTTGCTGGTGCATTTAAAGAAGAAATTATTAAAGAAATGGCTTCTCACGTAGAAAGACCAATCATTTTACCGATGTCGAA
TCCGACGCCACTTGCTGAAGCGAAACCAGCCGATTTAATCGAGTGGACAGAAGGAAGAGCACTAGTTGCAACAGGAAGTC
CATTTGAACCAGTTACATATAACGGTGTAACGTATGTGATTGGGCAATCAAATAATGCACTTATTTTCCCAGGTCTTGGT
CTTGGAACAATTGTCGTACGTGCAAGCGTCATGACAGATGGAATGTTCGCAGCAGCAGCTGAAGCAGTTGCAAGCATGGT
GGACACAAGTCAGCCAGGAGCACCTATTTTGCCAGAAGTTGAAGAGTTACGTAATATCTCGGAAATGGTAGCAATTGAAG
TAGCGAAAGTTGCTGTTGCAGAAGGTGTTGCTAGAGAGAACTTAAGTGATAACGATATCAAGATTGCAGTGAAAGAAGCA
ATCTGGGAGCCTGAGTATCGCCAAATAAAAGCGGTAGAAAAGGTTACAATATAG

Upstream 100 bases:

>100_bases
GACACACCCAGGGATTGCAGGAGCAACTCTTTTAAAGAAAAATTAAAAGAAGAGGGGGGACAAGCCCCTCTTTTTTGTTT
ACAATATAGAAATGCTATAT

Downstream 100 bases:

>100_bases
AAATAAAGCCCGTTTATAATATAAGCGGGCTTTCACTTTTATTACTGAAAAAAAGAGTATAGGAAGTGGCACCTTTGAAT
TGGAATCAATTATGGAAGAA

Product: malate dehydrogenase

Products: NA

Alternate protein names: NAD-ME 3 [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MNRGRDVMSKFTVASNGSLETTLRGVEILSTPFLNKGVAFTQEEREELGLKGLLPPAVLTLEEQARRAYEQFCSQPDDLL
KNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDLSGIEEAFANIGATAENIDLV
VVTDGEGILGIGDWGVGGINIAIGKLAVYTAAVGIDPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTF
VQAVNKQFPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGI
ADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQMPYARKEAEVSEWKENGVIGLAEVVKQVKPTILIGTST
VAGAFKEEIIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLG
LGTIVVRASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARENLSDNDIKIAVKEA
IWEPEYRQIKAVEKVTI

Sequences:

>Translated_577_residues
MNRGRDVMSKFTVASNGSLETTLRGVEILSTPFLNKGVAFTQEEREELGLKGLLPPAVLTLEEQARRAYEQFCSQPDDLL
KNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDLSGIEEAFANIGATAENIDLV
VVTDGEGILGIGDWGVGGINIAIGKLAVYTAAVGIDPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTF
VQAVNKQFPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGI
ADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQMPYARKEAEVSEWKENGVIGLAEVVKQVKPTILIGTST
VAGAFKEEIIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLG
LGTIVVRASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARENLSDNDIKIAVKEA
IWEPEYRQIKAVEKVTI
>Mature_577_residues
MNRGRDVMSKFTVASNGSLETTLRGVEILSTPFLNKGVAFTQEEREELGLKGLLPPAVLTLEEQARRAYEQFCSQPDDLL
KNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAIQRYSHEYRKPRGVYLSINDLSGIEEAFANIGATAENIDLV
VVTDGEGILGIGDWGVGGINIAIGKLAVYTAAVGIDPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTF
VQAVNKQFPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASGVPLSEHRVVVFGAGTAGIGI
ADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLDFQMPYARKEAEVSEWKENGVIGLAEVVKQVKPTILIGTST
VAGAFKEEIIKEMASHVERPIILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLG
LGTIVVRASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVAEGVARENLSDNDIKIAVKEA
IWEPEYRQIKAVEKVTI

