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The map label for this gene is topB [H]

Identifier: 52143516

GI number: 52143516

Start: 1846343

End: 1848487

Strand: Direct

Name: topB [H]

Synonym: BCZK1725

Alternate gene names: 52143516

Gene position: 1846343-1848487 (Clockwise)

Preceding gene: 52143515

Following gene: 52143508

Centisome position: 34.83

GC content: 36.88

Gene sequence:

>2145_bases
ATGAAATTAATTATTGCCGAGAAACCAGATCAAGGTTTGGCTCTTGTTTCACAGTTTAAATATCGCCGGAAAGATGGATA
TTTAGAAGTAGAAGCGAATGAGTTATTTCCAAGTGGAGCGTACTGTACATGGGCAATTGGTCATTTGACGCAGTTATGTA
ATCCAGAACATTATCACGCAGAGTGGAAAAAATGGTCACTTGATACGTTACCGATGATTCCTGAACGATTTCAATTTGAA
GTAACAAAGTCGAAGTATAAACAATTTAACGTTGTGAAACAGCTGTTACATAATCCTCAGGTAACAGAAATTATTCATGC
GGGCGATGCTGGGCGTGAAGGGGAATTGATTGTACGAAATATTATTAACCTTTGCAACGTGCAAAAGCCGATGAAACGTC
TTTGGATTTCATCTTTAACGAAACAAGCTATTTATCAAGGCTTTAAAAACTTACTCGATGAATCAGATACAATCAATACG
TACTATGAGGCGTATACAAGGTCGTGTGCGGACTGGGTCGTTGGGATGAATGCATCGCGTGTGTTTAGTATTTTGCTAAA
GAAAAAAGGAATGAACGATGTATTTTCAGCTGGTCGTGTACAAACACCGACACTCGCGTTAATCGTAAAGCGTGAGAAAG
AAATTGAAAACTTTAAGTCAGAGCCGTTTTGGGAAGTGTTCGCAACCTTTAATATAGAAGGAAAGAAATATGATGGGAAA
TGGGAAAAGGATAATGAATCCCGCTTACAAGACCCTGATATGGCAAATAAAATTGCGGCATTTTGCCAAGGAAAACCGGC
GGTAGTAAAGGAAATGAAAACGGAGCGTAAAGAGTTTCAGCCTCCGCTTTTATTTAACTTATCATCACTACAAGCAACGG
CAAATAAAGCATTTAAATTTTCACCGAAAAAGACGCTTGATATAACGCAAGCACTATATCAAAAAGGAATTGTCTCTTAT
CCTCGTTCAGATTCCAACTATGTTACACAAGGTGAAGCGGCAACATTCCCTGATATTTTACAGAAGTTAAGTCAGTTTGA
TGAATATAAAGGTTTATTACCAGCTCCGGTTGAATCGATTATGAATAATAAGCGTTATGTGAATGAAAAGAAAGTAACGG
ATCACTACGCCATTATTCCGACAGAGCAAGTTACAAATCCAAGCAGACTATCAGGTGATGAAAAGAAAATTTACGATATG
ATCGTAAGAAGGCTTATTGCAGCTCACTATGAAGTTGCAATCTTTGACTATACAACGATTGTAACGCTTGTAGATGAACG
TGCTGAATTCATTTCAAAAGGAAAACAGCAAATTCAAGAAGGATGGCGTAAAGTTATTTTCCAAGACGATAAAGATGATG
AAACCATTCTTCCAATTGTAGCTGAAGGTGAAGAAGGAAAAGTTGTAAAGGTGAAAGTGAAAGAAGGAAAAACACAACCA
CCGAAGCGTTATACAGAAGGACAACTTATTACGTTAATGAAAACAGCTGGTAAATATTTAGAGAATGAAGAGCTGGAGAA
AGTATTAAAGAAAACAGAAGGTTTAGGTACAGAAGCAACTCGTGCAGGTATTATTACGATGCTAAAAGACCGTAAATATA
TAGATGTGAAAAAAAACCAGGTGTATGCGACTGATAAAGGAAAAGTATTAATTACCGCAATCGGTGACAAAATACTAGCT
TCACCAGAAATGACCGCAAAATGGGAGCAACGTCTTGCGGAAATTGGGGAAGGTACAGCATCACCGGCAACTTTTATGGA
ACAAACGAAAAAATTATCAGCTAAAATTATTGAAGATGCGGTTGAAATGTCTGAGAAGTGGGATTTCACCGGATTACATG
TTGAATCGATTGAGCGAAAAGGATCGAAATTTACAACGGGTAAAAAGGTTGGTAGCTGTAAAAAATGTGATGGCGATGTA
ATTGATAAGTCAACGTTTTACGGCTGTTCTAATTACAACACGACACAATGTGACTTTACCATTTCGAAGAAGATATTAAG
TAAAACAATTTCGCAAAAGAATATGACAAAGCTCTTAAAAGGTGAAAAGACGGATTTAATTAAAGGCTTTAAAAAGGGCG
AGAAAACATTTGATGCGAAGTTAGAGTGGAAAGATAATAAGATTAATTTTGTATTTGAGAATTAA

