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The map label for this gene is bacT [H]

Identifier: 52143521

GI number: 52143521

Start: 1811625

End: 1812347

Strand: Direct

Name: bacT [H]

Synonym: BCZK1713

Alternate gene names: 52143521

Gene position: 1811625-1812347 (Clockwise)

Preceding gene: 52143522

Following gene: 52143519

Centisome position: 34.18

GC content: 33.2

Gene sequence:

>723_bases
ATGAATAAAATTAAACTTTTCTGTCTTCCATATGCAGGAGGAACAGCAAATATATATATTAAATGGAGGAGGTATTTACT
TCCCGAAATCGAACTTATTCCGCTAGAGTTAGCGGGTAGGGGAAAGAGAATAGGGGACTTGTTGTATAGCGATATGAATG
AAGCCATTGAAGATATATATCAAACAATCAAACCTCATTTGGATGGTACGCCATATGCTTTCTGGGGACATAGTATGGGG
AGTTTACTTGCTTACGAGTGCACTCATAAAATTTTAGAACAGCAGCATAAGGGGCCGGAGACAATCTTCTTTTCAGGAAA
AAACCCTCCTCATATCAAGGTGAAAAAAATAAGGCACTTATTACCTAATAGGGAATTTATTGATGAAGTTTTTTCTATGG
GAGGGACCTCAAAAGAACTGATTGAGCAAGAAGATTTATTAGATTTCTTTATACCGATACTAAGAGCTGATTTTAGATTA
GTCGAGACATATCAATATTGTAGAAAAAAGAAATTTTCATTTGATATTGCAGTTTTGAATGGGATAGAGGATGAAATGAC
AACAAAAATAGAAATAGAAGAATGGACTAAATATACAGAGAAACAGTGTGATATTTATCAATTGAATGGTGCCCATTTCT
TTATTCATGATCAAATGAATGATGTAATTAATATAATTAATAATAAATTGCGTCAATATACTTTAGAGTTAGCTAGGTGT
TAG

Upstream 100 bases:

>100_bases
GAAAATAATAATCAGCTTAATGATGATATATTTTCGTTTATAGAAAGTAACTAGAATAATTAAATTGTTAATTTTCGTAA
TTTATTGAAGGAGATATCTT

Downstream 100 bases:

>100_bases
CAGAATTTATGATGAAACAGTTGGGAAAACTAGAGTATTACAAGTTTTTTTATTTAGTAATTAGTAAAAACAGTAAGAAA
CACAGGGCGAAAAACTCTGT

Product: thioesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MNKIKLFCLPYAGGTANIYIKWRRYLLPEIELIPLELAGRGKRIGDLLYSDMNEAIEDIYQTIKPHLDGTPYAFWGHSMG
SLLAYECTHKILEQQHKGPETIFFSGKNPPHIKVKKIRHLLPNREFIDEVFSMGGTSKELIEQEDLLDFFIPILRADFRL
VETYQYCRKKKFSFDIAVLNGIEDEMTTKIEIEEWTKYTEKQCDIYQLNGAHFFIHDQMNDVINIINNKLRQYTLELARC

Sequences:

>Translated_240_residues
MNKIKLFCLPYAGGTANIYIKWRRYLLPEIELIPLELAGRGKRIGDLLYSDMNEAIEDIYQTIKPHLDGTPYAFWGHSMG
SLLAYECTHKILEQQHKGPETIFFSGKNPPHIKVKKIRHLLPNREFIDEVFSMGGTSKELIEQEDLLDFFIPILRADFRL
VETYQYCRKKKFSFDIAVLNGIEDEMTTKIEIEEWTKYTEKQCDIYQLNGAHFFIHDQMNDVINIINNKLRQYTLELARC
>Mature_240_residues
MNKIKLFCLPYAGGTANIYIKWRRYLLPEIELIPLELAGRGKRIGDLLYSDMNEAIEDIYQTIKPHLDGTPYAFWGHSMG
SLLAYECTHKILEQQHKGPETIFFSGKNPPHIKVKKIRHLLPNREFIDEVFSMGGTSKELIEQEDLLDFFIPILRADFRL
VETYQYCRKKKFSFDIAVLNGIEDEMTTKIEIEEWTKYTEKQCDIYQLNGAHFFIHDQMNDVINIINNKLRQYTLELARC

Specific function: Probable thioesterase involved in the biosynthesis of gramicidin S [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=225, Percent_Identity=29.7777777777778, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI8922871, Length=195, Percent_Identity=29.7435897435897, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 28188; Mature: 28188

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIKLFCLPYAGGTANIYIKWRRYLLPEIELIPLELAGRGKRIGDLLYSDMNEAIEDIY
CCCEEEEEEECCCCCEEEEEEEEHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
QTIKPHLDGTPYAFWGHSMGSLLAYECTHKILEQQHKGPETIFFSGKNPPHIKVKKIRHL
HHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
LPNREFIDEVFSMGGTSKELIEQEDLLDFFIPILRADFRLVETYQYCRKKKFSFDIAVLN
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GIEDEMTTKIEIEEWTKYTEKQCDIYQLNGAHFFIHDQMNDVINIINNKLRQYTLELARC
CCCHHHEEEEEHHHHHHHHHCCCCEEEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIKLFCLPYAGGTANIYIKWRRYLLPEIELIPLELAGRGKRIGDLLYSDMNEAIEDIY
CCCEEEEEEECCCCCEEEEEEEEHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHH
QTIKPHLDGTPYAFWGHSMGSLLAYECTHKILEQQHKGPETIFFSGKNPPHIKVKKIRHL
HHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHH
LPNREFIDEVFSMGGTSKELIEQEDLLDFFIPILRADFRLVETYQYCRKKKFSFDIAVLN
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
GIEDEMTTKIEIEEWTKYTEKQCDIYQLNGAHFFIHDQMNDVINIINNKLRQYTLELARC
CCCHHHEEEEEHHHHHHHHHCCCCEEEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2477357; 7512553 [H]