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malic enzymes family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=546, Percent_Identity=41.9413919413919, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI62420880, Length=546, Percent_Identity=41.9413919413919, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI239049447, Length=546, Percent_Identity=41.9413919413919, Blast_Score=431, Evalue=1e-121,
Organism=Homo sapiens, GI4505143, Length=554, Percent_Identity=40.6137184115523, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI4505145, Length=571, Percent_Identity=39.2294220665499, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI270265879, Length=482, Percent_Identity=41.701244813278, Blast_Score=363, Evalue=1e-100,
Organism=Escherichia coli, GI87081919, Length=555, Percent_Identity=46.6666666666667, Blast_Score=506, Evalue=1e-144,
Organism=Caenorhabditis elegans, GI17537199, Length=552, Percent_Identity=43.1159420289855, Blast_Score=436, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322823, Length=578, Percent_Identity=44.9826989619377, Blast_Score=502, Evalue=1e-143,
Organism=Drosophila melanogaster, GI281362674, Length=550, Percent_Identity=40.3636363636364, Blast_Score=421, Evalue=1e-118,
Organism=Drosophila melanogaster, GI281362672, Length=550, Percent_Identity=40.3636363636364, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI21356279, Length=550, Percent_Identity=40.3636363636364, Blast_Score=420, Evalue=1e-117,
Organism=Drosophila melanogaster, GI24646386, Length=527, Percent_Identity=39.8481973434535, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24646388, Length=527, Percent_Identity=39.8481973434535, Blast_Score=394, Evalue=1e-109,
Organism=Drosophila melanogaster, GI78707242, Length=543, Percent_Identity=35.7274401473296, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI78707238, Length=543, Percent_Identity=35.7274401473296, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI281363505, Length=538, Percent_Identity=34.7583643122677, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI78707236, Length=538, Percent_Identity=34.7583643122677, Blast_Score=359, Evalue=3e-99,
Organism=Drosophila melanogaster, GI281363503, Length=536, Percent_Identity=34.5149253731343, Blast_Score=355, Evalue=6e-98,
Organism=Drosophila melanogaster, GI78707240, Length=519, Percent_Identity=36.4161849710983, Blast_Score=354, Evalue=1e-97,
Organism=Drosophila melanogaster, GI78707232, Length=512, Percent_Identity=35.546875, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI19922384, Length=573, Percent_Identity=28.7958115183246, Blast_Score=256, Evalue=3e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR001891
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M [H]

EC number: =1.1.1.38 [H]

Molecular weight: Translated: 63116; Mature: 63116

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRGRDVMSKFTVASNGSLETTLRGVEILSTPFLNKGVAFTQEEREELGLKGLLPPAVLT
CCCCHHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHH
LEEQARRAYEQFCSQPDDLLKNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAI
HHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHCCEEECCCHHHHH
QRYSHEYRKPRGVYLSINDLSGIEEAFANIGATAENIDLVVVTDGEGILGIGDWGVGGIN
HHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCEE
IAIGKLAVYTAAVGIDPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTF
EEHHHHEEHHHHCCCCHHHCEEEEEECCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
VQAVNKQFPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
VPLSEHRVVVFGAGTAGIGIADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLD
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHH
FQMPYARKEAEVSEWKENGVIGLAEVVKQVKPTILIGTSTVAGAFKEEIIKEMASHVERP
HCCCCCCCCCHHHHHHHCCCEEHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
IILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLG
EEEECCCCCCCCCCCCCCCEEECCCCEEEECCCCCCCEEECCEEEEEECCCCEEEECCCC
LGTIVVRASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EGVARENLSDNDIKIAVKEAIWEPEYRQIKAVEKVTI
HHHHHCCCCCCCEEEEEHHHHCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MNRGRDVMSKFTVASNGSLETTLRGVEILSTPFLNKGVAFTQEEREELGLKGLLPPAVLT
CCCCHHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCHHHHH
LEEQARRAYEQFCSQPDDLLKNVYLTALHDRNEVLFYRILTDHLREMLPIVYTPTVGVAI
HHHHHHHHHHHHHCCHHHHHHHHHHEEEECCCCEEEHHHHHHHHHHHCCEEECCCHHHHH
QRYSHEYRKPRGVYLSINDLSGIEEAFANIGATAENIDLVVVTDGEGILGIGDWGVGGIN
HHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECCCCEEEECCCCCCCEE
IAIGKLAVYTAAVGIDPSRVLPVILDVGTNREELLDNPFYIGNRHPRITGEAYDEFIDTF
EEHHHHEEHHHHCCCCHHHCEEEEEECCCCHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
VQAVNKQFPKALLHWEDFSSRNARKILDKYRHDICTFNDDIQGTGAVSLAAVLSAVKASG
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
VPLSEHRVVVFGAGTAGIGIADQVRDAMVRVGVSEEESYKRFWCIDRNGLVTDNMEDLLD
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHHHH
FQMPYARKEAEVSEWKENGVIGLAEVVKQVKPTILIGTSTVAGAFKEEIIKEMASHVERP
HCCCCCCCCCHHHHHHHCCCEEHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHCCCC
IILPMSNPTPLAEAKPADLIEWTEGRALVATGSPFEPVTYNGVTYVIGQSNNALIFPGLG
EEEECCCCCCCCCCCCCCCEEECCCCEEEECCCCCCCEEECCEEEEEECCCCEEEECCCC
LGTIVVRASVMTDGMFAAAAEAVASMVDTSQPGAPILPEVEELRNISEMVAIEVAKVAVA
HHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EGVARENLSDNDIKIAVKEAIWEPEYRQIKAVEKVTI
HHHHHCCCCCCCEEEEEHHHHCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]