Upstream 100 bases:

>100_bases
GAGACAAACCGCGAATGATAAATAAGATTGTTGTTTTATCATCGCTTCTAAACAGATAGACAATGAAAGTAACAGTGGAA
TACAGATAGGGAGTTATAAA

Downstream 100 bases:

>100_bases
GTTGTTTCAACAAATAAAAAAATATGATAATTAAACTGTATCCTATAGAATAGACGCTGAAAAAAGTCTATTCTATAGGA
TATTTTTTTGAATAAGGAGT

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 714; Mature: 714

Protein sequence:

>714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPSGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
WEKDNESRLQDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN

Sequences:

>Translated_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPSGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
WEKDNESRLQDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
>Mature_714_residues
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPSGAYCTWAIGHLTQLCNPEHYHAEWKKWSLDTLPMIPERFQFE
VTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRNIINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINT
YYEAYTRSCADWVVGMNASRVFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
WEKDNESRLQDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKFSPKKTLDITQALYQKGIVSY
PRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESIMNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDM
IVRRLIAAHYEVAIFDYTTIVTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQVYATDKGKVLITAIGDKILA
SPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDAVEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDV
IDKSTFYGCSNYNTTQCDFTISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=673, Percent_Identity=24.2199108469539, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI4507635, Length=458, Percent_Identity=25.764192139738, Blast_Score=106, Evalue=9e-23,
Organism=Escherichia coli, GI1788061, Length=619, Percent_Identity=33.6025848142165, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1787529, Length=518, Percent_Identity=28.1853281853282, Blast_Score=134, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17555378, Length=626, Percent_Identity=27.7955271565495, Blast_Score=158, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI32563869, Length=554, Percent_Identity=24.9097472924188, Blast_Score=115, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=629, Percent_Identity=25.7551669316375, Blast_Score=103, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585251, Length=702, Percent_Identity=24.5014245014245, Blast_Score=140, Evalue=4e-33,
Organism=Drosophila melanogaster, GI24640096, Length=493, Percent_Identity=23.9350912778905, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 81476; Mature: 81476

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPSGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHHHCCCCHHCC
EWKKWSLDTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
HHHCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDNESRLQDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
HCCCHHCCCHHCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHH
VTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDVIDKSTFYGCSNYNTTQCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEECHH
ISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCEEEEEECC
>Mature Secondary Structure
MKLIIAEKPDQGLALVSQFKYRRKDGYLEVEANELFPSGAYCTWAIGHLTQLCNPEHYHA
CEEEEECCCCCCHHHHHHHHHHHCCCCEEEEHHHCCCCCCHHHHHHHHHHHHCCCCHHCC
EWKKWSLDTLPMIPERFQFEVTKSKYKQFNVVKQLLHNPQVTEIIHAGDAGREGELIVRN
HHHCCCCCCCCCCCCHHEEHHHHHHHHHHHHHHHHHCCCCHHHHEECCCCCCCCCHHHHH
IINLCNVQKPMKRLWISSLTKQAIYQGFKNLLDESDTINTYYEAYTRSCADWVVGMNASR
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHH
VFSILLKKKGMNDVFSAGRVQTPTLALIVKREKEIENFKSEPFWEVFATFNIEGKKYDGK
HHHHHHHHCCCHHHHHCCCCCCCEEEEEEECHHHHHHCCCCCCEEEEEEEEECCCEECCC
WEKDNESRLQDPDMANKIAAFCQGKPAVVKEMKTERKEFQPPLLFNLSSLQATANKAFKF
CCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCEEC
SPKKTLDITQALYQKGIVSYPRSDSNYVTQGEAATFPDILQKLSQFDEYKGLLPAPVESI
CCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHH
MNNKRYVNEKKVTDHYAIIPTEQVTNPSRLSGDEKKIYDMIVRRLIAAHYEVAIFDYTTI
HCCCHHCCCHHCCCCEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHH
VTLVDERAEFISKGKQQIQEGWRKVIFQDDKDDETILPIVAEGEEGKVVKVKVKEGKTQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCC
PKRYTEGQLITLMKTAGKYLENEELEKVLKKTEGLGTEATRAGIITMLKDRKYIDVKKNQ
CCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEECCE
VYATDKGKVLITAIGDKILASPEMTAKWEQRLAEIGEGTASPATFMEQTKKLSAKIIEDA
EEEECCCCEEEEEECCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
VEMSEKWDFTGLHVESIERKGSKFTTGKKVGSCKKCDGDVIDKSTFYGCSNYNTTQCDFT
HHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCEECHH
ISKKILSKTISQKNMTKLLKGEKTDLIKGFKKGEKTFDAKLEWKDNKINFVFEN
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1329032 [H]