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The map label for this gene is vanS [H]

Identifier: 52143544

GI number: 52143544

Start: 1784693

End: 1785820

Strand: Direct

Name: vanS [H]

Synonym: BCZK1690

Alternate gene names: 52143544

Gene position: 1784693-1785820 (Clockwise)

Preceding gene: 52143545

Following gene: 52143543

Centisome position: 33.67

GC content: 30.05

Gene sequence:

>1128_bases
TTGAAGAGAATCCAAAAGAACCGAAGTTTATTCAAAATGTTTGGGGAGTCGGATATAAAATTGAAGATTAAATCATTACA
AGGCATTAGAGCTAAGTTTTTTATCGCATTCATATGTAGCATCTTGTTAGCAACTATAAGTATAATAGTGTTTCAAATTT
TAATTGGAAATATATATAGTCATGTTACTACGTTAGAGGAAAAATATTCATTTTTATATTTTATAGTTTTTTTGATTTTT
ACTACGGCATACTTTGCATGTATGACAAAAACAATGATGAAAAGATTGTCTGAAATTAATAAGAACGTGAAGGAAATAAG
TAAGGGTAATTTTGAAGTATATATACCTATTTCAAAAAATGACGAGATTGGTGAATTAGCGACGAATGTAAATAGAATGG
CCAAAAGTTTAAAAGAGTCTGTCGAGAATGAAAAAAAATCACAGGAAATGAAAAATGAAATGATTAGTAATATTTCGCAT
GACTTACGAACACCTGTAACATCTTTAATCGGTTATGCGGACTTATTAGGAAATAAGCTGCATGCAAATGGAGAAGAATG
TGAACAGTATGTAAGCATATTAAAGCGAAAAAGTTATGAATTAAAAAATCAAGTAGATGAATTATTAGAATACTGTCAAA
TAAATTATAGAGAGATTGAATTACATAAAAGTCTAGTAAATATGAAAGCGTTAATGGAACAAATTATGATTGATTTTGTA
CCACAACTAGATGATGCTAATATGAGCTTTTGTATAAAAGGTGATAAGGAGTTACATGTGGAAATAGATGTAGCGCTTAT
GGTGAGGTTATTCGAAAATGTAATTAGTAATAGTATTATGTACGGGAAAGACGGAAAGGAGATTTTAATTCTAGTTTCTA
AAAGAGACATGAATGTTGAAATAGCAATTAAAAATTTTGGACAATGTATTCCAGATGAGAACCTTCCTTACGTTTTTGAG
AAGTTTTATCGTGGTGAAAAATCACGTAGCTCCCATACAGGTGGAAAAGGAATGGGACTTGCAATTGCGAAAAGTATAGC
AGAACTTCATAAAGGAGATATCGCTGTACGTAGTAACGATAAAGAAACAGTTTTTACTATCGTTTTACCGCAGTATAAAG
AAATGTAA

Upstream 100 bases:

>100_bases
GTATTTACTATTGAAGAAATATATGAGAGCGTTTGGAAAGAAAGGGCTTATGAAAGCAATAATACAGTAATGGTTCATAT
CGCAAGATTAAGAAATAAAA

Downstream 100 bases:

>100_bases
GAAAGTCGTAAGTATTTCTTACGTATGTTGTAATTTTCAATTCGTATCATGTAGAACAAAGATATGAGAGTGGGGATATA
ACATGTGGAAGAAATATGTG

Product: sensor histidine kinase

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKRIQKNRSLFKMFGESDIKLKIKSLQGIRAKFFIAFICSILLATISIIVFQILIGNIYSHVTTLEEKYSFLYFIVFLIF
TTAYFACMTKTMMKRLSEINKNVKEISKGNFEVYIPISKNDEIGELATNVNRMAKSLKESVENEKKSQEMKNEMISNISH
DLRTPVTSLIGYADLLGNKLHANGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSLVNMKALMEQIMIDFV
PQLDDANMSFCIKGDKELHVEIDVALMVRLFENVISNSIMYGKDGKEILILVSKRDMNVEIAIKNFGQCIPDENLPYVFE
KFYRGEKSRSSHTGGKGMGLAIAKSIAELHKGDIAVRSNDKETVFTIVLPQYKEM

Sequences:

>Translated_375_residues
MKRIQKNRSLFKMFGESDIKLKIKSLQGIRAKFFIAFICSILLATISIIVFQILIGNIYSHVTTLEEKYSFLYFIVFLIF
TTAYFACMTKTMMKRLSEINKNVKEISKGNFEVYIPISKNDEIGELATNVNRMAKSLKESVENEKKSQEMKNEMISNISH
DLRTPVTSLIGYADLLGNKLHANGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSLVNMKALMEQIMIDFV
PQLDDANMSFCIKGDKELHVEIDVALMVRLFENVISNSIMYGKDGKEILILVSKRDMNVEIAIKNFGQCIPDENLPYVFE
KFYRGEKSRSSHTGGKGMGLAIAKSIAELHKGDIAVRSNDKETVFTIVLPQYKEM
>Mature_375_residues
MKRIQKNRSLFKMFGESDIKLKIKSLQGIRAKFFIAFICSILLATISIIVFQILIGNIYSHVTTLEEKYSFLYFIVFLIF
TTAYFACMTKTMMKRLSEINKNVKEISKGNFEVYIPISKNDEIGELATNVNRMAKSLKESVENEKKSQEMKNEMISNISH
DLRTPVTSLIGYADLLGNKLHANGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSLVNMKALMEQIMIDFV
PQLDDANMSFCIKGDKELHVEIDVALMVRLFENVISNSIMYGKDGKEILILVSKRDMNVEIAIKNFGQCIPDENLPYVFE
KFYRGEKSRSSHTGGKGMGLAIAKSIAELHKGDIAVRSNDKETVFTIVLPQYKEM

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=287, Percent_Identity=25.7839721254355, Blast_Score=113, Evalue=3e-26,
Organism=Escherichia coli, GI1786783, Length=276, Percent_Identity=25.7246376811594, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=26.1261261261261, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786912, Length=236, Percent_Identity=25.8474576271186, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI145693157, Length=244, Percent_Identity=23.7704918032787, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787894, Length=278, Percent_Identity=24.4604316546763, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1789149, Length=256, Percent_Identity=23.828125, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082128, Length=238, Percent_Identity=24.3697478991597, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788279, Length=310, Percent_Identity=24.1935483870968, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI48994928, Length=235, Percent_Identity=25.1063829787234, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790346, Length=308, Percent_Identity=21.7532467532468, Blast_Score=69, Evalue=7e-13,
Organism=Escherichia coli, GI1790436, Length=243, Percent_Identity=24.2798353909465, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788549, Length=222, Percent_Identity=23.8738738738739, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790861, Length=227, Percent_Identity=21.5859030837004, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1790551, Length=305, Percent_Identity=21.9672131147541, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788713, Length=249, Percent_Identity=22.8915662650602, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43069; Mature: 43069

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRIQKNRSLFKMFGESDIKLKIKSLQGIRAKFFIAFICSILLATISIIVFQILIGNIYS
CCCHHHHHHHHHHHCCCCCEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVTTLEEKYSFLYFIVFLIFTTAYFACMTKTMMKRLSEINKNVKEISKGNFEVYIPISKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
DEIGELATNVNRMAKSLKESVENEKKSQEMKNEMISNISHDLRTPVTSLIGYADLLGNKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HANGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSLVNMKALMEQIMIDFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PQLDDANMSFCIKGDKELHVEIDVALMVRLFENVISNSIMYGKDGKEILILVSKRDMNVE
CCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCCEE
IAIKNFGQCIPDENLPYVFEKFYRGEKSRSSHTGGKGMGLAIAKSIAELHKGDIAVRSND
EEEHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCC
KETVFTIVLPQYKEM
CCEEEEEECCHHCCC
>Mature Secondary Structure
MKRIQKNRSLFKMFGESDIKLKIKSLQGIRAKFFIAFICSILLATISIIVFQILIGNIYS
CCCHHHHHHHHHHHCCCCCEEEEHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HVTTLEEKYSFLYFIVFLIFTTAYFACMTKTMMKRLSEINKNVKEISKGNFEVYIPISKN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
DEIGELATNVNRMAKSLKESVENEKKSQEMKNEMISNISHDLRTPVTSLIGYADLLGNKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HANGEECEQYVSILKRKSYELKNQVDELLEYCQINYREIELHKSLVNMKALMEQIMIDFV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
PQLDDANMSFCIKGDKELHVEIDVALMVRLFENVISNSIMYGKDGKEILILVSKRDMNVE
CCCCCCCCEEEEECCCEEEEEHHHHHHHHHHHHHHCCCEEECCCCCEEEEEEECCCCCEE
IAIKNFGQCIPDENLPYVFEKFYRGEKSRSSHTGGKGMGLAIAKSIAELHKGDIAVRSND
EEEHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCC
KETVFTIVLPQYKEM
CCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]